| sanitary status of oocytes and embryos collected from heifers experimentally exposed to leptospira borgpetersenii serovar hardjobovis. | in a preliminary trial and three experiments, a total of 30 holstein heifers were experimentally infected with a culture of leptospira borgpetersenii serovar hardjobovis via one or more routes (uterine, cervical supraconjunctival, intranasal) and oviductal and uterine fluids recovered post-mortem or in vivo following superovulation with fsh. all routes of administration were effective in establishing leptospira infection in the reproductive tract and leptospira were identified in the oviductal a ... | 1998 | 9877053 |
| genetic organization of the lipopolysaccharide o-antigen biosynthetic locus of leptospira borgpetersenii serovar hardjobovis. | leptospiral lps plays a critical role in immunity to leptospirosis and forms the basis for serological classification of leptospira. however, neither the structure of leptospiral lps nor the genetics of its biosynthesis have been elucidated. a probe derived from the rhamnose biosynthetic genes of l. interrogans serovar copenhageni was used to identify the rfb locus of l. borgpetersenii serovar hardjobovis. chromosome walking and sequence analysis revealed an rfb locus spanning 36.7 kb, which con ... | 1999 | 10458921 |
| pcr genome walking identifies a genetic locus comprising two heat shock genes (hslv and hslu) from leptospira borgpetersenii serovar hardjobovis. | pcr walking on genomic dna of leptospira borgpetersenii serovar hardjobovis has identified a genetic locus comprising two heat shock genes (hslv and hslu). this is the first molecular study on hslv and hslu in a leptospira species. the hslv gene has an open reading frame (orf) of 543 bp coding for a 180-amino acid polypeptide (19.476 kd) with 49-62% identity to other bacterial hslv homologs. forty-three nucleotides downstream from the hslv stop codon was found a second heat shock gene hslu with ... | 2001 | 11685516 |
| comparison of gene expression by co-cultured wc1+ gammadelta and cd4+ alphabeta t cells exhibiting a recall response to bacterial antigen. | immunization of cattle with a leptospira borgpetersenii serovar hardjo-bovis vaccine results in the development of a recall response by wc1(+) gammadelta t cells and cd4(+) alphabeta t cells characterized by proliferation and interferon-gamma production. it was hypothesized that these two t cell subpopulations had largely redundant effector functions, principally differing in their requirements for activation. to test this, gene expression in cells proliferating to antigen were compared utilizin ... | 2007 | 17081609 |
| prevalence of pathogenic leptospira spp. in sheep in a sheep-only abattoir in new zealand. | to determine the prevalence of the two most commonly diagnosed pathogenic leptospira spp. serovars, hardjobovis and pomona, in sheep in a sheep-only abattoir in new zealand, and to determine the prevalence of kidneys which were leptospire culture-positive collected from sheep seropositive or seronegative to the microscopic agglutination test (mat). | 2008 | 18690252 |
| are white-spot lesions in kidneys in sheep associated with leptospirosis? | to determine the association between white-spot lesions in kidneys and serological and cultural prevalence of leptospirosis in sheep, and to evaluate the diagnostic value of these lesions in individual sheep and lines of sheep at slaughter as indicators of past or current episodes of leptospirosis. | 2009 | 19252540 |
| comparison of fluorescent antibody and microscopic agglutination testing for leptospira in pregnant and nonpregnant cows. | serum and urine samples from 30 cows (15 pregnant and 15 nonpregnant) from each of 10 georgia dairy herds (total cows = 300) were examined by microscopic agglutination testing (mat) and direct fluorescent antibody testing (fat), respectively. seven of the 10 herds had at least 1 cow with a positive fat, and all of the herds had at least 1 cow with a reciprocal mat titer > or =100 for 1 or more serovars. serological testing was not helpful in identifying the infecting serovar for cows with a posi ... | 2010 | 20093682 |
| investigation of localisation of leptospira spp. in uterine and fetal tissues of non-pregnant and pregnant farmed deer. | to find evidence for localisation in the uterus, and fetal infection, of leptospira spp. in farmed deer in the lower north island of new zealand during and shortly after the breeding season. | 2010 | 21151213 |
| serosurvey of leptospirosis and investigation of a possible novel serovar arborea in farmed deer in new zealand. | to investigate the prevalence of leptospira spp. and possible novel serovar arborea infection in farmed deer in new zealand. | 2011 | 21541888 |
| growth response and shedding of leptospira spp. in urine following vaccination for leptospirosis in young farmed deer. | abstract | 2012 | 22175424 |