| resolving ambiguities in genetic typing of human enterovirus species c clinical isolates and identification of enterovirus 96, 99 and 102. | molecular methods, based on sequencing the region encoding the vp1 major capsid protein, have recently become the gold standard for enterovirus typing. in the most commonly used scheme, sequences more than 75% identical (>85% amino acid identity) in complete or partial vp1 sequence are considered to represent the same type. however, as sequence data have accumulated, it has become clear that the '75%/85% rule' may not be universally applicable. to address this issue, we have determined nucleotid ... | 2009 | 19264596 |
| the complete genome sequences for a novel enterovirus type, enterovirus 96, reflect multiple recombinations. | enterovirus 96 (ev-96) is a recently described genotype in the species human enterovirus c. so far, only partial genome sequences of this enterovirus type have been available. in this study, we report complete genome sequences for two ev-96 strains isolated from healthy children during enterovirus surveillance in finland. sequence analysis revealed substantial nucleotide divergence between ev-96 strains and suggested several recombination events between ev-96 and other hev-c types. | 2009 | 19526351 |
| development of a reverse transcription-loop-mediated isothermal amplification (rt-lamp) system for a highly sensitive detection of enterovirus in the stool samples of acute flaccid paralysis cases. | in the global eradication program for poliomyelitis, the laboratory diagnosis plays a critical role by isolating poliovirus (pv) from the stool samples of acute flaccid paralysis (afp) cases. in this study, we developed a reverse transcription-loop-mediated isothermal amplification (rt-lamp) system for a rapid and highly sensitive detection of enterovirus including pv to identify stool samples positive for enterovirus including pv. | 2009 | 20015403 |
| the complete genome analysis of two enterovirus 96 strains isolated in china in 2005 and 2009. | enterovirus 96 (ev96) is a new member of species human enterovirus c (hev-c). in this report, genomic characterization of two ev96 strains isolated from acute flaccid paralysis surveillance in shandong province of china in 2005 and 2009 is described. the two strains, designated 05517 and 09228c1, had 82.7% genomic similarity with each other and 75.1-84.2% with other three strains available from genbank in complete genome sequences. in vp1 coding region, they had 77.6-86.6% nucleotide similarity ... | 2011 | 21318238 |