| biosynthesis and degradation of nodule-specific rhizobium loti compounds in lotus nodules. | two nodule-specific rhizobium loti compounds were identified in lotus tenuis and lotus pedunculatus nodules induced by strain nzp2037. one, a silver nitrate-positive cation called rhizolotine, has been characterized as the riboside of a novel alpha-hydroxyimino acid containing a 1,4,5,6-tetrahydropyrimidine ring (g. j. shaw, r. d. wilson, g. a. lane, l. d. kennedy, d. b. scott, and g. j. gainsford, j. chem. soc. chem. commun., p. 180-181, 1986), and the other, yellow-1, stains yellow with ninhyd ... | 1987 | 3025173 |
| characterization of rhizobium loti strains from the salado river basin. | thirty indigenous rhizobia strains, isolated from lotus tenuis in the area of chascomús and other regions of the salado river basin (argentina), were characterized based on generation time, acid production, carbon utilization, protein profile, and molecular characterization by restriction fragment length polymorphism (rflp) analysis of 16s rrna genes amplified by the polymerase chain reaction (pcr). the results indicated that native rhizobia isolates from the chascomús area are predominantly fas ... | 2001 | 11519997 |
| analysis of mesorhizobium loti glycogen operon: effect of phosphoglucomutase (pgm) and glycogen synthase (g/ga) null mutants on nodulation of lotus tenuis. | the phosphoglucomutase (pgm) gene codes for a key enzyme required for the formation of udp-glucose and adp-glucose, the sugar donors for the biosynthesis of glucose containing polysaccharides. a mesorhizobium loti pgm null mutant obtained in this study contains an altered form of lipopolysaccharide (lps), lacks exopolysaccharide (eps), beta cyclic glucan, and glycogen and is unable to nodulate lotus tenuis. the nonnodulating phenotype of the pgm mutant was not due to the absence of glycogen, sin ... | 2002 | 12026175 |
| genetic diversity and host range of rhizobia nodulating lotus tenuis in typical soils of the salado river basin (argentina). | a total of 103 root nodule isolates were used to estimate the diversity of bacteria nodulating lotus tenuis in typical soils of the salado river basin. a high level of genetic diversity was revealed by repetitive extragenic palindromic pcr, and 77 isolates with unique genomic fingerprints were further differentiated into two clusters, clusters a and b, after 16s rrna restriction fragment length polymorphism analysis. cluster a strains appeared to be related to the genus mesorhizobium, whereas cl ... | 2009 | 19074602 |
| comparative symbiotic performance of native rhizobia of the flooding pampa and strains currently used for inoculating lotus tenuis in this region. | the flooding pampa (fp) is the most important area for cattle breeding in argentina. in this region, persistence and yield of typical forage legumes are strongly limited by soil salinity and alkalinity, which affect around 30% of the total area. instead, naturalized lotus tenuis is the main forage legume in this region. rhizobial strains currently used for inoculating l. tenuis in the fp are exotic or native from non-saline soils of this region, their taxonomic identity being unknown. assuming t ... | 2011 | 20811776 |
| phosphate-solubilization mechanism and in vitro plant growth promotion activity mediated by pantoea eucalypti isolated from lotus tenuis rhizosphere in the salado river basin (argentina). | to isolate and characterize phosphate-solubilizing strains from a constrained environment such as the salado river basin and to assess their phosphate-solubilizing mechanisms, to further selection of the most promising strains to inoculate and improve the implantation and persistence of lotus tenuis in the most important area devoted to meat-cow production in argentina. | 2011 | 21299771 |
| phylogenetic relationship of lotus uliginosus symbionts with bradyrhizobia nodulating genistoid legumes. | lotus species are legumes with potential for pastures in soils with low-fertility and environmental constraints. the aim of this work was to characterize bacteria that establish efficient nitrogen-fixing symbiosis with the forage species lotus uliginosus. a total of 39 isolates were obtained from nodules of l. uliginosus naturally growing in two different locations of portugal. molecular identification of the isolates plus the commercial inoculant strain nzp2039 was performed by rep-pcr, 16s rrn ... | 2011 | 22092879 |
| molecular characterisation of ltchi7, a gene encoding a class iii endochitinase induced by drought stress in lotus spp. | chitinases are enzymes that digest chitin molecules, present principally in insects and fungi. in plants, these enzymes play an important role in defence against pathogen attack, although they have also been described as induced by mechanical damage, ozone, heavy metals, cold, salinity, etc. using an annealing control primer, we isolated a gene fragment whose translated sequence has high homology with a class iii endochitinase. the gene, named ltchi7, consisted of one orf of 1005 bp, which codes ... | 2011 | 21143727 |
| dual effect of mesorhizobium loti t3ss functionality on the symbiotic process. | mesorhizobium loti maff303099 has a functional type iii secretory system (t3ss) involved in the nodulation process on lotus tenuis and lotus japonicus. four putative m. loti t3ss effectors (mlr6358, mlr6331, mlr6361, and mlr6316) have been previously described, and it has been demonstrated that the n-terminal regions of mlr6361 and mlr6358 mediate the secretion via a t3ss. here, we demonstrate the capacity of mlr6316 and mlr6331 n-terminal regions to direct the secretion of a translational fusio ... | 2012 | 22428564 |
| potential biocontrol actinobacteria: rhizospheric isolates from the argentine pampas lowlands legumes. | control of fungal plant diseases by using naturally occurring non-pathogenic microorganisms represents a promising approach to biocontrol agents. this study reports the isolation, characterization, and fungal antagonistic activity of actinobacteria from forage soils in the flooding pampa, argentina. a total of 32 saprophytic strains of actinobacteria were obtained by different isolation methods from rhizospheric soil of lotus tenuis growing in the salado river basin. based on physiological trait ... | 2016 | 27550574 |
| the absence of protein y4ys affects negatively the abundance of t3ss mesorhizobium loti secretin, rhcc2, in bacterial membranes. | mesorhizobium loti maff303099 has a functional type iii secretion system (t3ss) that is involved in the determination of nodulation competitiveness on lotus. the m. loti t3ss cluster contains gene y4ys (mlr8765) that codes for a protein of unknown function (y4ys). a mutation in the y4ys gene favors the m. loti symbiotic competitive ability on lotus tenuis cv. esmeralda and affects negatively the secretion of proteins through t3ss. here we localize y4ys in the bacterial membrane using a translati ... | 2015 | 25688250 |
| lotus tenuis tolerates combined salinity and waterlogging: maintaining o2 transport to roots and expression of an nhx1-like gene contribute to regulation of na+ transport. | salinity and waterlogging interact to reduce growth for most crop and pasture species. the combination of these stresses often cause a large increase in the rate of na(+) and cl(-) transport to shoots; however, the mechanisms responsible for this are largely unknown. to identify mechanisms contributing to the adverse interaction between salinity and waterlogging, we compared two lotus species with contrasting tolerances when grown under saline (200 mm nacl) and o(2)-deficient (stagnant) treatmen ... | 2010 | 20444189 |
| characterization of strains unlike mesorhizobium loti that nodulate lotus spp. in saline soils of granada, spain. | lotus species are forage legumes with potential as pastures in low-fertility and environmentally constrained soils, owing to their high persistence and yield under those conditions. the aim of this work was the characterization of phenetic and genetic diversity of salt-tolerant bacteria able to establish efficient symbiosis with lotus spp. a total of 180 isolates able to nodulate lotus corniculatus and lotus tenuis from two locations in granada, spain, were characterized. molecular identificatio ... | 2010 | 20435777 |
| ensifer meliloti bv. lancerottense establishes nitrogen-fixing symbiosis with lotus endemic to the canary islands and shows distinctive symbiotic genotypes and host range. | eleven strains were isolated from root nodules of lotus endemic to the canary islands and they belonged to the genus ensifer, a genus never previously described as a symbiont of lotus. according to their 16s rrna and atpd gene sequences, two isolates represented minority genotypes that could belong to previously undescribed ensifer species, but most of the isolates were classified within the species ensifer meliloti. these isolates nodulated lotus lancerottensis, lotus corniculatus and lotus jap ... | 2009 | 19477097 |
| nodule development induced by mesorhizobium loti mutant strains affected in polysaccharide synthesis. | the role of mesorhizobium loti surface polysaccharides on the nodulation process is not yet fully understood. in this article, we describe the nodulation phenotype of mutants affected in the synthesis of lipopolysaccharide (lps) and beta(1,2) cyclic glucan. m. loti lpsbeta2 mutant produces lps with reduced amount of o-antigen, whereas m. loti lpsbeta1 mutant produces lps totally devoid of o-antigen. both genes are clustered in the chromosome. based on amino acid sequence homology, lps sugar comp ... | 2005 | 15915643 |