development of a multiplex polymerase chain reaction for simultaneous detection of wheat viruses and a phytoplasma in china. | barley yellow dwarf viruses (bydvs, mainly consisting of three strains, gav, gpv and pav, in china), barley stripe mosaic virus (bsmv), wheat yellow mosaic virus (wymv), wheat dwarf virus (wdv) and wheat blue dwarf phytoplasma (wbd) constitute a group of major wheat pathogens that have caused huge yield losses but are scarcely distinguished by their phenotype alone. for the simultaneous detection and discrimination of these seven pathogens in wheat, a multiplex polymerase chain reaction (m-pcr) ... | 2012 | 22456911 |
comparative genome analysis of wheat blue dwarf phytoplasma, an obligate pathogen that causes wheat blue dwarf disease in china. | wheat blue dwarf (wbd) disease is an important disease that has caused heavy losses in wheat production in northwestern china. this disease is caused by wbd phytoplasma, which is transmitted by psammotettix striatus. until now, no genome information about wbd phytoplasma has been published, seriously restricting research on this obligate pathogen. in this paper, we report a new sequencing and assembling strategy for phytoplasma genome projects. this strategy involves differential centrifugation, ... | 2014 | 24798075 |