| diversity of nodule-endophytic agrobacteria-like strains associated with different grain legumes in tunisia. | this study represents the first report describing the genetic diversity of nodule-endophytic agrobacteria isolated from diverse legumes and their phylogenetic relationships with the valid species of agrobacteria, as well as the non-recognized genomospecies of the former agrobacterium tumefaciens (rhizobium radiobacter). the genetic diversity of a collection of 18 non-nodulating agrobacteria-like strains, previously isolated from root nodules of vicia faba, cicer arietinum and phaseolus vulgaris ... | 2011 | 21621936 |
| maldi-tof mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family rhizobiaceae. | family rhizobiaceae includes fast growing bacteria currently arranged into three genera, rhizobium, ensifer and shinella, that contain pathogenic, symbiotic and saprophytic species. the identification of these species is not possible on the basis of physiological or biochemical traits and should be based on sequencing of several genes. therefore alternative methods are necessary for rapid and reliable identification of members from family rhizobiaceae. in this work we evaluated the suitability o ... | 2011 | 21655291 |
| biodegradation of nicotine by a novel strain shinella sp. hzn1 isolated from activated sludge. | the nicotine-degrading bacterium hzn1 was isolated from activated sludge and identified as shinella sp. based on its physiological characteristics and analysis of 16s rdna gene. strain hzn1 is capable of using nicotine as the sole carbon source in the mineral salts medium. the optimum temperature and ph for strain hzn1 growth and nicotine degradation were 30°c and 7.0, respectively. it could degrade approximately 100 % of 0.5 g l(-1) of nicotine within 9 h. three intermediate metabolites were pr ... | 2011 | 21864140 |
| [screening and identification of two heterotrophic nitrifying bacteria and characterization of their capacity for nitrogen removal]. | two heterotrophic nitrifying bacteria, p2 and p9 isolated from piggery wastewater, were studied for their capacity of nitrification and nitrogen removal. | 2011 | 22233060 |
| shinella fusca sp. nov., isolated from domestic waste compost. | a bacterium, designated strain dc-196(t), isolated from kitchen refuse compost was analysed by using a polyphasic approach. strain dc-196(t) was characterized as a gram-negative short rod that was catalase- and oxidase-positive, and able to grow at 10-40 degrees c, ph 6-9 and in nacl concentrations as high as 3 %. chemotaxonomically, c(18 : 1) was observed to be the predominant cellular fatty acid and ubiquinone 10 (q10) was the predominant respiratory quinone. the g+c content of the genomic dna ... | 2010 | 19648331 |
| shinella daejeonensis sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant. | a gram-negative, motile, rod-shaped, aerobic bacterial strain, designated mj02t, was isolated from sludge of a leachate treatment plant in daejeon (south korea) and was characterized to determine its taxonomic position by using a polyphasic approach. comparative 16s rrna gene sequence analysis showed that strain mj02t belongs to the family rhizobiaceae, class alphaproteobacteria, and is related most closely to shinella yambaruensis ms4t (97.6 % sequence similarity) and shinella fusca dc-196t (97 ... | 2010 | 20870886 |
| [study on the function of plasmid in pyridine-degrading bacteria]. | in order to identify the characteristics of the plasmids of degrading bacterial strains and the relationship between the plasmids' function and biodegradation, plasmids were isolated from two bacterial strains (paracoccus sp. bw001 and shinella zoogloeoides bc026) and pulse-field gel electrophoresis was used to identify the distribution of plasmids and their molecular size. two large plasmids with 190-245 kb and one small plasmid with 4.5-5.0 kb were found in the bw001, and at least 3 large plas ... | 2010 | 20825045 |
| aerobic degradation of pyridine by a new bacterial strain, shinella zoogloeoides bc026. | a new bacterial strain, shinella zoogloeoides bc026, which utilizes pyridine as its sole carbon, nitrogen and energy source, was isolated from the activated sludge of a coking wastewater treatment plant. the bc026 strain completely degraded up to 1,806 mg/l of pyridine in 45.5 h. the optimum degradation conditions were ph 8.0 and temperature 30-35 degrees c. according to product monitoring and genetic analysis, the pyridine ring was cleaved between c(2) and n, resulting in 58% of pyridine-n bein ... | 2009 | 19669816 |
| shinella yambaruensis sp. nov., a 3-methyl-sulfolane-assimilating bacterium isolated from soil. | a bacterial strain, designated ms4(t), was isolated from soil in the ryukyu archipelago, japan. the bacterium grew with 3-methyl sulfolane as sole sulfur source. phylogenetic analysis based on 16s rrna gene sequences suggested that strain ms4(t) belonged to the genus shinella; it was closely related to the type strains of shinella granuli and shinella zoogloeoides (16s rrna gene sequence similarities of 98.2 and 96.7 %, respectively). strain ms4(t) was a gram-negative, non-motile, rod-shaped, ae ... | 2009 | 19244436 |
| [biodegradation of pyridine by shinella zoogloeoides bc026]. | a bacterial strain bc026 capable of utilizing pyridine as its sole source of carbon and nitrogen was isolated from the activated sludge in a coking wastewater treatment plant. the bacterium featured flocculability and antibiotic resistance to kanamycin, ampicillin and spectinomycine. it could grow well in ashby nitrogen free culture medium. the strain was identified as shinella zoogloeoides according to the results of 16s rrna sequence analysis and biolog microbial identification system. the exp ... | 2008 | 19143398 |
| diverse bacteria isolated from root nodules of wild vicia species grown in temperate region of china. | in the present study, a total of 154 bacterial strains isolated from nodules of eighteen vicia species mainly grown in the temperate chinese provinces were characterized by ardra, its pcr-rflp, box-pcr, sequencing of 16s rdna, nodc, nifh, atpd and glnii, and nodulation tests. the results demonstrated that most of the r. leguminosarum strains were effective microsymbionts of the wild vicia species, while genomic species related to rhizobium gallicum, mesorhizobium huakuii, ensifer meliloti and br ... | 2008 | 18704366 |
| zoogloea oryzae sp. nov., a nitrogen-fixing bacterium isolated from rice paddy soil, and reclassification of the strain atcc 19623 as crabtreella saccharophila gen. nov., sp. nov. | two strains of free-living diazotrophs isolated from soil from a rice paddy field were characterized by using a polyphasic approach. the novel strains, a-7t and a-4, were found to be very closely related, with 99.9% 16s rrna gene sequence similarity and a dna-dna hybridization value of 89.5%, suggesting that they represent a single species. 16s rrna gene sequence analyses indicated that the two strains fell within the zoogloea lineage, with less than 96.7 % sequence similarity to other zoogloea ... | 2006 | 16514038 |
| shinella kummerowiae sp. nov., a symbiotic bacterium isolated from root nodules of the herbal legume kummerowia stipulacea. | bacterial strain ccbau 25048(t) was isolated from root nodules of kummerowia stipulacea grown in shandong province of china. cells of the strain were gram-negative, strictly aerobic, non-spore-forming, motile short rods. phylogeny of 16s rrna gene sequences revealed that the strain belonged to the genus shinella, a member of family rhizobiaceae. its closest phylogenetic relatives were shinella granuli ch06(t) and shinella zoogloeoides iam 12669(t), respectively showing 98.3 and 98.9 % 16s rrna g ... | 2008 | 18523187 |
| hrt and nutrients affect bacterial communities grown on recirculation aquaculture system effluents. | in a recirculation aquaculture system the drumfilter effluent can be used as substrate for heterotrophic bacterial production, which can be recycled as feed. because the bacteria might contain pathogens, which could reduce its suitability as feed, it is important to characterize these communities. bacteria were produced in growth reactors under different conditions: 7 h hydraulic retention time (hrt) vs. 2 h, sodium acetate vs. molasses, and ammonia vs. nitrate. the community of the drumfilter e ... | 2007 | 17371322 |
| community structure analysis of reverse osmosis membrane biofilms and the significance of rhizobiales bacteria in biofouling. | the biofilm community structure of a biofouled reverse osmosis (ro) membrane was examined using a polyphasic approach, and the dominant phylotypes retrieved were related to the order rhizobiales, a group of bacteria that is hitherto not implicated in membrane biofouling. a comparison with two other membrane biofilms using t-rflp fingerprinting also revealed the dominance of rhizobiales organisms. when pure culture ro biofilm isolates were cultivated aerobically in biolog microplates, most rhizob ... | 2007 | 17695921 |
| shinella granuli gen. nov., sp. nov., and proposal of the reclassification of zoogloea ramigera atcc 19623 as shinella zoogloeoides sp. nov. | the taxonomic position of a novel bacterial strain, ch06t, isolated from an upflow anaerobic sludge blanket reactor was determined. strain ch06t was gram-negative, aerobic, motile and oxidase- and catalase-positive. a comparative 16s rrna gene sequence analysis showed a clear affiliation of strain ch06t to the alphaproteobacteria and it was most closely related to zoogloea ramigera atcc 19623 and mycoplana dimorpha iam 13154t (97.9 and 96.3% sequence similarity, respectively). the major respirat ... | 2006 | 16449455 |