| inhibition of rna synthesis in chlorella pyrenoidosa and bacillus megaterium by the pine-blight toxin, dothistromin. | dosthistromin, an anthraquinone derivative produced by the pine-blight fungus, dothistroma pini, inhibits the growth of chlorella pyrenoidosa and bacillus megaterium. at growth inhibitory concentrations, dothistromin strongly inhibits incorporation of [3h]uridine into rna of both species. with b. megaterium, marked inhibition of [3h]uridine incorporation is apparent within 5 min of addition of dothistromin, but only a slight inhibition of [3h]thymidine incorporation into the dna-containing fract ... | 1976 | 822126 |
| dothistroma pini, a forest pathogen, contains homologs of aflatoxin biosynthetic pathway genes. | homologs of aflatoxin biosynthetic genes have been identified in the pine needle pathogen dothistroma pini. d. pini produces dothistromin, a difuranoanthraquinone toxin with structural similarity to the aflatoxin precursor versicolorin b. previous studies with purified dothistromin suggest a possible role for this toxin in pathogenicity. by using an aflatoxin gene as a hybridization probe, a genomic d. pini clone was identified that contained four dot genes with similarity to genes in aflatoxin ... | 2002 | 12039746 |
| sieve element specific labeling with an anti-idiotypic monoclonal antibody mimic of the phytotoxin dothistromin. | a monoclonal antibody, 12c9, an anti-idiotypic mimic of dothistromin, a toxin produced by dothistroma pini, was found to label the cell wall of sieve elements in a number of different plant tissues and species. the antibody labeled apple leaf tissue, tobacco leaf mid vein, leaf and meristem, and coprosma robusta leaf mid vein. labeling was restricted to cell walls of sieve elements and did not label the companion cells or the lumen of the cells. the antibody labeled over a wide range of dilution ... | 2003 | 14533843 |
| development, comparison, and validation of real-time and conventional pcr tools for the detection of the fungal pathogens causing brown spot and red band needle blights of pine. | dothistroma pini, d. septosporum, and lecanosticta acicola are fungal pathogens that cause severe foliage diseases in conifers. all three pathogens are listed as quarantine organisms in numerous countries throughout the world and, thus, are subject to specific monitoring. detection and identification of these pathogens still often relies on cumbersome and unsatisfactory methods that are based upon the morphological characterization of fungal fruiting bodies and conidia. in this study, we present ... | 2010 | 19968556 |
| microsatellite markers for the red band needle blight pathogen, dothistroma septosporum. | twelve microsatellite markers were developed for population analyses of the fungal pathogen, dothistroma septosporum. intersimple sequence repeat polymerase chain reaction (issr-pcr) and an enrichment protocol (fast isolation by amplified fragment length polymorphism of sequences containing repeats [fiasco]) were both used to identify 28 unique microsatellite regions in the genome. from 22 primer pairs designed, 12 were polymorphic. these markers, screened on two populations representing 42 isol ... | 2008 | 21585961 |
| is the emergence of dothistroma needle blight of pine in france caused by the cryptic species dothistroma pini? | dothistroma needle blight (dnb) emerged in france in the past 15 years. this disease is induced by two closely related species: dothistroma septosporum and d. pini. although both species are currently present in france, only d. septosporum was reported in the past. we investigated whether a recent arrival of d. pini in france could be a cause of the dnb emergence. we analyzed herbarium specimens of pine needles with dnb symptoms using polymerase chain reaction techniques to study the past freque ... | 2012 | 22165983 |