Publications

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genetic differentiation and history of populations of the italian treefrog hyla intermedia: lack of concordance between mitochondrial and nuclear markers.the genetic differentiation among 33 populations of the italian treefrog, hyla intermedia (anura: hylidae), was investigated using both biparentally (23 allozyme loci) and maternally (partial mitochondrial cytochrome b gene) inherited markers. two main population groups were evidenced by both markers, located north and south of the northern apennines. however, the pattern of differentiation between these two groups was much less pronounced at allozymes than at mtdna, leading to gene flow estimat ...200717061145
phylogeography and historical demography of the italian treefrog, hyla intermedia, reveals multiple refugia, population expansions and secondary contacts within peninsular italy.we investigated the geographical patterns of genetic diversity in the italian treefrog through sequence analysis of a mitochondrial cytochrome b gene fragment. three main mitochondrial lineages were identified, distributed in northern, central and southern italy, respectively. their divergence appears indicative of a split time largely predating late pleistocene climatic oscillations, and syntopy between them was only observed in the geographically intermediate populations. the historical demogr ...200717903181
tissues injury and pathological changes in hyla intermedia juveniles after chronic larval exposure to tebuconazole.tebuconazole (tbz), an azole pesticide, is one of the most frequently detected fungicides in surface water. despite its harmful effects, mainly related to endocrine disturbance, the consequences of tbz exposure in amphibians remain poorly understood. here, we investigated the adverse and delayed effects of tbz chronic exposure on a native anuran species, often inhabiting cultivated areas, the italian tree frog (hyla intermedia). to disclose the multiple mechanisms of action through which tbz exe ...202032971454
chronic exposures to fungicide pyrimethanil: multi-organ effects on italian tree frog (hyla intermedia).amphibian habitats are easily contaminated by several pollutants, and in agricultural landscapes the likely exposure scenario is represented by pesticides. many of these substances are known or suspected to act as endocrine disrupting chemicals (edcs). the goal of the present study was to assess the effects of pyrimethanil, a common-used but also overlooked fungicide, on liver, kidney and gonadal differentiation of hyla intermedia. through a multi-organ evaluation, we demonstrated that a long te ...201728761072
effects of long-term exposure to two fungicides, pyrimethanil and tebuconazole, on survival and life history traits of italian tree frog (hyla intermedia).over the last few years, the hazards associated with the extensive use of fungicides have become an issue of great concern but, at present, the effects of these substances on amphibians remain poorly understood. the goal of the present study was to assess the effects of two commonly used fungicides, tebuconazole and pyrimethanil, on italian tree frog (hyla intermedia), a species frequently found in agricultural areas. tadpoles were exposed to fungicides from developmental gosner stage 25 (gs 25) ...201626771902
factors affecting variation in the reproductive investment of female treefrogs, hyla intermedia.in anurans, fecundity (clutch size) is the most important determinant of female reproductive success. we investigated three possible causes responsible for fecundity variation in female italian treefrogs, hyla intermedia, during four breeding seasons: (i) variation in morphological (body size and condition) and life-history (age) traits; (ii) variation in the tradeoff between the number and the size of eggs; (iii) seasonal effects and within-season differences in the timing of deposition. at the ...201223022366
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