| paenibacillus yonginensis sp. nov., a potential plant growth promoting bacterium isolated from humus soil of yongin forest. | strain dcy84(t), a gram-stain positive, rod-shaped, aerobic, spore-forming bacterium, motile by means of peritrichous flagella, was isolated from humus soil from yongin forest in gyeonggi province, south korea. strain dcy84(t) shared the highest sequence similarity with paenibacillus barengoltzii kacc 15270(t) (96.86 %), followed by paenibacillus timonensis kacc 11491(t) (96.49 %) and paenibacillus phoenicis nbrc 106274(t) (95.77 %). strain dcy84(t) was found to able to grow best in tsa at tempe ... | 2014 | 25169799 |
| ammoniibacillus agariperforans gen. nov., sp. nov., a thermophilic, agar-degrading bacterium isolated from compost. | a thermophilic, agar-degrading bacterium, strain fab2(t), was isolated from sewage sludge compost. according to phylogenetic analysis based on 16s rrna gene sequences, strain fab2(t) belonged to the family paenibacillaceae within the phylum firmicutes. however, fab2(t) was different enough at the genus level from closely related species. the percentages of 16s rrna gene sequence similarity with related organisms were 90.4 % for thermobacillus xylanilyticus, 91.8 % for paenibacillus barengoltzii, ... | 2015 | 25404482 |
| paenibacillus faecis sp. nov., isolated from human faeces. | a spore-forming, rod-shaped gram-strain-positive bacterium, strain 656.84t, was isolated from human faeces in 1984. it contained anteiso-c15 : 0 as the major cellular fatty acid, meso-diaminopimelic acid was found in the cell wall peptidoglycan, the polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and aminophospholipids as the major components, and the predominant menaquinone was mk-7. the dna g+c content was 52.9 mol%. the results of compar ... | 2015 | 26394885 |
| paenibacillus cathormii sp. nov., isolated from tree bark. | a gram-staining-positive, facultatively anaerobic, rod-shaped and endospore-forming bacterium, strain bk114-2t isolated from tree bark in thailand was characterized taxonomically using a polyphasic approach. analysis based on the 16s rrna gene sequences comparison indicated that strain bk114-2t was affiliated to the genus paenibacillus, and was closely related to paenibacillus timonensis 2301032t (96.7 %), paenibacillus phoenicis 3po2sat (96.6 %) and paenibacillus barengoltzii safn-016t (96.4 %) ... | 2015 | 26675308 |
| paenibacillus cisolokensis sp. nov., isolated from litter of a geyser. | a gram-stain-positive, endospore-forming, aerobic and thermophilic bacterium, designated strain lc2-13at, was isolated from cisolok geyser, west java, indonesia, at 50 °c. the isolate was rod-shaped and motile by means of peritrichous flagella. the major cellular fatty acids were iso-c16 : 0, c16 : 0 and anteiso-c15 : 0 and the major quinone was menaquinone 7. the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. the genomic dna g+c content was 56 ... | 2016 | 27188601 |
| an acidic, thermostable exochitinase with β-n-acetylglucosaminidase activity from paenibacillus barengoltzii converting chitin to n-acetyl glucosamine. | n-acetyl-β-d-glucosamine (glcnac) is widely used as a valuable pharmacological agent and a functional food additive. the traditional chemical process for glcnac production has some problems such as high production cost, low yield, and acidic pollution. hence, to identify a novel chitinase that is suitable for bioconversion of chitin to glcnac is of great value. | 2014 | 25550712 |
| cloning, expression, purification and application of a novel chitinase from a thermophilic marine bacterium paenibacillus barengoltzii. | a novel chitinase gene (pbchi70) from a marine bacterium paenicibacillus barengoltzii was cloned and functionally expressed in escherichia coli. the recombinant enzyme (pbchi70) was purified to homogeneity with a recovery yield of 51.9%. the molecular mass of purified enzyme was estimated to be 70.0 kda by sds-page. pbchi70 displayed maximal activity at ph 5.5 and 55 °c, respectively. it exhibited strict substrate specificity for colloidal chitin, glycol chitin, powdery chitin, and n-acetyl chit ... | 2016 | 26304445 |
| gene cloning, functional expression and characterisation of a novel type i pullulanase from paenibacillus barengoltzii and its application in resistant starch production. | a novel pullulanase gene (pbpula) from paenibacillus barengoltzii was cloned. pbpula has an open reading frame of 2028 bp encoding 675 amino acids. it was heterologously expressed in escherichia coli as an intracellular soluble protein. the recombinant pullulanase (pbpula) was purified to homogeneity with a molecular mass of about 75 kda on sds-page. pbpula was optimally active at ph 5.5 and 50 °c. it was stable within ph 5.5-10.5. the enzyme exhibited strict substrate specificity towards pullul ... | 2016 | 26763762 |
| biochemical characterization of a novel l-asparaginase from paenibacillus barengoltzii being suitable for acrylamide reduction in potato chips and mooncakes. | a novel l-asparaginase gene (pbasnase) from paenibaeillus barengoltzii cau904 was cloned and expressed in escherichia coli. the l-asparaginase gene was 1011bp encoding 336 amino acids. multiple sequence alignment of pbasnase with other known l-asparaginases revealed that the enzyme showed high similarities with some rhizobial-type l-asparaginases, sharing the highest identity of 32% with a characterized l-asparaginase from rhizobium etli cfn 42, suggesting that it should be a novel l-asparaginas ... | 2017 | 27919811 |
| indigenous cellulolytic and hemicellulolytic bacteria enhanced rapid co-composting of lignocellulose oil palm empty fruit bunch with palm oil mill effluent anaerobic sludge. | the composting of lignocellulosic oil palm empty fruit bunch (opefb) with continuous addition of palm oil mill (pome) anaerobic sludge which contained nutrients and indigenous microbes was studied. in comparison to the conventional opefb composting which took 60-90 days, the rapid composting in this study can be completed in 40 days with final c/n ratio of 12.4 and nitrogen (2.5%), phosphorus (1.4%), and potassium (2.8%), respectively. twenty-seven cellulolytic bacterial strains of which 23 stra ... | 2013 | 24012093 |
| characterization of a microbial consortium capable of degrading lignocellulose. | a microbial consortium, designated wcs-6, was established by successive subcultivation in the presence of rice straw under static conditions. the degradation efficiencies of wsc-6 for 0.5g filter paper, cotton and rice straw after 3days of cultivation were 99.0-¦0.7%, 76.9-¦1.5% and 81.3-¦0.8%, respectively as determined by gravimetrical methods. nine bacterial isolates were obtained from wcs-6 plated under aerobic conditions, and sequencing of their 16s rdna indicated that these bacteria were r ... | 2011 | 21831630 |
| paenibacillus phoenicis sp. nov., isolated from the phoenix lander assembly facility and a subsurface molybdenum mine. | a novel gram-positive, motile, endospore-forming, aerobic bacterium was isolated from the nasa phoenix lander assembly clean room that exhibits 100 % 16s rrna gene sequence similarity to two strains isolated from a deep subsurface environment. all strains are rod-shaped, endospore-forming bacteria, whose endospores are resistant to uv radiation up to 500 j m(-2). a polyphasic taxonomic study including traditional phenotypic tests, fatty acid analysis, 16s rrna gene sequencing and dna-dna hybridi ... | 2010 | 20584815 |
| paenibacillus fonticola sp. nov., isolated from a warm spring. | a novel bacterial strain, designated zl(t), isolated from a warm spring in jhonglun, taiwan, was characterized by using a polyphasic taxonomic approach. the novel strain had chemotaxonomic and morphological properties consistent with its classification in the genus paenibacillus. cells were gram-variable, aerobic, sporulating, motile rods. 16s rrna gene sequence analysis demonstrated that this novel isolate was unique, showing 94.3 % sequence similarity to paenibacillus assamensis gptsa 11(t) an ... | 2007 | 17551056 |
| paenibacillus pasadenensis sp. nov. and paenibacillus barengoltzii sp. nov., isolated from a spacecraft assembly facility. | two novel spore-forming, gram-positive, mesophilic, heterotrophic bacteria representing two novel species were isolated from the jet propulsion laboratory spacecraft assembly facility (jpl-saf) at pasadena, ca, usa. the incidence of similar strains was examined by screening the growing collection of isolates ( approximately 400 strains) obtained from the jpl-saf using species-specific pcr primer sets designed from the 16s rrna gene sequences of strains safn-016(t) and safn-007(t). phylogenetic a ... | 2006 | 16825621 |