| development of pcr primer systems for amplification of nitrite reductase genes (nirk and nirs) to detect denitrifying bacteria in environmental samples. | a system was developed for the detection of denitrifying bacteria by the amplification of specific nitrite reductase gene fragments with pcr. primer sequences were found for the amplification of fragments from both nitrite reductase genes (nirk and nirs) after comparative sequence analysis. whenever amplification was tried with these primers, the known nir type of denitrifying laboratory cultures could be confirmed. likewise, the method allowed a determination of the nir type of five laboratory ... | 1998 | 9758798 |
| rapid ribosequencing--an effective diagnostic tool for detecting microbial infection. | rapid and reliable identification of microorganisms is a prerequisite for the diagnosis and subsequent treatment of infectious diseases. the identification of pathogenic bacteria is traditionally based on their isolation from clinical samples and propagation on culture medium in the routine laboratory. however, despite clinical signs of infection, culture of the pathogenic agent often fails. this may be due to a low number of microorganisms, prior antibiotic treatment, nonculturable microorganis ... | 2001 | 11261751 |
| the aquatic budding bacterium blastobacter denitrificans is a nitrogen-fixing symbiont of aeschynomene indica. | blastobacter spp. are freshwater bacteria that form rosette structures by cellular attachment to a common base. comparative analyses of ribosomal 16s rrna gene and internally transcribed spacer region sequences indicated that b. denitrificans is a member of the alpha-subdivision of proteobacteria. among the alpha-proteobacteria, b. denitrificans was related to a cluster of genera, including rhodopseudomonas palustris, afipia felis, nitrobacter hamburgensis, and bradyrhizobium spp. although the p ... | 2002 | 11872460 |
| disseminated infection due to blastobacter denitrificans following routine appendectomy in an adolescent. | until now, blastobacter denitrificans has not been mentioned in the context of human infections. a case of severe complication caused by b. denitrificans after routine appendectomy in a young girl is described and confirms this organism to be an opportunistic human pathogen. | 2002 | 12202615 |
| molecular diversity of denitrifying genes in continental margin sediments within the oxygen-deficient zone off the pacific coast of mexico. | to understand the composition and structure of denitrifying communities in the oxygen-deficient zone off the pacific coast of mexico, the molecular diversity of nir genes from sediments obtained at four stations was examined by using a pcr-based cloning approach. a total of 50 operational taxonomic units (otus) for nirk and 82 otus for nirs were obtained from all samples. forty-four of the nirs clones and 31 of the nirk clones were sequenced; the levels of similarity of the nirs clones were 52 t ... | 2003 | 12788762 |
| phylogenetic analysis of bradyrhizobium japonicum and photosynthetic stem-nodulating bacteria from aeschynomene species grown in separated geographical regions. | nearly complete and short partial 16s rrna sequences were derived from pcr-amplified ribosomal dnas of bradyrhizobium japonicum usda 136 and usda 110 and five strains of bacteriochlorophyll-synthesizing bacteria isolated from stem nodules of aeschynomene indica and other aeschynomene species growing in different geographic regions, including india, the philippines and north america. we confirmed that the five stem-nodulating strains examined synthesize bacteriochlorophyll a, and the absorption s ... | 1994 | 16349221 |
| genetic and phenetic analyses of bradyrhizobium strains nodulating peanut (arachis hypogaea l.) roots. | seventeen bradyrhizobium sp. strains and one azorhizobium strain were compared on the basis of five genetic and phenetic features: (i) partial sequence analyses of the 16s rrna gene (rdna), (ii) randomly amplified dna polymorphisms (rapd) using three oligonucleotide primers, (iii) total cellular protein profiles, (iv) utilization of 21 aliphatic and 22 aromatic substrates, and (v) intrinsic resistances to seven antibiotics. partial 16s rdna analysis revealed the presence of only two rdna homolog ... | 1995 | 7538280 |
| phenotypic and genotypic characterization of bradyrhizobia nodulating the leguminous tree acacia albida. | rhizobial isolates that were obtained from both surface and deep soil samples in the sahelian and sudano-guinean areas of senegal (west africa) under acacia albida trees were compared with representative strains of known rhizobial species and genera. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of proteins was used to determine the taxonomic positions of these organisms and the relationships between isolates obtained from the surface and isolates obtained from deep soil. ... | 1994 | 7520737 |
| evidence for a close genealogical relationship between afipia (the causal organism of cat scratch disease), bradyrhizobium japonicum and blastobacter denitrificans. | the primary structures of the small subunit rrna of bradyrhizobium japonicum and blastobacter denitrificans were determined by direct sequencing of enzymatically amplified dna. comparative sequence analysis revealed that bradyrhizobium japonicum and blastobacter denitrificans are members of the alpha-2 subgroup of the proteobacteria and show a very close phylogenetic relationship with the genus afipia. | 1992 | 1383084 |
| reclassification of agromonas oligotrophica into the genus bradyrhizobium as bradyrhizobium oligotrophicum comb. nov. | agromonas oligotrophica jcm 1494(t) was isolated in japan in 1983, and the name was validly published in 1985. analysis of the 16s rrna gene sequence showed that agromonas oligotrophica lmg 10732(t) ( = jcm 1494(t)) is located within the genus bradyrhizobium, with bradyrhizobium denitrificans lmg 8443(t) as its closest relative, showing 99.6 % 16s rrna gene sequence identity. however, agromonas oligotrophica lmg 10732(t) and bradyrhizobium denitrificans lmg 8443(t) can be distinguished by housek ... | 2013 | 22685107 |
| microbially influenced corrosion of galvanized steel pipes in aerobic water systems. | to investigate the role of heterotrophic bacteria in the corrosion of galvanized steel in the presence of water. | 2010 | 20070443 |
| proposal for combining bradyrhizobium spp. (aeschynomene indica) with blastobacter denitrificans and to transfer blastobacter denitrificans (hirsch and muller, 1985) to the genus bradyrhizobium as bradyrhizobium denitrificans (comb. nov.). | the symbiotic bradyrhizobia of aeschynomene indica and the aquatic budding bacterium blastobacter denitrificans have much in common and this study broadens the characters that are shared between the two. the 23s rrna gene sequences of the bradyrhizobial isolates were most similar to each other and to the sequence of bl. denitrificans. evidence for the presence of photosynthetic genes in the genome of bl. denitrificans was obtained by pcr using primers to the conserved m subunit (pufm) of the pho ... | 2006 | 16564957 |
| bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digester. | a strictly aerobic, mesophilic bacterium, strain amx 51(t), was isolated from anaerobic digester sludge. cells were gram-negative, motile, non-sporulating, straight to curved rods with one polar flagellum. the isolate had phenotypic traits of the genus bosea, including cellular fatty acid and substrate utilization profiles. physiological characteristics and antibiotic susceptibility were determined. phylogenetic analysis revealed that strain amx 51(t) was a member of the alpha-proteobacteria, mo ... | 2003 | 13130002 |
| changing concepts in the systematics of bacterial nitrogen-fixing legume symbionts. | as of february 2003, bacteria that form nitrogen-fixing symbiotic associations with legumes have been confirmed in 44 species of 12 genera. phylogenies of these taxa containing legume symbionts based on the comparative analysis of 16s rdna sequences show that they are not clustered in one lineage but are distributed in the classes alphaproteobacteria and betaproteobacteria, and dispersed over the following nine monophyletic groups, being intermingled with other taxa that do not contain legume sy ... | 2003 | 12949698 |
| phenotypic and genotypic diversity of rhizobia nodulating pterocarpus erinaceus and p. lucens in senegal. | a total of fifty root nodules isolates of fast-growing and slow growing rhizobia from pterocarpus ennaceus and pterocarpus lucens respectively native of sudanean and sahelian regions of senegal were characterized. these isolates were compared to representative strains of known rhizobial species. twenty-two new isolates were slow growers and twenty-eight were fast growers. a polyphasic approach was performed including comparative total protein sodium dodecyl sulphate polyacrylamide gel (sds-page) ... | 2002 | 12583718 |