| human papillomavirus 16 and 18 dna can solely induce oncogenic transformation of mammalian cells in primary culture. | to compare the tumorigenic activity of hpv 6, 11, 16, and 18 dna in mammalian cells in primary culture, hpv dnas were transfected to syrian hamster embryo (she) cells. morphologically transformed foci were observed in all the hpv dna-transfected cultures and cells propagated from these foci were immortalized. all these cell lines at early passages were not tumorigenic in nude mice. however, a cell line derived from hpv 16 transfection became tumorigenic after 25 passages and a cell line derived ... | 1993 | 8383414 |
| the p53/retinoblastoma-mediated repression of testicular orphan receptor-2 in the rhesus monkey with cryptorchidism. | whereas the linkage of infertility to cryptorchidism, the failure of the testis to descend into the scrotum at birth, has been well documented, the detailed molecular mechanism remains unclear. here we report that the testicular orphan receptor-2 (tr2) expression, which modulates many signal pathways, was completely repressed in the surgery-induced cryptorchidism of the rhesus monkey. further studies link tr2 repression to the induction of p53 and results suggest that induced p53 could repress t ... | 2000 | 10811662 |
| [preparation and activity detection of chicken egg yolk igy antibody against human papillomavirus 16 type l1 main capsid protein]. | to prepare highly specific chicken egg yolk igy antibody against human papillomavirus 16 type l1 main capsid protein (hpv16l1) for detection of hpv16l1. | 2008 | 18359682 |
| effect of pap smear collection and carrageenan on cervicovaginal human papillomavirus-16 infection in a rhesus macaque model. | background human papillomavirus (hpv) infection of the genital mucosa is thought to require trauma to the cervicovaginal epithelium. therefore, we determined whether a cytology specimen collection procedure (pap smear), which disrupts the epithelium by design, renders the cervix more susceptible to hpv infection in a primate model. methods in a series of female rhesus macaques, a speculum examination was performed with (n = 8) or without (n = 4) a cytology specimen collection procedure as it is ... | 2011 | 21483020 |
| tumourigenesis driven by the human papillomavirus type 16 asian-american e6 variant in a three-dimensional keratinocyte model. | infection with a transforming human papillomavirus (hpv) such as type 16 (of species alphapapillomavirus 9) causes ano-genital and oral tumours via viral persistence in human squamous cell epithelia. epidemiological studies showed that the naturally occurring hpv16 asian-american (aa) variant (sublineage d2/d3) is found more often than the european prototype (ep) (sublineage a1) in high-grade cervical neoplasia and tumours compared to non-cancer controls. just three amino acid changes within the ... | 2014 | 24983759 |
| immune responses in macaques to a prototype recombinant adenovirus live oral human papillomavirus 16 vaccine. | immunization with human papillomavirus (hpv) l1 virus-like particles (vlps) prevents infection with hpv. however, the expense and logistical demands of current vlp vaccines will limit their widespread use in resource-limited settings, where most hpv-induced cervical cancer occurs. live oral adenovirus vaccines have properties that are well-suited for use in such settings. we have described a live recombinant adenovirus vaccine prototype that produces abundant hpv16 l1 protein from the adenovirus ... | 2014 | 24990902 |
| characterization of mus musculus papillomavirus 1 infection in situ reveals an unusual pattern of late gene expression and capsid protein localization. | full-length genomic dna of the recently identified laboratory mouse papillomavirus 1 (muspv1) was synthesized in vitro and was used to establish and characterize a mouse model of papillomavirus pathobiology. muspv1 dna, whether naked or encapsidated by muspv1 or human papillomavirus 16 (hpv 16) capsids, efficiently induced the outgrowth of papillomas as early as 3 weeks after application to abraded skin on the muzzles and tails of athymic ncr nude mice. high concentrations of virions were extrac ... | 2013 | 24067981 |
| spontaneous and vaccine-induced clearance of mus musculus papillomavirus 1 infection. | mus musculus papillomavirus 1 (mmupv1/muspv1) induces persistent papillomas in immunodeficient mice but not in common laboratory strains. to facilitate the study of immune control, we sought an outbred and immunocompetent laboratory mouse strain in which persistent papillomas could be established. we found that challenge of skh1 mice (crl:skh1-hrhr) with mmupv1 by scarification on their tail resulted in three clinical outcomes: (i) persistent (>2-month) papillomas (∼20%); (ii) transient papillom ... | 2017 | 28515303 |
| long-term toxicity, pharmacokinetics and immune effects of a recombinant adenovirus vaccine expressing human papillomavirus 16 e6 and e7 proteins (hpv16 e6e7-ad5 vac) in primates. | this study aimed to: evaluate long-term toxicity and pharmacokinetic parameters; to identify the target organ of toxicity of a recombinant adenovirus vaccine expressing human papillomavirus 16 e6 and e7 proteins (hpv16 e6e7-ad5 vac) in primates; and to determine the specific immune response of this recombinant adenovirus vaccine. | 2018 | 29887967 |