| rpob gene sequence-based characterization of emerging non-tuberculous mycobacteria with descriptions of mycobacterium bolletii sp. nov., mycobacterium phocaicum sp. nov. and mycobacterium aubagnense sp. nov. | over the past 10 years, 16s rrna gene sequencing has contributed to the establishment of more than 45 novel species of non-tuberculous mycobacteria and to the description of emerging mycobacterial infections. cumulative experience has indicated that this molecular tool underestimates the diversity of this group and does not distinguish between all recognized mycobacterial taxa. in order to improve the recognition of emerging rapidly growing mycobacteria (rgm), rpob gene sequencing has been devel ... | 2006 | 16403878 |
| multiphasic approach reveals genetic diversity of environmental and patient isolates of mycobacterium mucogenicum and mycobacterium phocaicum associated with an outbreak of bacteremias at a texas hospital. | between march and may 2006, a texas hospital identified five mycobacterium mucogenicum bloodstream infections among hospitalized oncology patients using fluorescence high-performance liquid chromatography analysis of mycolic acids. isolates from blood cultures were compared to 16 isolates from environmental sites or water associated with this ward. these isolates were further characterized by hsp65, 16s rrna, and rpob gene sequencing, hsp65 pcr restriction analysis, and molecular typing methods, ... | 2008 | 18310417 |
| mycobacterium phocaicum in therapy pool water. | we isolated mycobacterium phocaicum, an emerging non-tuberculous species rarely identified in the respiratory tract and blood of patients, from the therapy pool water. identification, confirmed by 16s rdna and rpob sequencing and phylogenetic analyses, disclosed a genotype 4. m. phocaicum should be added to the growing list of water-borne mycobacteria. | 2009 | 19201259 |
| utility of high-performance liquid chromatography analysis of mycolic acids and partial 16s rrna gene sequencing for routine identification of mycobacterium spp. in a national reference laboratory. | high-performance liquid chromatography analysis of mycolic acids and partial gene sequencing for the first 500-bp 5' end of the 16s rrna gene were used singularly and in combination to evaluate the final identification of species. examination of 200 cultures revealed 100 strains of slowly growing mycobacteria (sgm), 91 strains of rapidly growing mycobacteria (rgm), and 9 strains of other genera. sgm were discriminated in complexes with both methods for 56 strains, composed primarily of the mycob ... | 2010 | 20466195 |
| characterization of fluoroglycofen ethyl degradation by strain mycobacterium phocaicum mbwy-1. | a fluoroglycofen ethyl-degrading bacterium, mbwy-1, was isolated from the soil of an herbicide factory. this isolated strain was identified as mycobacterium phocaicum based on analysis of its 16s rrna gene sequence and its morphological, physiological, and biochemical properties. the strain was able to utilize fluoroglycofen ethyl as its sole source of carbon for growth and could degrade 100 mg l(-1) of fluoroglycofen ethyl to a non-detectable level within 72 h. the optimum temperature and ph fo ... | 2011 | 21424684 |
| microbial pathogens in source and treated waters from drinking water treatment plants in the united states and implications for human health. | an occurrence survey was conducted on selected pathogens in source and treated drinking water collected from 25 drinking water treatment plants (dwtps) in the united states. water samples were analyzed for the protozoa giardia and cryptosporidium (epa method 1623); the fungi aspergillus fumigatus, aspergillus niger and aspergillus terreus (quantitative pcr [qpcr]); and the bacteria legionella pneumophila (qpcr), mycobacterium avium, m. avium subspecies paratuberculosis, and mycobacterium intrace ... | 2016 | 27260619 |
| are we overlooking infections owing to non-tuberculous mycobacteria during routine conventional laboratory investigations? | a large number of potentially pathogenic non-tuberculous mycobacteria (ntm) encountered in the clinical laboratory makes it necessary to identify their species to ensure appropriate treatment. however, labor-intensive conventional methods of speciation are not used in every laboratory, and hence ntm infections are often ignored. polymerase chain reaction (pcr) restriction analysis (pra) was applied in this study for early identification and speciation of mycobacterial species on 306 cultures of ... | 2012 | 26785625 |
| mycobacterium phocaicum and mycobacterium avium-intracellulare in a patient with hot tub lung. | a 77-year-old woman who used her spa pool at least twice a day to relieve pain from osteoarthritis, developed progressive breathlessness, impaired pulmonary function, and radiographic changes consistent with hypersensitivity pneumonitis-like lung disease. m ycobacterium avium-intracellulare complex (mac) was cultured from bronchoalveolar lavage fluid. transbronchial biopsies revealed non-necrotizing granulomatous inflammation. sputum and spa pool water cultured m ycobacterium phocaicum but not m ... | 2015 | 25802744 |
| verrucous tumor mimicking squamous cell carcinoma in immunocompetent patient. | mycobacteria cause a range of diseases in both immunocompetent and immunosuppressed individuals. an increase in non-tuberculous mycobacterial (ntm) infections targeting skin has been described. many hypotheses have been developed in order to explain it: the increasing burden of immunocompromised individuals, immigration from endemic countries, improved laboratory identification techniques, and changes inhuman behavior that expose individuals to this ntm. mycobacterium mucogenicum group comprises ... | 2016 | 27267196 |
| diffuse pulmonary uptake of gallium-67 induced by pulmonary mycobacterium mucogenicum and mycobacterium phocaicum infection. | | 2017 | 28927855 |
| mycobacterium phocaicum bacteremia: an emerging infection in pediatric hematology-oncology patients. | nontuberculous mycobacteria may cause central venous catheter-associated bacteremia. between march 2011 and october 2013, 6 cases of mycobacterium phocaicum bacteremia were found in the pediatric hematology-oncology department. all patients recovered. no positive blood culture was documented after removal of the central venous catheter. all 4 patients with pulsed field gel electrophoresis had the same pattern, different from the water sample, suggesting a common water source. | 2014 | 25037036 |
| a case of catheter-related bloodstream infection caused by mycobacterium phocaicum. | we present a patient with double hit burkitt's like lymphoma who developed a catheter-related bloodstream infection due to mycobacterium phocaicum that was identified by rpob gene sequencing. his infection resolved with 7 weeks of antibiotics and port-a-cath removal. | 2013 | 23537787 |