| peptostreptococcus stomatis sp. nov., isolated from the human oral cavity. | seven strains of anaerobic gram-positive cocci isolated from human oral sites were subjected to a comprehensive range of phenotypic and genotypic tests. 16s rrna gene sequence analysis revealed that the strains constituted a homogeneous group that was distinct from species with validly published names, but related to peptostreptococcus anaerobius. all oral strains tested belonged to this group, whereas all non-oral strains studied were confirmed as p. anaerobius. a novel species, peptostreptococ ... | 2006 | 16585688 |
| antimicrobial susceptibilities of peptostreptococcus anaerobius and the newly described peptostreptococcus stomatis isolated from various human sources. | peptostreptococcus anaerobius sensu lato, currently including two closely related species, p. anaerobius and p. stomatis, is known to be more resistant than other gram-positive anaerobic cocci. we reidentified potential peptostreptococcus isolates and tested their susceptibilities to eight antimicrobials. notably, p. anaerobius had constantly higher values for the mic at which 50% of the isolates are inhibited (mic(50)) and the mic(90) than p. stomatis. | 2007 | 17403999 |
| prospective study of bacteremia and complications with eus fna of rectal and perirectal lesions. | recent studies showed that endoscopic ultrasound (eus)-guided fine-needle aspiration (fna) is a low-risk procedure for causing bacteremia and infectious complications when sampling solid lesions of the upper gastrointestinal (gi) tract. as a result, antibiotics are not recommended for prophylaxis against endocarditis. our aim was to prospectively evaluate the risk of bacteremia and other infectious complications in patients undergoing eus fna of lower gi tract lesions. | 2007 | 17544995 |
| development of 16s rrna-based probes for the identification of gram-positive anaerobic cocci isolated from human clinical specimens. | fluorescent probes targeted at 16s rrna were designed for peptostreptococcus anaerobius and peptostreptococcus stomatis (pana134), parvimonas micra (pamic1435), finegoldia magna (fmag1250), peptoniphilus asaccharolyticus (pnasa1254), peptoniphilus ivorii (pnivo731), peptoniphilus harei (pnhar1466), anaerococcus vaginalis (avag1280) and anaerococcus lactolyticus (alac1438), based on the 16s rrna sequences of reference strains and 88 randomly chosen clinical isolates. these strains were also used ... | 2007 | 17714522 |
| peptostreptococcus russellii sp. nov., isolated from a swine-manure storage pit. | a polyphasic taxonomic study was performed on seven strains of an unknown gram-positive, nonspore-forming, obligately anaerobic coccus-shaped bacterium isolated from a swine-manure storage pit. comparative 16s rrna gene sequencing confirmed that all these isolates were highly related to each other and formed a hitherto unknown linage within the clostridial rrna xi cluster of organisms. pairwise analysis demonstrated that the novel organism to be most closely related to peptostreptococcus anaerob ... | 2010 | 20833884 |
| analysis of microbiota associated with peri-implantitis using 16s rrna gene clone library. | peri-implantitis (pi) is an inflammatory disease which leads to the destruction of soft and hard tissues around osseointegrated implants. the subgingival microbiota appears to be responsible for peri-implant lesions and although the complexity of the microbiota has been reported in pi, the microbiota responsible for pi has not been identified. | 2010 | 21523229 |
| development and pyrosequencing analysis of an in-vitro oral biofilm model. | dental caries and periodontal disease are the commonest bacterial diseases of man and can result in tooth loss. the principal method of prevention is the mechanical removal of dental plaque augmented by active agents incorporated into toothpastes and mouthrinses. in-vitro assays that include complex oral bacterial biofilms are required to accurately predict the efficacy of novel active agents in vivo. the aim of this study was to develop an oral biofilm model using the calgary biofilm device (cb ... | 2015 | 25880819 |
| tongue coating and the salivary microbial communities vary in children with halitosis. | halitosis is a common symptom mainly caused by microbial activities in the oral cavity. here, we used 16s rrna gene pyrosequencing and metagenomic sequencing to examine oral microbial compositions and their functional variations in children with halitosis. we found that the tongue coating of subjects with halitosis had greater bacterial richness than those of healthy subjects. the relative abundance and prevalence of leptotrichia wadei and peptostreptococcus stomatis were higher in tongue coatin ... | 2016 | 27080513 |
| comparison of oral microbiota in tumor and non-tumor tissues of patients with oral squamous cell carcinoma. | bacterial infections have been linked to malignancies due to their ability to induce chronic inflammation. we investigated the association of oral bacteria in oral squamous cell carcinoma (oscc/tumor) tissues and compared with adjacent non-tumor mucosa sampled 5 cm distant from the same patient (n = 10). by using culture-independent 16s rrna approaches, denaturing gradient gel electrophoresis (dgge) and cloning and sequencing, we assessed the total bacterial diversity in these clinical samples. | 2012 | 22817758 |
| bacterial diversity of symptomatic primary endodontic infection by clonal analysis. | the aim of this study was to explore the bacterial diversity of 10 root canals with acute apical abscess using clonal analysis. samples were collected from 10 patients and submitted to bacterial dna isolation, 16s rrna gene amplification, cloning, and sequencing. a bacterial genomic library was constructed and bacterial diversity was estimated. the mean number of taxa per canal was 15, ranging from 11 to 21. a total of 689 clones were analyzed and 76 phylotypes identified, of which 47 (61.84%) w ... | 2016 | 27737357 |
| molecular identification of cultivable bacteria from infected root canals associated with acute apical abscess. | the objective of this study was to investigate the bacterial composition present in root canals of teeth associated with acute apical abscess by molecular identification (16s rrna) of cultivable bacteria. two hundred and twenty strains isolated by culture from 20 root canals were subjected to dna extraction and amplification of the 16s rrna gene (pcr), followed by sequencing. the resulting nucleotide sequences were compared to the genbank database from the national center of biotechnology inform ... | 2017 | 27224567 |
| microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation. | this study was conducted to evaluate the microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation (cmp). | 2015 | 26521147 |
| microbiota-based model improves the sensitivity of fecal immunochemical test for detecting colonic lesions. | colorectal cancer (crc) is the second leading cause of death among cancers in the united states. although individuals diagnosed early have a greater than 90% chance of survival, more than one-third of individuals do not adhere to screening recommendations partly because the standard diagnostics, colonoscopy and sigmoidoscopy, are expensive and invasive. thus, there is a great need to improve the sensitivity of non-invasive tests to detect early stage cancers and adenomas. numerous studies have i ... | 2016 | 27056827 |
| metagenomic analysis of faecal microbiome as a tool towards targeted non-invasive biomarkers for colorectal cancer. | to evaluate the potential for diagnosing colorectal cancer (crc) from faecal metagenomes. | 2017 | 26408641 |