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the acid phosphatase activity and morphological characteristics of dipetalonema dracunculoides (cobbold, 1870) microfilariae.the acid phosphatase activity and some morphological characteristics of dipetalonema dracunculoides microfilariae are described. their morphological features are closely related to those of the pathogenic dirofilaria immitis when knott's technique is used for the microfilarial diagnosis. the acid phosphatase activity pattern found in dip. dracunculoides microfilariae is clearly different from those previously described for d. immitis, d. repens and dip. reconditum.19892800304
[dipetalonema dracunculoides (cobbold, 1870) (nematoda: onchocercidae) in an imported dog].dipetalonema dracunculoides was found in the abdominal and thoracic cavities of an imported dog.19883376067
parasites of stray dogs in the rabat region, morocco.eighteen parasites were identified from 57 stray dogs from the urban and rural areas of rabat. the prevalence of the species of parasites was: ctenocephalides (c. canis, c. felis) 94.7%, rhipicephalus sanguineus 68.5%, linguatula serrata, babesia canis, isospora canis each 3.5%, toxocara canis 7%, dirofilaria immitis, taenia hydatigena, t. pisiformis each 12.3%, dipetalonema dracunculoides 10.5%, ancylostoma caninum 17.5% echinococcus granulosus 22.8%, toxascaris leonina 33.3%, dipylidium caninu ...19873675044
[serologic diagnosis of filariasis by immunofluourescence on eggs of dipetalonema dracunculoides]. 19724620613
acanthocheilonema dracunculoides (cobbold, 1870) in dogs in lahore, west pakistan. 19715166315
stage-specific development of a filarial nematode (dipetalonema dracunculoides) in vector ticks.this paper reports the development of the canid filarial worm, dipetalonema dracunculoides, in the brown dog tick, rhipicephalus sanguineus by determining whether development is similar within larval, nymphal and adult stages of infected ticks. this study demonstrates that only infected nymphal ticks can support the complete development of the filarial worm. infected larval ticks are not suitable intermediate hosts, nor are infected adults. development depends on some stage-specific property of ...19947829843
experimental transmission of dipetalonema dracunculoides (cobbold 1870) by rhipicephalus sanguineus (latreille 1806).a dog naturally infected with dipetalonema dracunculoides and having a microfilaremia of 6050 microfilariae per mm3 of blood was used as source of infection. experimentally cultivated nymphs of rhipicephalus sanguineus were fed on the donor dog. once engorged, ninety-three nymphs were removed and kept at 30 degrees c and rh 90% until they moulted to the adult stage. to study the development of microfilariae in the vector, ten ticks were dissected at day 37 post-infection. adult infected ticks we ...19938333138
canine filariasis. importance and transmission in the baix llobregat area, barcelona (spain).field and laboratory studies were performed in order to assess the degree of canine dirofilariasis caused by dirofilaria immitis (leidy) in the baix llobregat region, a fluvial area near barcelona, spain. a total of 188 dogs were sampled between may and august of 1994. three main areas were chosen: the western delta, the eastern delta and the northern zone. simultaneously, a mosquito sampling programme was carried out with co2 light traps, to search for infective larvae (l3) of d. immitis. of th ...19989763317
histochemical differentiation of dirofilaria immitis, dirofilaria repens and acanthocheilonema dracunculoides microfilariae by staining with a commercial kit, leucognost-sp.the diagnosis of canine heartworm infection is based upon the presence of circulating dirofilaria immitis microfilariae or on techniques for the detection of serum antibodies or antigens. in the first of these, discrimination between d. immitis, d. repens and acanthocheilonema dracunculoides microfilariae is based upon the acid phosphatase histochemical stain. in this paper, we propose an alternative technique for histochemical staining using a commercial kit test of naphthol-as-ol (leucognost-s ...200111705664
leishmania infantum mon-98: infection in a dog from alto douro, portugal.we report the isolation of leishmania infantum zymodeme mon-98 in portugal, its first known occurrence outside the focus of el agamy, egypt. one dog found to be infected with acanthocheilonema dracunculoides during a survey of canine filariosis in the alto douro region, north-east portugal, was also discovered to have leishmania in bone marrow. the isolated strain was identified by isoenzyme analysis. a search for other possible cases of l. infantum mon-98 infection in dogs, vectors and particul ...200212062796
dipetalonema dracunculoides (nematoda: onchocercidae): first report in dog in italy. 200312847930
[presence of dipetalonema dracunculoides (cobbold, 1870) in dogs in the region of algiers]. 196013741795
prevalence of dipetalonema dracunculoides in dogs in murcia, spain. 200415214517
first record of acanthocheilonema dracunculoides from domestic dogs in namibia.acanthocheilonema dracunculoides was diagnosed in 2 dogs from windhoek, namibia, by acid phosphatase staining of microfilariae. this is the 1st record of a. dracunculoides in namibia.200617458349
hippobosca longipennis - a potential intermediate host of a species of acanthocheilonema in dogs in northern india.abstract: background: hippobosca longipennis (the 'dog louse fly') is a blood sucking ectoparasite found on wild carnivores such as cheetahs and lions and domesticated and feral dogs in africa, the middle east and asia, including china. known as an intermediate host for acanthocheilonema dracunculoides and a transport host for cheyletiella yasguri, it has also been suggested that h. longipennis may be a vector for other pathogens, including acanthocheilonema sp. ? nov., which was recently report ...201121781294
diagnosis of imported canine filarial infections in germany 2008 - 2010.filarial infections of dogs are attracting attention across europe because of the risk of spread into previously non-endemic areas (e.g. dirofilaria repens with culicidae as vectors) and as emerging zoonotic agents. the occurrence of filarial infections in german dogs has been analysed based on 8,545 samples collected either from imported animals or following travel into endemic regions. all samples were tested by means of modified knott's test and heartworm antigen assay within the period 2008 ...201121739376
occurrence of filaria in domestic dogs of samburu pastoralists in northern kenya and its associations with canine distemper.samples of blood (serum, smears and blood preserved with ethanol) were collected from dogs during a vaccination campaign in northern kenya in the years 2006 and 2007. blood was screened for filarial parasites using molecular and microscopy methods and sera were tested for antibodies against canine distemper virus (cdv). parasitological examination revealed the presence of two species of canine filariae: acanthocheilonema dracunculoides and a. reconditum. the dna from the former species was detec ...201121724332
molecular detection of leishmania infantum, filariae and wolbachia spp. in dogs from southern portugal.leishmaniosis caused by the protozoan leishmania infantum and dirofilariosis caused by the nematodes dirofilaria immitis or dirofilaria repens are vector-borne zoonoses widely present in the mediterranean basin. in addition, some studies reported that the endosymbiont wolbachia spp. play a role in the biology and pathogenesis of filarial parasites. the aim of this work was to evaluate the frequency of mono- and co-infections by l. infantum, filariae and wolbachia spp. and their association with ...201627160085
evaluation of a rapid device for serological in-clinic diagnosis of canine angiostrongylosis.angiostrongylus vasorum is a potentially fatal canine nematode. due to the high variability of clinical signs and the often chronic and subtle course of the infections, the diagnosis is particularly challenging. a rapid in-clinic assay (angio detect test, idexx laboratories, westbrook, maine, usa) for the serological detection of circulating antigen and intended for routine in-clinic diagnosis has been evaluated.201424548539
vector-borne diseases of small companion animals in namibia: literature review, knowledge gaps and opportunity for a one health approach.namibia has a rich history in veterinary health but little is known about the vector-borne diseases that affect companion dogs and cats. the aim of this review is to summarise the existing published and available unpublished literature, put it into a wider geographical context, and explore some significant knowledge gaps. to date, only two filarial pathogens (dirofilaria repens and acanthocheilonema dracunculoides) and three tick-borne pathogens (babesia canis vogeli, hepatozoon canis and ehrlic ...201526824340
nodular presentation of dirofilaria repens infection in a cat mimicking a fibrosarcoma.a cat with multiple subcutaneous nodules suggesting a soft tissue sarcoma by physical and computed tomographic examination was diagnosed as being affected by subcutaneous filariosis based on cytologic and ultrasonographic assessments. nodules were surgically removed and extracted nematodes were identified by pcr as dirofilaria repens. furthermore, dna of dipetalonema dracunculoides (syn. acantocheilonema dracunculoides) was detected by pcr, with no evidence of circulating microfilariae. to the b ...201727935633
prevalence and seasonal variations of canine dirofilariosis in portugal.dirofilariosis is a severe vector-borne emergent disease that is spreading worldwide and becoming a serious threat to human and veterinary public health. portugal, a mediterranean country, has favorable climate conditions for mosquito development and survival. at present, accurate data on the prevalence and epidemiological pattern of dirofilariosis in portugal is scarce and outdated. to study these trends, a project was developed to assess the current prevalence of dirofilaria immitis infection ...201425440945
prevalence of dirofilaria immitis (nematoda: filarioidea) in mosquitoes from northeast arkansas, the united states.a mosquito survey was conducted to identify which species of mosquitoes carry dirofilaria immitis (leidy) (nematoda: filarioidea), dog heartworm, in northeast arkansas. using polymerase chain reaction, mosquitoes were analyzed for d. immitis, dirofilaria repens railliet & henry, and acanthocheilonema dracunculoides cobbold. mosquitoes were collected from april to october 2009 using black light ultraviolet traps baited with dry ice. sixteen mosquito species were identified. d. immitis was identif ...201323926787
morphometric analyses of canine blood microfilariae isolated by the knott's test enables dirofilaria immitis and d. repens species-specific and acanthocheilonema (syn. dipetalonema) genus-specific diagnosis.considering the increasing importance of small animals travel medicine and the spread of filariae with zoonotic potential to non-endemic european areas, routine filarial diagnosis in dogs is becoming important. dirofilaria immitis, d. repens, acanthocheilonema dracunculoides and a. reconditum are the most common canine filarial nematodes presenting blood circulating microfilariae (mf) which can be differentiated to species level by the acid phosphatase activity patterns or by pcr. available data ...201323442771
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