| cellular identification of a novel uncultured marine stramenopile (mast-12 clade) small-subunit rrna gene sequence from a norwegian estuary by use of fluorescence in situ hybridization-scanning electron microscopy. | revealing the cellular identity of organisms behind environmental eukaryote rrna gene sequences is a major objective in microbial diversity research. we sampled an estuarine oxygen-depleted microbial mat in southwestern norway and retrieved an 18s rrna gene signature that branches in the mast-12 clade, an environmental marine stramenopile clade. detailed phylogenetic analyses revealed that mast-12 branches among the heterotrophic stramenopiles as a sister of the free-living bicosoecida and the p ... | 2007 | 17293516 |
| grazing rates and functional diversity of uncultured heterotrophic flagellates. | aquatic assemblages of heterotrophic protists are very diverse and formed primarily by organisms that remain uncultured. thus, a critical issue is assigning a functional role to this unknown biota. here we measured grazing rates of uncultured protists in natural assemblages (detected by fluorescent in situ hybridization (fish)), and investigated their prey preference over several bacterial tracers in short-term ingestion experiments. these included fluorescently labeled bacteria (flb) and two st ... | 2009 | 19129862 |
| solenicola setigera is the first characterized member of the abundant and cosmopolitan uncultured marine stramenopile group mast-3. | culture-independent molecular methods based on the amplification, cloning and sequencing of small-subunit (ssu) rrna genes are a powerful tool to study the diversity of prokaryotic and eukaryotic microorganisms for which morphological features are not conspicuous. in recent years, molecular data from environmental surveys have revealed several clades of protists lacking cultured and/or described members. among them are various clades of marine stramenopiles (heterokonts), which are thought to pl ... | 2011 | 20722698 |