| identification and characterization of the gene encoding the acidobacterium capsulatum major sigma factor. | acidobacterium capsulatum is an acid-tolerant, encapsulated, gram-negative member of the ubiquitous, but poorly understood acidobacteria phylum. little is known about the genetics and regulatory mechanisms of a. capsulatum. to begin to address this gap, we identified the gene encoding the a. capsulatum major sigma factor, rpod, which encodes a 597-amino acid protein with a predicted sequence highly similar to the major sigma factors of solibacter usitatus ellin6076 and geobacter sulfurreducens p ... | 2006 | 16698197 |
| expanding the family of collagen proteins: recombinant bacterial collagens of varying composition form triple-helices of similar stability. | the presence of the (gly-xaa-yaa)(n) open reading frames in different bacteria predicts the existence of an expanded family of collagen-like proteins. to further explore the triple-helix motif and stabilization mechanisms in the absence of hydroxyproline (hyp), predicted novel collagen-like proteins from gram-positive and -negative bacteria were expressed in escherichia coli and characterized. soluble proteins capable of successful folding and in vitro refolding were observed for collagen protei ... | 2010 | 20025291 |
| biological consequences of ancient gene acquisition and duplication in the large genome of candidatus solibacter usitatus ellin6076. | members of the bacterial phylum acidobacteria are widespread in soils and sediments worldwide, and are abundant in many soils. acidobacteria are challenging to culture in vitro, and many basic features of their biology and functional roles in the soil have not been determined. candidatus solibacter usitatus strain ellin6076 has a 9.9 mb genome that is approximately 2-5 times as large as the other sequenced acidobacteria genomes. bacterial genome sizes typically range from 0.5 to 10 mb and are in ... | 2011 | 21949776 |