Publications

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induction of cross-reactive serum neutralizing antibody to human rotavirus in calves after in utero administration of bovine rotavirus.sera from calves infected in utero or postnatally with bovine rotavirus ncdv or postnatally with human rotavirus d (serotype 1) were tested by plaque reduction neutralization assay for antibody to bovine rotavirus and to three serotypes of human rotavirus. homologous antibody developed in all animals, but antibody to heterologous rotaviruses developed mainly in animals exposed in utero to bovine rotavirus. the development of heterologous antibody may explain the immunological implications for cr ...19836313744
the genome segments of a group d rotavirus possess group a-like conserved termini but encode group-specific proteins.rotaviruses are a leading cause of viral acute gastroenteritis in humans and animals. they are grouped according to gene composition and antigenicity of vp6. whereas group a, b, and c rotaviruses are found in humans and animals, group d rotaviruses have been exclusively detected in birds. despite their broad distribution among chickens, no nucleotide sequence data exist so far. here, the first complete genome sequence of a group d rotavirus (strain 05v0049) is presented, which was amplified usin ...201020631147
prevalence of histopathological intestinal lesions and enteric pathogens in dutch commercial broilers with time.intestinal disease has a major impact on the broiler industry due to economic and welfare reasons. intestinal disease might occur due to a large number of reasons varying from well-defined pathogens to non-specific enteritis and complex syndromes. however, knowledge about the nature of intestinal disease and presence of enteric viruses in the dutch broiler industry is largely absent. therefore, a large-scale field study, in which 98 broiler flocks from 86 farms were sampled weekly, was started t ...201727557362
characterization of a group d rotavirus.a rotavirus isolated from the intestinal contents of commercial ring-neck pheasant chicks was determined to be a group d rotavirus based on its rna electropherotype and its serologic relatedness to known turkey group a and d rotaviruses using immune electron microscopy and agar-gel immunodiffusion assays. further characterization revealed a buoyant density in cesium chloride of 1.347 g/cm3 for double-shelled particles and 1.365 g/cm3 for single-shelled particles. the average size for the pheasan ...19938257366
a small round virus associated with enteritis in turkey poults.in a natural outbreak of enteric disease in turkey poults, salmonella, group d rotavirus, astrovirus, and a small (18-24 nm) round virus were detected in the gut contents. except for the small virus, the pathogenic potential of the other agents is recognized. in experiments, the small round virus was shown to be transmissible and pathogenic in specific-pathogen-free turkey poults.19902146946
detection, epidemiology and characterization of vp6 and vp7 genes of group d rotavirus in broiler chickens.rotaviruses infect humans and animals and are classified into eight groups (a to h). group d rotavirus (rvd) has been described in birds, although relatively few reports are available. the present study focused on rvd, including epidemiological and molecular aspects of samples collected from broiler chickens in the state of pará, brazil. a total of 85 faecal samples were collected between 2008 and 2011 from 37 chicken farms located in eight different municipalities. the viral double-stranded rna ...201424875189
detection of avian group d rotavirus using the polymerase chain reaction for the vp6 gene.group d rotaviruses (rvs-d) have been documented in birds and, while they may be common in these animals, few molecular studies are available for this specific group. in this study, specific primers for the gene that encodes for the rvs-d vp6 protein were designed and used in a reverse transcription polymerase chain reaction (rt-pcr). thirty pools of samples were tested by polyacrylamide gel electrophoresis (page) yielding a 30% (9/30) positivity. these pools were subjected subsequently to rt-pc ...201222820073
analysis of rotavirus species diversity and evolution including the newly determined full-length genome sequences of rotavirus f and g.rotaviruses are a leading cause of viral acute gastroenteritis in humans and animals. eight different rotavirus species (a-h) have been defined based on antigenicity and nucleotide sequence identities of the vp6 gene. here, the first complete genome sequences of rotavirus f (strain 03v0568) and g (strain 03v0567) with lengths of 18,341 and 18,186bp, respectively, are described. both viruses have open reading frames for rotavirus proteins vp1 to vp7 and nsp1 to nsp5 located at the 11 genome segme ...201323237956
morphogenesis of enteric lesions induced by group d rotavirus in ringneck pheasant chicks (phasianus colchicus).eight day-old male and female ringneck pheasants (phasianus colchicus) were inoculated with group d rotavirus and necropsied at 4, 7, and 11 days post-inoculation. the intestinal tracts were examined by light and electron microscopic and immunohistochemical methods. by 4 days post-inoculation, 2/3 (66%) inoculated birds were stunted and had diarrhea and dilated intestines. intestinal villi were shortened, and many villous enterocytes were partially detached from the lamina propria. crypts were h ...19948140729
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