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[the investigation on plant resources and introduction of dioscorea zingiberensis wright (author's transl)]. 19826212148
[progress and prospect of botancial research in dioscorea zingiberensis].this paper is a summary of the progress of botanical research in dioscorea zingiberensis. it contains biological characteristic, genetic diversity, growth circumstance, breeding, cultivating, tissue and cell culture of d. zingiberensis. according to the fact of market demanding and resources protection, the writers propose that some work, including establishing gene bank, resources appraise, employing advanced technology to utlize the resources and making the cultivated standard, should be taken ...200516499012
[selection of the microorganism for dioscin hydrolyze].to select the microorganism which can hydrolyze dioscin to diosgenin.200718074832
acute toxicity and sub-chronic toxicity of steroidal saponins from dioscorea zingiberensis c.h.wright in rodents.steroidal saponins from dioscorea zingiberensis are widely used in china for curing cardiovascular diseases. however, there was little toxicological information available on them.200919735710
enhancement of diosgenin production in dioscorea zingiberensis cell culture by oligosaccharide elicitor from its endophytic fungus fusarium oxysporum dzf17.diosgenin accumulation in cell suspension cultures of dioscorea zingiberensis c. h. wright was enhanced by treatment with saccharide elicitors from its endophytic fungus fusarium oxysporum isolate dzf17. the crude oligosaccharide was prepared by partial acid hydrolysis of the isolated dzf17 fungal cell wall fragments. optimal elicitation of diosgenin production by the isolated dzf17 oligosaccharide in cell suspension culture was achieved when the oligosaccharide was added to the medium at a conc ...200919967973
beauvericin from the endophytic fungus, fusarium redolens, isolated from dioscorea zingiberensis and its antibacterial activity.from the fungal endophyte, fusarium redolens dzf2, isolated from rhizomes of the chinese medicinal plant dioscorea zingiberensis, beauvericin was obtained by tlc in combination with bioautographic antibacterial assay. the compound was identified by spectroscopic and physicochemical means. the median effective inhibitory concentration (ic50) values of beauvericin against six test bacteria (bacillus subtilis, staphylococcus haemolyticus, pseudomonas lachrymans, agrobacterium tumefaciens, escherich ...201020521553
anti-thrombotic activity and chemical characterization of steroidal saponins from dioscorea zingiberensis c.h. wright.steroidal saponins have long attracted scientific attention, due to their structural diversity and significant biological activities. total steroidal saponins (tss) extracted from the rhizomes of dioscorea zingiberensis c.h. wright (dzw) constitute an effective treatment for cardiovascular disease. however, the active constituents contained in dzw rhizomes and their pharmacological properties are not fully understood. the aim of this work is to determine and quantify the active constituents in d ...201020659537
[protective effect of extractive of dioscorea zingiberensis against ischemia/reperfusion injury in cultured neonatal rat cardiomyocytes].to define the structure of a water-soluble compound (sy-a) extracted from dioscorea zingiberensis and investigate its protective effect against the neonatal rat cardiomyocytes ischemia/reperfusion (i/r) injured.201020681310
anthelmintic activity of steroidal saponins from dioscorea zingiberensis c. h. wright against dactylogyrus intermedius (monogenea) in goldfish (carassius auratus).dactylogyrus intermedius is one of the most pathogenic monogenean parasites on the gills of captive fish and can cause serious problem in aquaculture. to attempt controlling this parasite and explore novel potential antiparasitic agents, the present study was designed to investigate the anthelmintic activity of dioscorea zingiberensis c. h. wright against d. intermedius in goldfish under in vivo conditions. bioactivity-guided fractionation and isolation of the compounds responsible for anthelmin ...201020689967
enhancement of diepoxin ζ production by yeast extract and its fractions in liquid culture of berkleasmium-like endophytic fungus dzf12 from dioscorea zingiberensis.this study was to examine the effects of yeast extract (ye) and its fractions (ye1 and ye2) on the growth and diepoxin ζ (a spirobisnaphthalene with a diversity of bioactivities) production in liquid culture of berkleasmium-like endophytic fungus dzf12 from dioscorea zingiberensis. when ye was applied to the liquid medium at 10 g/l on day 3 of culture, the diepoxin ζ production was most effectively enhanced 3.2-fold (378.70 mg/l versus 120.09 mg/l in control) after another 10 days culture. feedi ...201121248666
anti-thrombosis effect of diosgenin extract from dioscorea zingiberensis c.h. wright in vitro and in vivo.thrombus formation in blood vessel plays an important role in the pathogenesis and progression of atherosclerosis and cardiovascular diseases. extract of dioscorea zingiberensis c.h. wright (d. zingiberensis) is demonstrated to posses activities for curing cardiovascular diseases such as coronary heart disease and angina pectoris. however, there were few studies on anti-thrombosis activity of it. we investigated the anti-thrombosis effect of diosgenin from d. zingiberensis (dio) in vitro and in ...201021036572
a novel β-glucosidase from aspergillus fumigates releases diosgenin from spirostanosides of dioscorea zingiberensis c. h. wright (dzw).a β-glucosidase effectively releasing diosgenin from spirostanosides of dioscorea zingiberensis c. h. wright (dzw), named afg, was purified from a strain of aspergillus fumigates. the molecular weight of afg was 113 kda. analysis of protein fragments by esi-q-tof indicated that afg was a β-glucosidase. the circular dichroism spectrum suggested that the main secondary structure of afg in milli-q water was α-helixes. atomic force microscopy revealed that it was a globular protein. afg maintained h ...201122805852
fungal endophytes from dioscorea zingiberensis rhizomes and their antibacterial activity.the aim of the study was to isolate and characterize the endophytic fungi from the rhizomes of the chinese traditional medicinal plant dioscorea zingiberensis and to detect their antibacterial activities.200818086193
improved production of transgenic dioscorea zingiberensis (dioscoreaceae) by agrobacterium tumefaciens-mediated transformation.the establishment of high-efficiency agrobacterium-mediated transformation techniques could improve the production of dioscorea zingiberensis, a medicinal species with a high diosgenin content. we co-cultivated embryogenic calli induced from mature seeds with a. tumefaciens strain eha105. a binary vector, pcambia1381, which contains the gfp and hpt genes under the control of the ubiquitin promoter and the camv 35s promoter, respectively, was used for transformation. pre-culture, basic medium, ac ...201222370891
[antagonism against beauveria bassiana by lipopeptide metabolites produced by entophyte bacillus amyloliquefaciens strain swb16].we screened bacterial strains that have strong antagonism against beauveria bassiana, an important pathogen of silkworm industry, and detected the antagonistic activity of lipopeptide metabolites.201425252459
Enhancement of Diosgenin Production in Dioscorea zingiberensis Cell Cultures by Oligosaccharides from Its Endophytic Fungus Fusarium oxysporum Dzf17.The effects of the oligosaccharides from the endophytic fungus Fusarium oxysporum Dzf17 as elicitors on diosgenin production in cell suspension cultures of its host Dioscorea zingiberensis were investigated. Three oligosaccharides, DP4, DP7 and DP10, were purified from the oligosaccharide fractions DP2-5, DP5-8 and DP8-12, respectively, which were prepared from the water-extracted mycelial polysaccharide of the endophytic fungus F. oxysporum Dzf17. When the cell cultures were treated with fracti ...201122183887
isolation and characterization of a furfural-degrading bacterium bacillus cereus sp. strain ds1.furfural was found to be the main organic pollutant in the wastewater coming from the diosgenin factory. this substance is derived from acidic pentosan in dioscorea zingiberensis and is also found in a variety of agricultural byproducts, including corncobs, oat, wheat bran, and sawdust. it is regarded as a toxicant and an inhibitor to the growth of microorganism in both sewage disposal and biological fermentation. a furfural-degrading strain (ds1) was isolated from activated sludge of wastewater ...201525274411
novel method utilizing microbial treatment for cleaner production of diosgenin from dioscorea zingiberensis c.h. wright (dzw).a novel method utilizing microbial treatment for cleaner production of diosgenin from dioscorea zingiberensis c.h. wright (dzw) was presented. a new bacillus pumilus hr19, which has the great ability to secrete pectinase, was screened and applied in the microbial treatment. low-pressure steam expansion pretreatment (lsep) was employed in advance to assist microbial treatment efficiently in releasing saponins, which are the precursors of diosgenin. compared with the traditional process of acid hy ...201323973974
molecular cloning and differential expression analysis of a squalene synthase gene from dioscorea zingiberensis, an important pharmaceutical plant.diosgenin is a steroid derived from cholesterol in plants and used as a typical initial intermediate for synthesis of numerous steroidal drugs in the world. commercially, this compound is extracted mainly from the rhizomes or tubers of some dioscorea species. squalene synthase (sqs: ec 2.5.1.21) catalyzes the condensation of two molecules of farnesyl diphosphate to form squalene, the first committed step for biosynthesis of plant sterols including cholesterol, and is thought to play an important ...201424996285
deltonin, a steroidal saponin, inhibits colon cancer cell growth in vitro and tumor growth in vivo via induction of apoptosis and antiangiogenesis.deltonin, a steroidal saponin, isolated from dioscorea zingiberensis wright (dzw), has shown high-cytotoxic activity in cancer cells. however, its mechanisms and in vivo anti-cancer effects remain unknown. in the present study, we evaluated the effects and explored the anti-tumor mechanisms of deltonin on a panel of colon cancer cell lines and in a mouse model of murine colon cancer c26. deltonin had more cytotoxic effect on c26 cells than 5-fluorouracil had, promoting dramatic g2-m phase arrest ...201121471712
bioactive spirobisnaphthalenes from the endophytic fungus berkleasmium sp.nine new spirobisnaphthalenes, palmarumycins b1-b9 (1-9), along with 13 known compounds (10-22), were isolated from cultures of the fungus berkleasmium sp., an endophyte isolated from the medicinal plant dioscorea zingiberensis c. h. wright. the structures of the new compounds were elucidated by analysis of the 1d and 2d nmr and hresims spectra and by comparison with known compounds. compounds 7-9 contain an uncommon 2,3-dihydro-1h-inden-1-one unit. all isolated compounds were evaluated for thei ...201425237727
biotransformation of steriodal saponins in dioscorea zingiberensis c. h. wright to diosgenin by trichoderma harzianum.diosgenin is an important starting material in the steroidal hormone industry. traditionally, diosgenin is mainly produced by acid hydrolysis of dioscorea zingiberensis c. h. wright (dzw) tubers. this method yields numerous byproducts that can cause serious pollution. in this study, diosgenin was obtained by biotransformation of steroidal saponins in dzw afforded by trichoderma harzianum cgmcc 2979. the medium was optimized for maximum diosgenin production. the addition of phosphate buffer, surf ...201019578845
larvicidal spirobisnaphthalenes from the endophytic fungus berkleasmium sp. against aedes albopictus.in our screening programme for new agrochemicals from endophytic fungi, the ethyl acetate extract of an endophytic berkleasmium sp. isolated from the medicinal plant dioscorea zingiberensis was found to possess strong larvicidal activity against the asian tiger mosquito, aedes albopictus.201626171851
extraction optimization of water-extracted mycelial polysaccharide from endophytic fungus fusarium oxysporum dzf17 by response surface methodology.water-extracted mycelial polysaccharide (wps) from the endophytic fungus fusarium oxysporum dzf17 isolated from dioscorea zingiberensis was found to be an efficient elicitor to enhance diosgenin accumulation in d. zingigerensis cultures, and also demonstrated antioxidant activity. in this study, response surface methodology (rsm) was employed to optimize the extraction process of wps from f. oxysporum dzf17 using box-behnken design (bbd). the ranges of the factors investigated were 1-3 h for ext ...201222754306
[screening and condition optimization of a strain for efficiently biotransformation of saponins in dioscorea zingiberensis into diosgenin].diosgenin is an important raw material in steroid hormone and widely used in pharmaceutical industry. the traditional method for diosgenin production is acidolysis, which causes serious pollution. in order to obtain a cleaner and more efficient approach of diosgenin production, a strain of gibberella intermedia wx12 (the sexual stage of fusarium proliferatum) was screened from the strains deposited in our laboratory. this strain converted saponins in dioscorea zingiberensis c.h. wright (dzw) int ...201324063243
phenolic compounds isolated from dioscorea zingiberensis protect against pancreatic acinar cells necrosis induced by sodium taurocholate.one new bibenzyl (1) and one new phenanthrene (2), together with two known bibenzyls (3-4) and four known diarylheptanoids (5-8) were isolated from the rhizomes of dioscorea zingiberensis. the structures of 1-2 were elucidated by spectroscopic methods including 1d and 2d nmr. phenols 1-8 were evaluated for their anti-pancreatitic activities on sodium taurocholate (nat)-induced pancreatic acinars necrosis. notably, 0.5mm of compound 6 exhibited comparable inhibitory effect with 5mm of caffeine. f ...201728228364
one compound of saponins from disocorea zingiberensis protected against experimental acute pancreatitis by preventing mitochondria-mediated necrosis.acute pancreatitis (ap) is a painful inflammatory disorder of the exocrine pancreas, ranking as the most common gastrointestinal reasons for hospitalization with no specific therapy currently. diosgenyl saponins extracted from natural products and diosgenin or its derivatives have been shown to exert anti-inflammatory effects in various diseases. however, the therapeutic effects of diosgenyl saponins from dioscorea zingiberensis c. h. wright in ap have not yet been determined. five compounds wer ...201627779235
comparative studies on performance of ccc and preparative rp-hplc in separation and purification of steroid saponins from dioscorea zingiberensis c.h.wright.steroid saponins from dioscorea zingiberensis c.h.wright were separated for the first time using two chromatographic methods for comparison: counter-current chromatography (ccc) coupled with evaporative light scattering detector (elsd) and preparative reversed phase high-performance liquid chromatography (rp-hplc) with an ultraviolet detector. ethyl acetate-n-butanol-methanol-water (4:1:2:4, v/v) was chosen as the two-phase solvent system for ccc, while the acetonitrile-water (25:75 for the firs ...201526726306
9,10-dihydrophenanthrene derivatives and one 1,4-anthraquinone firstly isolated from dioscorea zingiberensis c. h. wright and their biological activities.two new phenanthrene derivatives, 2,5,7-trimethoxy-9,10-dihydrophenanthrene-1,4-dione (1) and 2,5,6-trihydroxy-3,4-dimethoxy-9,10-dihydrophenanthrene (3), one new anthracenedione, 2,5,7-trimethoxyanthracene-1,4-dione (2), together with two known 9,10-dihydrophenanthrenes (4-5) were isolated from the rhizomes of dioscorea zingiberensis c. h. wright. the structures of these new compounds were established based on extensive nmr spectroscopy. several isolated compounds were evaluated for the inhibit ...201626656408
enhancement of palmarumycin c12 and c13 production by the endophytic fungus berkleasmium sp. dzf12 in an aqueous-organic solvent system.the endophytic fungus berkleasmium sp. dzf12, isolated from dioscorea zingiberensis, was found to produce palmarumycins c12 and c13 which possess a great variety of biological activities. seven biocompatible water-immiscible organic solvents including n-dodecane, n-hexadecane, 1-hexadecene, liquid paraffin, dibutyl phthalate, butyl oleate and oleic acid were evaluated to improve palmarumycins c12 and c13 production in suspension culture of berkleasmium sp. dzf12. among the chosen solvents both b ...201526569213
microwave-assisted extraction and a new determination method for total steroid saponins from dioscorea zingiberensis c.h. wright.an efficient microwave-assisted extraction (mae) technique was applied to isolate total steroid saponins from dioscorea zingiberensis c.h. wright (dzw). the optimal extracting conditions were established as 75% ethanol as solvent, ratio of solid/liquid 1:20 (g/ml), temperature 75 °c, irradiation power 600 w and three extraction cycles of 6 min each. scanning electron microscopy (sem) images of dzw processed by four different extractions provided visual evidence of the disruption effect on dzw. d ...201526432686
in situ high-valued utilization and transformation of sugars from dioscorea zingiberensis c.h. wright for clean production of diosgenin.the industrial production of diosgenin in china generates a large amount of high-sugar wastes with low bioavailability, which causes serious pollution to the environment. in this study, a new clean and efficient process for the production of diosgenin was developed using sugars through in situ high-valued transformation. the sugar mixture from dioscorea zingiberensis c.h. wright contained abundant beneficial components. nine typical microorganisms that produced intracellular products were evalua ...201526299979
in situ pressurized biphase acid hydrolysis, a promising approach to produce bioactive diosgenin from the tubers of dioscorea zingiberensis.the tubers of dioscorea zingiberensis, is the most favorable plant material for the production of diosgenin, an important bioactive steroidal sapogenin and requisite precursor of cortin, contraceptive and sex hormone, which is the only desired product after steroidal saponins from the tubers are hydrolyzed.201726246743
[preparation technique of tetraploid of dioscorea zingiberensis synthetic seed based on embryogenic callus].to provide technical support for industrialization promotion of tetraploid of dioscorea zingiberensis, the manufacturing method for synthetic seeds of tetraploid of dioscorea zingiberensis was established and the correlated influential factors were studied.201526214862
the complete chloroplast genome sequence of dioscorea zingiberensis (dioscoreceae).dioscorea zingiberensis (dioscoreceae) is an important medicinal plant endemic to china. here, its chloroplast genome sequence is reconstructed from the whole-genome illumina sequencing data. the circular genome is 153,970 bp in length, and comprises a pair of inverted repeat (ir) regions of 25,491 bp each, a large single-copy (lsc) region of 83,950 bp and a small single-copy (ssc) region of 19,038 bp. the chloroplast genome contains 132 genes, including 86 protein-coding genes (79 pcg species), ...201626066025
evaluation of different pretreatments on microbial transformation of saponins in dioscorea zingiberensis for diosgenin production.in order to evaluate the effects of different pretreatments on microbial transformation of saponins in dioscorea zingiberensis (dzw), various methods have been systematically studied on a large scale. five pretreatments, including physical separation, catalytic solvent extraction, ultrasonic fermentation, complex enzymatic hydrolyzation and enzymatic saccharification, were performed on dzw. compared with other methods, complex enzymatic hydrolyzation significantly improved the efficiency of micr ...201426019558
[advances in study of dioscin--a natural product].dioscin, a typical saponin, is widely present in the family of dioscoreaceae, liliaceae, caryophyllaceae and rosaceae, especially in dioscoreaceae, including discorea nipponica makino, dioscorea zingiberensis c. h. wright and dioscorea panthaica prain et burkill. traditional chinese medicine reported that dioscin plays a role in expectorant, relaxing the muscles and stimulating the blood circulation, aiding digestion and diuresis. with the development of science and technology in recent years, s ...201525993784
direct biotransformation of dioscin into diosgenin in rhizome of dioscorea zingiberensis by penicillium dioscin.diosgenin is an important precursor for synthesis of more than 200 steroidal hormone medicines. rhizome of dioscorea zingiberensis c. h. wright (rdz) contained the highest content of diosgenin in dioscorea plant species. diosgenin is traditionally extracted by acid hydrolysis from rdz. however, the acid hydrolysis process produces massive wastewater which caused serious environment pollution. in this study, diosgenin extraction by direct biotransformation with penicillium dioscin was investigate ...201525805907
uplc-qtof-ms identification of metabolites in rat biosamples after oral administration of dioscorea saponins: a comparative study.among the 49 species of the genus dioscorea distributed in china, dioscorea nipponica makino (dn), dioscorea panthaica prain et burkill (dp), and dioscorea zingiberensis c. h. wright (dz) possess more or less similar traditional therapeutic actions, such as activating blood, relieving pain, and dispersing swelling; they have been used as folk medicine in china since 1950s. the modern pharmaceutical industry has developed these three species as herbal medicines that have been used for decades for ...201525698242
[study on steroidal saponins from dioscorea zingiberensis and their platelet aggregation activities].using the absorbent resin, silica gel and ods column chromatography as well as semi-preparative hplc, ten compounds were isolated from 70% ethanol extract of tubers of dioscorea zingiberensis c. h. wright, and their structures were elucidated as trigoneoside xiiia (1), parvifloside (2), trigoneoside iva (3), deltoside (4), protobioside (5), lilioglycoside k (6), zingiberensis newsaponin i (7), deltonin (8), prosapogenin a of dioscin (9), and trillin (10) on the basis of nmr and ms spectral data ...201425612440
steroidal saponins obtained by biotransformation of total furostanol glycosides from dioscorea zingiberensis with absidia coerulea.five new steroidal saponins (1-5) were isolated from the fermentation broth of total furostanol glycosides from tubers of dioscorea zingiberensis c.h. wright incubated with a fungal, absidia coerulea as 3.3389, along with known saponins, zingiberensis new saponin (6), deltonin (7), prosapogenin a of dioscin (8), and protobioside (9), and their structures were established by nmr spectroscopy and mass spectrometry as well as by comparison with previously reported spectral data in the literatures. ...201525498025
neuroprotection of total steroid saponins from dioscorea zingiberensis against transient focal cerebral ischemia-reperfusion injury in rats via anti-inflammatory and antiapoptotic effects.total steroid saponins isolated from dioscorea zingiberensis have shown a variety of beneficial bioactivities. however, there are no reports about their neuroprotective effects, until now. therefore, in the present study, we explored the neuroprotective effects of the total steroid saponins from d. zingiberensis on rats against transient focal cerebral ischemia-reperfusion and their underlying mechanisms. healthy adult sprague-dawley rats were randomly assigned to six groups. after pretreatment ...201425340467
simultaneous quantification of five steroid saponins from dioscorea zingiberensis c.h. wright in rat plasma by hplc-ms/ms and its application to the pharmacokinetic studies.a simple, reliable and sensitive high-performance liquid chromatography tandem mass spectrometry method (hplc-ms/ms) was established for simultaneous analyses of the following 5 steroid saponins in rat plasma after the single dose administration of total steroid saponins extracted from the rhizome of dioscorea zingiberensis c.h. wright for the first time. protodioscin, huangjiangsu a, zingiberensis new saponin, dioscin, and gracillin were quantified using ginsenoside rb1 as the internal standard ...201525201262
[genetic stability study on autotetraploid plant of dioscorea zingiberensis].to study the genetic stability of autotetraploid plant of dioscorea zingiberensis.201425174097
therapeutic effects of total steroid saponin extracts from the rhizome of dioscorea zingiberensis c.h.wright in freund's complete adjuvant induced arthritis in rats.the aim of our present study is to explore the anti-arthritic potential effect of total steroid saponins (tssns) extracted from the rhizome of dioscorea zingiberensis c.h.wright (dzw) and to investigate the underlying mechanisms. this work was performed using adjuvant-induced arthritis (aia) rats in vivo and lipopolysaccharide (lps) simulated 264.7 macrophage cells in vitro. in aia-induced arthritic rats, tssn significantly alleviated the arthritic progression through evaluating arthritic score, ...201425066758
neuroprotective effects of total steroid saponins on cerebral ischemia injuries in an animal model of focal ischemia/reperfusion.total steroid saponins isolated from dioscorea zingiberensis are a unique traditional chinese medicine known for its potential usage in various types of diseases. however, there is little evidence about its neuroprotective effect in transient focal ischemia-reperfusion cerebral injury. therefore, the current study was carried out to investigate the effect of total steroid saponins on neuroprotection and its potential mechanisms in the rat ischemia-reperfusion model by middle cerebral artery occl ...201424963614
dioscin promotes osteoblastic proliferation and differentiation via lrp5 and er pathway in mouse and human osteoblast-like cell lines.dioscin, a typical steroid saponin, is isolated from dioscorea nipponica makino and dioscorea zingiberensis wright. it has estrogenic activity and many studies have also reported that dioscorea plants have an effect in preventing and treating osteoporosis. however, the molecular mechanisms underlying their effect on osteoporosis treatment are poorly understood. therefore, the present study aims to investigate the mechanism (s) by which dioscin promotes osteoblastic proliferation and differentiat ...201424742230
two new steroidal saponins from the rhizomes of dioscorea zingiberensis.to investigate the chemical constituents of dioscorea zingiberensis c. h. wright.201424636066
[study on hplc-elsd fingerprint of total steroid saponins in herbs of dioscorea zingiberensis].to establish a hplc-elsd fingerprint for total steroid saponins in herbs of dioscorea zingiberensis. welchrom c,8 (4. 6 mm x 250 mm,5 microm) chromatographic column was adopted and eluted with the mobile phase of acetonitrile (a)-water (b) at the flow rate of 1.0 ml min-1. the column temperature was room temperature. the elsd conditions were as follows: the temperature of drift tube was 90.0 degreec, the flow rate of carrier gas (n2) was 2. 8 l min-1, and the injection volume was 10 microl. afte ...201324422399
quality control and identification of steroid saponins from dioscorea zingiberensis c. h. wright by fingerprint with hplc-elsd and hplc-esi-quadrupole/time-of-fight tandem mass spectrometry.in this study, a fingerprint of steroid saponins, the major bioactive constituents in the crude extracts from dioscorea zingiberensis c. h. wright (dzw), has been established for the first time by combined use of the following two methods: high-performance liquid chromatography coupled with evaporative light scattering detector (hplc-elsd) and the simultaneous characterization of the steroid saponins by high-performance liquid chromatography coupled with electrospray ionization-mass spectrometry ...201424418811
[study on inducing embryogenic callus of dioscorea zingiberensis by orthogonal design].to select the suitable medium to induce embryogenic callus of dioscorea zingiberensis.201324218956
preparative separation of spirobisnaphthalenes from endophytic fungus berkleasmium sp. dzf12 by high-speed counter-current chromatography.high-speed counter-current chromatography (hsccc) was applied for the first time for the preparative separation of spirobisnaphthalenes from a crude extract of the endophytic fungus berkleasmium sp. dzf12, associated with the medicinal plant dioscorea zingiberensis. six spirobisnaphthalenes were successfully separated by hsccc with a two-phase solvent system composed of n-hexane-chloroform-methanol-water (1.5:3.0:2.5:2.0, v/v). about 18.0 mg of diepoxin κ (1), 245.7 mg of palmarumycin c13 (2), 4 ...201324135943
development of polymorphic microsatellite markers for dioscorea zingiberensis and cross-amplification in other dioscorea species.dioscorea zingiberensis c.h. wright (dioscoreaceae) is an endemic species in central and southwestern china. in order to study the genetic diversity and population structure of this species, 19 novel polymorphic microsatellite loci were developed using a dual-suppression pcr technique. the number of alleles per locus ranged from 3 to 21, with an average of 9.53. all the markers showed high transferability in cross-species amplification in other species of sect. stenophora.201324085440
anti-thrombosis effect of diosgenyl saponins in vitro and in vivo.thrombosis in coronary or cerebral arteries is the major cause of morbidity and mortality worldwide. diosgenin and total steroidal saponins extracted from the rhizome of dioscorea zingiberensis c.h. wright are demonstrated to have anti-thrombotic activity. however, few studies describe the anti-thrombotic activity of the diosgenyl saponin monomer. in the present study, a simple and convenient method for the preparation of a new disaccharide saponin, diosgenyl β-d-galactopyranosyl-(1→4)-β-d-gluco ...201323891646
investigation on the mechanisms for biotransformation of saponins to diosgenin.in order to improve the efficiency of biotransformation of saponins in dioscorea zingiberensis to diosgenin, a new enzymatic model was developed to investigate the mechanism of the metabolic systems. four main saponin hydrolases (e1, e2, e3 and e4) were purified from trichoderma reesei. using progracillin as substrate, the enzymatic hydrolysis experiments with e1, e2, e3 and e4 were carried out respectively. saponin concentrations during each biotransformation reaction were constructed with a ki ...201423861042
preparative isolation and purification of five steroid saponins from dioscorea zingiberensis c.h.wright by counter-current chromatography coupled with evaporative light scattering detector.a counter-current chromatography (ccc) method was successfully applied to separate and purify steroid saponins from the traditional chinese medicine dioscorea zingiberensis c.h.wright for the first time. ethyl acetate-n-butanol-methanol-water (4:1:2:4, v/v) was used as the two-phase solvent system, and evaporative light scattering detector (elsd) was used as the detector in this method. the method separated in a single run the following five steroid saponins: 26-o-β-d-glucopyranosyl-(25r)-furost ...201323831486
determination of deltonin in rat plasma by using hplc-ms/ms and the application of this method in pharmacokinetic studies.deltonin is a naturally occurring spirostanol glycoside from dioscorea zingiberensis c.h. wright, which is used in traditional chinese medicine. it exerts strong cytotoxic effect on c26 cells, inhibits c26 derived-tumor growth, and prolongs the survival of tumor-bearing mice after its oral administration, indicating its potential for use as an anti-tumor drug. to investigate the pharmacokinetic profiles of deltonin, a rapid, sensitive, and simplified high-performance liquid chromatography-tandem ...201323747424
rapid quantitative analysis of diosgenin in the tubers of dioscorea zingiberensis c.h. wright by coupling cellulose enzymolysis and two-phase acid hydrolysis in tandem with hplc-uv.a rapid method was developed to quantify diosgenin in rhizoma dioscoreae zingiberensis. for the first time, sample solution was prepared by coupling pretreatment of raw material in cellulase and two-phase acid hydrolysis. after reconstitution, analysis was carried out on a c18 column, at 30°c, with acetonitrile and water (70:30, v/v) as mobile phase with flow rate of 1.0 ml min(- 1). detection was carried out at 202 nm. good linearity (r(2) = 0.9998) was established between concentration of anal ...201323672189
preparation and evaluation of diosgenin nanocrystals to improve oral bioavailability.diosgenin (dsg), a well-known steroid sapogenin derived from dioscorea nipponica makino and dioscorea zingiberensis wright, has a variety of bioactivities. however, it shows low oral bioavailability due to poor aqueous solubility and strong hydrophobicity. the present study aimed to develop dsg nanocrystals to increase the dissolution and then improve the oral bioavailability and biopharmaceutical properties of dsg. dsg nanocrystals were prepared by the media milling method using a combination o ...201627995466
low pressure steam expansion pretreatment as a competitive approach to improve diosgenin yield and the production of fermentable sugar from dioscorea zingiberensis c.h. wright.development of efficient pretreatment methods which can disrupt the peripheral lignocellulose and even the parenchyma cells is of great importance for production of diosgenin from turmeric rhizomes. it was found that low pressure steam expansion pretreatment (lsep) could improve the diosgenin yield by more than 40% compared with the case without pretreatment, while simultaneously increasing the production of fermentable sugar by 27.37%. furthermore, little inhibitory compounds were produced in l ...201626845219
deltonin induced both apoptosis and autophagy in head and neck squamous carcinoma fadu cell.for decades, despite the advancement of medical science, the prognosis of head and neck squamous cell carcinoma (hnscc), has not improved. deltonin is one of the major active components of dioscorea zingiberensis wright that has been used for anthrax, rheumatic heart disease, rheumatoid arthritis etc. by employing hnscc fadu cell and normal human epidermal keratinocyte, we investigate deltonin efficacy and associated mechanism in both cell culture and nude mice xenografts. deltonin treatment sel ...201525866222
deltonin induces apoptosis in mda‑mb‑231 human breast cancer cells via reactive oxygen species‑mediated mitochondrial dysfunction and erk/akt signaling pathways.deltonin, a steroidal saponin isolated from dioscorea zingiberensis wright, exhibits high cytotoxic activity in cancer cells. in the present study, the effects of deltonin on cell proliferation and apoptosis were evaluated in the mda‑mb‑231 human breast carcinoma cell line. following treatment with deltonin, the viability of mda‑mb‑231 cells was analyzed using mtt assay and apoptosis, mitochondrial membrane potential (∆ψm) alternation and intracellular reactive oxygen species (ros) generation wa ...201323314115
diosgenin down-regulates nf-κb p65/p50 and p38mapk pathways and attenuates acute lung injury induced by lipopolysaccharide in mice.diosgenin (dio), a major active component of steroidal sapogenin of the traditional chinese herb dioscorea zingiberensis c.h.wright, shows various activities including anti-inflammatory, anti-thrombotic activities, anti-cancer properties etc. in the present study, we found that diosgenin significantly suppressed the phosphorylation of lung nf-κb p50/p65 and mapk/p38 in lipopolysaccharide (lps)-induced acute lung injury (ali) in mice, when given orally at doses of 0.1, 1.0 and 10mg/kg 1h prior to ...201323246979
medium optimization for exopolysaccharide production in liquid culture of endophytic fungus berkleasmium sp. dzf12.berkleasmium sp. dzf12, an endophytic fungus from dioscorea zingiberensis, is a high producer of spirobisnaphthalenes with various bioactivities. the exopolysaccharide (eps) produced by this fungus also shows excellent antioxidant activity. in this study, the experimental designs based on statistics were employed to evaluate and optimize the medium for eps production in liquid culture of berkleasmium sp. dzf12. for increasing eps yield, the concentrations of glucose, peptone, kh(2)po(4), mgso(4) ...201223109862
enhancement of diepoxin ζ production in liquid culture of endophytic fungus berkleasmium sp. dzf12 by polysaccharides from its host plant dioscorea zingiberensis.this study is the first report of the enhancement of diepoxin ζ production in liquid culture of the endophytic fungus berkleasmium sp. dzf12 by the polysaccharides from its host plant dioscorea zingiberensis which serve as elicitors. three polysaccharides, namely water-extracted polysaccharide (wep), sodium hydroxide-extracted polysaccharide and acid-extracted polysaccharide were sequentially prepared from the rhizomes of d. zingiberensis. among them, wep was found to be the most effective elici ...201222805921
cytotoxicity and apoptosis-inducing effect of steroidal saponins from dioscorea zingiberensis wright against cancer cells.steroidal saponins from dioscorea zingiberensis wright (dzw) have shown cytotoxic activity in cancer cells. in this study, we isolated and identified seven steroidal saponins from the rhizomes of dzw: diosgenin, trillin, diosgenin diglucoside, deltonin, zingiberensis saponin (zs), protodeltonin and parvifloside. our results showed that these seven compounds inhibited the proliferation of a panel of established human and murine cancer cell lines in vitro. zs had more cytotoxic effect than the oth ...201222575181
enhancement of palmarumycin c12 and c13 production in liquid culture of the endophytic fungus berkleasmium sp. dzf12 by oligosaccharides from its host plant dioscorea zingiberensis.three crude oligosaccharides were respectively prepared by acid hydrolysis of three polysaccharides, which were water-extracted polysaccharide (wep), sodium hydroxide-extracted polysaccharide (sep) and acid-extracted polysaccharide (aep) from the rhizomes of dioscorea zingiberensis. among the three oligosaccharides, the crude oligosaccharide prepared by acid hydrolysis of wep was found to be the most efficient elicitor to enhance the production of palmarumycins c(12) and c(13) in liquid culture ...201222450685
effects of polysaccharide elicitors from endophytic fusarium oxysporium dzf17 on growth and diosgenin production in cell suspension culture of dioscorea zingiberensis.three polysaccharides, namely exopolysaccharide (eps), water-extracted mycelial polysaccharide (wps) and sodium hydroxide-extracted mycelial polysaccharide (sps), were prepared from the endophytic fungus fusarium oxysporium dzf17 isolated from the rhizomes of dioscorea zingiberensis. the effects of the time of addition and polysaccharide concentration on the growth and diosgenin accumulation in cell suspension culture of d. zingiberensis were studied. among them, wps was found to be the most eff ...201122031064
deltonin isolated from dioscorea zingiberensis inhibits cancer cell growth through inducing mitochondrial apoptosis and suppressing akt and mitogen activated protein kinase signals.deltonin is an active component purified from dioscorea zingiberensis wright (dzw), and has shown anticancer effects. however, its mechanism of action remains elusive. in the present study, we investigated the effect of deltonin on a panel of cancer cell lines and analyzed its mechanism in c26 cells, a murine colon carcinoma cell. our results showed that deltonin markedly inhibited the growth of all examined cancer cell lines. deltonin induced dose- and time-dependent apoptosis in c26 cells. the ...201121804211
[electricity generation and contaminants degradation performances of a microbial fuel cell fed with dioscorea zingiberensis wastewater].the electricity generation performance of a microbial fuel cell (mfc) utilizing dioscorea zingiberensis wastewater was studied with an h-shape reactor. indexes including ph, conductivity, oxidation peak potential and chemical oxygen demand (cod) of the anolyte were monitored to investigate the contaminants degradation performance of the mfc during the electricity generation process, besides, contaminant ingredients in anodic influent and effluent were analyzed by gc-ms and ir spectra as well. th ...201121404685
treatment of wastewater from dioscorea zingiberensis tubers used for producing steroid hormones in a microbial fuel cell.a two-chamber microbial fuel cell (mfc) was used to treat dioscorea zingiberensis processing wastewater and generate electricity. the contaminant degradation process was systematically investigated with the help of uv-vis, ftir spectra and gc-ms. the results showed that the cod removal efficiency of the mfc reached 93.5% and the maximum power density achieved 175 mw/m(2). in the anodic chamber, low molecule weight acid, sugars and cellulose in d. zingiberensis processing wastewater were complete ...201121129952
production of diosgenin from yellow ginger (dioscorea zingiberensis c. h. wright) saponins by commercial cellulase.a commercial cellulase was first assessed to be effective in hydrolyzing glycosyl at the c-3 and c-26 positions in steroidal saponins from yellow ginger (dioscorea zingiberensis c. h. wright) to diosgenin, a very important chemical in the pharmaceutical industry. the effect of different parameters on enzyme hydrolysis was further investigated by systematically varying them. the highest yield was achieved when the hydrolysis ran at 55°c and ph 5.0 with an enzyme to substrate ratio of 15 × 10(3) u ...201024026920
characterization of steroidal saponins in crude extracts from dioscorea zingiberensis c. h. wright by ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry.steroidal saponins are the major bioactive constituents of dioscorea zingiberensis c. h. wright (d. zingiberensis). in this work, ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry (uplc/q-tof-ms/ms) was applied to the separation and characterization of steroidal saponins in crude extracts from d. zingiberensis. the results showed that fragment ions from glycosidic and cross-ring cleavages gave a wealth of structural information rel ...201020561762
in vitro tetraploid induction and generation of tetraploids from mixoploids in dioscorea zingiberensis.this article describes an efficient colchicine-mediated technique for the in vitro induction of tetraploids in dioscorea zingiberensis and its confirmation by flow cytometry. buds immersed in 0.2% colchicine solution for 36 hours prior to culture induced as high as 35.6% tetraploid plants. colchicine-induced tetraploids remained stable after six months in soil. leaf characteristics of diploids and tetraploids in d. zingiberensis were compared. it was determined that the leaf sizes of glasshouse- ...201020548936
[research of steroidal saponins from the fresh rhizomes of dioscorea zingiberensis].to study the steroidal saponins from the fresh rhizomes of dioscorea zingiberensis and search for new bioactive compounds.201020518308
[performance of a single chamber microbial fuel cell utilizing dioscorea zingiberensis c. h. wright wastewater].the possibility of electricity generation in a single chamber microbial fuel cell fed with dioscorea zingiberensis c. h. wright wastewater was demonstrated, and the effects of cod and so4(2-) concentration on mfc performance were investigated. under the same conductivity and cod concentration, the power density generated from wastewater equaled to 80.3% of that from glucose. at low cod concentration, the electricity generation increased with increasing cod loading rates, and the maximum power de ...200919968137
spirobisnaphthalenes from the endophytic fungus dzf12 of dioscorea zingiberensis and their antimicrobial activities.five spirobisnaphthalenes, namely palmarumycin cp17 (1), diepoxin kappa (2), diepoxin eta (3), diepoxin xi (4), and diepoxin gamma (5), were isolated from the endophytic fungus dzf12 associated with the medicinal plant dioscorea zingiberensis c. h. wright. their structures were identified by physicochemical and spectrometric analysis. among these spirobisnaphthalenes, 2 was found to have antibacterial activity, and the mixture of 3 and 4 was detected to have both antibacterial and antifungal act ...200919967975
process optimization for the production of diosgenin with trichoderma reesei.based on the response surface methodology, an effective microbial system for diosgenin production from enzymatic pretreated dioscorea zingiberensis tubers with trichoderma reesei was studied. the fermentation medium was optimized with central composite design (3(5)) depended on plackett-burmann design which identified significant impacts of peptone, k(2)hpo(4) and tween 80 on diosgenin yield. the effects of different fermentation conditions on diosgenin production were also studied. four paramet ...201019916029
production of diosgenin from dioscorea zingiberensis tubers through enzymatic saccharification and microbial transformation.in order to develop a clean and effective approach for producing the valuable drug diosgenin from dioscorea zingiberensis tubers, two successive processes, enzymatic saccharification and microbial transformation, were used. with enzymatic saccharification, 98.0% of starch was excluded from the raw herb, releasing saponins from the network structure of starch. subsequently, the treated tubers were fermented with trichoderma reesei under optimal conditions for 156 h. during microbial transformatio ...201019730849
tetraploidization of diploid dioscorea results in activation of the antioxidant defense system and increased heat tolerance.polyploidy is reported to show increased tolerance to environmental stress. in this work, tetraploid plants of dioscorea zingiberensis were obtained by colchicine treatment of shoots propagated in vitro. the highest tetraploid induction rate was achieved by treatment with 0.15% colchicine for 24h. diploid and tetraploid plants were exposed to normal (28 degrees c) and high temperature (42 degrees c) for 5d during which physiological indices were measured. compared with diploid plants, relative e ...201019692145
[isolation and structure determination of steroidal saponin from dioscorea zingiberensis].to study the chemical constituents of dioscorea zingiberensis wright, the etoh extract of fresh rhizomes of d. zingiberensis was concentrated and partitioned further to produce petroleum ether-, ethylacetate-, n-butanol- and water-soluble fractions. the water-soluble fraction was subjected to column chromatography on macro resin ab-8, and the final products were obtained by repeated reversed-phase ods and mci gel chp 20p column chromatography. structures of compounds were elucidated by 1h nmr, 1 ...200919350822
pilot treatment of wastewater from dioscorea zingiberensis c.h. wright production by anaerobic digestion combined with a biological aerated filter.an efficient combined system based on modified two-phase anaerobic digestion (mtpad) combined with a biological aerated filter (baf) is proposed to treat wastewater generated in the production of dioscorea zingiberensis c.h. wright (dzw). the pilot-scale experiments showed that both organics and sulfates at high concentrations could be removed satisfactorily due to the advantages of the mtpad in eliminating the negative effects of sulfide inhibition to methanogens. simultaneous nitrification and ...200919268578
[isolation and structure determination of steroidal saponin from dioscorea zingiberensis].to study the chemical constituents of dioscorea zingiberensis wright, the etoh extract of fresh rhizomes of d. zingiberensis was concentrated and partitioned further to produce petroleum ether-, ethylacetate-, n-butanol- and water-soluble fractions. the water-soluble fraction was subjected to column chromatography on macro resin ab-8, and the final products were obtained by repeated reversed-phase ods and mci gel chp 20p column chromatography. structures of compounds were elucidated by means of ...200718300473
[exploring genetic diversity in dioscorea zingiberensis by amplified fragment length polymorphism molecular markers].amplified fragment length polymorphism (aflp) was used to study 30 individuals from 5 wild populations of dioscorea zingiberensis for the first time. a total of 14 698 bands were detected with 9 pairs of aflp primers and 12 686 of them were polymorphic. on the average each primer combination could be used to detect 230 polymorphic bands and account for 85.92% of total genetic diversity at species level. shannonos index of diversity (i) was 0.3656-/+0.1721, and nei's gene diversity (h) was 0.2322 ...200717675758
[study on steroidal compounds from dioscorea zingiberensis].to study the chemical constituents of dioscorea zingiberensis.200617228657
[study on fertillizer application techniques of annual dioscoreae zingiberensis].the experiment n,p and k fertilizer on dioscorea zingiberensis were studied by setting fied parameter tests with rotational design. the selecton value of three agronomic factors were put forward by establishing regression mathematics models in the production and net value.200616913481
[effect of n, p, k on rhizome yield and diosgenin content of dioscorea zingiberensis].a field experiment was conducted to investigate the different effects of various applying ratios of nitrogen (n), potassium (k) and phosphate (p) on diosgenin content and rhizoma yield of dioscorea zingiberensis.200415807235
[studies on plantlet regeneration from the mature leaves of dioscorea zingiberensis].to establish a protocol of rapid clonal propagation of dioscorea zingiberensis using mature leaves as explants.200415719676
[studies on the dynamic trends of diosgenin content in vegetative organs of dioscorea zingiberensis].diosgenin was determined by hplc to study dynamic trend of diosgenin content in vegetative organs especially the rhizome, and the differences of diosgenin content between strains and between male and female rhizomes of dioscorea zingiberensis c. h. wright. the results are as follows: diosgenin content of biennial seedling rhizome is higher than that of annual seedling rhizome; diosgenin content of biennial rhizome derived from vegetative propagation is higher than that of annual rhizome derived ...200415323424
[advances in the study on dioscorea zingiberensis c.h. wright]. 200314631920
a new steroidal saponin from dioscorea zingiberensis wright.two compounds were obtained from the rhizome of dioscorea zingiberensis wright. one of them was elucidated as dioscin, the other one was a new compound and characterized as spirost-5-en-3-yl beta-d-glucopyranosyl (1 --> 3)-beta-d-glucopyranosyl (1 --> 4)-[alpha-l-rhamnopyranosyl (1 --> 2)]-beta-d-glucopyranoside, structure was determined on the basis 2d nmr spectroscopic technique and the result of acid hydrolysis.200312822909
[studies on the molluscicidal effect of dioscorea zingiberensis against oncomelania hupensis]. 200112572009
influence of different light intensities on the content of diosgenin, lipids, carotenoids and fatty acids in leaves of dioscorea zingiberensis.cultivation of the climbing plant dioscorea zingiberensis at a light intensity of 100 microe. m(-2) sec(-1) yields three different phenotypes. most of the plants grow as green phenotype (dzw). two further forms differ in their leaf shape and leaf color. whereas one type exhibits a more pointed leaf shape in the upper part of the plant with leaves appearing yellow-green with white stripes or hatchings (dzy), the other type shows a more round leaf shape with an intensive yellow-green color (dzt). ...201311926525
[the change of diosgenin content in cultivated dioscorea zingiberensis and d. panthaica from guizhou province].in this paper, the change of diosgenin content in cultivated dioscorea zingiberensis c.h. wright and d. panthaica prain et burk from guizhou province was studied. the results showed that the content of diosgenin attained 2.90% and 1.86% respectively. the change of diosgenin content in the phenophases is in keeping with the domestic reports.200111715189
[studies on immobilization of suspension cells of peltate yam (dioscorea zingiberensis c.h. wright)].the suspension cells of d. zingiberensis were immobilized with 3% sodium alginate, and then cultured in ms+2, 4-d1.0 + 6-ba 0.1 at 25 degrees c for a long period of time. the culture fluid free from cells was extracted and analyzed by tlc. the result showed that the immobilized cells could secrete the main component of d. zingiberensis--diosgenin, but not consecutively.19947811362
[research on correlations between climatic factors and diosgenin content in dioscorea zingiberensis wright].dioscorea zingiberensis wright is native to china. in this paper correlations between seven climatic factors (mean annual temperature, mean annual relative humidity, mean annual 5 cm soil temperature, annual precipitation. annual sunshine hours, mean annual sunshine percentage and annual greater than or equal to 10 degrees c accumulative temperature) and diosgenin content has been reported. it is annual precipitation and mean annual 5 cm soil temperature that are real principal factors affecting ...19892618721
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