| characterization of minute virus of canines (mvc) and its pathogenicity for pups. | minute virus of canines (mvc, canine parvovirus-1), originally isolated in 1970 from the feces of normal dogs, was compared with canine parvovirus type-2 (cpv-2). the two viruses, which differ in their host cell ranges and spectra of hemagglutination, also were found distinct in their antigenic and genomic properties. we demonstrated that the mvc replicates in dogs and is capable of producing pathologic changes that were most prominent in oronasally-exposed neonatal pups. macroscopic and microsc ... | 1988 | 2836128 |
| hemagglutination by canine parvovirus: serologic studies and diagnostic applications. | conditions for canine parvoviral hemagglutination (ha) and hemagglutination-inhibition (hi) reactions were defined. the ha phenomena were used to differentiate canine parvovirus (cpv) from feline panleukopenia virus (fpv), mink enteritis virus (mev), and minute virus of canines. serologic comparisons of the cpv, fpv, and mev by ha-hi and serum-neutralization tests indicated that cpv, fpv, and mev were antigenically similar but were different from minute virus of canines. diagnostic application o ... | 1980 | 6250432 |
| establishment of a canine cell line: derivation, characterization, and viral spectrum. | a cell line, designated a-72, for virus studies was established from a tumor surgically removed from a female, 8-year-old golden retriever dog. following explant culture, the cells were serially passaged 135 times. the a-72 cells maintained a fibroblastic appearance and, at the 123rd passage, had a population doubling time of approximately 27 hours. karyotypic analysis of the high passage cells showed the modal 2n chromosome number was 92 to 93. using starch gel electrophoresis for enzyme charac ... | 1980 | 6254399 |
| viral antibody studies of laboratory dogs with diarrheal disease. | viral antibody studies were done on laboratory dogs in an epizootic of gastrointestinal disease. increased hemagglutination-inhibition antibody titers to a parvovirus (pv) antigenically related to feline panleukopenia virus were found in convalescent serum specimens of 78% (20/26) of the affected dogs and in 83% (5/6) of apparently healthy dogs. with one exception, all dogs tested had significant levels of hemagglutination-inhibition antibody to this pv. similar increased antibody titers were fo ... | 1981 | 6275746 |
| a fatal case of pup infection with minute virus of canines (mvc). | | 1996 | 8953537 |
| a serological survey of minute virus of canines (mvc; canine parvovirus type-1) in dogs in the tokai area of japan. | a serological survey for antibodies to minute virus of canines (mvc) by use of a hemagglutination-inhibition (hi) test was performed on sera collected from dogs in the tokai area of japan. forty-one of 266 (15.4%) sera had positive titers of 1:40 or higher against the mvc. results suggest that mvc may have been present in dogs in japan since, at least, 1990. from this serosurvey, mvc appears to be established in the dog population in japan. mvc may have a role as a newly recognized viral pathoge ... | 2001 | 11799909 |
| virologic and serologic identification of minute virus of canines (canine parvovirus type 1) from dogs in japan. | minute virus of canines (mvc), also known as canine parvovirus type 1, was initially believed to be a nonpathogenic agent, since it was first isolated from canine fecal specimens in the late 1960s. however, subsequent pathological as well as epidemiological studies suggested that mvc is a pathogen of neonatal puppies and is widely distributed among domestic dogs in the united states. the virus also has been shown to cause fetal deaths. nevertheless, the virus was not detected in dogs outside the ... | 2002 | 12409364 |
| the canine minute virus (minute virus of canines) is a distinct parvovirus that is most similar to bovine parvovirus. | we characterized the genome and proteins of the canine minute virus (the minute virus of canines (mvc)). the genome sequence showed mvc to be an autonomous parvovirus encoding a large nonstructural protein 1 gene, a smaller nonstructural protein, and overlapping vp1 and vp2 protein genes. the virus was most closely related to bovine parvovirus (bpv), with which it was 43% identical at the dna sequence level, while the ns1 and vp1 proteins were 33.6 and 41.4% identical to those of bpv, respective ... | 2002 | 12441065 |
| seroprevalence of canine calicivirus and canine minute virus in the republic of korea. | | 2003 | 12934799 |
| real-time pcr protocol for the detection of porcine parvovirus in field samples. | this report describes a real-time polymerase chain reaction assay with sybr green for detection of a broad range of porcine parvoviruses (ppv) and accurate virus quantification in porcine tissues. the assay targets the vp2 gene of ppv and the porcine genomic c-myc gene for normalization. the detection limit of the sybr green reaction was shown to be equivalent to 6 x 10(0) to 6 x 10(1) ppv copies/reaction and the overall detection limit equivalent to 0.1 tcid(50)/100 microl. the assay was linear ... | 2006 | 16472875 |
| recovery and characterization of a minute virus of canines. | four antigenically related transmissible agents were recovered from canine fecal specimens. the agents produced cytopathic effects in a continuous dog cell line developed in this laboratory. increased antibody titers were demonstrated in three of the four dogs which provided the isolates. the virus did not produce cytopathic effects in primary canine kidney or thymus cell cultures, or in cell cultures of human, simian, porcine, bovine, feline, and murine origin. the agent is resistant to ether, ... | 1970 | 16557766 |
| etiologic study of upper respiratory infections of household dogs. | infectious tracheobronchitis (itb), also known as the kennel cough, is a respiratory syndrome of dogs and usually appears to be contagious among dogs housed in groups. etiologic agent of itb is multiple and sometimes complex. in the present study, 68 household dogs showing clinical signs of respiratory infection were examined, and 20 dogs (29.4%) were found to be positive for either of following agents. bordetella bronchiseptica (b.b.) was most frequently detected from nasal and oropharynx sites ... | 2008 | 18628596 |
| animal bocaviruses: a brief review. | the recently discovered human bocavirus has been preliminary classified into the genus bocavirus of the family parvoviridae. animal bocaviruses have been known in veterinary medicine since the early 1960s. this paper reviews the current knowledge about the two eponymous members of the genus: bovine parvovirus (bpv) and canine minute virus (cnmv). in contrast to other parvoviruses, bocaviruses contain a third open reading frame in the mid of the genome encoding for a highly phosphorylated non-str ... | 2008 | 19023216 |
| sequence analysis of an isolate of minute virus of canines in china reveals the closed association with bocavirus. | in the present study, we have cloned and sequenced the nearly-full-length genome of minute virus of canines (mvc), sh26, in china. the genome of mvc, 5,132 nucleotides (nts) in length, contains three open reading frames (orfs), which are 2,325-bp of ns1, 561-bp of np1 and 2,112-bp of vp1/vp2 encoding three proteins of 774, 186 and 703 residues, respectively. predicted amino acids sequence of ns1 of mvc has 44% identity with human bocavirus (hbov) and human boacvirus 2 (hbov2), np1 has 48 and 45% ... | 2010 | 19760094 |
| bocavirus infection induces mitochondrion-mediated apoptosis and cell cycle arrest at g2/m phase. | bocavirus is a newly classified genus of the family parvovirinae. infection with bocavirus minute virus of canines (mvc) produces a strong cytopathic effect in permissive walter reed/3873d (wrd) canine cells. we have systematically characterized the mvc infection-produced cytopathic effect in wrd cells, namely, the cell death and cell cycle arrest, and carefully examined how mvc infection induces the cytopathic effect. we found that mvc infection induces an apoptotic cell death characterized by ... | 2010 | 20335259 |
| human bocavirus capsid structure: insights into the structural repertoire of the parvoviridae. | human bocavirus (hbov) was recently discovered and classified in the bocavirus genus (family parvoviridae, subfamily parvovirinae) on the basis of genomic similarity to bovine parvovirus and canine minute virus. hbov has been implicated in respiratory tract infections and gastroenteric disease in children worldwide, yet despite numerous epidemiological reports, there has been limited biochemical and molecular characterization of the virus. reported here is the three-dimensional structure of reco ... | 2010 | 20375175 |
| development and validation of a real-time pcr assay for specific and sensitive detection of canid herpesvirus 1. | a taqman-based real-time pcr assay targeting the glycoprotein b-encoding gene was developed for diagnosis of canid herpesvirus 1 (chv-1) infection. the established assay was highly specific, since no cross-reactions were observed with other canine dna viruses, including canine parvovirus type 2, canine minute virus, or canine adenovirus types 1 and 2. the detection limit was 10(1) and 1.20 x 10(1) dna copies per 10 microl(-1) of template for standard dna and a chv-1-positive kidney sample, respe ... | 2010 | 20674611 |
| complete coding sequences and phylogenetic analysis of porcine bocavirus. | here we report, for the first time, the nearly full-length genome sequence of porcine bocavirus (pbov), a recently discovered parvovirus from pigs. phylogenetic trees based on this genome sequence showed that pbov belongs to the branch containing the genus bocavirus, which comprises canine minute virus (cnmv), bovine parvovirus, gorilla bocavirus and human bocavirus (hbov), and was most closely related to the group containing cnmv. pbov was predicted to contain three potential orfs encoding the ... | 2011 | 21228124 |
| human bocavirus--a novel parvovirus to infect humans. | for almost three decades parvovirus b19 has been described as the only member of the parvoviridae to infect and cause illness in humans. this statement was correct until 2005 when a group of swedish scientists identified a previously uncharacterized virus in pools of human nasopharyngeal aspirates obtained from individuals suffering from diseases of the respiratory tract. comprehensive sequence and phylogenetic analysis allowed the identification of the new virus as a member of the parvoviridae. ... | 2008 | 18536522 |
| parvovirus infections in wild carnivores. | various parvoviruses infect carnivores and can cause disease. in this review article the knowledge about infections of free-ranging or captive carnivores with the feline parvoviruses, feline panleukopenia virus, and canine parvovirus, including the antigenic types cpv-2a and -2b, as well as aleutian disease of mink virus and minute virus of canines are summarized. particular emphasis is placed on description of the evolution of canine parvovirus which apparently involved wild carnivore hosts. | 2001 | 11504234 |
| [the "other" parvovirus: first description of the minute virus of canines (canine parvovirus type 1) in germany]. | during an investigation of a late abortion in a yorkshire terrier minute virus of canines (mvc) antigen could be demonstrated in fetal tissues by immunofluorescence. the bitch had a high specific anti-mvc serum titer when tested about four weeks after the abortion. those results implicate an etiological role of mvc in this abortion and to our knowledge it presents the first described case of a mvc-associated abortion in germany. | 1996 | 8999601 |
| minute virus of canines (mvc, canine parvovirus type-1): pathogenicity for pups and seroprevalence estimate. | minute virus of canines (mvc, canine parvovirus type-1) caused inapparent to severe illness in neonatal specific-pathogen-free pups exposed by the oronasal route. the experimental disease was generally mild. four of 21 infected pups had clinical signs of respiratory illness, but only 2 pups, not euthanized during the early postinoculation period, developed severe illness or died. principal pathologic changes included bronchitis and interstitial pneumonia with various degrees of lymphadenitis. in ... | 1994 | 8068747 |
| pathogenicity of minute virus of canines (mvc) for the canine fetus. | minute virus of canines (mvc) was shown to cause transplacental infections with embryo resorptions, especially in dams given parenteral inoculations prior to gestational day 30. responses to oral-nasal inocula were inconsistent. direct exposures of embryos or fetuses to mvc via the amniotic sac, however, resulted in their deaths after an incubation period of approximately 2 weeks. virus was prominent in fetal lung and small intestinal villi, but it could not be demonstrated in fetal or uterine t ... | 1991 | 1851474 |
| fatal disease in nursing puppies associated with minute virus of canines. | thirteen cases of a previously undescribed parvoviral infection affecting puppies ranging in age from 5 to 21 days is described. the cases were originally thought to represent an unusual pathologic manifestation of canine parvovirus-2 (cpv-2) infection. however, failure to confirm cpv-2 infection in any of the cases suggested a different parvovirus was involved. minute virus of canines (mvc) was subsequently isolated from a case by using the walter reed canine cell line, the only cell line which ... | 1992 | 1313305 |
| canine hemorrhagic enteritis: detection of viral particles by electron microscopy. | at necropsy, several dogs which died showing symptoms of hemorrhagic diarrhea, had significant lesions of the mucosa that were found especially in the duodenum and upper part of the small bowel. study of ultrathin sections from the diseased mucosa revealed particles resembling parvoviruses in altered nuclei of cells of the intestinal crypts. electron microscopic examination of intestinal contents by negative staining has shown the presence of many viral particles which have a diameter of 24 nm a ... | 1979 | 316320 |
| molecular characteristics of a novel strain of canine minute virus associated with hepatitis in a dog. | a 5-year-old female yorkshire terrier dog died a few days following hernia and ovariohysterectomy surgeries. necropsy performed on the dog revealed that the surgeries were not the cause of death; however, degenerative viral hepatitis, showing intranuclear inclusion bodies in hepatic cells, was observed in histopathologic examination. several diagnostic methods were used to screen for the cause of disease, and minute virus of canines (mvc) was detected in all parenchymal organs, including the liv ... | 2016 | 27251050 |
| the minute virus of canines (mvc) np1 protein governs the expression of a subset of essential ns proteins via its role in rna processing. | parvoviruses use a variety of means to control the expression of their compact genomes. the bocaparvovirus minute virus of canines (mvc) encodes a small, genus-specific protein, np1, which governs access to the viral capsid gene via its role in alternative polyadenylation and alternative splicing of the single mvc pre-mrna. in addition to np1, mvc encodes five additional non-structural proteins (ns) that share an initiation codon at the left end of the genome and which are individually encoded b ... | 2017 | 28356522 |
| characterization of novel canine bocaviruses and their association with respiratory disease. | we report the first identification, genetic characterization and disease association studies of several novel species of canine bocaviruses (cbov). evolutionary analysis confirmed that cbov are genetically distinct from the only other known canine bocavirus, minute virus of canines (mvc), with which they share less than 63, 62 and 64% protein identity in ns, np and vp genes, respectively. comparative genetic analysis of 37 vp gene variants found in diseased and healthy animals showed that these ... | 2011 | 22031527 |
| identification and nearly full-length genome characterization of novel porcine bocaviruses. | the genus bocavirus includes bovine parvovirus (bpv), minute virus of canines (mvc), and a group of human bocaviruses (hbov1-4). using sequence-independent single primer amplification (sispa), a novel bocavirus group was discovered with high prevalence (12.59%) in piglet stool samples. two nearly full-length genome sequences were obtained, which were approximately 5,100 nucleotides in length. multiple alignments revealed that they share 28.7-56.8% dna sequence identity with other members of parv ... | 2010 | 21049037 |
| bocavirus infection induces a dna damage response that facilitates viral dna replication and mediates cell death. | minute virus of canines (mvc) is an autonomous parvovirus that replicates efficiently without helper viruses in walter reed/3873d (wrd) canine cells. we previously showed that mvc infection induces mitochondrion-mediated apoptosis and g(2)/m-phase arrest in infected wrd cells. however, the mechanism responsible for these effects has not been established. here, we report that mvc infection triggers a dna damage response in infected cells, as evident from phosphorylation of h2ax and rpa32. we disc ... | 2010 | 21047968 |
| characterization of dog serum virome from northeastern brazil. | domestic dogs share habitats with human, a fact that makes them a potential source of zoonotic viruses. moreover, knowledge regarding possible bloodborne pathogens is important due to the increasing application of blood transfusion in dogs. in the present study, we evaluated the serum virome of 520 dogs using throughput sequencing (hts). the serum samples were pooled and sequenced using an illumina miseq platform. our unbiased method identified prevalent canine pathogens as canine protoparvoviru ... | 2018 | 30292963 |
| identification and characterization of a new bocavirus species in gorillas. | a novel parvovirus, provisionally named gorilla bocavirus species 1 (gbov1), was identified in four stool samples from western gorillas (gorilla gorilla) with acute enteritis. the complete genomic sequence of the new parvovirus revealed three open reading frames (orfs) with an organization similar to that of known bocaviruses. phylogenetic analysis using complete capsid and non structural (ns) gene sequence suggested that the new parvovirus is most closely related to human bocaviruses (hbov). ho ... | 2010 | 20668709 |
| characterization of the gene expression profile of human bocavirus. | we have generated a quantitative transcription profile of human bocavirus type 1 (hbov1) by transfecting a nearly full-length clone in human lung epithelial a549 cells as well as in a replication competent system in 293 cells. the overall transcription profile of hbov1 is similar to that of two other members of genus bocavirus, minute virus of canines and bovine parvovirus 1. in particular, a spliced ns1-transcript that was not recognized previously expressed the large non-structural protein ns1 ... | 2010 | 20457462 |
| a minute virus of canines (mvc: canine bocavirus) isolated from an elderly dog with severe gastroenteritis, and phylogenetic analysis of mvc strains. | two of the three adult dogs kept in a family developed severe gastroenteritis. from the feces of one of the affected dogs a minute virus of canines (mvc) was detected by pcr and virus isolation. that this virus had recently infected the dogs was indicated by high anti-mvc antibody titers of their sera. no other virus commonly associated with canine gastrointestinal disease was implicated. as no previous association of mvc infection and disease in aged dogs had been described, further characteriz ... | 2010 | 20427134 |
| identification and molecular cloning of a novel porcine parvovirus. | a novel porcine parvovirus, ppv4, was identified in the lung lavage of a diseased pig coinfected with porcine circovirus type 2. this virus exhibits limited similarity to its closest relative, bovine parvovirus 2, but resembles viruses of the genus bocavirus (bovine parvovirus, canine minute virus and human bocavirus) that encode an additional orf3. the orf3 of ppv4 is predicted to encode a protein of 204 amino acid residues, which is similar in size to the orf3-encoded proteins of the bocavirus ... | 2010 | 20339886 |
| absence of human bocavirus from deceased fetuses and their mothers. | the human bocavirus (hbov), a newly discovered parvovirus, is closely related to the bovine parvovirus and the canine minute virus, which are known to cause adverse pregnancy outcomes. another human parvovirus, b19, can lead to fetal hydrops, miscarriage and intrauterine fetal death (iufd). | 2010 | 20031484 |
| human bocavirus can be cultured in differentiated human airway epithelial cells. | in 2005, a human bocavirus was discovered in children with respiratory tract illnesses. attempts to culture this virus on conventional cell lines has failed thus far. we investigated whether the virus can replicate on pseudostratified human airway epithelium. this cell culture system mimics the human airway environment and facilitates culturing of various respiratory agents. the cells were inoculated with human bocavirus-positive nasopharyngeal washes from children, and virus replication was mon ... | 2009 | 19474096 |
| molecular characterization of infectious clones of the minute virus of canines reveals unique features of bocaviruses. | minute virus of canines (mvc) is a member of the genus bocavirus in the family parvoviridae. we have molecularly cloned and sequenced the 5'- and 3'-terminal palindromes of mvc. the mvc genome, 5,404 nucleotides (nt) in length, shared an identity of 52.6% and 52.1% with that of human bocavirus and bovine parvovirus, respectively. it had distinct palindromic hairpins of 183 nt and 198 nt at the left-end and right-end termini of the genome, respectively. the left-end terminus was also found in two ... | 2009 | 19211770 |
| sequence analysis of an asian isolate of minute virus of canines (canine parvovirus type 1). | minute virus of canines (mvc), also known as canine parvovirus (cpv) type 1, is an autonomous parvovirus that infects domestic dogs worldwide and responsible for clinical problems of neonates and pregnant bitches. it was preliminary described that mvc is antigenically as well as genetically different from cpv type 2 that emerged later. however, much of mvc is still obscure since only a limited number of mvc isolates have been available for the study. mvc infections of japanese and korean dogs we ... | 2004 | 15550767 |
| np1 protein of the bocaparvovirus minute virus of canines controls access to the viral capsid genes via its role in rna processing. | minute virus of canines (mvc) is an autonomous parvovirus in the genus bocaparvovirus. it has a single promoter that generates a single pre-mrna processed via alternative splicing and alternative polyadenylation to produce at least 8 mrna transcripts. mvc contains two polyadenylation sites, one at the right-hand end of the genome, (pa)d, and another complex site, (pa)p, within the capsid-coding region. during viral infection, the mrnas must extend through (pa)p and undergo additional splicing of ... | 2015 | 26637456 |
| molecular characterization of canine minute virus associated with neonatal mortality in a litter of jack russell terrier dogs. | the molecular characterization of a strain of canine minute virus (cnmv) associated with neonatal death is reported. three newborn puppies of a litter of jack russell terrier dogs died after displaying systemic disease, whereas 2 surviving puppies showed no clinical signs with the exception of transient cardiac abnormalities that were evident by electrocardiography. necropsy of 1 dead puppy revealed severe lesions in the internal organs. a strain of canine minute virus was detected in tissue sam ... | 2012 | 22604773 |
| identification and characterization of bocaviruses in cats and dogs reveals a novel feline bocavirus and a novel genetic group of canine bocavirus. | we report the identification and genome characterization of a novel bocavirus, feline bocavirus (fbov), and novel bocaviruses closely related to canine bocavirus (cbov) strain con-161 in stray cats and dogs in hong kong, respectively. fbov was detected by pcr in 7.2, 0.3, 1.6, 2.0 and 0.8% of faecal, nasal, urine, kidney and blood samples, respectively, from 364 cats, while cbov was detected in 4.6, 5.1, 6.3 and 0.3% of faecal, nasal, urine and blood samples, respectively, from 351 dogs. three f ... | 2012 | 22495233 |
| genomic features of the human bocaviruses. | the human bocavirus (hbov) was initially discovered in 2005 as the second pathogenic member of the parvovirus family, next to the human parvovirus b19. hbov has since been shown to be extremely common worldwide and to cause a systemic infection in small children often resulting in respiratory disease. three more, presumably enteric, human bocaviruses (hbov2-4) have been identified in stool samples. parvoviruses are assumed to replicate via their genomic terminal hairpin-like structures in a so-c ... | 2012 | 22389649 |
| host range of canine minute virus in cell culture. | canine minute virus (cnmv) is a member of the bocavirus genus, together with bovine parvovirus (bpv), which emerged as a new pathogen of dogs in 1967. the genus bocavirus gained more recognition after the recent identification of human bocavirus in pooled specimens from the respiratory tract of children. the cell culture host range of cnmv appears to be restricted to the walter reed canine cell (wrcc) line, although there is a report that indicates susceptibility of the madin-darby canine kidney ... | 2012 | 22826041 |
| identification and characterization of complex dual nuclear localization signals in human bocavirus np1: identification and characterization of complex dual nuclear localization signals in human bocavirus np1. | human bocavirus (hbov), closely related to canine minute virus (mvc) and bovine parvovirus (bpv), is a new member of the bocavirus genus within the parvoviridae family. the non-structural protein np1 of hbov is a nuclear localized protein and plays an important role in dna replication as well as in the evasion of host innate immunity. in the current study, we provide the first evidence that np1 possesses a non-classical nuclear localization signal (ncnls) (amino acids 7-50). embedded within this ... | 2013 | 23388199 |
| alternative polyadenylation of human bocavirus at its 3' end is regulated by multiple elements and affects capsid expression. | alternative processing of human bocavirus (hbov) p5 promoter-transcribed rna is critical for generating the structural and nonstructural protein-encoding mrna transcripts. the regulatory mechanism by which hbov rna transcripts are polyadenylated at proximal [(pa)p] or distal [(pa)d] polyadenylation sites is still unclear. we constructed a recombinant hbov infectious clone to study the alternative polyadenylation regulation of hbov. surprisingly, in addition to the reported distal polyadenylation ... | 2017 | 27881651 |
| viral gut metagenomics of sympatric wild and domestic canids, and monitoring of viruses: insights from an endangered wolf population. | animal host-microbe interactions are a relevant concern for wildlife conservation, particularly regarding generalist pathogens, where domestic host species can play a role in the transmission of infectious agents, such as viruses, to wild animals. knowledge on viral circulation in wild host species is still scarce and can be improved by the recent advent of modern molecular approaches. we aimed to characterize the fecal virome and identify viruses of potential conservation relevance of diarrheic ... | 2017 | 28649326 |
| mutations in the c-terminus of hbov ns1 affect the function of np1. | human bocavirus 1 (hbov1) is an autonomous parvovirus in the bocaparvovirus genus. the multifunctional nuclear protein np1 is involved in viral replication. in the present study, we found that the mutations in the c-terminus of ns1 affected np1 function in viral replication. knocking out np1 expression in the recombinant infectious clone, on which the c-terminus of ns1 was mutated based on the clinical samples from nasopharyngeal aspirates, resulted in different degrees of decreased replication. ... | 2017 | 28785044 |
| the dna replication, virogenesis and infection of canine minute virus in non-permissive and permissive cells. | canine minute virus (cnmv), a kind of autonomous parvovirus, is a member of genus bocavirus in parvovirdae family. in our previous study, we constructed and obtained infectious clones of cnmv, analyzed genome characteristics, rna transcription profile, and revealed some molecular mechanisms of cytopathic effect of target cells. the purpose of this study was to investigate dna replication, virogenesis and infectious tropism of cnmv in non-permissive and permissive cells. we demonstrated that the ... | 2014 | 24239972 |
| [genomic characteristics of bocavirus and molecular mechanism of its pathogenicity--a review]. | bocavirus was considered as a member of the subfamily parvoviriae of the family parvoviridae, which includes bovine bocavirus, minute virus of canines, human bocavirus, as well as the newly identified swine bocavirus, gorilla bocavirus, feline bocavirus, canine bocavirus and bocavirus identified in california sea lion sample. at present, as an emerging pathogen, bocavirus members attract great attention by researchers worldwide. we reviewed published papers in combination with our study for seve ... | 2013 | 23957145 |
| agreement between the cell culture titrations of canine minute virus determined by two susceptibility-testing methods. | the correct diagnosis of canine minute virus is critical in dog breeding. in this study, the bland altman test was used to compare the performance of two susceptibility-testing methods, namely polymerase chain reaction (pcr) and indirect immunofluorescence assay (ifa). the agreement between ifa and pcr in monocytes revealed a mean difference of -1752.16 with 95% confidence and an interval ranging from -3229.80 to -274.53 (sd=2325.62). the agreement between ifa and pcr in walter reed canine cells ... | 2013 | 23746869 |
| parvovirus infection-induced dna damage response. | parvoviruses are a group of small dna viruses with ssdna genomes flanked by two inverted terminal structures. due to a limited genetic resource they require host cellular factors and sometimes a helper virus for efficient viral replication. recent studies have shown that parvoviruses interact with the dna damage machinery, which has a significant impact on the life cycle of the virus as well as the fate of infected cells. in addition, due to special dna structures of the viral genomes, parvoviru ... | 2013 | 25429305 |
| a novel bocavirus in canine liver. | bocaviruses are classified as a genus within the parvoviridae family of single-stranded dna viruses and are pathogenic in some mammalian species. two species have been previously reported in dogs, minute virus of canines (mvc), associated with neonatal diseases and fertility disorders; and canine bocavirus (cbov), associated with respiratory disease. | 2013 | 23402347 |
| smc1-mediated intra-s-phase arrest facilitates bocavirus dna replication. | activation of a host dna damage response (ddr) is essential for dna replication of minute virus of canines (mvc), a member of the genus bocavirus of the parvoviridae family; however, the mechanism by which ddr contributes to viral dna replication is unknown. in the current study, we demonstrate that mvc infection triggers the intra-s-phase arrest to slow down host cellular dna replication and to recruit cellular dna replication factors for viral dna replication. the intra-s-phase arrest is regul ... | 2013 | 23365434 |
| characterization of the nonstructural proteins of the bocavirus minute virus of canines. | we present a detailed characterization of a single-cycle infection of the bocavirus minute virus of canines (mvc) in canine wrd cells. this has allowed identification of an additional smaller ns protein that derives from an mrna spliced within the ns gene that had not been previously reported. in addition, we have identified a role for the viral np1 protein during infection. np1 is required for read-through of the mvc internal polyadenylation site and, thus, access of the capsid gene by mvc mrna ... | 2013 | 23135724 |