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estimation and diversity of phylloplane mycobiota on selected plants in a mediterranean-type ecosystem in portugal.mediterranean ecosystems have not been consistently investigated as natural habitats for microbes in general, and fungi in particular. here we present the results of a survey of epiphytic mycobiota (filamentous fungi and yeasts) on the phylloplane of selected plants in the arrábida natural park, an ecosystem of mediterranean characteristics in portugal, using conventional culture-dependent isolation methods. leaves from the species acer monspessulanum and quercus faginea (deciduous trees) and ci ...200212375096
establishment of two ectomycorrhizal shrub species in a semiarid site after in situ amendment with sugar beet, rock phosphate, and aspergillus niger.a field experiment was carried out to assess the effectiveness of the addition of sugar beet, rock phosphate, and aspergillus niger directly into the planting hole, and the mycorrhizal inoculation of seedlings with scleroderma verrucosum, for promotion of plant growth of cistus albidus l. and quercus coccifera l. and enhancement of soil physicochemical, biochemical, and biological properties, in a degraded semiarid mediterranean area. one year after planting, the available phosphorus content in ...200515690228
effect of intraspecific competition and substrate type on terpene emissions from some mediterranean plant species.competition is an important factor that has been extensively reported in the mediterranean area. there is evidence that leaf terpene accumulation may vary between plants growing on calcareous and siliceous soils. in the present study, leaf terpene emissions from potted seedlings of pinus halepensis, cistus albidus, and quercus coccifera, growing under natural environmental conditions on calcareous and siliceous substrates, were studied by using a bag enclosure method. in both substrates, seedlin ...200717195116
production and diversity of volatile terpenes from plants on calcareous and siliceous soils: effect of soil nutrients.fertilizer effects on terpene production have been noted in numerous reports. in contrast, only a few studies have studied the response of leaf terpene content to naturally different soil fertility levels. terpene content, as determined by gas chromatography/mass spectrometry/flame ionization detector, and growth of pinus halepensis, rosmarinus officinalis, and cistus albidus were studied on calcareous and siliceous soils under field conditions. the effect of nitrogen (n) and extractable phospho ...200818670820
combined effects of clay immobilized azospirillum brasilense and pantoea dispersa and organic olive residue on plant performance and soil properties in the revegetation of a semiarid area.the reestablishment of autochthonous shrubs species is an essential strategy for recovering degraded soils under semiarid mediterranean areas. a field experiment was carried out to assess the effectiveness of an immobilized microbial inoculant (azospirillum brasilense and pantoea dispersa) and the addition of organic olive residue (alperujo), for plant growth promotion of cistus albidus l. and enhancement of soil properties. sixteen months after planting, the microbial inoculant and organic resi ...201423895777
whose truffle is this? distribution patterns of ectomycorrhizal fungal diversity in tuber melanosporum brûlés developed in multi-host mediterranean plant communities.in the mediterranean region, patches of vegetation recovering from disturbance and transiently dominated by shrubs produce one of the world's most prized fungi, the black truffle (tuber melanosporum). in these successional plant communities, we have fragmentary knowledge of the distribution of t. melanosporum in space among ectomycorrhizal (ecm) host species and in time. molecular identification of hosts (restriction fragment length polymorphism) and fungi (internal transcribed spacer sequencing ...201525522815
the mediterranean evergreen quercus ilex and the semi-deciduous cistus albidus differ in their leaf gas exchange regulation and acclimation to repeated drought and re-watering cycles.plants may exhibit some degree of acclimation after experiencing drought, but physiological adjustments to consecutive cycles of drought and re-watering (recovery) have scarcely been studied. the mediterranean evergreen holm oak (q. ilex) and the semi-deciduous rockrose (c. albidus) showed some degree of acclimation after the first of three drought cycles (s1, s2, and s3). for instance, during s2 and s3 both species retained higher relative leaf water contents than during s1, despite reaching si ...201121813795
assessment of phylloplane yeasts on selected mediterranean plants by fish with group- and species-specific oligonucleotide probes.a previous culture-dependent survey of phylloplane yeasts from selected mediterranean plants showed that a few species were present in high densities in almost all leaf samples, regardless of the plant type, location or sampling season. however, a few species appeared to be restricted to cistus albidus leaves, namely cryptococcus cistialbidi. here, we describe a culture-independent fish assay to detect and quantify whole yeast cells in leaf washings. after optimization, the technique was used to ...201019817863
plant coexistence alters terpene emission and content of mediterranean species.there is evidence that secondary metabolism may modulate plant interactions and is modified by different biotic stress agents, such as herbivores or pathogens. however, it is poorly understood whether secondary metabolism is altered during competition among plants. the intraspecific and interspecific coexistence of some mediterranean potted seedlings, namely rosmarinus officinalis, pinus halepensis, cistus albidus and quercus coccifera was investigated through their terpene accumulation within l ...200717258247
water deficit stress induces different monoterpene and sesquiterpene emission changes in mediterranean species. relationship between terpene emissions and plant water potential.the effects of water deficit stress and plant water potential (psi) on monoterpene and sesquiterpene leaf emissions from rosmarinus officinalis, pinus halepensis, cistus albidus and quercus coccifera were studied over 11 days of water withholding (from t(1) to t(11)), after substrates had achieved their field capacity (control pots: t(0)). volatile compounds were sampled from the same twig per plant all throughout the study, using a dynamic bag enclosure system. volatiles, collected in tenax ta, ...200717156816
seasonal patterns of non-terpenoid c6-c10 voc emission from seven mediterranean woody species.the seasonal pattern of non-terpenoid c6-c10 voc emission by seven mediterranean woody species (bupleurum fruticosum, cistus albidus, pinus halepensis, arbutus unedo, erica arborea, quercus coccifera, and q. ilex) was studied under field conditions. branch chamber samples were sorbed on carbotrap and analyzed by thermal desorption in combination with gc-ms. these non-terpenoid c6-c10 voc emissions were large, almost of similar magnitude to those of terpenes. overall, maximum values were recorded ...200111592412
seasonal patterns of terpene content and emission from seven mediterranean woody species in field conditions.the seasonal pattern of terpene content and emission by seven mediterranean woody species was studied under field conditions. emission rates were normalized at 30 degrees c and 1000 µmol.m.s pfd (photosynthetic photon flux density). bupleurum fruticosum, pinus halepensis, and cistus albidus stored large amounts of terpenes (0.01-1.77% [dry matter]) with maximum values in autumn and minimum values in spring. they emitted large amounts of terpenes (2-40 µg.g dm.h), but with no clear seasonal trend ...200010636836
[in process citation]this study focuses on how allelopathic mechanisms may be involved in the floristic composition and dynamics of cistus albidus shrubbery. allelopathic potential of essential oils was measured for lactuca sativa (toxicity for other species) and cistus albidus (self-toxicity). these oils have little effect on germination but significantly increase, even in low quantities, the number of unviable seedlings and inhibit normal seedling growth. these results explain, at least partially, the existence of ...199910505240
enhanced photo- and antioxidative protection, and hydrogen peroxide accumulation in drought-stressed cistus clusii and cistus albidus plants.mechanisms of drought stress resistance were studied in cistus clusii dunal and cistus albidus l., two native mediterranean shrubs that can withstand severe summer drought. while water deficit, solar radiation and temperature increased from winter to summer in the field, c. clusii and c. albidus reduced leaf area, increased root mass per leaf area, and showed diurnal changes in stomatal conductance to minimize water loss. in both species, the consequent reductions in co2 assimilation were accomp ...200312511299
is there a feedback between n availability in siliceous and calcareous soils and cistus albidus leaf chemical composition?the effects of the mediterranean shrub cistus albidus on n cycling were studied in two siliceous (granitic-derived and schistic-derived) and one calcareous soil differentiated by their texture and acidity. we aimed to find out whether soils under c. albidus were affected by the release of c compounds from the canopy, and whether phenolic compound production in c. albidus changed depending on the soil n availability. calcareous soils, with higher clay content and polyvalent cations, had a higher ...200312695905
prodelphinidin trimers and characterization of a proanthocyanidin oligomer from cistus albidus.two new natural prodelphinidin trimers have been isolated from the air-dried herb of cistus albidus, epigallocatechin-(4beta --> 8)-gallocatechin-(4alpha --> 8)-catechin and epigallocatechin-(4beta --> 8)-gallocatechin-(4alpha --> 8)-gallocatechin in addition to catechin, gallocatechin and thirteen known proanthocyanidins. the structures were determined on the basis of 1d- and reverse 2d-nmr (hsqc, hmbc) experiments of their peracetylated derivatives, maldi-tof-ms and by acid-catalysed degradati ...200312857007
differences in the effects of simulated sea aerosol on water relations, salt content, and leaf ultrastructure of rock-rose plants.white-leaf rock-rose (cistus albidus l.) and montpellier rock-rose (c. monspeliensis l.) plants were sprayed 2 to 3 min per day over a 7-d period, in an unheated plastic greenhouse, with different aqueous solutions containing deionized water alone (control); an anionic surfactant (sodium dodecylbenzenesulfonate 82.5%, 50 mg l(-1)) (s1); a solution simulating the composition of sea aerosol (s2); and a solution simulating sea aerosol with anionic surfactant (s3). white-leaf rock-rose was more sens ...201515254119
simulation of germination of pioneer species along an experimental drought gradient.the germination of ten plant species from the iberian peninsula was assessed along a water deficit gradient between -0. 1652 (moist) and -0.4988 mpa (dry) of osmotic potential, created by addition of increasing concentrations of polyethylene glycol (peg 6000) to distilled water in which plants were grown hydroponically. the level and rate of germination of daucus carota and thapsia villosa significantly decreased with decreasing psi. seeds of dactylis glomerata and dittrichia viscosa had positiv ...200617405330
detailed analysis of the essential oil from cistus albidus l. by combination of gc/ri, gc/ms and 13c-nmr spectroscopy.the composition of the essential oil of cistus albidus (l.) obtained from plants growing wild in provence (france) has been investigated using gc-ri (ri = retention indices), gc/ms and (13)c-nmr. eighty-eight components were reported accounting for 81.8% of the essential oil. this essential oil was characterized by a high content of sesquiterpenes with alpha-zingiberene (12.8%), alpha-curcumene (7.7%), (e)-beta-caryophyllene (5.9%), alpha-cadinol (5.4%), alpha-bisabolol (4.1%), caryophyllene oxi ...200818932091
hydrogen peroxide is involved in the acclimation of the mediterranean shrub, cistus albidus l., to summer drought.this study evaluated the possible role of hydrogen peroxide (h(2)o(2)) in the acclimation of a mediterranean shrub, cistus albidus l., to summer drought growing under mediterranean field conditions. for this purpose, changes in h(2)o(2) concentrations and localization throughout a year were analysed. h(2)o(2) changes in response to environmental conditions in parallel with changes in abscisic acid (aba) and oxidative stress markers, together with lignin accumulation, xylem and sclerenchyma diffe ...200919043066
enhanced determination of abscisic acid (aba) and abscisic acid glucose ester (aba-ge) in cistus albidus plants by liquid chromatography-mass spectrometry in tandem mode.an improved, quick and simple method for the extraction and quantification of the phytohormones (+)-abscisic acid (aba) and its major glucose conjugate, abscisic acid glucose ester (aba-ge) in plant samples is described. the method includes the addition of deuterium-labeled internal standards to the leaves at the beginning of the extraction for quantification, a simple extraction/centrifugation process and the injection into the liquid chromatography-electrospray ionization-tandem mass spectrome ...200919167901
loss of flower bud vigour in the mediterranean shrub, cistus albidus l. at advanced developmental stages.to better understand aging in perennials, age-related changes in the physiology of leaves and flower buds of the mediterranean shrub, cistus albidus l. were evaluated. two groups of different ages (5 and 10 years old), both at advanced developmental stages but of similar size, were compared. total plant biomass, biomass produced per apical meristem and levels of cytokinins, abscisic acid and jasmonic acid in leaves and flower buds, as well as flower production, were measured. no differences in p ...201020522184
clarifying the role of fire heat and daily temperature fluctuations as germination cues for mediterranean basin obligate seeders.this study aims to determine the role that both direct effects of fire and subsequent daily temperature fluctuations play in the seed bank dynamics of obligate seeders from the mediterranean basin. the short yet high soil temperatures experienced due to passage of fire are conflated with the lower, but longer, temperatures experienced by daily fluctuations which occur after removing vegetation. these germination cues are able to break seed dormancy, but it is difficult to assess their specific l ...201323129044
perennially young: seed production and quality in controlled and natural populations of cistus albidus reveal compensatory mechanisms that prevent senescence in terms of seed yield and viability.the question of whether or not perennial plants senesce at the organism level remains unresolved. the aim of this study was to unravel whether or not plant age can influence the production and composition of seeds. flower and seed production was examined in 3-, 8-, and 13-year-old cistus albidus plants growing in experimental plots corresponding to the f2, f1, and f0 generations of the same population. furthermore, the phytohormone, fatty acid, and vitamin e content of the seeds was evaluated, a ...201424218328
morphological characterization of mycorrhizae formed between three terfezia species (desert truffles) and several cistaceae and aleppo pine.six cistaceae species, helianthemum ledifolium, helianthemum lippii, fumana procumbens, cistus albidus, cistus incanus, cistus salvifolius, and pinus halepensis (aleppo pine) were inoculated with three mycorrhizal desert truffles, terfezia leptoderma, terfezia boudieri, and terfezia claveryi under greenhouse conditions, on soil originating from desert truffle natural habitat in algeria. the syntheses have led to the formation of typical endomycorrhizae in annual cistaceae (h. ledifolium) and per ...201424384787
zeatin modulates flower bud development and tocopherol levels in cistus albidus (l.) plants as they age.in a previous study we showed that cistus albidus (l.) experiences an age-dependent decay in flower vigour correlated with a decline in trans-zeatin (tz) levels. in the present study we aimed to establish a causal relationship between these two phenomena. exogenous tz applied to plants grown under semi-controlled conditions did not rescue flower vigour; however, it accelerated flower development, but only in younger individuals. older plants showed lower tocopherol levels in flower buds, which w ...201524943106
redox proteomics and physiological responses in cistus albidus shrubs subjected to long-term summer drought followed by recovery.the interaction between enzymatic and non-enzymatic antioxidants, endogenous levels of aba and aba-ge, the rapid recuperation of photosynthetic proteins under re-watering as well the high level of antioxidant proteins in previously drought-stressed plants under re-watering conditions, will contribute to drought resistance in plants subjected to a long-term drought stress under mediterranean field conditions. this work provides an overview of the mechanisms of cistus albidus acclimation to long-t ...201525502480
temporal changes in soil water repellency after a forest fire in a mediterranean calcareous soil: influence of ash and different vegetation type.forest fires usually modify soil water repellency (swr), and its persistence and intensity show a high variability both in space and time. this research studies the evolution of swr in a mediterranean calcareous soil affected by a forest fire, which occurred in gorga (se spain) in july 2011, comparing the effect of the main vegetation cover between pine (pinus halepensis) and shrubs species (quercus coccifera, rosmarinus officinalis, cistus albidus, erica arborea and brachypodium retusum) and th ...201626432513
use of phytoremediated sediments dredged in maritime port as plant nursery growing media.we evaluated the potential of a phytoremediated sediment (tr) dredged from maritime port as peat-free growth substrate for seven ornamental plants, in comparison with an untreated sediment (nt), in a greenhouse experiment. the studied plants were quercus ilex, photinia x fraseri, viburnum tinus, cistus albidus, raphiolepis indica, westringia fruticosa and teucrium fruticans. plant growth was monitored for ten months, and the changes in the physico-chemical properties, toxicity, microbial biomass ...201727307384
patterns of spatio-temporal distribution of winter chronic photoinhibition in leaves of three evergreen mediterranean species with contrasting acclimation responses.high irradiance and relatively low temperature, which characterize mediterranean winters, cause chilling stress in plants. downregulation of photosynthetic efficiency is a mechanism that allows plants to survive these conditions. this study aims to address whether this process shows a regular spatial pattern across leaf surface or not. three species (buxus sempervirens, cistus albidus and arctostaphylos uva-ursi) with contrasting responses to winter stress were studied. during 7 days, macro and ...201222150512
xylem hydraulic properties of roots and stems of nine mediterranean woody species.we studied the hydraulic architecture and water relations of nine co-occurring woody species in a spanish evergreen oak forest over the course of a dry season. our main objectives were to: (1) test the existence of a trade-off between hydraulic conductivity and security in the xylem, and (2) establish the safety margins at which the species operated in relation to hydraulic failure, and compare these safety margins between species and tissues (roots vs. stems). our results showed that the relati ...200224599365
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