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propagation of virulent and avirulent turkey hemorrhagic enteritis virus in cell culture.virulent and apathogenic isolates of turkey hemorrhagic enteritis virus (hev) were successfully propagated in lymphoblastoid cell lines of turkey origin, whereas spleen and kidney cell cultures from hev-infected turkeys failed to replicate the virus. the lymphoblastoid cell lines used were mdtc-rp16 and mdtc-rp19, which were previously established from tumors induced by marek's disease virus in turkeys. virus replication followed co-cultivation of lymphoblastoid cells with spleen cells from hev- ...19826297447
propagation of an avirulent turkey hemorrhagic enteritis virus isolate in chickens.a series of studies were undertaken to optimize the propagation of hemorrhagic enteritis virus (hev) in specific-pathogen-free (spf) chickens. a total of 562 spf chickens were orally inoculated with an australian avirulent hev isolate of turkey origin at 9, 14, 21, or 28 days of age with 5, 6, 7, or 8 log 10 genomic copies (gc), while 102 chickens served as uninfected controls. no clinical signs were observed in infected chickens. there was an inoculum-dose-dependent increase in the relative spl ...201829620462
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