| analysis of extracellular proteins of two perkinsus spp. isolated from the softshell clam mya arenaria in vitro. | biochemical characterization of the extracellular proteins (ecp) of two softshell clam perkinsus spp. cloned isolates, perkinsus chesapeaki isolate g-117 and perkinsus marinus h-49, was performed and compared to that of the oyster-derived p. marinus isolate p-1. g-117 and h-49 demonstrated distinct differences in enzyme activities; however, all three isolates shared common bands. substrate-impregnated gels showed h-49 to possess proteolytic activities while g-117 did not. inhibition studies reve ... | 2000 | 11026671 |
| description of perkinsus andrewsi n. sp. isolated from the baltic clam (macoma balthica) by characterization of the ribosomal rna locus, and development of a species-specific pcr-based diagnostic assay. | a perkinsus species was isolated from the baltic clam macoma balthica and an in vitro culture established under conditions described for p. marinus. as reported previously, morphological features remarkable enough to clearly indicate that this isolate is a distinct perkinsus species were lacking. in this study, regions of the rrna locus (nts, 18s, its1, 5.8s, and its2) of this isolate were cloned, sequenced, and compared by alignment with those available for other perkinsus species and isolates. ... | 2001 | 11249193 |
| molecular, morphological, and experimental evidence support the synonymy of perkinsus chesapeaki and perkinsus andrewsi. | diverse analytical and experimental results confirm that two protistan parasites, perkinsus chesapeaki and perkinsus andrewsi, described separately as parasites of mya arenaria and macoma balthica clams sympatric in chesapeake bay, usa, represent a single species. ribosomal rna (rrna) internal transcribed spacer (its) regions, rrna large subunit (lsu) gene, and actin gene sequences were obtained from clonal perkinsus spp. cultured in vitro. although multiple polymorphic sequences were found in d ... | 2005 | 15927003 |
| assessment of the northern distribution range of selected perkinsus species in eastern oysters (crassostrea virginica) and hard clams (mercenaria mercenaria) with the use of pcr-based detection assays. | perkinsus species are protistan parasites of molluscs. in chesapeake bay, perkinsus marinus, perkinsus chesapeaki, and perkinsus andrewsi are sympatric, infecting oysters and clams. although p. marinus is a pathogen for crassostrea virginica, it remains unknown whether p. andrewsi and p. chesapeaki are equally pathogenic. perkinsus species have been reported in c. virginica as far north as maine, sometimes associated with high prevalence, but low mortality. thus, we hypothesized that, in additio ... | 2008 | 18564742 |
| survey for protozoan parasites in eastern oysters (crassostrea virginica) from the gulf of maine using pcr-based assays. | protozoan pathogens represent a serious threat to oyster aquaculture, since they can lead to significant production loses. moreover, oysters can concentrate human pathogens through filter feeding, thus putting at risk raw oyster consumers' health. using pcr-based assays in oysters (crassostrea virginica) from maine, we expand the northeast range in the usa for the protozoans perkinsus marinus, perkinsus chesapeaki, and haplosporidium nelsoni, and report for the first time the detection of the hu ... | 2015 | 25889457 |
| first record of perkinsus chesapeaki infecting crassostrea rhizophorae in south america. | this study investigated perkinsus spp. infecting crassostrea rhizophorae from the jaguaribe river estuary, ceará, brazil. fragments of gills and rectum of the oysters (n=150) were incubated in ray's fluid thioglycollate medium (rftm). genus perkinsus-specific perkits85/750 pcr assays were performed and their amplicons were sequenced by the sanger method. the rftm assays confirmed perkinsus spp. the sequencing of the amplified fragments from the rdna internal transcribed spacers (its) of perkinsu ... | 2016 | 27810288 |
| richness and distribution of tropical oyster parasites in two oceans. | parasites can exert strong effects on population to ecosystem level processes, but data on parasites are limited for many global regions, especially tropical marine systems. characterizing parasite diversity and distributions are the first steps towards understanding the potential impacts of parasites. the panama canal serves as an interesting location to examine tropical parasite diversity and distribution, as it is a conduit between two oceans and a hub for international trade. we examined met ... | 2016 | 27263626 |
| perkinsus olseni and p. chesapeaki detected in a survey of perkinsosis of various clam species in galicia (nw spain) using pcr-dgge as a screening tool. | a survey on perkinsosis was performed involving 15 locations scattered along the galician coast (nw spain) and four clam species with high market value (ruditapes decussatus, ruditapes philippinarum, venerupis corrugata and polititapes rhomboides). the prevalence of perkinsus parasites was estimated by pcr using genus-specific primers. the highest percentage of pcr-positive cases for perkinsosis corresponded to clams r. decussatus and v. corrugata, while lower values were detected in r. philippi ... | 2016 | 26628210 |
| galectin cvgal2 from the eastern oyster (crassostrea virginica) displays unique specificity for abh blood group oligosaccharides and differentially recognizes sympatric perkinsus species. | galectins are highly conserved lectins that are key to multiple biological functions, including pathogen recognition and regulation of immune responses. we previously reported that cvgal1, a galectin expressed in phagocytic cells (hemocytes) of the eastern oyster (crassostrea virginica), is hijacked by the parasite perkinsus marinus to enter the host, where it causes systemic infection and death. screening of an oyster hemocyte cdna library revealed a novel galectin, which we designated cvgal2, ... | 2015 | 26158802 |
| comparison of protein expression profiles between three perkinsus spp., protozoan parasites of molluscs, through 2d electrophoresis and mass spectrometry. | the genus perkinsus includes protozoan parasites of a wide range of marine molluscs worldwide, some of which have been responsible for heavy mollusc mortalities and dramatic economic losses. this study was performed with the aim of increasing the knowledge of perkinsus spp. proteome. proteins extracted from in vitro cultured cells of three species of this genus, p. marinus, p. olseni and p. chesapeaki, were analysed using 2d electrophoresis. four gels from each species were produced. qualitative ... | 2014 | 24607654 |
| perkinsus chesapeaki observed in a new host, the european common edible cockle cerastoderma edule, in the spanish mediterranean coast. | histological observations showed the presence of a perkinsus sp. parasite in cerastoderma edule tissues for the first time in the spanish mediterranean coast. its molecular characterization by pcr-rflp, in situ hybridization and sequencing, identified the parasite as perkinsus chesapeaki, with a maximum identity of 99-100% with genbank p. chesapeaki sequences from france and 97% with p. chesapeaki sequences of north american origin when blast analysis was carried out. furthermore, phylogenetic s ... | 2014 | 24525498 |
| perkinsus chesapeaki in stout razor clams tagelus plebeius from delaware bay. | perkinsus chesapeaki is reported from stout razor clams tagelus plebeius in delaware bay, extending the known range of p. chesapeaki north of chesapeake bay. p. marinus, which causes dermo disease, is prevalent in cultured and wild oysters at this site, but was not detected in t. plebeius. evidence for the presence of disseminated neoplasia, also reported from chesapeake bay, was equivocal. although p. chesapeaki infections were associated with mortality events, light infection intensities and a ... | 2008 | 18380223 |
| identification of a second rrna gene unit in the perkinsus andrewsi genome. | perkinsus species are parasitic protozoa of mollusks, currently classified within the perkinsozoa, a recently established phylum that is basal to the apicomplexa and dinozoa. ribosomal rna (rrna) genes and their intergenic spacers have been used to support the taxonomy of perkinsus species, the description of new species, and to develop molecular probes for their detection and identification. we previously described ultrastructure, behavior in culture, and partial sequence of the rrna locus of a ... | 2009 | 15134261 |