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isolation and characterization of a carbendazim-degrading rhodococcus sp. djl-6.bacterium djl-6, capable of degrading carbendazim, was isolated by continuous enrichment culture originating from carbendazim-treated soil. the isolate was identified as rhodococcus sp. according to its phenotypic features, physiologic and biochemical characteristics, and phylogenetic analysis. the strain could use carbendazim as sole carbon or nitrogen source. it showed a high average degradation rate of 55.56 mg . l(-1) . d(-1) in m9 medium amended with carbendazim. high-pressure liquid chroma ...200616775791
rhodococcus qingshengii sp. nov., a carbendazim-degrading bacterium.a gram-positive, aerobic, non-motile, mesophilic strain, djl-6(t), able to degrade carbendazim, was isolated from a carbendazim-contaminated soil sample from jiangsu province, china. the taxonomic position of this isolate was analysed by using a polyphasic approach. chemotaxonomic analysis including peptidoglycan type, diagnostic sugar composition, fatty acid profile, menaquinones, polar lipids and mycolic acids showed that the characteristics of strain djl-6(t) were in good agreement with those ...200718048720
rhodococcus jialingiae sp. nov., an actinobacterium isolated from sludge of a carbendazim wastewater treatment facility.a novel carbendazim-degrading actinobacterium, designated djl-6-2(t), was isolated from the sludge of a carbendazim wastewater treatment facility in jiangsu province, china. the morphological and chemotaxonomic properties of the isolate were typical of members of the genus rhodococcus. strain djl-6-2(t) formed a coherent cluster with rhodococcus qingshengii djl-6(t), rhodococcus baikonurensis dsm 44587(t), rhodococcus erythropolis dsm 43066(t) and rhodococcus globerulus dsm 43954(t) in 16s rrna ...201019651732
pseudo-membranes on internal organs associated with rhodococcus qingshengii infection in atlantic salmon (salmo salar).this paper describes a pathological condition in intensive reared atlantic salmon (salmo salar), restricted to the appearance of pseudo-membranes covering internal organs (i.e. spleen, liver, heart and others) associated with the presence of large numbers of a gram-positive bacteria. isolate 79043-3, obtained as pure culture from affected fish, was subjected to a polyphasic taxonomic study in order to determine its exact taxonomic position, as well as to experimental challenges leading to determ ...201120580498
biosynthesis of 2-amino-2,3-dimethylbutyramide by nitrile hydratase from a newly isolated cyanide-resistant strain of rhodococcus qingshengii.cells of a new isolate of rhodococcus qingshengii harboring nitrile hydratase converted 2-amino-2,3-dimethylbutyronitrile into 2-amino-2,3-dimethylbutyramide (adba). cells also hydrated a broad range of substrates including saturated, unsaturated and cyclic aliphatic nitriles. the microorganism tolerated kcn and adba up to 5 mm and 40 g/l, respectively. at 10°c, adba reached 33.8 g/l (yield 84.5%) which was 2.5 times that at 30°c.201121516314
enhancement of microbial biodesulfurization via genetic engineering and adaptive evolution.in previous work from our laboratories a synthetic gene encoding a peptide ("sulpeptide 1" or "s1") with a high proportion of methionine and cysteine residues had been designed to act as a sulfur sink and was inserted into the dsz (desulfurization) operon of rhodococcus erythropolis igts8. in the work described here this construct (dszas1bc) and the intact dsz operon (dszabc) cloned into vector presx under control of the (rhodococcus) kstd promoter were transformed into the desulfurization-negat ...201728060828
classification of strain ccm 4446t as rhodococcus degradans sp. nov.strain ccm 4446t, with notable biodegradation capabilities, was investigated in this study in order to elucidate its taxonomic position. chemotaxonomic analyses of quinones, polar lipids, mycolic acids, polyamines and the diamino acid of the cell-wall peptidoglycan corresponded with characteristics of the genus rhodococcus. phylogenetic analysis, based on the 16s rrna gene sequence, assigned strain ccm 4446t to the genus rhodococcus and placed it in the rhodococcus erythropolis 16s rrna gene cla ...201526385412
draft genome sequence of the piezotolerant and crude oil-degrading bacterium rhodococcus qingshengii strain tuhh-12.we report here the draft genome sequence of rhodococcus qingshengii strain tuhh-12. the ability of this piezotolerant bacterium to grow on crude oil and tetracosane as sole carbon sources at 150 × 10(5) pa makes it useful in studies of hydrocarbon degradation under simulated deep-sea conditions.201525858843
effect of high pressure on hydrocarbon-degrading bacteria.the blowout of the deepwater horizon in the gulf of mexico in 2010 occurred at a depth of 1500 m, corresponding to a hydrostatic pressure of 15 mpa. up to now, knowledge about the impact of high pressure on oil-degrading bacteria has been scarce. to investigate how the biodegradation of crude oil and its components is influenced by high pressures, like those in deep-sea environments, hydrocarbon degradation and growth of two model strains were studied in high-pressure reactors. the alkane-degrad ...201425401077
effect of the anode feeding composition on the performance of a continuous-flow methane-producing microbial electrolysis cell.a methane-producing microbial electrolysis cell (mec) was continuously fed at the anode with a synthetic solution of soluble organic compounds simulating the composition of the soluble fraction of a municipal wastewater. the mec performance was assessed at different anode potentials in terms of chemical oxygen demand (cod) removal efficiency, methane production, and energy efficiency. as a main result, about 72-80% of the removed substrate was converted into current at the anode, and about 84-86 ...201524994102
sequence analysis of 16s rrna, gyrb and cata genes and dna-dna hybridization reveal that rhodococcus jialingiae is a later synonym of rhodococcus qingshengii.the results of 16s rrna, gyrb and cata gene sequence comparisons and reasserted dna-dna hybridization unambiguously proved that rhodococcus jialingiae wang et al. 2010 and rhodococcus qingshengii xu et al. 2007 represent a single species. on the basis of priority r. jialingiae must be considered a later synonym of r. qingshengii.201424408950
draft genome sequence of rhodococcus qingshengii strain bks 20-40.we report the 5.8-mb genome sequence of rhodococcus qingshengii strain bks 20-40, isolated from a palm tree rhizosphere soil sample from bhitarkanika national park, odisha, india. the strain is capable of degrading cholesterol moiety. the draft genome of strain bks 20-40 consists of 6,601,618 bp, with 62.4% g+c content.201323538906
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