the first human case of rickettsia tamurae infection in japan.a case of rickettsia tamurae infection in japan is reported. a 76-year-old japanese male had a tick bite which developed to local skin inflammation on his left leg. anti-rickettsia antibodies were detected in his serum, and r. tamurae dna was identified in his blood, the lesional skin, and the tick.201121503163
rickettsia species infecting amblyomma ticks from an area endemic for brazilian spotted fever in brazil.this study reports rickettsial infection in amblyomma cajennense and amblyomma dubitatum ticks collected in an area of the state of minas gerais, brazil, where brazilian spotted fever is considered endemic. for this purpose, 400 adults of a. cajenennse and 200 adults of a. dubitatum, plus 2,000 larvae and 2,000 nymphs of amblyomma spp. were collected from horses and from the vegetation. the ticks were tested for rickettsial infection through polymerase chain reaction (pcr) protocols targeting po ...201122166385
isolation of the rickettsial agent genetically similar to candidatus rickettsia kotlanii, from haemaphysalis megaspinosa in japan.two rickettsial isolates, hm-1 and hm-2, were isolated from haemaphysalis megaspinosa collected in japan in 2006 and 2011, respectively. the isolates were analyzed by dna sequences of the outer membrane protein a gene, the outer membrane protein b gene, the citrate synthase gene, the genus rickettsia-specific outer membrane protein 17-kda gene, the 16s ribosome rna gene, and the ps120 protein gene ("gened"). hm-1 was identified as rickettsia tamurae. hm-2 matched most closely with 'candidatus ri ...025229707
investigation of tick-borne bacteria (rickettsia spp., anaplasma spp., ehrlichia spp. and borrelia spp.) in ticks collected from andean tapirs, cattle and vegetation from a protected area in ecuador.ixodid ticks play an important role in the transmission and ecology of infectious diseases. information about the circulation of tick-borne bacteria in ticks is lacking in ecuador. our aims were to investigate the tick species that parasitize andean tapirs and cattle, and those present in the vegetation from the buffer zone of the antisana ecological reserve and cayambe-coca national park (ecuador), and to investigate the presence of tick-borne bacteria.201525616567
molecular detection of rickettsia, anaplasma, coxiella and francisella bacteria in ticks collected from artiodactyla in thailand.a total of 79 ticks collected from sambar deer (cervus unicolor), barking deer (muntiacus muntjak) and wild boar (sus scrofa) were examined by pcr for the presence of rickettsia, anaplasma, coxiella, and francisella bacteria. of the 79 ticks, 13% tested positive for rickettsia, 15% tested positive for anaplasma, 4% tested positive for coxiella, and 3% tested positive for francisella. interestingly, triple infection with anaplasma, rickettsia and francisella was determined in a dermacentor auratu ...201626934997
identification of spotted fever group rickettsia species by polymerase chain reaction-restriction fragment length polymorphism analysis of the sca4 gene.dna samples from four rickettsial species in japan--r. japonica aoki, r. asiatica io-1, r. helvetica ip-1, and rickettsia tamurae at-1--were analyzed by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) in which a portion of the sca4 gene was amplified and restriction digested with the mspi enzyme. each spotted fever group rickettsial species exhibited unique restriction profiles after mspi digestion. to examine the possibility of applying pcr-rflp analysis to field s ...200919485769
detection of rickettsia and anaplasma from hard ticks in thailand.we collected a total of 169 adult hard ticks and 120 nymphs from under the leaves of plants located along tourist nature trails in ten localities. the results present data examining the vector competence of ticks of different genera and the presence of rickettsia and anaplasma species. the ticks belonged to three genera, amblyomma, dermacentor, and haemaphysalis, comprising 11 species. rickettsia bacteria were detected at three collection sites, while anaplasma bacteria were detected at only one ...201526611960
evidence for the presence of francisella and spotted fever group rickettsia dna in the tick amblyomma fimbriatum (acari: ixodidae), northern territory, australia.ticks (n = 252) were collected from five wild-caught reptile species during routine trapping in the djukbinj national park and fogg dam reserve, northern territory, australia. pooling of ticks (one to four ticks per pool), according to sex or host animal, resulted in 187 samples used for screening for the presence of rickettsia species via molecular methods. rickettsia dna was detected via the amplification of the glta, ompa, and ompb genes in 57 (34%) of the 187 tick samples, all of which conta ...200919645299
molecular evidence of potential novel spotted fever group rickettsiae, anaplasma and ehrlichia species in amblyomma ticks parasitizing wild snakes.amblyomma ticks parasitize a wide range of animals in tropical regions. this study describes the identification of amblyomma ticks from wild snakes in malaysia and the detection of potential human pathogens such as rickettsia, anaplasma, ehrlichia and bartonellae in the ticks.201525889376
molecular detection of rickettsia species in amblyomma ticks collected from snakes in thailand.some reptile ticks are potential vectors of pathogens such as spotted fever group (sfg) rickettsiae. here, we report for the first time in detail the molecular evidence, dna sequences and phylogenetic studies, for the presence of rickettsia spp. in amblyomma ticks (amblyomma helvolum and amblyomma varanense) from snakes in thailand. a total of 24 tick samples was collected from 4 snake species and identified. a phylogenetic analysis inferred from the partial sequences of the glta gene indicated ...201425027232
genome sequence of rickettsia tamurae, a recently detected human pathogen in japan.rickettsia tamurae is a member of the spotted fever group rickettsiae, which was reported in 2011 to cause human infections in japan. we report the draft genome sequence of r. tamurae strain at-1(t), isolated from amblyomma testudinarium ticks.201425189579
microorganisms in ticks (acari: ixodidae) collected on marsupials and rodents from santa catarina, paraná and mato grosso do sul states, brazil.information about tick fauna and monitoring of pathogen prevalences in ticks (acari: ixodidae) in various habitat types can enhance knowledge about the epidemiology of tick-borne pathogens in brazil. this work shows the results of a study of tick parasitism of wild rodents and marsupials collected in seven localities in the southern part of brazil, within atlantic forest and cerrado biomes. a total of 61 ticks were collected from small mammals, and after identification to the species level, the ...201727769655
rickettsia tamurae sp. nov., isolated from amblyomma testudinarium ticks.rickettsia sp. strain at-1(t) was isolated from amblyomma testudinarium ticks in japan in 1993. comparative analysis of sequences obtained from 16s rrna, glta, ompa, ompb and sca4 gene fragments demonstrated those from at-1(t) to be markedly different from those of other members of the spotted fever group. using mouse serotyping, it was also observed that rickettsia sp. strain at-1(t) was different from other rickettsia species with validly published names. such genotypic and phenotypic characte ...200616825648
detection of rickettsia tamurae dna in ticks and wild boar (sus scrofa leucomystax) skins in shimane prefecture, japan.we used 24 wild boars trapped from december 2009 to january 2010 and a further 65 from july 2010 to august 2010 in misato town, shimane prefecture, japan. we collected blood, spleens, skins and ticks from the wild boars, which were examined for rickettsial infections using polymerase chain reaction (pcr) primers for the genes rickettsial 17-kda antigen and citrate synthase (glta). we amplified rickettsia tamurae at-1 dna from the tick amblyomma testudinarium and from wild boar skins where ticks ...201323075922
rickettsia sp. strain colombianensi (rickettsiales: rickettsiaceae): a new proposed rickettsia detected in amblyomma dissimile (acari: ixodidae) from iguanas and free-living larvae ticks from vegetation.from january to december 2009, 55 amblyomma dissimile (koch) ticks removed from iguanas in the municipality of monteria and 3,114 ticks [458 amblyomma sp. larvae, 2,636 rhipicephalus microplus (canestrini) larvae and 20 amblyomma sp. nymphs] collected over vegetation in los cordobas were included in the study. the ticks were pooled into groups from which dna was extracted. for initial screening of rickettsia sp., each pool was analyzed by glta real-time polymerase chain reaction (pcr). positive ...201222897060
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