| comparison of perillus bioculatus and podisus maculiventris (hemiptera:pentatomidae) as potential control agents of the colorado potato beetle (coleoptera:chrysomelidae). | in laboratory and microplot consumption tests, spined soldier bug, podisus maculiventris (say), ate at least as many colorado potato beetle, leptinotarsa decemlineata (say), eggs and larvae as did the twospotted stink bug, perillus bioculatus (f.). p. maculiventris also remained in field microplots infected with colorado potato beetle egg masses for slightly longer than did p. bioculatus. the 2 predators were equally effective at consuming egg masses, reducing densities of beetle larvae, and pre ... | 1996 | 8913113 |
| occurrence of digestive cysteine proteases in perillus bioculatus, a natural predator of the colorado potato beetle. | oryzacystatins (ocs) are protease inhibitors (pis) that inhibit colorado potato beetle (cpb) digestive proteases, and transgenic potato plants containing these pis are currently under test. however, ocs could interfere with the digestive system of beneficial insects. protease activity and susceptibility to class-specific protease inhibitors were studied in protein extracts of perillus bioculatus, a stinkbug predator that has shown potential for biological control of the cpb. at physiological ph, ... | 1998 | 9787788 |
| life history and cost analysis for continuous rearing of perillus bioculatus (heteroptera: pentatomidae) on a zoophytogenous artificial diet. | the impact of a zoophytogenous, insect-free artificial diet on the developmental rate, life history parameters, and fertility was examined over 11 consecutive generations for domesticated perillus biocullatus (f.) (heteroptera: pentatomidae). this study showed that when fed an insect-free artificial diet during both the nymphal and adult stages, developmental times were prolonged, and the net reproductive rates (r0) and the intrinsic rates of increase (r(m)) were significantly lower than when fe ... | 2004 | 15279257 |
| morphological diversity of the labial sensilla of phytophagous and predatory pentatomidae (hemiptera: heteroptera), with reference to their possible functions. | sensory structure on the labial surface of five genera of pentatomidae (hemiptera: heteroptera) belonging to two subfamilies i.e. asopinae and pentatominae have been studied using scanning electron microscopy. three representatives of the subfamily pentatominae (phytophagous)-dolycoris indicus (stal), plautia crossota (dallas) and piezodorus hybneri (gmelin) and two of asopinae (predatory)-perillus bioculatus (fabricius) and eocanthecona furcellata (wolff) were studied to morphologically charact ... | 2015 | 26624484 |
| oryzacystatin i expressed in transgenic potato induces digestive compensation in an insect natural predator via its herbivorous prey feeding on the plant. | we observed recently that the rice cysteine proteinase inhibitor, oryzacystatin i (oci) expressed in transgenic potato does not affect growth and development of the two-spotted stinkbug predator (perillus bioculatus) via its herbivorous prey feeding on the plant. here we monitored the inhibitory activity of recombinant oci along this potato --> herbivore --> predator continuum, to determine if the absence of effect was associated with a digestive compensatory response of the predator following i ... | 2003 | 12919481 |
| differential gene expression in perillus bioculatus nymphs fed a suboptimal artificial diet. | fragments of two artificial diet up-regulated and two prey up-regulated transcripts were isolated from the predatory pentatomid perillus bioculatus using suppression subtractive hybridization. a blastx search found similarities for two diet-upregulated clones, i.e., the tyrosine-3-monooxygenase gene and the gene for the chitin binding protein, gasp. the probe generated from the tyrosine-3-monooxygenase clone hybridized to two transcripts 2.3 and 1.2kb in size. the two transcripts were differenti ... | 2006 | 16580014 |
| resistant potato selections contain leptine and inhibit development of the colorado potato beetle (coleoptera: chrysomelidae). | we recently described a new source of host-plant resistance to the colorado potato beetle, leptinotarsa decemlineata (say), in a tetraploid potato (solanum tuberosum l.) selection, nd2858-1. this genotype, and selected backcross progeny, had little damage while check cultivars were defoliated in open-choice field assays. to further characterize the observed deterrence, we determined foliar glycoalkaloids and conducted no-choice assays with nd2858-1 backcross progeny genotypes (nd4382-n). develop ... | 2001 | 11681692 |
| tailoring the specificity of a plant cystatin toward herbivorous insect digestive cysteine proteases by single mutations at positively selected amino acid sites. | plant cystatins, similar to other defense proteins, include hypervariable, positively selected amino acid sites presumably impacting their biological activity. using 29 single mutants of the eighth domain of tomato (solanum lycopersicum) multicystatin, slcys8, we assessed here the potential of site-directed mutagenesis at positively selected amino acid sites to generate cystatin variants with improved inhibitory potency and specificity toward herbivorous insect digestive cysteine (cys) proteases ... | 2008 | 18192440 |
| precocious induction of vitellogenin with jh iii in the twospotted stink bug, perillus bioculatus (heteroptera: pentatomidae). | the effect of juvenile hormone (jh) iii on the hemolymph composition of vitellogenin was examined in perillus bioculatus. adult females were treated topically with jh iii, and the premature presence of vitellogenin in the hemolymph was then detected using electrophoresis and western blot analyses. jh iii treatment resulted in a dose-dependent early production of vitellogenin that was detectable 48 h before vitellogenin was present in non-treated insects. vitellogenin was not observed in the hemo ... | 2005 | 16129637 |
| partial morphological and functional characterization of the corpus allatum-corpus cardiacum complex from the two-spotted stinkbug, perillus bioculatus (hemiptera: pentatomidae). | selected morphological and physiological properties of the corpus allatum (ca)-corpus cardiacum (cc) complex from the two-spotted stinkbug, perillus bioculatus (hemiptera: pentatomidae), were studied. the cas play an important role in insect physiology because of their production of the juvenile hormones (jhs), i.e., key hormones involved in development and reproduction. we found that the p. bioculatus ca-cc complex is present in two distinct morphological forms, the more frequently observed com ... | 2005 | 16029075 |
| molecular interactions between an insect predator and its herbivore prey on transgenic potato expressing a cysteine proteinase inhibitor from rice. | transgenic plants expressing resistance to herbivorous insects may represent a safe and sustainable pest control alternative if they do not interfere with the natural enemies of target pests. here we examined interactions between oryzacystatin i (oci), a proteinase inhibitor from rice genetically engineered into potato (solanum tuberosum cv. kennebec, line k52) to increase resistance to insect herbivory, and the insect predator perillus bioculatus. this stinkbug is a relatively specialized preda ... | 2003 | 12919480 |
| effect of age, diet, diapause and juvenile hormone on oogenesis and the amount of vitellogenin and vitellin in the twospotted stink bug, perillus bioculatus (heteroptera: pentatomidae). | vitellogenic oocytes from perillus bioculatus have two native vitellins, vt1 and vt2, with molecular masses of 553 and 228 kda, respectively. the hemolymph contains a major vitellogenin, vg, with a molecular mass of 528 kda that consists of three apoproteins with masses of 177, 84 and 59 kda, respectively. antibodies to purified vt2 reacted with ovary extracts, egg extracts and female hemolymph, but not with male hemolymph in immunodiffusion tests. western blots showed that anti vt2 reacted with ... | 2002 | 12770097 |