| pseudomonas otitidis sp. nov., isolated from patients with otic infections. | a novel pseudomonas species, for which the name pseudomonas otitidis sp. nov. is proposed, was identified from clinical specimens of infected human ears. forty-one pseudomonads (34 from patients with acute otitis externa, six from patients with acute otitis media with otorrhoea and one from a patient with chronic suppurative otitis media) were recovered that did not match any known species. on the basis of genetic analyses and biochemical characterization, these isolates were shown to belong to ... | 2006 | 16585681 |
| rapid and accurate detection of pseudomonas aeruginosa by real-time polymerase chain reaction with melting curve analysis targeting gyrb gene. | laboratory detection of pseudomonas spp., particularly pseudomonas aeruginosa, is an important assay in the nosocomial control. the study was designed firstly to establish a new assay-applied lightcycler polymerase chain reaction (pcr) technology with melting curve analysis (mca). a total of 224 gram-negative isolates were used to verify the assay system. the pcr with mca method using the p. aeruginosa-specific gyrase b gene primers was rapid and accurate; the total run is approximately 3 h, and ... | 2007 | 17368797 |
| isolation and characterization of pseudomonas otitidis wl-13 and its capacity to decolorize triphenylmethane dyes. | pseudomonas otitidis wl-13, which has a high capacity to decolorize triphenylmethane dyes, was isolated from activated sludge obtained from a wastewater treatment plant of a dyeing industry. this strain exhibited a remarkable color-removal capability when tested against several triphenylmethane dyes under both shaking and static conditions at high concentrations of dyes. more than 95% of malachite green and brilliant green was removed within 12 h at 500 micromol/l dye concentration under shaking ... | 2009 | 19862963 |
| metallo-beta-lactamase production by pseudomonas otitidis: a species-related trait. | susceptibility to several β-lactams and β-lactamase production was investigated in a collection of 20 strains of pseudomonas otitidis, a new pseudomonas species that has been recently recognized in association with otic infections in humans. all strains appeared to be susceptible to piperacillin, cefotaxime, ceftazidime, and aztreonam, while resistance or decreased susceptibility to carbapenems was occasionally observed. all strains were found to express metallo-β-lactamase (mbl) activity and to ... | 2010 | 21060106 |
| pom-1 metallo-β-lactamase-producing pseudomonas otitidis isolate from a patient with chronic otitis media. | | 2011 | 22209511 |
| biocontrol of phytophthora blight and anthracnose in pepper by sequentially selected antagonistic rhizobacteria against phytophthora capsici. | we previously developed a sequential screening procedure to select antagonistic bacterial strains against phytophthora capsici in pepper plants. in this study, we used a modified screening procedure to select effective biocontrol strains against p. capsici; we evaluated the effect of selected strains on phytophthora blight and anthracnose occurrence and fruit yield in pepper plants under field and plastic house conditions from 2007 to 2009. we selected four potential biocontrol strains (pseudomo ... | 2013 | 25288942 |
| biofilm-mediated enhanced crude oil degradation by newly isolated pseudomonas species. | the bioavailability of organic contaminants to the degrading bacteria is a major limitation to efficient bioremediation of sites contaminated with hydrophobic pollutants. such limitation of bioavailability can be overcome by steady-state biofilm-based reactor. the aim of this study was to examine the effect of such multicellular aggregation by naturally existing oil-degrading bacteria on crude oil degradation. microorganisms, capable of utilizing crude oil as sole carbon source, were isolated fr ... | 2013 | 25937972 |
| response of indigenously developed bacterial consortia in progressive degradation of polyvinyl chloride. | thermoplastic-based materials are recalcitrant in nature, which extensive use affect environmental health. here, we attempt to compare the response of indigenously produced bacterial consortium-i and consortium-ii in degrading polyvinyl chloride (pvc). these consortia were developed by using different combination of bacterial strains of pseudomonas otitidis, bacillus cereus, and acanthopleurobacter pedis from waste disposal sites of northern india after their identification via 16s rdna sequenci ... | 2016 | 26231814 |
| purification and characterization of glutaminase free asparaginase from pseudomonas otitidis: induce apoptosis in human leukemia molt-4 cells. | asparaginase is an important antineoplastic drug extensively used for the treatment of acute lymphoblastic leukemia, but the intrinsic glutaminase activity of this enzymatic drug is responsible for several life threatening side effects. this study describes the purification and characterization of glutaminase free asparaginase from pseudomonas otitidis. the purified enzyme exhibited molecular mass of approximately 205±3 kda on native-page and ̴34±1 kda on sds-page, revealing that the enzyme is h ... | 2016 | 26597158 |
| biochemical characterization of the pom-1 metallo-β-lactamase from pseudomonas otitidis. | the pom-1 metallo-β-lactamase is a subclass b3 resident enzyme produced by pseudomonas otitidis, a pathogen causing otic infections. the enzyme was overproduced in escherichia coli bl21(de3), purified by chromatography, and subjected to structural and functional analysis. the purified pom-1 is a tetrameric enzyme of broad substrate specificity with higher catalytic activities with penicillins and carbapenems than with cephalosporins. | 2015 | 25512428 |
| isolation of carbapenem-resistant pseudomonas spp. from food. | pseudomonas spp. are ubiquitous in nature. carbapenem resistance in environmental isolates of members of this genus is thought to be rare but the exact resistance rate is unknown. in this study, carbapenem-resistant pseudomonas spp. were isolated from chicken and pork samples and the mechanisms underlying the carbapenem resistance in these strains were investigated. a total of 16 carbapenem-resistant pseudomonas aeruginosa, pseudomonas putida and pseudomonas otitidis isolates were recovered from ... | 2015 | 27873658 |
| two non-otic cases of pom-1 metallo-β-lactamase-producing pseudomonas otitidis infection: necrotizing fasciitis and pan-peritonitis. | | 2016 | 27835844 |
| pseudomonas otitidis as a potential biocatalyst for polyhydroxyalkanoates (pha) synthesis using synthetic wastewater and acidogenic effluents. | polyhydroxyalkanoates (pha) production using pseudomonas otitidis, a newly isolated strain from pha producing bioreactor was investigated using synthetic acids (sa) and acidogenic effluents (ae) from biohydrogen reactor at different organic loading rates (olrs). p. otitidis showed ability to grow and accumulate pha, with simultaneous waste remediation. ae showed less pha production (54%, olr3), than sa (58%, olr2). pha composition showed co-polymer, poly-3(hydroxy butyrate-co-hydroxy valerate), ... | 2012 | 22940357 |
| lipase from marine strain using cooked sunflower oil waste: production optimization and application for hydrolysis and thermodynamic studies. | the marine strain pseudomonas otitidis was isolated to hydrolyze the cooked sunflower oil (cso) followed by the production of lipase. the optimum culture conditions for the maximum lipase production were determined using plackett-burman design and response surface methodology. the maximum lipase production, 1,980 u/ml was achieved at the optimum culture conditions. after purification, an 8.4-fold purity of lipase with specific activity of 5,647 u/mg protein and molecular mass of 39 kda was obtai ... | 2013 | 22833226 |