| age-dependent wound induction of a myrosinase-associated protein from oilseed rape (brassica napus). | in order to study the expression of the induced form of myrosinase-associated protein (imyap), a genomic clone encoding the protein was isolated from brassica napus. the coding portion of the gene was found to consist of five exons separated by one long intron of 938 bp and three shorter introns of ca. 100 bp. a 1.9 kb promoter fragment including the 5'-untranslated region was cloned in front of the coding portion of the escherichia coli iuda gene and transformed into arabidopsis thaliana. expre ... | 1999 | 10579485 |
| complex formation of myrosinase isoenzymes in oilseed rape seeds are dependent on the presence of myrosinase-binding proteins. | the enzyme myrosinase (ec 3.2.3.1) degrades the secondary compounds glucosinolates upon wounding and serves as a defense to generalist pests in capparales. certain myrosinases are present in complexes together with other proteins such as myrosinase-binding proteins (mbp) in extracts of oilseed rape (brassica napus) seeds. immunhistochemical analysis of wild-type seeds showed that mbps were present in most cells but not in the myrosin cells, indicating that the complex formation observed in extra ... | 2002 | 12177471 |
| unikaryon phyllotretae sp. n. (protista, microspora), a new microsporidian pathogen of phyllotreta undulata (coleoptera; chrysomelidae). | the microsporidium unikaryon phyllotretae sp. n., a new pathogen of phyllotreta undulata, is described based on light microscopic and ultrastructural characteristics. microscopic examination of parasitized individuals revealed two types of spores. the majority of the spores were of the first type, which are oval and measured 2.74+/-0.17 x 1.93+/-0.17 microm when fresh. fresh spores of the second type (very rare) are elongated and measured 4.39+/-0.18 x 1.61+/-0.20 microm. all life stages have si ... | 2010 | 19767185 |