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structural and functional characterization of gene clusters directing nonribosomal synthesis of bioactive cyclic lipopeptides in bacillus amyloliquefaciens strain fzb42.the environmental strain bacillus amyloliquefaciens fzb42 promotes plant growth and suppresses plant pathogenic organisms present in the rhizosphere. we sampled sequenced the genome of fzb42 and identified 2,947 genes with >50% identity on the amino acid level to the corresponding genes of bacillus subtilis 168. six large gene clusters encoding nonribosomal peptide synthetases (nrps) and polyketide synthases (pks) occupied 7.5% of the whole genome. two of the pks and one of the nrps encoding gen ...200414762003
tryptophan-dependent production of indole-3-acetic acid (iaa) affects level of plant growth promotion by bacillus amyloliquefaciens fzb42.phytohormone-like acting compounds previously have been suggested to be involved in the phytostimulatory action exerted by the plant-beneficial rhizobacterium bacillus amyloliquefaciens fzb42. analyses by high-performance liquid chromatography and gas chromatography-mass spectrometry performed with culture filtrates of fzb42 demonstrated the presence of indole-3-acetic acid (iaa), corroborating it as one of the pivotal plant-growth-promoting substances produced by this bacterium. in the presence ...200717555270
comparative analysis of the complete genome sequence of the plant growth-promoting bacterium bacillus amyloliquefaciens fzb42.bacillus amyloliquefaciens fzb42 is a gram-positive, plant-associated bacterium, which stimulates plant growth and produces secondary metabolites that suppress soil-borne plant pathogens. its 3,918-kb genome, containing an estimated 3,693 protein-coding sequences, lacks extended phage insertions, which occur ubiquitously in the closely related bacillus subtilis 168 genome. the b. amyloliquefaciens fzb42 genome reveals an unexpected potential to produce secondary metabolites, including the polyke ...200717704766
degu and ycze positively regulate the synthesis of bacillomycin d by bacillus amyloliquefaciens strain fzb42.environmental strain bacillus amyloliquefaciens fzb42 differs from the domesticated model organism of the same genus, bacillus subtilis 168, in its ability to promote plant growth and suppress plant-pathogenic organisms present in the rhizosphere. this behavior is exerted mainly through the production of several nonribosomal cyclic lipopeptides and polyketides, which exhibit a broad range of action against phytopathogenic bacteria, fungi, and nematodes. here, we provide evidence that the synthes ...200717827323
macrolactin is the polyketide biosynthesis product of the pks2 cluster of bacillus amyloliquefaciens fzb42.in the genome of bacillus amyloliquefaciens fzb42, three operons pks1, pks2, and pks3 were identified which encode the biosynthesis of polyketides. pks1 and pks3 have been attributed to the production of bacillaene and difficidin/oxydifficidin, respectively, while the pks2 product remained hitherto unknown. mass spectrometric analysis of the culture filtrates of the wild-type b. amyloliquefaciens fzb42 and mutants revealed pks2-specific metabolites. by combination of the mass spectrometric and u ...200717844999
more than anticipated - production of antibiotics and other secondary metabolites by bacillus amyloliquefaciens fzb42.the genome of environmental bacillus amyloliquefaciens fzb42 harbors numerous gene clusters involved in synthesis of antifungal and antibacterial acting secondary metabolites. five gene clusters, srf, bmy, fen, nrs, dhb, covering altogether 137 kb, direct non-ribosomal synthesis of the cyclic lipopeptides surfactin, bacillomycin, fengycin, an unknown peptide, and the iron siderophore bacillibactin. bacillomycin and fengycin were shown to act against phytopathogenic fungi in a synergistic manner. ...200918957859
genome analysis of bacillus amyloliquefaciens fzb42 reveals its potential for biocontrol of plant pathogens.the genome of plant-associated bacillus amyloliquefaciens fzb42 harbors an array of giant gene clusters involved in synthesis of lipopeptides and polyketides with antifungal, antibacterial and nematocidal activity. five gene clusters, srf, bmy, fen, nrs, dhb, covering altogether 137 kb, were shown to direct synthesis of the cyclic lipopeptides surfactin, bacillomycin, fengycin, an unknown peptide, and the iron-siderophore bacillibactin. in addition, one gene cluster encoding enzymes involved in ...200919041913
crystallization and preliminary x-ray analysis of the lov domain of the blue-light receptor ytva from bacillus amyloliquefaciens fzb42.light-oxygen-voltage (lov) proteins play an important role in blue-light-dependent physiological processes in many organisms. the lov domain of the blue-light receptor ytva from bacillus amyloliquefaciens fzb42 has been purified and crystallized at 277 k using the sitting-drop vapour-diffusion method with 2-ethoxyethanol as a precipitant. a data set was collected to 1.60 a resolution from a single crystal at 100 k using synchrotron radiation. the lov domain of ytva crystallized in space group c2 ...200919652358
the final steps of bacillaene biosynthesis in bacillus amyloliquefaciens fzb42: direct evidence for beta,gamma dehydration by a trans-acyltransferase polyketide synthase. 201020087918
relationship of bacillus amyloliquefaciens clades associated with strains dsm7t and fzb42: a proposal for bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on their discriminating complete genome sequences.the whole genome sequenced rhizobacterium fzb42 (chen et al., 2007) and other plant-associated bacillus strains either designated as bacillus amyloliquefaciens or bacillus subtilis are used commercially to promote growth and health of crop plants. previous investigations revealed that the strains represent an own ecotype related to b. amyloliquefaciens, however its exact taxonomic position remains elusive (reva et al., 2004). here we have demonstrated ability to colonize arabidopsis roots for a ...201020817842
plantazolicin, a novel microcin b17/streptolysin s-like natural product from bacillus amyloliquefaciens fzb42.here we report on a novel thiazole/oxazole-modified microcin (tomm) from bacillus amyloliquefaciens fzb42, a gram-positive soil bacterium. this organism is well known for stimulating plant growth and biosynthesizing complex small molecules that suppress the growth of bacterial and fungal plant pathogens. like microcin b17 and streptolysin s, the tomm from b. amyloliquefaciens fzb42 undergoes extensive posttranslational modification to become a bioactive natural product. our data show that the mo ...201020971906
efficient colonization of plant roots by the plant growth promoting bacterium bacillus amyloliquefaciens fzb42, engineered to express green fluorescent protein.a single copy of the gfp gene linked with the p(spac) promoter and flanked by the terminal fzb42 amye sequences was stably integrated into the chromosome of plant growth promoting bacterium bacillus amyloliquefaciens fzb42 via homologous recombination. a spontaneous mutant, fb01mut, emitting bright fluorescence was detected among the transformants and found suitable for colonization experiments performed with zea mays, arabidopsis thaliana and lemna minor. real-time rt-pcr revealed that fb01mut ...201121237217
characterization of bacillus subtilis hc8, a novel plant-beneficial endophytic strain from giant hogweed.thirty endophytic bacteria were isolated from various plant species growing near saint-petersburg, russia. based on a screening for various traits, including plant-beneficial properties and dna fragment patterns, potential siblings were removed. the remaining isolates were taxonomically identified using 16s rdna sequences and potential human and plant pathogens were removed. the remaining strains were tested for their ability to promote radish root growth and to protect tomato plants against tom ...201121366893
plantazolicin a and b: structure elucidation of ribosomally synthesized thiazole/oxazole peptides from bacillus amyloliquefaciens fzb42.the structures of the ribosomally synthesized peptide antibiotics from bacillus amyloliquefaciens fzb42, plantazolicin a and b, have been elucidated by high resolving esi-msms, 2d (1)h-(13)c-correlated nmr spectroscopy as well as (1)h-(15)n-hmqc/(1)h-(15)n-hmbc nmr experiments. (15)n-labeling prior to the experiments facilitated the structure determination, unveiling a hitherto unusual number of thiazoles and oxazoles formed from a linear 14mer precursor peptide. this finding further extends the ...201121568297
expression of the lantibiotic mersacidin in bacillus amyloliquefaciens fzb42.lantibiotics are small peptide antibiotics that contain the characteristic thioether amino acids lanthionine and methyllanthionine. as ribosomally synthesized peptides, lantibiotics possess biosynthetic gene clusters which contain the structural gene (lana) as well as the other genes which are involved in lantibiotic modification (lanm, lanb, lanc, lanp), regulation (lanr, lank), export (lant(p)) and immunity (lanefg). the lantibiotic mersacidin is produced by bacillus sp. hil y-85,54728, which ...201121811596
Structure determination and interception of biosynthetic intermediates for the plantazolicin class of highly discriminating antibiotics.The soil-dwelling, plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42 is a prolific producer of complex natural products. Recently, a new FZB42 metabolite, plantazolicin (PZN), has been described as a member of the growing thiazole/oxazole-modified microcin (TOMM) family. TOMMs are biosynthesized from inactive, ribosomal peptides and undergo a series of cyclodehydrations, dehydrogenations, and other modifications to become bioactive natural products. Using high-resolution mass spe ...201121950656
linking plant nutritional status to plant-microbe interactions.plants have developed a wide-range of adaptations to overcome nutrient limitation, including changes to the quantity and composition of carbon-containing compounds released by roots. root-associated bacteria are largely influenced by these compounds which can be perceived as signals or substrates. here, we evaluate the effect of root exudates collected from maize plants grown under nitrogen (n), phosphate (p), iron (fe) and potassium (k) deficiencies on the transcriptome of the plant growth prom ...201323874669
developmentally-regulated excision of the spβ prophage reconstitutes a gene required for spore envelope maturation in bacillus subtilis.temperate phages infect bacteria by injecting their dna into bacterial cells, where it becomes incorporated into the host genome as a prophage. in the genome of bacillus subtilis 168, an active prophage, spβ, is inserted into a polysaccharide synthesis gene, spsm. here, we show that a rearrangement occurs during sporulation to reconstitute a functional composite spsm gene by precise excision of spβ from the chromosome. spβ excision requires a putative site-specific recombinase, spra, and an acce ...201425299644
biocontrol mechanism by root-associated bacillus amyloliquefaciens fzb42 - a review.bacillus amyloliquefaciens subsp. plantarum fzb42 is a gram-positive model bacterium for unraveling plant-microbe interactions in bacilli. in addition, fzb42 is used commercially as biofertilizer and biocontrol agent in agriculture. genome analysis of fzb42 revealed that nearly 10% of the fzb42 genome is devoted to synthesizing antimicrobial metabolites and their corresponding immunity genes. however, recent investigations in planta demonstrated that - except surfactin - the amount of such compo ...201526284057
bacillus volatiles adversely affect the physiology and ultra-structure of ralstonia solanacearum and induce systemic resistance in tobacco against bacterial wilt.volatile organic compounds (vocs) produced by various bacteria have significant potential to enhance plant growth and to control phytopathogens. six of the most effective antagonistic bacillus spp. were used in this study against ralstonia solanacearum (rsc) tbbs1, the causal agent of bacterial wilt disease in tobacco. bacillus amyloliquefaciens fzb42 and bacillus artrophaeus lssc22 had the strongest inhibitory effect against rsc. thirteen vocs produced by fzb42 and 10 by lssc22 were identified ...201728091587
transcriptomic profiling of bacillus amyloliquefaciens fzb42 in response to maize root exudates.plant root exudates have been shown to play an important role in mediating interactions between plant growth-promoting rhizobacteria (pgpr) and their host plants. most investigations were performed on gram-negative rhizobacteria, while much less is known about gram-positive rhizobacteria. to elucidate early responses of pgpr to root exudates, we investigated changes in the transcriptome of a gram-positive pgpr to plant root exudates.201222720735
collagen-like proteins (clpa, clpb, clpc, and clpd) are required for biofilm formation and adhesion to plant roots by bacillus amyloliquefaciens fzb42.the genes of collagen-like proteins (clps) have been identified in a broad range of bacteria, including some human pathogens. they are important for biofilm formation and bacterial adhesion to host cells in some human pathogenic bacteria, including several bacillus spp. strains. interestingly, some bacterial clp-encoding genes (clps) have also been found in non-human pathogenic strains such as b. cereus and b. amyloliquefaciens, which are types of plant-growth promoting rhizobacteria (pgpr). in ...201525658640
the impact of the pathogen rhizoctonia solani and its beneficial counterpart bacillus amyloliquefaciens on the indigenous lettuce microbiome.lettuce belongs to the most commonly raw eaten food worldwide and its microbiome plays an important role for both human and plant health. yet, little is known about the impact of potentially occurring pathogens and beneficial inoculants of the indigenous microorganisms associated with lettuce. to address this question we studied the impact of the phytopathogenic fungus rhizoctonia solani and the biological control agent bacillus amyloliquefaciens fzb42 on the indigenous rhizosphere and phyllosph ...201424795707
drna-seq reveals genomewide tsss and noncoding rnas of plant beneficial rhizobacterium bacillus amyloliquefaciens fzb42.bacillus amyloliquefaciens subsp. plantarum fzb42 is a representative of gram-positive plant-growth-promoting rhizobacteria (pgpr) that inhabit plant root environments. in order to better understand the molecular mechanisms of bacteria-plant symbiosis, we have systematically analyzed the primary transcriptome of strain fzb42 grown under rhizosphere-mimicking conditions using differential rna sequencing (drna-seq). our analysis revealed 4,877 transcription start sites for protein-coding genes, id ...201526540162
difficidin and bacilysin from bacillus amyloliquefaciens fzb42 have antibacterial activity against xanthomonas oryzae rice pathogens.bacterial blight and bacterial leaf streak are serious, economically damaging, diseases of rice caused by the bacteria xanthomonas oryzae pv. oryzae and x. oryzae pv. oryzicola. bacillus amyloliquefaciens fzb42 was shown to possess biocontrol activity against these xanthomonas strains by producing the antibiotic compounds difficidin and bacilysin. analyses using fluorescence, scanning electron and transmission electron microscopy revealed difficidin and bacilysin caused changes in the cell wall ...201526268540
glycin-rich antimicrobial peptide yd1 from b. amyloliquefaciens, induced morphological alteration in and showed affinity for plasmid dna of e. coli.antimicrobial peptides (amps), low-molecular-weight proteins with broad-spectrum antimicrobial activity, are the most promising candidates for the development of novel antimicrobials. a powerful cationic glycine-rich amp yd1 (mw ~ 1.0 kda) was purified from bacillus amyloliquefaciens cbsyd1 isolated from traditional korean fermented food kimchi, for the treatment of multidrug-resistant (mdr) bacteria. strain cbsyd1 was identified 99.79% similar to bacillus amyloliquefaciens subsp. plantarum fzb4 ...201728050849
bacilysin from bacillus amyloliquefaciens fzb42 has specific bactericidal activity against harmful algal bloom species.harmful algal blooms, caused by massive and exceptional overgrowth of microalgae and cyanobacteria, are a serious environmental problem worldwide : in the present study, we looked for bacillus strains with sufficiently strong anticyanobacterial activity to be used as biocontrol agents. among 24 strains, bacillus amyloliquefaciens fzb42 showed the strongest bactericidal activity against microcystis aeruginosa, with a kill rate of 98.78%. the synthesis of the anticyanobacterial substance did not d ...201425261512
amylocyclicin, a novel circular bacteriocin produced by bacillus amyloliquefaciens fzb42.bacillus amyloliquefaciens fzb42 is a gram-positive plant growth-promoting bacterium with an impressive capacity to synthesize nonribosomal secondary metabolites with antimicrobial activity. here we report on a novel circular bacteriocin which is ribosomally synthesized by fzb42. the compound displayed high antibacterial activity against closely related gram-positive bacteria. transposon mutagenesis and subsequent site-specific mutagenesis combined with matrix-assisted laser desorption ionizatio ...201424610713
plantazolicin is an ultra-narrow spectrum antibiotic that targets the bacillus anthracis membrane.plantazolicin (pzn) is a ribosomally synthesized and post-translationally modified natural product from bacillus methylotrophicus fzb42 and bacillus pumilus. extensive tailoring to twelve of the fourteen amino acid residues in the mature natural product endows pzn with not only a rigid, polyheterocyclic structure, but also antibacterial activity. here we report a remarkably discriminatory activity of pzn toward bacillus anthracis, which rivals a previously-described gamma (γ) phage lysis assay i ...201627152321
a phosphate starvation-inducible ribonuclease of bacillus licheniformis.the bli03719 protein of bacillus licheniformis dsm13 belongs to the most abundant extracellular proteins under phosphate starvation conditions. in this study, the function of this phosphate starvation inducible protein was determined. an amino-acid sequence analysis of the bli03719-encoding gene showed a high similarity with genes encoding the barnase of bacillus amyloliquefaciens fzb42 and binase-like rnase of bacillus pumilus sarf-032. the comparison of the control strain and a bli03719-defici ...201627160576
influence of promoters on the production of fengycin in bacillus spp.fengycin is a promising antifungal lipopeptide from bacillus spp. synthesized by non-ribosomal peptide synthetases (nrps). in this work, fengycin production of a spontaneous fengycin overproducing strain, bacillus subtilis bbg21, was first compared to those of b. subtilis bbg111 (a 168 derivative), b. subtilis atcc 21332 and bacillus amyloliquefaciens fzb42 under two different experimental conditions. in both conditions, very high fengycin yields were obtained from strain bbg21 (480 mg/l) in com ...201626912322
cyclic lipopeptides of bacillus amyloliquefaciens subsp. plantarum colonizing the lettuce rhizosphere enhance plant defense responses toward the bottom rot pathogen rhizoctonia solani.the commercially available inoculant bacillus amyloliquefaciens fzb42 is able to considerably reduce lettuce bottom rot caused by rhizoctonia solani. to understand the interaction between fzb42 and r. solani in the rhizosphere of lettuce, we used an axenic system with lettuce bacterized with fzb42 and inoculated with r. solani. confocal laser scanning microscopy showed that fzb42 could delay the initial establishment of r. solani on the plants. to show which secondary metabolites of fzb42 are pr ...201526011557
malonylome of the plant growth promoting rhizobacterium with potent biocontrol activity, bacillus amyloliquefaciens fzb42.the data presented in this article are related to the publication entitled "malonylome analysis of rhizobacterium bacillus amyloliquefaciens fzb42 reveals involvement of lysine malonylation in polyketide synthesis and plant-bacteria interactions"(doi:10.1016/j.jprot.2016.11.022) (b. fan, y. li, l. li et al.) [1]. this article presented the raw information of all malonyllysine sites identified by lc-ms/ms in the bacillus amyloliquefaciens fzb42. further, the functional features and conservation o ...201728070544
malonylome analysis of rhizobacterium bacillus amyloliquefaciens fzb42 reveals involvement of lysine malonylation in polyketide synthesis and plant-bacteria interactions.using the combination of affinity enrichment and high-resolution lc-ms/ms analysis, we performed a large-scale lysine malonylation analysis in the model representative of gram-positive plant growth-promoting rhizobacteria (pgpr), bacillus amyloliquefaciens fzb42. altogether, 809 malonyllysine sites in 382 proteins were identified. the bioinformatic analysis revealed that lysine malonylation occurs on the proteins involved in a variety of biological functions including central carbon metabolism, ...201727939684
new sigd-regulated genes identified in the rhizobacterium bacillus amyloliquefaciens fzb42.the alternative sigma factor d is known to be involved in at least three biological processes in bacilli: flagellin synthesis, methyl-accepting chemotaxis and autolysin synthesis. although many bacillus genes have been identified as sigd regulon, the list may be not be complete. with microarray-based systemic screening, we found a set of genes downregulated in the sigd knockout mutant of the plant growth-promoting rhizobacterium b. amyloliquefaciens subsp. plantarum fzb42. eight genes (appa, bls ...201627797724
antimicrobial peptide isolated from bacillus amyloliquefaciens k14 revitalizes its use in combinatorial drug therapy.the present study was performed to evaluate the antibacterial activities of an antimicrobial peptide (cspk14) and the synergies thereof with β-lactams against vancomycin-resistant staphylococcus aureus (vrsa) and enterococci (vre). our strain was isolated from fermented food (kimchi), which is 99.79 % homologous with bacillus amyloliquefaciens subsp. plantarum fzb42(t). cspk14 was purified to homogeneity by diammonium sulfate precipitation, concentration, dialysis, and followed by two-stage chro ...201727787755
comparative digital gene expression analysis of the arabidopsis response to volatiles emitted by bacillus amyloliquefaciens.some plant growth-promoting rhizobacteria (pgpr) regulated plant growth and elicited plant basal immunity by volatiles. the response mechanism to the bacillus amyloliquefaciens volatiles in plant has not been well studied. we conducted global gene expression profiling in arabidopsis after treatment with bacillus amyloliquefaciens fzb42 volatiles by illumina digital gene expression (dge) profiling of different growth stages (seedling and mature) and tissues (leaves and roots). compared with the c ...201627513952
comparative transcriptome analysis of the biocontrol strain bacillus amyloliquefaciens fzb42 as response to biofilm formation analyzed by rna sequencing.the strain bacillus amyloliquefaciens fzb42 is a plant growth promoting rhizobacterium (pgpr) and biocontrol agent known to keep infections of lettuce (lactuca sativa) by the phytopathogen rhizoctonia solani down. several mechanisms, including the production of secondary metabolites possessing antimicrobial properties and induction of the host plant's systemic resistance (isr), were proposed to explain the biocontrol effect of the strain. b. amyloliquefaciens fzb42 is able to form plaques (biofi ...201627312701
the new flagella-associated collagen-like proteins clpb and clpc of bacillus amyloliquefaciens fzb42 are involved in bacterial motility.collagen-like proteins (clps) share the distinctive gly-x-thr repeating amino acid sequence of animal collagens, and contain n- and c-terminal domain making a collagen-like structure in bacillus amyloliquefaciens fzb42, a plant growth-promoting rhizobacterium. our previous study demonstrated that clps play important roles in biofilm construction and adherence to the surfaces on plant roots. however, bacterial localization of the clps remains unclear. here, disrupted strains on all four clp genes ...201626856450
genome sequence of antibiotic-producing bacillus amyloliquefaciens strain kctc 13012.we report the 4.0-mb draft genome sequence of bacillus amyloliquefaciens (syn. bacillus velezensis) kctc 13012, which exhibits a broad spectrum of antagonistic activity against bacteria and fungi and promotes plant growth as well. the genome contains an array of biosynthetic gene clusters for secondary metabolites that are comparable to those in bacillus amyloliquefaciens subsp. plantarum fzb42(t).201526430040
single bacteria movement tracking by online microscopy--a proof of concept study.in this technical report we demonstrate a low-cost online unit allowing movement tracking of flagellated bacteria on a single-cell level during fermentation processes. the system's ability to distinguish different metabolic states (viability) of bacteria by movement velocity was investigated. a flow-through cuvette with automatically adjustable layer thickness was developed. the cuvette can be used with most commercially available laboratory microscopes equipped with 40× amplification and a digi ...201525849813
bacilysin overproduction in bacillus amyloliquefaciens fzb42 markerless derivative strains fzbrep and fzbspa enhances antibacterial activity.bacillus amyloliquefaciens strains fzbrep and fzbspa were derived from the wild-type fzb42 by replacement of the native bacilysin operon promoter with constitutive promoters p repb and p spac from plasmids pmk3 and ploss, respectively. these strains contained two antibiotic resistance genes, and markerless strains were constructed by deleting the chloramphenicol resistance cassette and promoter region bordered by two lox sites (lox71 and lox66) using cre recombinase expressed from the temperatur ...201525472439
influence of root exudates on the extracellular proteome of the plant growth-promoting bacterium bacillus amyloliquefaciens fzb42.proteins secreted by bacillus amyloliquefaciens fzb42, a root-associated plant growth-promoting rhizobacterium, are thought to play an important role in the establishment of beneficial interactions with plants. to investigate the possible role of proteins in this process, extracellular proteome maps of b. amyloliquefaciens fzb42 during the late exponential and stationary growth phases were generated using 2d gel electrophoresis. out of the 121 proteins identified by maldi-tof ms, 61 were predict ...201525355936
draft genome sequence of plant growth-promoting bacillus amyloliquefaciens strain w2 associated with crocus sativus (saffron).bacillus sp. strain w2 is a plant growth-promoting rhizobacterium isolated from saffron fields of kashmir, india. here, we report the draft genome sequence (3.9 mb) of bacillus amyloliquefaciens strain w2 having 65 contigs (3, 997, 511 bp), 4,163 coding sequences, and an average 46.45% gc content. despite the 99% identity of the 16s rrna gene with that of bacillus amyloliquefaciens subsp. plantarum fzb42, the genome comparison revealed that only 48.7% of the w2 genome has homology with that of f ...201425212614
[establishment and application of efficient nematicidal screening model in bacillus amyloliquefaciens].the mechanisms of nematophagous bacteria against nematodes remain unclear, limiting the use of biocontrol bacteria in the agriculture. therefore, we constructed a rapid and efficient screening model to quickly identify new candidate genes involved in nematode infection.201425199258
effect of the strain bacillus amyloliquefaciens fzb42 on the microbial community in the rhizosphere of lettuce under field conditions analyzed by whole metagenome sequencing.application of the plant associated bacterium bacillus amyloliquefaciens fzb42 on lettuce (lactuca sativa) confirmed its capability to promote plant growth and health by reducing disease severity (ds) caused by the phytopathogenic fungus rhizoctonia solani. therefore this strain is commercially applied as an eco-friendly plant protective agent. it is able to produce cyclic lipopeptides (clp) and polyketides featuring antifungal and antibacterial properties. production of these secondary metaboli ...201424904564
auxin secretion by bacillus amyloliquefaciens fzb42 both stimulates root exudation and limits phosphorus uptake in triticum aestivium.the use of auxin-producing rhizosphere bacteria as agricultural products promises increased root production and therefore greater phosphate (pi) uptake. whilst such bacteria promote root production in vitro, the nature of the bacteria-plant interaction in live soil, particularly concerning any effects on nutrient uptake, are not known. this study uses bacillus amyloliquefaciens fzb42, an auxin-producing rhizobacterium, as a dressing on triticum aestivium seeds. it then examines the effects on ro ...201424558978
medium-chain dehydrogenases with new specificity: amino mannitol dehydrogenases on the azasugar biosynthetic pathway.azasugar biosynthesis involves a key dehydrogenase that oxidizes 2-amino-2-deoxy-d-mannitol to the 6-oxo compound. the genes encoding homologous nad-dependent dehydrogenases from bacillus amyloliquefaciens fzb42, b. atrophaeus 1942, and paenibacillus polymyxa sc2 were codon-optimized and expressed in bl21(de3) escherichia coli. relative to the two bacillus enzymes, the enzyme from p. polymyxa proved to have superior catalytic properties with a vmax of 0.095 ± 0.002 µmol/min/mg, 59-fold higher th ...201424354768
the highly modified microcin peptide plantazolicin is associated with nematicidal activity of bacillus amyloliquefaciens fzb42.bacillus amyloliquefaciens fzb42 has been shown to stimulate plant growth and to suppress the growth of plant pathogenic organisms including nematodes. however, the mechanism underlying its effect against nematodes remains unknown. in this study, we screened a random mutant library of b. amyloliquefaciens fzb42 generated by the mariner transposon tnylb-1 and identified a mutant strain f5 with attenuated nematicidal activity. reversible polymerase chain reaction revealed that three candidate gene ...201324085393
leaf microbiota of strawberries as affected by biological control agents.the increasing use of biological control agents (bcas) against botrytis cinerea in strawberry raises the question of whether there are any undesirable impacts of foliar applications of bcas on nontarget microorganisms in the phyllosphere. therefore, our objective was to investigate this issue within a field study. strawberry plants were repeatedly sprayed with three bcas-namely, rhizovital 42 fl. (bacillus amyloliquefaciens fzb42), trianum-p (trichoderma harzianum t22), and naturalis (beauveria ...201324020904
effects of bacillus amyloliquefaciens fzb42 on lettuce growth and health under pathogen pressure and its impact on the rhizosphere bacterial community.the soil-borne pathogen rhizoctonia solani is responsible for crop losses on a wide range of important crops worldwide. the lack of effective control strategies and the increasing demand for organically grown food has stimulated research on biological control. the aim of the present study was to evaluate the rhizosphere competence of the commercially available inoculant bacillus amyloliquefaciens fzb42 on lettuce growth and health together with its impact on the indigenous rhizosphere bacterial ...201323935892
bacterial traits involved in colonization of arabidopsis thaliana roots by bacillus amyloliquefaciens fzb42.colonization studies previously performed with a green-fluorescent-protein, gfp, labeled derivative of bacillus amyloliquefaciens fzb42 revealed that the bacterium behaved different in colonizing surfaces of plant roots of different species (fan et al., 2012). in order to extend these studies and to elucidate which genes are crucial for root colonization, we applied targeted mutant strains to arabidopsis seedlings. the fates of root colonization in mutant strains impaired in synthesis of alterna ...201325288929
two-component response regulator degu controls the expression of bacilysin in plant-growth-promoting bacterium bacillus amyloliquefaciens fzb42.the plant-growth-promoting-rhizobacteria bacillus amyloliquefaciens fzb42 possess an enormous potential to synthesize a wide range of antimicrobial, antiviral and nematocidal compounds. one of them, the dipeptide antibiotic bacilysin, is synthesized by fzb42 during exponential growth. here, we have demonstrated that bacilysin is positively regulated by the two-component response regulator degu at the transcriptional level. in addition, scoc (hpr), a transition state regulator, negatively control ...201222677979
gram-positive rhizobacterium bacillus amyloliquefaciens fzb42 colonizes three types of plants in different patterns.the colonization of three types of different plants, zea mays, arabidopsis thaliana, and lemna minor, by gfp-labeled gram-positive rhizobacterium bacillus amyloliquefaciens fzb42 was studied in gnotobiotic systems using confocal laser scanning microscopy and electron microscopy. it was demonstrated that fzb42 was able to colonize all the plants. on one hand, similar to some gram-negative rhizobacteria like pseudomonas, fzb42 favored the areas such as the concavities in root surfaces and the junc ...201222367935
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