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additional records of metazoan parasites from caribbean marine mammals, including genetically identified anisakid nematodes.studies of marine mammal parasites in the caribbean are scarce. an assessment for marine mammal endo- and ectoparasites from puerto rico and the virgin islands, but extending to other areas of the caribbean, was conducted between 1989 and 1994. the present study complements the latter and enhances identification of anisakid nematodes using molecular markers. parasites were collected from 59 carcasses of stranded cetaceans and manatees from 1994 to 2006, including globicephala macrorhynchus, kogi ...200919582477
molecular characterization and phylogeny of anisakid nematodes from cetaceans from southeastern atlantic coasts of usa, gulf of mexico, and caribbean sea.in the present study, 407 anisakid nematodes, collected from 11 different species of cetaceans of the families delphinidae, kogiidae, physeteridae, and ziphiidae, from the southeastern atlantic coasts of usa, the gulf of mexico, and the caribbean sea, were examined morphologically and genetically characterized by pcr restriction fragment length polymorphism to identify them to species level, assess their relative frequencies in definitive hosts, and determine any host preference. sequence data f ...201121212982
morphological and genetic identification of anisakis paggiae (nematoda: anisakidae) in dwarf sperm whale kogia sima from brazilian waters.anisakid nematodes have been identified in a wide variety of fish and marine mammal species. in brazil, anisakis physeteris, a. insignis, a. typica, a. nascetti, and those of the a. simplex complex have been reported infecting fishes and cetaceans. in this study, specimens collected from a dwarf sperm whale kogia sima (owen, 1866) stranded on the northeastern coast of brazil were identified through morphological and genetic analyses as a. paggiae. anisakids were examined through differential int ...201525751853
occurrence and prevalence of fish-borne anisakis larvae in the spotted mackerel scomber australasicus from taiwanese waters.anisakid nematodes have been found in a variety of marine fishes throughout the world and they are known to cause anisakiasis in human hosts. the present study investigated the prevalence of potentially zoonotic anisakid larvae in spotted mackerel caught from taiwanese waters where fish represents an important food sources. anisakis third-stage larvae (l3, n=502) were isolated from 250 spotted mackerel scomber australasicus. anisakis l3 larvae were divided morphologically into two types, anisaki ...201525733490
morphological and molecular characterization of anisakis larvae (nematoda: anisakidae) in beryx splendens from japanese waters.the third-stage (l3) larvae of anisakis, which are the etiological agents of human anisakiasis, have been categorized morphologically into anisakis type i larvae and anisakis type ii larvae. genetic analysis has allowed easy identification of these larvae: anisakis type i larvae include the species anisakis simplex sensu stricto, anisakis pegreffii, anisakis simplex c, anisakis typica, anisakis ziphidarum, and anisakis nascettii, whereas anisakis type ii larvae include the species anisakis physe ...201121377540
integrative taxonomy of anisakid nematodes in stranded cetaceans from brazilian waters: an update on parasite's hosts and geographical records.anisakidae are represented mainly by species of anisakis dujardin, 1845, pseudoterranova krabbe, 1878, and contracaecum railliet and henry, 1913. integrative taxonomy, based on morphological and genetic approaches, can provide a precise identification of species, increasing the knowledge of their systematics, biology, and evolution. in this study, integrative taxonomy was applied in order to identify, at generic and/or specific taxonomic level, 245 anisakids from 63 cetaceans of 12 different spe ...201728975458
experimental demonstration of pathogenic potential of anisakis physeteris and anisakis paggiae in wistar rats.anisakis morphotype i is the principal etiologic agent of human anisakiasis, with differences in pathogenicity found between the anisakis simplex s.s. and a. pegreffii species; however, the role of morphotype ii larvae in this illness is not well understood. the purpose of this study is to verify the ability of morphotype ii larvae to invade tissues via the experimental infection of wistar rats, an animal model which simulates infection in humans. in the in vivo assay, 7.1% (4/56 l3 morphotype i ...201425240961
effect of different temperatures on the expression of the newly characterized heat shock protein 90 (hsp90) in l3 of anisakis spp. isolated from scomber australasicus.anisakid nematodes are distributed worldwide in a wide variety of marine fishes and they are known to cause the zoonotic disease, anisakiasis. the temperature control is commonly applied for prevention and control of anisakiasis. to analyze the cellular response to temperature stress in anisakis, the heat shock protein 90 (hsp90) was chosen in the present study, as it plays a key role in many cellular processes and responds to stress conditions such as heat or cold shock. anisakids were sampled ...201425269988
evidence for a new species of anisakis dujardin, 1845: morphological description and genetic relationships between congeners (nematoda: anisakidae).in the present study, a new biological species of anisakis dujardin, 1845, was detected in kogia breviceps and k. sima from west atlantic waters (coast of florida) on the basis of 19 (nuclear) structural genes studied by multilocus allozyme electrophoresis. fixed allele differences at 11 enzyme loci were found between specimens of both adults and larvae of the new species and the other anisakis spp. tested. reproductive isolation from a. brevispiculata dollfus, 1968 was demonstrated by the lack ...200516025205
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