| heteroduplex mobility assay detects dna mutations for differentiation of closely related phytoplasma strains. | we present the first use of dna heteroduplex mobility assay (hma) to detect the point mutations including substitutions and deletions/insertions in 16s rdna of aster yellows phytoplasma (ay27) and to differentiate phytoplasmas collected from field samples of clover proliferation (cp) and alfalfa witches'-broom (awb). the phytoplasmal 16s rdna fragment was amplified from ay27 by polymerase chain reaction (pcr) and cloned into a plasmid vector. the cloned dna fragment was subjected to in vitro mut ... | 2000 | 10856778 |