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evidence for multiplication of the leafhopper-borne maize yellow stripe virus in its vector using elisa and dot-blot hybridization.maize yellow stripe virus (mysv) has several features in common with tenuiviruses, but is transmitted by a leafhopper, cicadulina chinai (cicadellidae, hemiptera), rather than planthoppers (delphacidae, hemiptera). herein, mysv was shown to be propagatively transmitted like tenuiviruses. mysv rna was not detected in leafhoppers by dot blot hybridization one day following a 2-day acquisition access period (aap), but was detected in single or groups of leafhoppers 5-20 days post-acquisition. likew ...200717136504
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