| chickpea chlorotic stunt virus: a new polerovirus infecting cool-season food legumes in ethiopia. | abstract serological analysis of diseased chickpea and faba bean plantings with yellowing and stunting symptoms suggested the occurrence of an unknown or uncommon member of the family luteoviridae in ethiopia. degenerate primers were used for reverse transcriptase-polymerase chain reaction amplification of the viral coat protein (cp) coding region from both chickpea and faba bean samples. cloning and sequencing of the amplicons yielded nearly identical (96%) nucleotide sequences of a previously ... | 2006 | 18944302 |
| epidemiology and integrated management of persistently transmitted aphid-borne viruses of legume and cereal crops in west asia and north africa. | cool-season food legumes (faba bean, lentil, chickpea and pea) and cereals (bread and durum wheat and barley) are the most important and widely cultivated crops in west asia and north africa (wana), where they are the main source of carbohydrates and protein for the majority of the population. persistently transmitted aphid-borne viruses pose a significant limitation to legume and cereal production worldwide. surveys conducted in many countries in wana during the last three decades established t ... | 2009 | 19152820 |
| molecular, serological and biological variation among chickpea chlorotic stunt virus isolates from five countries of north africa and west asia. | chickpea chlorotic stunt virus (cpcsv), a proposed new member of the genus polerovirus (family luteoviridae), has been reported only from ethiopia. in attempts to determine the geographical distribution and variability of cpcsv, a pair of degenerate primers derived from conserved domains of the luteovirus coat protein (cp) gene was used for rt-pcr analysis of various legume samples originating from five countries and containing unidentified luteoviruses. sequencing of the amplicons provided evid ... | 2009 | 19347243 |
| complete genome sequences of cowpea polerovirus 1 and cowpea polerovirus 2 infecting cowpea plants in burkina faso. | the full-length genome sequences of two novel poleroviruses found infecting cowpea plants, cowpea polerovirus 1 (cppv1) and cowpea polerovirus 2 (cppv2), were determined using overlapping rt-pcr and race-pcr. whereas the 5845-nt cppv1 genome was most similar to chickpea chlorotic stunt virus (73% identity), the 5945-nt cppv2 genome was most similar to phasey bean mild yellow virus (86% identity). the cppv1 and cppv2 genomes both have a typical polerovirus genome organization. phylogenetic analys ... | 2017 | 28337544 |
| nucleotide sequence of a chickpea chlorotic stunt virus relative that infects pea and faba bean in china. | we determined the genome sequence of a new polerovirus that infects field pea and faba bean in china. its entire nucleotide sequence (6021 nt) was most closely related (83.3% identity) to that of an ethiopian isolate of chickpea chlorotic stunt virus (cpcsv-eth). with the exception of the coat protein (encoded by orf3), amino acid sequence identities of all gene products of this virus to those of cpcsv-eth and other poleroviruses were <90%. this suggests that it is a new member of the genus pole ... | 2012 | 22476900 |