Publications

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identification of euryhelmis costaricensis metacercariae in the skin of tohoku hynobiid salamanders (hynobius lichenatus), northeastern honshu, japan.since 2000, cutaneous nodular lesions were observed in tohoku hynobiid salamanders (hynobius lichenatus) in the northern abukuma mountains, northeastern honshu, japan. the distribution of diseased salamanders has expanded, along with the increasing severity of the disease. samples (foreleg, hindleg, and tail) from five diseased salamanders caught in the spring of 2009 contained 11 to 383 metacercarial cysts (0.43-0.48 mm in diameter) per individual. experimental inoculations of the metacercariae ...201020688689
locomotion pattern and trunk musculoskeletal architecture among urodela.we comparatively examined the trunk musculature and prezygapophyseal angle of mid-trunk vertebra in eight urodele species with different locomotive modes (aquatic siren intermedia, amphiuma tridactylum, necturus maculosus and andrias japonicus; semi-aquatic cynops pyrrhogaster, cynops ensicauda; and terrestrial hynobius nigrescens, hynobius lichenatus and ambystoma tigrinum). we found that the more terrestrial species were characterized by larger dorsal and abdominal muscle weight ratios compare ...201525914411
observations on the migration and differentiation of neural crest cells in somite extirpated salamander larvae.in order to research the influences of the somites or their derivatives on the migration and differentiation of neural crest cells, unilateral extirpation of somites was performed in the larvae of the salamander (hynobius lichenatus boulenger). two types of neural crest cells appeared in the somite-free environment. they could already be distinguished from each other shortly after beginning of migration. one type of the cells was distributed in the expanded space between the epidermis and the ne ...19863800594
papilloma in hynobius lichenatus boulenger 1883 (amphibia, urodela). 19883239163
mitochondrial cytochrome b phylogeny and historical biogeography of the tohoku salamander, hynobius lichenatus (amphibia, caudata).the tohoku salamander, hynobius lichenatus boulenger, 1883, is a lentic breeding species widespread throughout montane regions of northeastern japan. to explore intraspecific genetic variation and infer evolutionary history of h. lichenatus, we performed mitochondrial dna analysis (complete 1141 bp sequences of the mitochondrial cytochrome b gene) using 215 adult and larval individuals collected from 75 localities, encompassing known distributional range of the species. hynobius lichenatus prove ...201323480375
effects of chronic γ-irradiation on growth and survival of the tohoku hynobiid salamander, hynobius lichenatus.the tohoku hynobiid salamanders, hynobius lichenatus, were chronically irradiated with γ-rays from embryonic to juvenile stages for 450 days. at 490 μgy h(-1) or lower dose rates, growth and survival were not significantly affected by irradiation, and any morphological aberrations and histological damages were not observed. at 4600 μgy h(-1), growth was severely inhibited, and all the individuals died mostly at the juvenile stage. chronic ld50 was 42 gy as a total dose. in the liver, the number ...201424797100
dose rate estimation of the tohoku hynobiid salamander, hynobius lichenatus, in fukushima.the radiological risks to the tohoku hynobiid salamanders (class amphibia), hynobius lichenatus due to the fukushima dai-ichi nuclear power plant accident were assessed in fukushima prefecture, including evacuation areas. aquatic egg clutches (n = 1 for each sampling date and site; n = 4 in total), overwintering larvae (n = 1-5 for each sampling date and site; n = 17 in total), and terrestrial juveniles or adults (n = 1 or 3 for each sampling date and site; n = 12 in total) of h. lichenatus were ...201525765872
effects of chronic γ-irradiation on growth and sexual maturation of the tohoku hynobiid salamander, hynobius lichenatus.there are still considerable gaps in knowledge regarding the biological effects of chronic ionising radiation exposure in amphibians. to fill these gaps, tohoku hynobiid salamanders, hynobius lichenatus (amphibia, caudata), were chronically irradiated with 137cs γ-rays from embryonic to adult stages over 1954 days, and the effects on their growth and sexual maturation were examined under laboratory conditions. irradiation at a dose rate of 33 μgy h-1 had some stimulatory effects on growth (body ...201930423483
cloning of hynobius lichenatus (tohoku hynobiid salamander) p53 and analysis of its expression in response to radiation.caudata species such as salamanders are easily affected by environmental changes, which can drastically reduce their population. the effects of acute x-rays and chronic γ-irradiation on hynobius lichenatus, the japanese tohoku hynobiid salamander, are known. however, the expression of radiation-inducible genes, such as the dna-damage checkpoint response gene p53, has not been analyzed in h. lichenatus. this has not occurred because there is no established method for mrna quantification in h. lic ...202032434469
sperm motility-initiating activity in the egg jelly of the externally-fertilizing urodele amphibian, hynobius lichenatus.low osmolality initiates sperm motility during the external fertilization of aquatic anuran amphibians. it is thought that this process occurs also in urodeles, but this has not been fully examined in these species. we report here that fertilization was achieved in the externally fertilizing hynobiid, hynobius lichenatus, by direct insemination onto the egg jelly surface without initial exposure of the sperm to a hypoosmotic solution. to identify the factors in addition to low osmolality that in ...201021039127
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