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antimicrobial activity and identification of potential antimicrobial compounds from aquatic pteridophyte, azolla microphylla kaulf.azolla microphylla kaulf. is an aquatic nitrogen fixing pteridophyte commonly found in aquatic habitats including paddy fields. methanolic extract of the fronds of a. microphylla was subjected to partial purification by solvent partitioning with diethyl ether and ethyl acetate followed by hydrolysis, and further partitioning with ethyl acetate. the two fractions, thus obtained were tested for antibacterial activity. it was observed that the ethyl acetate fraction inhibited the growth of the path ...201526011985
induction of salt tolerance in azolla microphylla kaulf through modulation of antioxidant enzymes and ion transport.azolla microphylla plants exposed directly to nacl (13 dsm(-1)) did not survive the salinity treatment beyond a period of one day, whereas plants exposed directly to 4 and 9 dsm(-1) nacl were able to grow and produce biomass. however, plants pre-exposed to nacl (2 dsm(-1)) for 7 days on subsequent exposure to 13 dsm(-1) nacl were able to grow and produce biomass although at a slow rate and are hereinafter designated as pre-exposed plants. the pre-exposed and directly exposed plants distinctly di ...201020422236
cellular responses in the cyanobacterial symbiont during its vertical transfer between plant generations in the azolla microphylla symbiosis.the nitrogen-fixing symbiosis between cyanobacteria and the water fern azolla microphylla is, in contrast to other cyanobacteria-plant symbioses, the only one of a perpetual nature. the cyanobacterium is vertically transmitted between the plant generations, via vegetative fragmentation of the host or sexually within megasporocarps. in the latter process, subsets of the cyanobacterial population living endophytically in the azolla leaves function as inocula for the new plant generations. using el ...200919076717
protective effect of azolla microphylla on biochemical, histopathological and molecular changes induced by isoproterenol in rats.azolla microphylla is an important fast-growing aquatic plant trusted for its agronomic, nutritious and therapeutic uses. the present work is undertaken to investigate the protective effect of the ethanolic extract of azolla microphylla (eam) against the isoproterenol (iso) induced cardiotoxicity in rats. rats were pre-treated with eam (250 and 500mg/kg b.w.) for 28 days along with iso (85mg/kg; s.c.) on the 29th and 30th days. iso-induced rats displayed significant diminution in cardiac antioxi ...201728249249
methane production in rice soil is inhibited by cyanobacteria.the present study was aimed at understanding the role of cyanobacteria and azolla in methane production and oxidation in laboratory simulation experiments using soil samples from rice fields. all the seven cyanobacterial strains tested effected a significant decrease in the headspace concentration of methane in flooded soil, incubated under light. synechocystis sp. was the most effective in retarding methane concentration by 10-20 fold over that in controls without cyanobacteria. the decrease in ...200211911608
evaluation of the water fern azolla microphylla for mosquito population management in the rice-land agro-ecosystem of south india.the floating water fern azolla microphylla kaulfess was evaluated as a biocontrol agent against mosquitoes breeding in rice fields in tamil nadu, south india. anopheles subpictus grassi, culex pseudovishnui colless and culex tritaeniorhynchus giles were the predominant species of mosquitoes, with peak densities of late instar larvae and pupae occurring during the second week after transplantation of rice seedlings of short-term (c. 80 days from transplantation to harvest) or medium-term (c. 95 d ...19911768923
salinity-induced inhibition of growth in the aquatic pteridophyte azolla microphylla primarily involves inhibition of photosynthetic components and signaling molecules as revealed by proteome analysis.salinity stress causes adverse physiological and biochemical changes in the growth and productivity of a plant. azolla, a symbiotic pteridophyte and potent candidate for biofertilizer due to its nitrogen fixation ability, shows reduced growth and nitrogen fixation during saline stress. to better understand regulatory components involved in salinity-induced physiological changes, in the present study, azolla microphylla plants were exposed to nacl (6.74 and 8.61 ds/m) and growth, photochemical re ...201726837223
in vitro antioxidant and hepatoprotective potential of azolla microphylla phytochemically synthesized gold nanoparticles on acetaminophen - induced hepatocyte damage in cyprinus carpio l.the present study aims to evaluate the hepatoprotective and antioxidant effects of gold nanoparticles (gnap) biosynthesized through the mediation of azolla microphylla and a. microphylla extract on acetaminophen-induced hepatocyte damage in common carp fish (cyprinus carpio l.). the gold nanoparticles (100, 150, 200 μg/ml) and a. microphylla extract powder (100, 200, 400 μg/ml) were added to the primary hepatocytes in different conditions: treatment i (before 12 mm acetaminophen), treatment ii ( ...201525862331
multiple modes of cell death discovered in a prokaryotic (cyanobacterial) endosymbiont.programmed cell death (pcd) is a genetically-based cell death mechanism with vital roles in eukaryotes. although there is limited consensus on similar death mode programs in prokaryotes, emerging evidence suggest that pcd events are operative. here we present cell death events in a cyanobacterium living endophytically in the fern azolla microphylla, suggestive of pcd. this symbiosis is characterized by some unique traits such as a synchronized development, a vertical transfer of the cyanobacteri ...201323822984
interactive effects of herbicide and enhanced uv-b on growth, oxidative damage and the ascorbate-glutathione cycle in two azolla species.a field experiment was conducted to investigate the impact of alone and combined exposures of herbicide pretilachlor (5, 10 and 20μgml(-1)) and enhanced uv-b radiation (uv-b1; ambient +2.2kjm(-2) day(-1) and uv-b2; ambient +4.4kjm(-2) day(-1)) on growth, oxidative stress and the ascorbate-glutathione (asa-gsh) cycle in two agronomically important azolla spp. viz., azolla microphylla and azolla pinnata. decreased relative growth rate (rgr) in both the species under tested stress could be linked t ...201627497078
salinity induced physiological and biochemical changes in the freshly separated cyanobionts of azolla microphylla and azolla caroliniana.freshly separated cyanobionts of azolla microphylla and azolla caroliniana plants exposed to salinity showed decline in the cellular constituents such as chlorophyll (23.1 and 38.9%) and protein (12.9 and 19.3%). however, an increase in the carotenoid and sugar content was observed. exposure to salinity stress reduced the heterocyst frequency (35.4 and 57.2%) and nitrogenase activity (37.7 and 46.3%) of the cyanobionts. increase in the activity of antioxidant enzymes such as super oxide dismutas ...201627135817
dna probes show genetic variation in cyanobacterial symbionts of the azolla fern and a closer relationship to free-living nostoc strains than to free-living anabaena strains.twenty-two isolates of anabaena azollae derived from seven azolla species from various geographic and ecological sources were characterized by dna-dna hybridization. cloned dna fragments derived from the genomic sequences of three different a. azollae isolates were used to detect restriction fragment length polymorphism among all symbiotic anabaenas. dna clones were radiolabeled and hybridized against southern blot transfers of genomic dnas of different isolates of a. azollae digested with restr ...199016348182
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