| development of a polymerase chain reaction assay to detect cyprinid herpesvirus 2 in goldfish. | cyprinid herpesvirus 2 (cyhv2) has been associated with epidemic mortality among cultured populations of goldfish carassius auratus. as the principal target tissues are hematopoietic cells in the kidney and spleen, the disease is designated herpesviral hematopoietic necrosis (hvhn). originally described from japan, the virus is present in at least five other countries and probably has a global distribution in goldfish. preventing the further spread of the virus via control programs that exploit ... | 2009 | 19485127 |
| detection of cyprinid herpesvirus-3 (cyhv-3) dna in infected fish tissues by nested polymerase chain reaction. | diagnostic tests with high analytical sensitivity are required to detect cyprinid herpesvirus-3 (cyhv-3) in carriers that have been implicated in the dissemination of this important disease of koi and common carp cyprinus carpio. a nested polymerase chain reaction (pcr) test was developed to detect cyhv-3 dna in tissues of infected fish. the 2-round pcr amplified 529 and 379 bp segments of the cyhv-3 gene coding for the major capsid protein using specific external and internal primers, respectiv ... | 2007 | 18159669 |
| development of a pcr assay to detect cyprinid herpesvirus 1 in koi and common carp. | cyprinid herpesvirus 1 (cyhv1) infects all scaled and color varieties of common carp cyprinus carpio, including koi. while it is most often associated with unsightly growths known as 'carp pox,' the underlying lesion (epidermal hyperplasia) can arise from a variety of disease processes. cyhv1-induced epidermal hyperplasia may occur transiently in response to water temperature, and thus histopathology cannot be used in isolation to assess cyhv1 infection status. to address this problem, here we d ... | 2017 | 28177290 |
| coordinated and sequential transcription of the cyprinid herpesvirus-3 annotated genes. | cyprinid herpesvirus-3 (cyhv-3) is the cause of a fatal disease in carp and koi fish. the disease is seasonal and appears when water temperatures range from 18 to 28°c. cyhv-3 is a member of the alloherpesviridae, a family in the herpesvirales order that encompasses mammalian, avian and reptilian viruses. cyhv-3 is a large double-stranded dna (dsdna) herpesvirus with a genome of approximately 295kbp, divergent from other mammalian, avian and reptilian herpesviruses, but bearing several genes sim ... | 2012 | 22841491 |
| koi herpesvirus represents a third cyprinid herpesvirus (cyhv-3) in the family herpesviridae. | the sequences of four complete genes were analysed in order to determine the relatedness of koi herpesvirus (khv) to three fish viruses in the family herpesviridae: carp pox herpesvirus (cyprinid herpesvirus 1, cyhv-1), haematopoietic necrosis herpesvirus of goldfish (cyprinid herpesvirus 2, cyhv-2) and channel catfish virus (ictalurid herpesvirus 1, ichv-1). the genes were predicted to encode a helicase, an intercapsomeric triplex protein, the dna polymerase and the major capsid protein. the re ... | 2005 | 15914843 |
| concentrations of a koi herpesvirus (khv) in tissues of experimentally infected cyprinus carpio koi as assessed by real-time taqman pcr. | the koi herpesvirus (khv) is a herpes-like virus now recognized as a worldwide cause of mortality among populations of koi cyprinus carpio koi and common carp cyprinus carpio carpio. temperature is a key factor influencing virus replication both in cell culture and in the tissues of experimentally infected fish. genomic dna sequences were used to optimize a rapid real-time taqman pcr assay to detect and quantify khv dna as found in the tissues of virus-exposed fish. the assay allowed analytical ... | 2004 | 15521316 |
| detection of cyprinid herpesvirus 1 dna in cutaneous squamous cell carcinoma of koi carp (cyprinus carpio). | cutaneous squamous cell carcinoma (cscc) is an uncommon disease affecting koi carp (cyprinus carpio). cutaneous papilloma (carp pox) is a benign epidermal proliferation reported in koi and has been shown to be caused by cyprinid herpesvirus 1 (cyhv1). | 2018 | 28815758 |