Publications
| a physical map of the mycoplasma agalactiae strain pg2 genome. | we have constructed a physical map of the mycoplasma agalactiae strain pg2 chromosome analyzing it by pulsed field gel electrophoresis in a contour-clamped homogeneous electric-field system. we mapped 33 cleavage sites generated with smai, xhoi, sali, eclxi and bsiwi restriction endonucleases using double digestions, one- and two-dimensional pulsed electrophoresis, cross-hybridization and linking clones. we have also mapped the loci of some genes by southern hybridization. | 2001 | 11295332 |
| development of a real-time pcr for detection of mycoplasma bovis in bovine milk and lung samples. | a real-time polymerase chain reaction (pcr) assay using hybridization probes on a lightcycler platform was developed for detection of mycoplasma bovis from individual bovine mastitis milk and pneumonic lung tissues. the detection limit was 550 colony forming units (cfu)/ml of milk and 650 cfu/25 mg of lung tissue. a panel of bovine mycoplasma and of other bovine-origin bacteria were tested; only m. bovis strains were positive, with a melting peak of 66.6 degrees c. mycoplasma agalactiae pg2 was ... | 2005 | 16475511 |