Publications

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multiplex-pcr for identification of two species in genus hishimonus (hemiptera: cicadellidae) in jujube orchards.the insect family cicadellidae includes economically important vectors of plant pathogens. hishimonus sellatus (uhler) transmits jujube witches'-broom (jwb). currently, h. sellatus and hishimonus lamellatus cai et kuoh are observed to co-occur at the same locality on jujube. h. lamellatus is now suspected to be a jwb vector. as such, correct identification of hishimonus species present in vineyards is essential for epidemiological surveys. however, traditional identification of hishimonus by mor ...201526453733
identification of mulberry dwarf phytoplasmas in the genital organs and eggs of leafhopper hishimonoides sellatiformis.abstract the presence of mulberry dwarf (md) phytoplasmas in organs of the inoculative vector insects hishimonoides sellatiformis and hishimonus sellatus was determined by means of electron microscopy (em) and polymerase chain reaction (pcr) assays. many md phytoplasmas were detected in genital organs as well as in the intestines, salivary glands, brains, fat bodies, and thoracic ganglia of hishimonoides sellatiformis, but only in the intestine and salivary glands of hishimonus sellatus. many ph ...200018944513
'candidatus phytoplasma ziziphi', a novel phytoplasma taxon associated with jujube witches'-broom disease.phylogenetic relationships of five jujube witches'-broom (jwb) phytoplasma isolates from four different districts, and other phytoplasmas, were investigated by 16s rdna pcr amplification and sequence analysis. the 16s rdna sequences of any pair of the five isolates of jwb phytoplasmas were > 99.5% similar. the jwb phytoplasma 16s rdna sequences were most closely related to that of the elm yellows (ey) phytoplasma in 16s-group viii. phylogenetic analysis of the 16s rdna sequences from the jwb phy ...200312892123
two novel strains of wolbachia coexisting in both species of mulberry leafhoppers.wolbachia is an intracellular symbiont that causes reproductive disorders in many insects. its presence in the leafhoppers hishimonoides sellatiformis and hishimonus sellatus, vectors of mulberry dwarf-phytoplasma, was confirmed by the pcr analysis of 16s rdna, ftsz and wsp. sequencing of cloned pcr products revealed that two wolbachia strains coexist in both leafhoppers. the phylogenetic analysis of wsp revealed that these strains belong in novel positions in the b-group of wolbachia. these str ...200212421415
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