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pseudozyma hubeiensis sp. nov. and pseudozyma shanxiensis sp. nov., novel ustilaginomycetous anamorphic yeast species from plant leaves.among basidiomycetous yeast strains isolated from wilting leaves of various plants in china, two groups of pseudozyma strains were distinguished from the others by morphological and physiological characterization. molecular taxonomic analysis based on sequencing of the large subunit (26s) rrna gene d1/d2 domain and internal transcribed spacer (its) region confirmed that the two groups represent two novel species. they are proposed as pseudozyma hubeiensis sp. nov. (type strain ws 6.4t = as 2.249 ...200616403900
production of different types of mannosylerythritol lipids as biosurfactants by the newly isolated yeast strains belonging to the genus pseudozyma.mannosylerythritol lipids (mel), which are abundantly secreted by yeasts, are one of the most promising biosurfactants known. to obtain various types of mel and to attain a broad range of applications for them, screening of novel producers was undertaken. thirteen strains of yeasts were successfully isolated as potential mel producers; they showed high production yields of mel of around 20 g l(-1) from 40 g l(-1) of soybean oil. based on the taxonomical study, all the strains were classified to ...200717505770
efficient production of mannosylerythritol lipids with high hydrophilicity by pseudozyma hubeiensis km-59.mannosylerythritol lipids (mels) are one of the most promising biosurfactants known because of their multifunctionality and biocompatibility. a previously isolated yeast strain, pseudozyma sp. km-59, mainly produced a hydrophilic mel, namely mel-c (4-o-[4'-o-acetyl-2',3'-di-o-alka(e)noyl-beta-d: -mannopyranosyl]-d: -erythritol). in this study, we taxonomically characterize the strain in detail and investigate the culture conditions. the genetic, morphological, and physiological characteristics o ...200818071643
biochemical characterization of two xylanases from yeast pseudozyma hubeiensis producing only xylooligosaccharides.two distinct xylanases from pseudozyma hubeiensis ncim 3574 were purified to homogeneity. the molecular masses of two native xylanases were 33.3 kda (phx33) and 20.1 kda (phx20). phx33 is predominant with alpha-helix and phx20 contained predominantly beta-sheets. xylanase, phx33, possesses three tryptophan and one carboxyl residues at the active site. the active site of phx20 comprises one residue each of tryptophan, carboxyl and histidine. carboxyl residue is mainly involved in catalysis and tr ...200919692229
isolation of a lipase-secreting yeast for enzyme production in a pilot-plant scale batch fermentation.the production of lipase by twenty-nine yeasts isolated from the phylloplane of hibiscus rosa-sinensis was evaluated. the highest lipase producers were pseudozyma hubeiensis hb85a, debaryomyces occidentalis-like hb83 and cryptococcus sp. hb80. p. hubeiensis hb85a batch fermentations were carried out in a bioreactor and lipase production improved 3.2-fold as compared to flask submerged cultures. the production process was significantly reduced from 48 h (in flasks) to 18 h (in the bioreactor). th ...201019700311
biosurfactant-producing yeast isolated from calyptogena soyoae (deep-sea cold-seep clam) in the deep sea.we describe a detailed structure determination of biosurfactant produced by pseudozyma hubeiensis sy62, which was newly isolated from calyptogena soyoae (deep-sea cold-seep clam, shirouri-gai) at 1156 m in sagami bay. p. hubeiensis sy62 was taxonomically slightly different from the p. hubeiensis type strain, which produces biosurfactants. glycolipid production by the strain was also slightly different from those of previously reported strains. bs productivity was estimated to be around 30 g/l fr ...201020547357
yeast extract stimulates production of glycolipid biosurfactants, mannosylerythritol lipids, by pseudozyma hubeiensis sy62.we improved the culture conditions for a biosurfactant producing yeast, pseudozyma hubeiensis sy62. we found that yeast extract greatly stimulates mel production. furthermore, we demonstrated a highly efficient production of mels in the improved medium by fed-batch cultivation. the final concentration of mels reached 129 -¦ 8.2g/l for one week.201121393057
the assessment of epiphytic yeast diversity in sugarcane phyllosphere in thailand by culture-independent method.the diversity of epiphytic yeasts from sugarcane (saccharum officinarum linn.) phyllospheres in thailand was investigated by culture-independent method based on the analysis of the d1/d2 domains of the large subunit rrna gene sequences. forty-five samples of sugarcane leaf were collected randomly from ten provinces in thailand. a total of 1342 clones were obtained from 45 clone libraries. 426 clones (31.7 %) were closely related to yeast strains in the genbank database, and they were clustered i ...201526615738
yeast diversity and novel yeast d1/d2 sequences from corn phylloplane obtained by a culture-independent approach.culture-independent techniques have recently been used for evaluation of microbial diversity in the environment since it addresses the problem of unculturable microorganisms. in this study, the diversity of epiphytic yeasts from corn (zea mays linn.) phylloplanes in thailand was investigated using this technique and sequence-based analysis of the d1/d2 domains of the large subunit ribosomal dna sequences. thirty-seven samples of corn leaf were collected randomly from 10 provinces. the dna was ex ...201627578202
purification and characterization of an extracellular β-xylosidase from pseudozyma hubeiensis ncim 3574 (phxyl), an unexplored yeast.this paper reports on the production of β-xylosidase from an unexplored yeast, pseudozyma hubeinsis. the expression of this enzyme could be induced by beech wood xylan when the yeast was grown at 27 °c. the enzyme was purified to homogeneity as a glycoprotein with 23 % glycosylation. the purification protocol involved ammonium sulphate precipitation, qae-sephadex a50 ion exchange chromatography and sephacryl-200 column chromatography which resulted in 8.3-fold purification with 53.12 % final rec ...201627637943
lipid production through simultaneous utilization of glucose, xylose, and l-arabinose by pseudozyma hubeiensis: a comparative screening study.co-fermentation of glucose, xylose and l-arabinose from lignocellulosic biomass by an oleaginous yeast is anticipated as a method for biodiesel production. however, most yeasts ferment glucose first before consuming pentoses, due to glucose repression. this preferential utilization results in delayed fermentation time and lower productivity. therefore, co-fermentation of lignocellulosic sugars could achieve cost-effective conversion of lignocellulosic biomass to microbial lipid. comprehensive sc ...201627566647
efficient and simple electro-transformation of intact cells for the basidiomycetous fungus pseudozyma hubeiensis.an electroporation procedure for the species was investigated to develop an efficient transformation method for the basidiomycetous fungus pseudozyma hubeiensis sy62, a strong biosurfactant-producing host.201525851954
draft genome sequence of the basidiomycetous yeast-like fungus pseudozyma hubeiensis sy62, which produces an abundant amount of the biosurfactant mannosylerythritol lipids.the basidiomycetous yeast-like fungus pseudozyma hubeiensis strain sy62 is capable of producing an abundant amount of the glycolipid biosurfactant mannosylerythritol lipids (mels), which are a major component of monoacetylated mel (mel-c). to reveal the synthetic pathway of the mels of strain sy62, we present the 18.44-mb draft genome sequence.201323814110
accumulation of cellobiose lipids under nitrogen-limiting conditions by two ustilaginomycetous yeasts, pseudozyma aphidis and pseudozyma hubeiensis.some basidiomycetous yeast strains extracellularly produce cellobiose lipids (cls), glycolipid biosurfactants which have strong fungicidal activity. the representative cl producer ustilago maydis produces cls together with the other glycolipids, mannosylerythritol lipids (mels); the preference of the two glycolipids is affected considerably by the nitrogen source. to develop new cl producers, 12 mel producers were cultured under the nitrogen-limited conditions. pseudozyma aphidis and pseudozyma. ...201322985214
glycolipid biosurfactants, mannosylerythritol lipids, show antioxidant and protective effects against h(2)o(2)-induced oxidative stress in cultured human skin fibroblasts.mannosylerythritol lipids (mels) are biosurfactants known for their versatile interfacial and biochemical properties. to broaden their application in cosmetics, we investigated the antioxidant properties of different mel derivatives (mel-a, -b, and -c) by using a 1,1-diphenyl-2-picryl hydrazine (dpph) free-radical- and superoxide anion-scavenging assay. all mel derivatives tested showed antioxidant activity in vitro, but at lower levels than those of arbutin. of the mels, mel-c, which is produce ...201222864517
optimal conditions for continuous immobilization of pseudozyma hubeiensis (strain hb85a) lipase by adsorption in a packed-bed reactor by response surface methodology.this study aimed to develop an optimal continuous process for lipase immobilization in a bed reactor in order to investigate the possibility of large-scale production. an extracellular lipase of pseudozyma hubeiensis (strain hb85a) was immobilized by adsorption onto a polystyrene-divinylbenzene support. furthermore, response surface methodology (rsm) was employed to optimize enzyme immobilization and evaluate the optimum temperature and ph for free and immobilized enzyme. the optimal immobilizat ...201222315670
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