| identification of forensically important sarcophagid flies (diptera: sarcophagidae) in china, based on coi and 16s rdna gene sequences. | insects attracted to cadavers may provide important indications of the postmortem interval (pmi). however, use of the flesh flies (diptera: sarcophagidae) for pmi estimation is limited as the species are often not morphologically distinct, especially as immatures. in this study, 23 forensically important flesh flies were collected from 13 locations in 10 chinese provinces. then, a 278-bp segment of the cytochrome oxidase subunits one (coi) gene and a 289-bp segment of the 16s rdna gene of all sp ... | 2011 | 21854377 |
| forensically important flesh fly species in thailand: morphology and developmental rate. | forensically important flesh fly species in thailand have been investigated for their larval morphology and developmental rate to increase the forensic entomology database in thailand and nearby countries. sarcophaga (liosarcophaga) dux thomson, sarcophaga (liopygia) ruficornis (fabricius), and sarcophaga (boettcherisca) peregrina (robineau-desvoidy) are the species of greatest forensic importance. the third instars of these species are similar in morphological appearance, making it difficult or ... | 2010 | 20145954 |
| dna-based identification of forensically important australian sarcophagidae (diptera). | the utility of the forensically important sarcophagidae (diptera) for time since death estimates has been severely limited, as morphological identification is difficult and thermobiological histories are inadequately documented. a molecular identification method involving the sequencing of a 658-bp 'barcode' fragment of the mitochondrial cytochrome oxidase subunit i (coi) gene from 85 specimens, representing 16 australian species from varying populations, was evaluated. nucleotide sequence diver ... | 2011 | 19997851 |
| ommatidia of blow fly, house fly, and flesh fly: implication of their vision efficiency. | this work aims to elucidate the number of ommatidia or facets (the outwardly visible units of each ommatidium) for compound eyes in blow flies [chrysomya megacephala (f.), chrysomya rufifacies (macquart), chrysomya nigripes (aubertin), lucilia cuprina (wiedemann)], house flies (musca domestica l.), and flesh flies (liosarcophaga dux thomson) by manual counts of the corneal spreads. the head of the fly in each species was soaked in 20% potassium hydroxide solution at room temperature for 7 days, ... | 2008 | 18343951 |
| ultrastructure of adhesive device in fly in families calliphoridae, muscidae and sarcophagidae, and their implication as mechanical carriers of pathogens. | the ultrastructure of adhesive device or the pulvilli, pad-like structure between the tarsal claws of the legs, is presented in the blowfly (calliphoridae), housefly and relatives (muscidae), and flesh fly (sarcophagidae) through scanning electron microscopy. the blowfly species were chrysomya chani, chrysomya nigripes, chrysomya pinguis, and chrysomya villeneuvi; while those of the housefly and relatives were musca domestica and hydrotaea chalcogaster, respectively. fresh fly species included b ... | 2006 | 16416126 |
| surface ultrastructure of the puparia of the blow fly, lucilia cuprina (diptera: calliphoridae), and flesh fly, liosarcophaga dux (diptera: sarcophagidae). | surface ultrastructure of the puparia of the blow fly, lucilia cuprina, and flesh fly, liosarcophaga dux, are presented utilizing scanning electron microscopy (sem). emphasis was focused on characteristic features of the puparia that could be used for differentiation from other forensically important fly species. the puparium of l. cuprina typically measures 6.2+/-0.2 mm in length and 2.3+/-0.1 mm in width. each anterior spiracle contains a single row of 5-7 papillae. the intersegmental spines b ... | 2006 | 16416125 |
| male genitalia of flesh fly parasarcophaga (liosarcophaga) dux (diptera: sarcophagidae) revealed by scanning electron microscopy. | the male genitalia of flesh fly parasarcophaga (liosarcophaga) dux (diptera: sarcophagidae) was observed using scanning electron microscopy (sem). the phallus is a short, broad structure that is formed by a tubular base connected to a trumpet-shaped, anteroventrally expanded ventralia. the juxta projects forward from a broad base, which is connected to an upright long structure, the harpes. the juxta is apically bifurcated. the lateral styli bear three small spines curved inward. the pregonite a ... | 2007 | 17536374 |
| antennal sensilla of some forensically important flies in families calliphoridae, sarcophagidae and muscidae. | antennal sensilla of some forensically important fly species in the families calliphoridae (chrysomya megacephala, chrysomya rufifacies, chrysomya nigripes and lucilia cuprina), sarcophagidae (parasarcophaga dux) and muscidae (musca domestica) were studied using scanning electron microscopy. five types of sensilla were observed: trichoid, basiconic, coeloconic, styloconic and sensory pit. only trichoid sensilla are found on the scape of the antenna, while both trichoid and styloconic sensilla ar ... | 2004 | 15288645 |
| enzyme activity in the flesh fly parasarcophaga dux thomson influenced by dill compounds, myristicin and apiol. | the newly emerged adults of parasarcophaga dux were treated topically with various doses of myristicin and apiol isolated from roots of dill plant, anethum graveolus. the compounds toxicity and the dehydrogenase activities of the treated stage and its subsequent developmental stages were studied. the results indicated that apiol more toxic than myristicin. the spectrophotometric evaluation exhibited changes in dehydrogenase activities after treatments. compounds increased the activities of both ... | 2004 | 15125531 |
| toxicological efficacy of some indigenous dill compounds against the flesh fly, parasarcophaga dux thomson. | the effect of the lc50 of the three isolated compounds (apiol, myristicin and d-carvone) from dill, anethum graveolus on growth and reproduction of parasarcophaga dux showed that three compounds especially apiol caused significant reduction in the percentage of adults emergence and females fecundity. the temperature toxicity relation shape of the three compounds and five insect growth regulations (methoprene, hydroprene, teflubenzuron, chlorfluazuron and precocene i) alone or in combination agai ... | 2004 | 15125529 |
| prestomal teeth of some flies of medical importance. | comparison of prestomal teeth of adult chrysomya megacephala, chrysomya rufifacies, chrysomya nigripes, lucilia cuprina, parasarcophaga dux and musca domestica was accomplished by use of scanning electron microscopy. the prestomal teeth of c. megacephala, c. rufifacies, l. cuprina and p. dux are all similar in appearance in having various degrees of bifurcation at their tips. in contrast, the tips of the prestomal teeth in c. nigripes are very shallowly serrated, but are comparatively more deepl ... | 2003 | 14680932 |
| larval ultrastructure of parasarcophaga dux (thomson) (diptera: sarcophagidae). | ultrastructure of all larval instars of parasarcophaga dux (thomson), a common flesh fly species in thailand, is presented using scanning electron microscopy. special attention is given to the structure of anterior and posterior spiracles since these are important features used to differentiate between other sarcophagids. each anterior spiracle in second and third instars has a single row of papillae varying in number from 14 to 17. the posterior spiracular discs have incomplete peritremes, with ... | 2003 | 14680921 |
| identification of the forensically important sarcophagid flies boerttcherisca peregrina, parasarcophaga albiceps and parasarcophaga dux (diptera: sarcophagidae) based on coii gene in china. | accurate species identication is a crucial step in forensic entomology, as the insect collected on a corpse can provide useful information for estimation of postmortem interval (pmi). the utility of the forensically important sarcophagidae (diptera) for crime scene investigation has been severely restricted, as morphological identification is difficult, especially the identification of females and larvae. in this study, a method for using mitochondrial dna (mtdna) sequence data and phylogenetic ... | 2010 | 21399586 |
| sarcophaga (liosarcophaga) dux (diptera: sarcophagidae): a flesh fly species of medical importance. | although tropical climate of thailand is suitably endowed with biodiversity of insects, flies of medical importance is not well investigated. using information from literature search, fly survey approach and specialist's experience, we review database of sarcophaga (liosarcophaga) dux thomson (diptera: sarcophagidae), one of the priorities flesh fly species of medical importance in thailand. | 2014 | 25027360 |
| ultramorphology of eggshell of flesh fly liosarcophaga dux (diptera: sarcophagidae). | eggs of liosarcophaga dux (thomson) (diptera: sarcophagidae) are elongated and slightly bean-shaped, measuring approximately 1.5 mm in length. each is covered externally by an eggshell comprised of polygonal patterns. in this study, scanning and transmission electron microscopy were used to examine the eggshell of this species of flesh fly for the first time. no plastron region or median area was detected. ultrathin sectioning of the eggshell revealed multiple layers in the shell that could be o ... | 2005 | 15691014 |
| efficacy of baysir-8514 and precocene ii against the grey flesh fly parasarcophaga dux (thomson) (diptera: sarcophagidae). | five doses (1, 0.5, 0.25, 0.125 and 0.0625) of the chitin inhibitor baysir-8514, and anti-juvenoid precocene ii were topically applied to the third larval instar of the grey flesh fly parasarcophaga dux. toxicity of both compounds were recorded against different developmental stages at the different dose levels. the lowest dose (0.0625) of both compounds caused a low toxicity percent (0.1). on the other hand, the highest dose (1mg) increased the larval, pupal and adult malformations after larval ... | 1999 | 12561911 |
| identification of forensically important sarcophagidae (diptera) based on partial mitochondrial cytochrome oxidase i and ii genes. | entomological evidence is of great importance in forensic cases for postmortem interval calculation. the use of sarcophagidae (diptera) for postmortem interval estimation is limited because morphological determination is often hampered because of similar characteristics in the larval, pupal, and even adult stage. to make the species identification more accurate and reliable, dna-based identification is considered. in this study, we assessed the use of partial mitochondrial cytochrome oxidase i a ... | 2013 | 23629402 |
| applicability of partial characterization of cytochrome oxidase i in identification of forensically important flies (diptera) from china and egypt. | precise species identification of every insect sample collected from criminal scenes play an essential role in the accurate estimation of postmortem interval. the morphological similarity poses a great challenge for forensic entomologists. dna-based method can be used as a supplemental means of morphological method. in the present study, we demonstrate the applicability of the 304-bp cytochrome oxidase i gene fragment in molecular identification of forensically important diptera. we analyzed 75 ... | 2013 | 23661266 |