| e6/e7 expression of human papillomavirus type 20 (hpv-20) and hpv-27 influences proliferation and differentiation of the skin in uv-irradiated skh-hr1 transgenic mice. | the functional role of uv irradiation, in combination with the e6 and e7 proteins of the cutaneous human papillomavirus (hpv) types in the malignant conversion of benign papillomatous lesions, has not been elucidated. transgenic skh-hr1 hairless mice expressing hpv-20 and hpv-27 e6 and e7 proteins in the suprabasal compartment were generated and exposed to chronic uv irradiation. histological and immunohistochemical examination of skin samples revealed enhanced proliferation of the epidermal lay ... | 2006 | 16971438 |
| beta-papillomaviruses in anogenital hairs plucked from healthy individuals. | a total of 150 specimens of anogenital hairs plucked from the scrotal, pubic, and perianal region of 51 immunocompetent healthy male individuals were tested for the presence of beta-papillomaviruses (beta-hpv) using the nested m(a)/h(a) polymerase chain reaction. beta-hpv were found in a total of 38 (25.3%) of 150 hair samples. according to the sampling sites, beta-hpv were detected in 18/51 (35.3%), 13/50 (26.0%), and 7/49 (14.3%) plucked hair samples obtained from the pubic, scrotal, and peria ... | 2006 | 17063512 |
| alpha- and betapapillomavirus e6/e7 genes differentially modulate pro-inflammatory gene expression. | keratinocytes, the target cell of human papillomavirus (hpv) infection, can produce numerous cytokines and pro-inflammatory molecules which are important for the generation of an effective immune response. how this biological response, which involves the tumor stroma, is affected by the hpv oncoproteins within the epithelial cell itself is not clear. here it is shown that oncoproteins of different hpv genotypes (alpha- versus beta-hpv genus) alter the expression of pro-inflammatory molecules in ... | 2007 | 17079045 |
| betapapillomaviruses frequently persist in the skin of healthy individuals. | infections with human papillomaviruses (hpvs) belonging to the genus betapapillomavirus have been linked to the development of non-melanoma skin cancer. although persistence is expected, systematic investigation of this aspect of betapapillomavirus (beta-pv) infection has not been conducted. this study investigated the prevalence and persistence of 25 known beta-pv types in the skin of immunocompetent individuals. over a 2 year period, eight consecutive plucked eyebrow hair samples taken from 23 ... | 2007 | 17412978 |
| the anti-papillomavirus activity of human and bovine lactoferricin. | human papillomavirus (hpv) cause common warts, laryngeal papilloma and genital condylomata and is necessary for the development of cervical cancer. we have previously found that lactoferrin has antiviral activity against hpv-16 and others have demonstrated that lactoferricin, an n-terminal fragment of lactoferrin, has inhibitory activities against several viruses. two cell lines and two virus types, hpv-5 and hpv-16, were used to study if lactoferrin and lactoferricin could inhibit hpv pseudovir ... | 2007 | 17481742 |
| complete genome and phylogenetic position of bovine papillomavirus type 7. | six bovine papillomavirus (bpv) types and 16 putative bpv types have been reported previously. here, the complete genome sequence of bapv6, a novel putative bpv type isolated from cattle in japan, was determined by using multiple-primed rolling-circle amplification. the genome consisted of 7412 bp (g+c content of 46 mol%) that encoded five early (e1, e2, e4, e6 and e7) and two late (l1 and l2) genes, but did not encode the e5 gene. the e6 protein contained a non-consensus cxxc(x)(33)cxxc and a c ... | 2007 | 17554025 |
| development of a sensitive and specific multiplex pcr method combined with dna microarray primer extension to detect betapapillomavirus types. | emerging lines of evidence indicate that the cutaneous human papillomavirus (hpv) types that belong to the genus betapapillomavirus (beta hpv) are involved in the development of nonmelanoma skin cancer. unlike the situation for mucosal hpv types, highly sensitive and reliable methods to identify characterized cutaneous hpv types in a single assay are limited. here, we describe a novel one-shot method for the detection of all characterized beta hpv types, namely, hpv type 5 (hpv5), 8, 9, 12, 14, ... | 2007 | 17581938 |
| extension of the typing in a general-primer-pcr reverse-line-blotting system to detect all 25 cutaneous beta human papillomaviruses. | beta-papillomaviruses (pv) seem to be involved in the pathogenesis of cutaneous squamous cell carcinoma and its early stage actinic keratosis. in this study, typing was extended of a previously described consensus primer-mediated beta- and gamma-cutaneous hpv pcr method followed by reverse-line-blotting (bgc-pcr/rlb) to detect all 25 known beta-pv and to examine their prevalence in actinic keratosis. the typing format of the bgc-pcr assay was extended by adding hybridization probes of six beta-p ... | 2007 | 17604130 |
| sun-related factors, betapapillomavirus, and actinic keratoses: a prospective study. | to examine prospectively the relationship among sun exposure, betapapillomavirus, and development of actinic keratoses. | 2007 | 17638729 |
| cutaneous human papillomaviruses found in sun-exposed skin: beta-papillomavirus species 2 predominates in squamous cell carcinoma. | a spectrum of cutaneous human papillomaviruses (hpvs) is detectable in nonmelanoma skin cancers, as well as in healthy skin, but the significance that the presence of these types of hpv dna has for the pathogenesis of skin cancer remains unclear. | 2007 | 17703418 |
| genetic diversity of cutaneous human papillomaviruses. | human papillomaviruses (hpvs) of the genera betapapillomavirus and gammapapillomavirus are common on human skin. sequencing of subgenomic amplicons of cutaneous hpvs has revealed a large number of novel putative hpv types within these genera. phylogenetic analysis based on these amplicons revealed 133 putative hpv types with <90 % sequence identity to any known hpv type or to each other. as there are already 34 characterized hpv types described within the genera betapapillomavirus and gammapapil ... | 2007 | 17872517 |
| common variable immunodeficiency syndrome associated with epidermodysplasia verruciformis. | we report the association of common variable immunodeficiency syndrome with epidermodysplasia verruciformis (ev) manifesting as flat warts and squamous cell carcinomas in a 21-year-old hispanic woman. human papillomavirus (hpv) typing by polymerase chain reaction using an ev-hpv primer system, cloning, and sequencing detected hpv-8 and hpv-23 dnas in the biopsy sample. our case is the second reported case of common variable immunodeficiency syndrome associated with ev. | 2007 | 17902733 |
| loss of p53 or p73 in human papillomavirus type 38 e6 and e7 transgenic mice partially restores the uv-activated cell cycle checkpoints. | we have previously shown that human keratinocytes expressing e6 and e7 from the cutaneous human papillomavirus (hpv) type 38 have high levels of a specific form of p53, which in turn activate the transcription of deltanp73 gene. expression of hpv38 e6 and e7 in mouse skin also promotes p53 and deltanp73 accumulation. interestingly, keratinocytes of these mice do not undergo cell cycle arrest after skin ultraviolet (uv) irradiation. here, we provide several lines of evidence that deltanp73 expres ... | 2008 | 18026133 |
| the e6 oncoproteins from human betapapillomaviruses differentially activate telomerase through an e6ap-dependent mechanism and prolong the lifespan of primary keratinocytes. | human papillomaviruses (hpvs) belonging to the betapapillomavirus genus have recently been implicated in squamous cell carcinomas of the skin, though the mechanisms by which they initiate carcinogenesis are unclear. we show that human foreskin keratinocytes (hfks) expressing several betapapillomavirus e6 (beta-e6) proteins display life span extension, but not to the extent seen in hfks expressing hpv type 16 e6 (16e6). additionally, we demonstrate that beta-e6 proteins can differentially activat ... | 2008 | 18256157 |
| multiple facial seborrheic keratosis-like lesions in a young woman with epidermodysplasia verruciformis. | | 2008 | 18412865 |
| beta-papillomavirus infection and skin cancer. | the development of highly sensitive pcr techniques and multiplex bead-based luminex platforms has accelerated the search for a specific role of human papilloma viruses in the development of squamous cell carcinoma. human papillomaviruses are most likely indirectly involved in this process by facilitating uv-related carcinogenesis via preventing uv-induced apoptosis or impairing dna repair, but other mechanisms are also possible. | 2008 | 18478011 |
| beta-papillomaviruses and psoriasis: an intra-patient comparison of human papillomavirus carriage in skin and hair. | human papillomaviruses (hpvs) of the beta genus (beta-pv), especially hpv5 and hpv36, are proposed to play a pathogenic role in psoriasis, but many previous studies have failed to control for potential confounders, including treatment. | 2008 | 18510676 |
| e6 proteins from multiple human betapapillomavirus types degrade bak and protect keratinocytes from apoptosis after uvb irradiation. | human papillomavirus (hpv) types from the beta genus (beta-hpvs) have been implicated in the development of skin cancer. a potentially important aspect of their carcinogenic role is the ability of the e6 protein to degrade the proapoptotic family member bak, which gives cells the ability to survive uv damage. however, it is unknown if the ability to degrade bak is limited to certain beta-hpv types or whether e6 expression in keratinocytes affects other proteins important for apoptosis signaling. ... | 2008 | 18715924 |
| measures of cutaneous human papillomavirus infection in normal tissues as biomarkers of hpv in corresponding nonmelanoma skin cancers. | cutaneous human papillomavirus (hpv) may be associated with the development of nonmelanoma skin cancer (nmsc), as suggested by reports of hpv dna in nmsc tumors. hpv has also been investigated as an nmsc risk factor in epidemiologic studies, although findings vary across studies that used different biomarkers of hpv infection in normal tissues. to identify appropriate biomarkers for use in future epidemiologic studies, we conducted a sampling validation study. nmsc tumor tissue was obtained from ... | 2008 | 18729188 |
| specific betapapillomaviruses associated with squamous cell carcinoma of the skin inhibit uvb-induced apoptosis of primary human keratinocytes. | epidemiological studies have shown an association between infections by specific betapapillomaviruses, such as human papillomavirus (hpv) types 5 and 8, and cutaneous squamous cell carcinoma (scc). the role of betapapillomaviruses in the development of cutaneous scc is, however, still enigmatic. the ability to inhibit uvb-induced apoptosis, as demonstrated for hpv5 in vitro, may be important in this respect, as survival of dna-damaged and mutated cells increases the risk of transformation. the a ... | 2008 | 18753241 |
| altered expression of uvb-induced cytokines in human papillomavirus-immortalized epithelial cells. | keratinocytes can be induced to produce cytokines by exogenous stimuli, such as uvb, and dysregulation of this production has been described in various skin diseases, including cancer. in this study, we compared the effect of uvb on the secretion of several cytokines involved in inflammation by human keratinocytes immortalized or not with human papillomavirus (hpv)16 or hpv38 at the mrna and protein levels. we show that expression of the hpv e6/e7 oncoproteins influences not only the basal cytok ... | 2008 | 18796714 |
| four novel human betapapillomaviruses of species 2 preferentially found in actinic keratosis. | recent studies have suggested an association between human papillomaviruses (hpvs), particularly species 2 members of the genus betapapillomavirus, and squamous cell carcinoma (scc) of the skin. as most of these viruses are uncharacterized, molecular characterization and epidemiology are needed to advance our understanding of their significance in carcinogenesis. this study determined the complete genomes of four betapapillomaviruses of species 2 from skin lesions designated hpv-107, -110 and -1 ... | 2008 | 18796715 |
| high beta-hpv dna loads and strong seroreactivity are present in epidermodysplasia verruciformis. | epidermodysplasia verruciformis (ev) is a rare disease, characterized by cutaneous warts and associated with a strong predisposition to beta-genus human papillomavirus (hpv). earlier studies reported high copy numbers of hpv-dna in nearly all skin tumors from ev patients, but neither hpv replication status in non-lesional skin nor anti-hpv seroreactivity in these patients have been reported yet. we therefore performed a comprehensive viral load analysis for the more common beta-hpv types on skin ... | 2009 | 18923444 |
| betapapillomaviruses: innocent bystanders or causes of skin cancer. | human papillomaviruses (hpv) are found in almost all squamous epithelia where they can cause hyperproliferative disease of mucosa and skin. mucosal hpv types, such as hpv6 and hpv16, are known to cause anogenital warts and dysplasia or neoplasia, respectively. these hpv types have been studied extensively, and for some of them recently preventive vaccines have become available. although hpv that populate the skin were the first identified hpv types, knowledge of the pathogenicity of hpv in the c ... | 2008 | 18986829 |
| intrafamilial transmission and family-specific spectra of cutaneous betapapillomaviruses. | cutaneous human betapapillomaviruses (beta-hpvs) are widespread in the general population and have been associated with skin cancer. to evaluate the impact of continuous person-to-person contact within families on an individual's beta-hpv type spectrum, we collected serial skin swab samples from parents and children from 10 families. all participants were found to be beta-hpv dna positive, with 1 to 13 types at study entry (median, 4.0 types). initial and cumulative (2 to 16 types) hpv type mult ... | 2009 | 18987132 |
| serological relationship between cutaneous human papillomavirus types 5, 8 and 92. | evidence of a possible association of cutaneous human papillomavirus (hpv) types, especially members of the genus betapapillomavirus, and the development of non-melanoma skin cancer (nmsc) is accumulating. vaccination with virus-like particles (vlps) consisting of self-assembled l1, the major capsid protein, has been introduced to control anogenital hpv infection. this study examined the serological relationship between betapapillomavirus (beta-pv) types 5 and 8 and the new type hpv-92, which ha ... | 2009 | 19088282 |
| genomic characterization of the first insectivoran papillomavirus reveals an unusually long, second non-coding region and indicates a close relationship to betapapillomavirus. | knowledge about biological diversity is the prerequisite to reliably reconstruct the evolution of pathogens such as papillomaviruses (pv). however, complete genomes of non-human pv have only been cloned and sequenced from 8 out of 18 orders within the placentalia, although the host-specific variety of pv is considered much larger. we isolated and sequenced the complete genome of the first insectivoran pv type from hair follicle cells of the european hedgehog (erinaceus europaeus), designated ehp ... | 2009 | 19218207 |
| macaca fascicularis papillomavirus type 1: a non-human primate betapapillomavirus causing rapidly progressive hand and foot papillomatosis. | papillomaviruses (pvs) are a group of small, non-enveloped dna viruses that cause mucosal or cutaneous neoplasia in a variety of animals. whilst most papillomas will regress spontaneously, some may persist or undergo malignant transformation. in this study, aggressive, persistent and extensive warts were observed on the hands and feet of a cynomolgus macaque (macaca fascicularis). the presence of pv in the wart biopsies was identified by immunohistochemistry and pcr amplification of pv dna. the ... | 2009 | 19264664 |
| prevalence and associated factors of betapapillomavirus infections in individuals without cutaneous squamous cell carcinoma. | betapapillomavirus (betapv) infections are often associated with squamous-cell carcinoma (scc) and the prevalence of betapv infections in (immunosuppressed) scc patients is known to be high. the distribution and possible associated factors of betapv infections in the general population, however, are largely unknown. to address this issue, betapv infection was studied in 1405 scc-free immunocompetent (n=845) and immunosuppressed (n=560) individuals from six countries of different latitudes. a sta ... | 2009 | 19321753 |
| characterization of seven novel human papillomavirus types isolated from cutaneous tissue, but also present in mucosal lesions. | seven novel human papillomavirus (hpv) types were isolated and characterized. hpv 94 is related most closely to hpv 10 and belongs to the genus alphapapillomavirus, whereas hpv 98, hpv 99, hpv 100, hpv 104, hpv 105 and hpv 113 all belong to the genus betapapillomavirus. these hpv types were isolated from and demonstrated in cutaneous tissue, but hpv 98, hpv 100, hpv 104 and hpv 113 were also detected in malignant oesophageal and oral lesions. the general prevalence of these hpv types in lesions ... | 2009 | 19386784 |
| presence of beta human papillomaviruses in nonmelanoma skin cancer from organ transplant recipients and immunocompetent patients in the west of scotland. | nonmelanoma skin cancer (nmsc) has been linked to cutaneous human papillomaviruses of the genus beta (betapv). | 2009 | 19416244 |
| detection of alpha- and beta-human papillomavirus (hpv) in cutaneous melanoma: a matched and controlled study using specific multiplex pcr combined with dna microarray primer extension. | there are few contradictory studies investigating the involvement of hpv in melanoma. we designed a controlled study to evaluate the hpv dna prevalence in melanoma. one hundred patients with cutaneous malignant melanoma diagnosed between 2002 and 2006 were included. complementary wide excision (healthy skin) was performed in 85 patients and was used as internal control. after dna extraction, 68 different hpv types were studied using a multiplex pcr combined with microarray primer extension. we d ... | 2009 | 19469900 |
| prevalence and multiplicity of cutaneous beta papilloma viruses in plucked hairs depend on cellular dna input. | in view of the low loads of beta human papillomaviruses in skin samples, amounts of cellular dna used in qualitative pcr may become limiting for virus detection and introduce variations in prevalence and multiplicity. this issue was explored within the context of a multicentre study and increasing prevalence and multiplicity was found with increasing input amounts of cellular dna extracted from hair bulbs. to improve the quality and comparability between different epidemiologic studies ideally e ... | 2009 | 19591874 |
| interaction of the betapapillomavirus e2 tethering protein with mitotic chromosomes. | during persistent papillomavirus infection, the viral e2 protein tethers the viral genome to the host cell chromosomes, ensuring maintenance and segregation of the viral genome during cell division. however, e2 proteins from different papillomaviruses interact with distinct chromosomal regions and targets. the tethering mechanism has been best characterized for bovine papillomavirus type 1 (bpv1), where the e2 protein tethers the viral genome to mitotic chromosomes in complex with the cellular b ... | 2010 | 19846509 |
| betapapillomavirus infection profiles in tissue sets from cutaneous squamous cell-carcinoma patients. | human papillomaviruses from the genus beta (betapv) are a possible cause of cutaneous squamous cell carcinoma (scc). we compared the betapv infections in scc and in sets of cutaneous tissues collected from a series of individual scc patients to determine concordance and to assess the adequacy of eyebrow hairs as noninvasive markers of betapv infection. biopsies of scc tumors, perilesional tissue, normal skin from the mirror image of nonfacial scc and plucked eyebrow hairs were collected from 21 ... | 2010 | 19856311 |
| persistence of betapapillomavirus infections as a risk factor for actinic keratoses, precursor to cutaneous squamous cell carcinoma. | human papillomaviruses from the beta genus (betapv) are a possible cause of cutaneous squamous cell carcinoma (scc). we assessed the extent to which betapv infections persisted long-term in a subtropical australian community and whether betapv persistence is positively associated with actinic keratoses, precursor for scc. eyebrow hairs were collected from 171 participants of the community-based nambour skin cancer study in 1996 and 2003. hair samples were tested for the presence of dna from 25 d ... | 2009 | 19903846 |
| human papillomavirus 5 and 8 e6 downregulate interleukin-8 secretion in primary human keratinocytes. | human papillomaviruses (hpvs) of the genus betapapillomavirus appear to be involved in the early stages of skin cancer development, since both the prevalence and viral load are higher in precancerous actinic keratoses than in skin cancers. interleukin-8 (il-8) is an inflammatory cytokine that serves to alert the surrounding tissue after uv-induced damage. we examined the effects of the e2, e6 and e7 proteins of hpv8 and the e6 proteins of various hpv genotypes on il-8 secretion from primary kera ... | 2010 | 20007354 |
| quantification of beta-human papillomavirus dna by real-time pcr. | quantitative pcr with hybridization probes allows the reliable quantification of viral dna sequences in clinical samples with a dynamic range and sensitivity that cannot be achieved with other methods. the technical background for the establishment of protocols is described and established protocols are presented to estimate the viral load per cell of frequently occurring betapapillomaviruses (hpv5, -8, -15, -20, -23, -24, -36 and -38) in skin tumors, healthy skin and hair bulbs. this approach a ... | 2010 | 20010722 |
| transmission of betapapillomaviruses between domestic partners in an australian community. | betapapillomaviruses may be associated with the development of cutaneous squamous cell carcinoma but little is known about their transmission. one suggestion is that they are transmitted through close skin contact. | 2010 | 20053583 |
| adenovirus type 5 e1a and e6 proteins of low-risk cutaneous beta-human papillomaviruses suppress cell transformation through interaction with foxk1/k2 transcription factors. | the adenovirus (adv) oncoprotein e1a stimulates cell proliferation and inhibits differentiation. these activities are primarily linked to the n-terminal region (exon 1) of e1a, which interacts with multiple cellular protein complexes. the c terminus (exon 2) of e1a antagonizes these processes, mediated in part through interaction with c-terminal binding proteins 1 and 2 (ctbp1/2). to identify additional cellular e1a targets that are involved in the modulation of e1a c-terminus-mediated activitie ... | 2010 | 20053746 |
| human alpha and beta papillomaviruses use different synonymous codon profiles. | human papillomaviruses use rare codons relative to their hosts. it has been theorized that this is a mechanism to allow the virus to escape immune surveillance. in the present study, we examined the codings of four major genes of 21 human alpha (mucosatropic) viruses and 16 human beta (cutaneous-tropic) viruses. we compared the codon usage of different genes from a given papillomavirus and also the same genes from different papillomaviruses. our data showed that codon usage was not always unifor ... | 2010 | 20157772 |
| an analysis of clustering of betapapillomavirus antibodies. | betapapillomaviruses (betapvs) may contribute to the aetiology of cutaneous squamous cell carcinoma. however, no high-risk types have yet been identified, possibly because the high frequency of co-infection prevents a straightforward analysis of the independent effects of individual viruses. this study aimed to determine whether specific virus types were more likely to co-occur than others, thereby reducing the number of parameters needed in statistical models. antibody data were analysed from c ... | 2010 | 20392895 |
| lack of association between the presence and persistence of betapapillomavirus dna in eyebrow hairs and betapapillomavirus l1 antibodies in serum. | betapapillomavirus (betapv) dna and seroresponses are highly prevalent in the general population and both are frequently used as infection markers in epidemiological studies to elucidate an association with cutaneous squamous cell carcinoma (scc). little is known about the natural history of betapv infection and the aspects of infection that drive antibody responses. to investigate the relationship between these markers, this study assessed whether the presence or persistence of betapv dna in ey ... | 2010 | 20444998 |
| enhanced human papillomavirus type 8 oncogene expression levels are crucial for skin tumorigenesis in transgenic mice. | human papillomavirus 8 (hpv8) is involved in skin cancer development in epidermodysplasia verruciformis patients. transgenic mice expressing hpv8 early genes (hpv8-cer) developed papillomas, dysplasias and squamous cell carcinomas. uva/b-irradiation and mechanical wounding of hpv8-cer mouse skin led to prompt papilloma induction in about 3 weeks. the aim of this study was to analyze the kinetics and level of transgene expression in response to skin irritations. transgene expression was already e ... | 2010 | 20451232 |
| detection of human papillomavirus dna in feline premalignant and invasive squamous cell carcinoma. | squamous cell carcinoma (scc) is the most common malignant cutaneous and oral neoplasm of cats. papillomavirus (pv) dna has been identified in a proportion of feline bowenoid in situ carcinomas (biscs), cutaneous sccs and a single oral scc, but its exact role in the pathogenesis remains unknown. in humans, it has been suggested that ultraviolet (uv) light and human pv (hpv) may act as cofactors in cutaneous scc carcinogenesis. little is known about the influence of uv light on pv prevalence in f ... | 2011 | 20609206 |
| genus beta human papillomaviruses and incidence of basal cell and squamous cell carcinomas of skin: population based case-control study. | to investigate the association between genus beta human papillomaviruses and the incidence of non-melanocytic skin cancer in the general population. | 2010 | 20616098 |
| novel betapapillomavirus associated with hand and foot papillomas in a cynomolgus macaque. | betapapillomavirus is a genus of papillomaviruses (pvs) commonly found in human skin and associated with both benign and malignant skin lesions. only 2 previous beta-pvs have been fully characterized in nonhuman species. this report describes a novel beta-pv, named macaca fascicularis pv type 2 (mfpv2), isolated from exophytic skin papillomas on the hands and feet of a 2-year-old male cynomolgus monkey (m. fascicularis). on histology the papillomas were composed of diffusely thickened epidermis ... | 2010 | 20921322 |
| differential regulation of human papillomavirus type 8 by interferon regulatory factors 3 and 7. | the genus β human papillomavirus (hpv) type 8 is associated with nonmelanoma skin cancer in patients with epidermodysplasia verruciformis, and evidence for its protumorigenic potential in the general population increases. to date, strategies to suppress genus β hpv infections are limited. interferon regulatory factors irf-3 and irf-7 play key roles in the activation of the innate immune response to viral infections. in this study, we show for the first time that both irf-3 and irf-7 regulate tra ... | 2010 | 20980500 |
| multicenter study of the association between betapapillomavirus infection and cutaneous squamous cell carcinoma. | human papillomaviruses (betapv) from the beta genus cannot be classified according to their oncogenicity due to a paucity of information. this study evaluates the association between betapv infection and cutaneous squamous cell carcinoma in conjunction with measures of uv exposure and susceptibility. we performed case-control studies in the netherlands, italy, and australia, countries with profoundly different uv exposures. the presence of 25 betapv types in eyebrow hair follicles was determined ... | 2010 | 21098702 |
| detection of merkel cell polyomavirus in epidermodysplasia-verruciformis-associated skin neoplasms. | background: epidermodysplasia verruciformis (ev) is a rare genodermatosis that is characterized by susceptibility to infection with specific human papillomavirus (hpv) genotypes. among polyomaviruses, the novel merkel cell polyomavirus (mcpyv) has been found in different epithelial skin neoplasias. | 2010 | 21099200 |
| [biology of epidermodysplasia verruciformis-associated hpv]. | the genus betapapillomavirus (betapv) presently comprises more than 40 virus types including the so-called epidermodysplasia verruciformis (ev)-associated hpv, which were originally detected in ev-patients by southern blot hybridization. betapv are ubiquitous in the general population and frequently establish themselves already during the first weeks of life. hair follicles are regarded as natural reservoir. about 25% of betapv detected in adults persist for at least 9 months. due to very low vi ... | 2011 | 21113568 |
| marked differences in betapapillomavirus dna and antibody prevalence between patients with psoriasis and those with atopic dermatitis. | recent studies revealed that betapapillomavirus (betapv) infections are highly prevalent. skin diseases such as psoriasis, characterized by keratinocyte hyperproliferation, and atopic dermatitis (ad), dominated by cutaneous inflammation, might have an impact on viral life cycle and immune response induction. | 2011 | 21155755 |
| e6 and e7 proteins from different beta-papillomaviruses types do not interfere in uvb-induced apoptosis of hacat keratinocytes. | beta-papillomaviruses (beta-hpv) have been linked to the development of skin cancer in humans. because both e6 and e7 proteins from beta-hpv have been involved in the potential carcinogenicity of these viruses, we investigated their role on uvb-induced apoptosis in hacat cell line. hacat cells have been transduced with both e6/e7 using a retroviral system and treated with prima-1. apoptosis was assessed by flow cytometry to measure mitochondrial membrane potential and dna fragmentation. hacat ke ... | 2011 | 21158941 |
| homozygosity for the c.917a→t (p.n306l) polymorphism in the ever2/tmc8 gene of two sisters with epidermodysplasia verruciformis lewandowsky-lutz originally described by wilhelm lutz. | epidermodysplasia verruciformis lewandowsky-lutz (ev) is a rare genodermatosis, characterised by development of numerous verrucous skin lesions caused by specific genotypes of human papillomaviruses belonging to the β-papillomavirus genus. the ev loci were mapped to chromosome 2p21-p24 (ev2) and 17q25 (ev1). on chromosome 17, 2 adjacent related genes--ever1/tmc6 and ever2/tmc8--were identified. we reinvestigated 2 patients originally described by wilhelm lutz in 1946 with the aim to document the ... | 2010 | 21196704 |
| human papillomavirus in brazilian women with and without cervical lesions. | human papillomavirus (hpv) high-risk (hr) types are the causal factor for cervical cancer and premalignant dysplasia. data on frequency of hpv types provide a basis to design and evaluate hpv prevention programs. taking into account the heterogeneity of hpv types across and within populations this study aims to access the hpv frequency in brazilian women. | 2011 | 21208414 |
| enhanced stefina and sprr2 expression during papilloma formation in hpv8 transgenic mice. | the human papillomavirus type 8 (hpv8) is associated with the development of non-melanoma skin cancer. transgenic mice expressing the complete early gene region of hpv8 (e6/e7/e1/e2/e4=cer) or e6 separately under the control of the keratin14 promoter spontaneously developed papillomas characterized by varying degrees of epidermal dysplasia. papilloma growth could be synchronized by a single uva/b irradiation of the skin, which led to the development of papillomas within three weeks. | 2011 | 21458245 |
| genomic characterization of ten novel cutaneous human papillomaviruses from keratotic lesions of immunosuppressed patients. | viral warts from immunosuppressed organ transplant recipients (otr) persist over years and may progress into non-melanoma skin cancer. the types of human papillomaviruses (hpv) in such lesions are different from that seen in the general population. a subset of these lesions is not infected with the classical wart-associated hpv types. in order to get a better understanding of the hpv types in those lesions, we isolated ten novel hpv from atypical persisting keratotic lesions of immunosuppressed ... | 2011 | 21471318 |
| transcriptome sequencing demonstrates that human papillomavirus is not active in cutaneous squamous cell carcinoma. | β-human papillomavirus (β-hpv) dna is present in some cutaneous squamous cell carcinomas (cusccs), but no mechanism of carcinogenesis has been determined. we used ultra-high-throughput sequencing of the cancer transcriptome to assess whether papillomavirus transcripts are present in these cancers. in all, 67 cuscc samples were assayed for β-hpv dna by pcr, and viral loads were measured with type-specific quantitative pcr. a total of 31 sccs were selected for whole transcriptome sequencing. trans ... | 2011 | 21490616 |
| the association between cutaneous squamous cell carcinoma and betapapillomavirus seropositivity: a cohort study. | background: it is currently unclear whether betapapillomaviruses (betapv) play a role in the etiology of cutaneous squamous cell carcinoma (scc). we investigated the association between betapv antibodies and subsequent scc in a population-based cohort study. methods: serum samples were collected in 1992 and/or 1996 from 1,311 participants of the community-based nambour skin cancer study. these were tested for the presence of l1 antibodies against 21 different betapv types. histologically diagnos ... | 2011 | 21527580 |
| characterization of novel cutaneous human papillomavirus genotypes hpv-150 and hpv-151. | dna from two novel hpv genotypes, hpv-150 and hpv-151, isolated from hair follicles of immuno-competent individuals, was fully cloned, sequenced and characterized. the complete genomes of hpv-150 and hpv-151 are 7,436-bp and 7,386-bp in length, respectively. both contain genes for at least six proteins, namely e6, e7, e1, e2, l2, l1, as well as a non-coding upstream regulatory region located between the l1 and e6 genes: spanning 416-bp in hpv-150 (genomic positions 7,371 to 350) and 322-bp in hp ... | 2011 | 21799888 |
| a case-control study of betapapillomavirus infection and cutaneous squamous cell carcinoma in organ transplant recipients. | we examined the association between betapapillomavirus (betapv) infection and cutaneous squamous cell carcinoma (scc) in organ transplant recipients. a total of 210 organ transplant recipients with previous scc and 394 controls without skin cancer were included. the presence of 25 betapv types in plucked eyebrow hairs was determined using a human papillomavirus (hpv) dna genotyping assay, and antibodies for the 15 most prevalent betapv types were detected using multiplex serology. we used multiv ... | 2011 | 21718442 |
| the oral cavity contains abundant known and novel human papillomaviruses from the betapapillomavirus and gammapapillomavirus genera. | background.ôçâhuman papillomaviruses (hpvs) primarily sort into 3 genera: alphapapillomavirus (+¦-hpv), predominantly isolated from mucosa, and betapapillomavirus (+¦-hpv) and gammapapillomavirus (+¦-hpv), predominantly isolated from skin. hpv types might infect body sites that are different from those from which they were originally isolated. methods.ôçâwe investigated the spectrum of hpv type distribution in oral rinse samples from 2 populations: 52 human immunodeficiency virus (hiv)-positive ... | 2011 | 21844305 |
| human papillomavirus 8 e6 disrupts terminal skin differentiation and prevents pro-caspase-14 cleavage. | expression of the betapapillomavirus (betapv) e6/e7 genes has been shown to impair both keratinocyte differentiation and apoptosis. especially late-terminal keratinocyte differentiation shares certain aspects with apoptosis, such as fragmentation of dna and activation of caspases. here we investigated the disruption of keratinocyte differentiation in organotypic skin (raft) cultures of primary (phk) and immortalized (n/tert) human keratinocytes, in particular by human papillomavirus (hpv)8. immu ... | 2011 | 22230316 |