| mapping of betapapillomavirus human papillomavirus 5 transcription and characterization of viral-genome replication function. | betapapillomavirus replication and transcription have not been studied in detail because of a lack of suitable cellular systems supporting human papillomavirus (hpv) genome replication. we have recently shown that the human osteosarcoma cell line u2os provides a useful environment for the genome replication of many different hpvs, including the betapapillomaviruses hpv5 and hpv8. using mutational analysis and complementation assay, we demonstrated herein that the viral early proteins e1 and e2 a ... | 2014 | 24198410 |
| human papillomavirus 5 and 8 e6 downregulate interleukin-8 secretion in primary human keratinocytes. | human papillomaviruses (hpvs) of the genus betapapillomavirus appear to be involved in the early stages of skin cancer development, since both the prevalence and viral load are higher in precancerous actinic keratoses than in skin cancers. interleukin-8 (il-8) is an inflammatory cytokine that serves to alert the surrounding tissue after uv-induced damage. we examined the effects of the e2, e6 and e7 proteins of hpv8 and the e6 proteins of various hpv genotypes on il-8 secretion from primary kera ... | 2010 | 20007354 |
| uv-b irradiation stimulates the promoter activity of the high-risk, cutaneous human papillomavirus 5 and 8 in primary keratinocytes. | human papillomaviruses (hpv) have been implicated in the development of non-melanoma skin cancer (nmsc). hpv types 5 and 8 are strongly associated with nmsc in patients with the inherited disease epidermodysplasia verruciformis (ev). in these patients tumours arise predominantly on sun-exposed skin and consistently harbour hpv dnas. to determine whether uv-b irradiation modulates the noncoding region (ncr) promoter activity of the ev-hpv types 5, 8, 9, 14, 23, 24, and 25 we performed transient t ... | 2004 | 15654507 |
| a high association of oncogenic human papillomaviruses with carcinomas of the female urethra: polymerase chain reaction-based analysis of multiple histological types. | using polymerase chain reaction with type-specific human papillomavirus type 16 and 18 primers, and general primers screening for 9 other genotypes, we analyzed archival surgical specimens of urethral carcinoma from 18 women (17 with invasive cancer and 1 with carcinoma in situ). human papillomavirus was detected in invasive urethral carcinoma specimens from 10 of 17 women (59%) and in the patient with carcinoma in situ. human papillomavirus type 16 was found in 8 patients with invasive carcinom ... | 1994 | 8254831 |
| human papilloma virus 5-dna in a carcinoma of an epidermodysplasia verruciformis patient infected with various human papillomavirus types. | an epidermodysplasia verruciformis patient suffering from generalized warts and a carcinoma at the forehead was found to be infected by at least six types and subtypes of human papillomaviruses. the central part of the carcinoma, however, harbored only human papillomavirus 5 dna. the dna persisted extrachromosomally in high genome copy number. in contrast to wart dna preparations, a significant part of the viral sequences in the carcinoma was present as oligomers, at least part of them being con ... | 1983 | 6297727 |
| a subtype of human papillomavirus 5 (hpv-5b) and its subgenomic segment amplified in a carcinoma: nucleotide sequences and genomic organizations. | a subtype of human papillomavirus 5 (hpv-5b) is closely associated with carcinomas in the disease epidermodysplasia verruciformis (ev). the complete genome was cloned from virus particles in benign lesions of a patient with ev and sequenced: it was 7779 nucleotides long and consisted of six open reading frames (orfs) (e6, e7, e1, e2, e4, and e5) in the early region, three orfs (l2, l3, and l1) in the late region, and a noncoding region, all existing on one dna strand. the 40% segment of the hpv- ... | 1991 | 1649510 |
| possible involvement of epidermodysplasia verruciformis human papillomaviruses in the immunopathogenesis of psoriasis: a proposed hypothesis. | we have shown previously in psoriasis a very high prevalence of epidermodysplasia verruciforms-associated human papillomavirus 5 (evhpv5) dna and antibodies to human papillomavirus 5 (hpv5) virus-like particle (vlp) l1, and we suggested that this benign hyperproliferative disorder could be a reservoir for evhpvs. here we provide new data confirming the expression of evhpvs in psoriasis and present our hypothesis on their possible involvement in the immunopathogenesis of the disorder. the new imp ... | 2003 | 14714550 |