| isolation and characterisation of an unexpected class of insulinotropic peptides in the skin of the frog agalychnis litodryas. | skin secretions of the frog agalychnis litodryas were evaluated for the isolation and characterisation of novel insulinotropic peptides. crude secretions obtained from young adult frogs by mild electrical stimulation of the dorsal skin surface were purified by reverse-phase high-performance liquid chromatography (hplc) yielding 70 fractions. in acute 20-min incubations with glucose responsive brin-bd11 cells, fractions 39-42 (band 1) and fractions 44-46 (band 2) significantly stimulated insulin ... | 2004 | 15177918 |
| to hatch and hatch not: similar selective trade-offs but different responses to egg predators in two closely related, syntopic treefrogs. | risk-sensitive hatching is adaptive for species facing a trade-off between egg-stage and post-hatching risks, and environmental variation in one or both stages. such plasticity has been found in amphibians, fishes, reptiles and spiders, with red-eyed treefrogs (agalychnis callidryas) being the best-studied case. we assessed hatching plasticity and egg- and larval-stage risks in a closely related, syntopic species, the gliding leaf-frog (agalychnis spurrelli). we found a lower hatching response t ... | 2007 | 17375328 |
| radiographic and histological evidence of metabolic bone disease in gliding leaf frogs (agalychnis spurrelli). | bone alterations due to metabolic bone disease in captive animal populations can have a negative impact on repopulation and research initiatives. this investigation has the purpose of describing the principal radiographic and anatomopathological findings present in nine gliding leaf frogs (agalychnis spurrelli) kept in captivity with alterations in their spines and long bones. the observed histopathological findings were in the canalis vertebralis, paraspinal muscle and long bones, and included ... | 2019 | 30997428 |
| molecular modeling of four dermaseptin-related peptides of the gliding tree frog agalychnis spurrelli. | in this research, we present a preliminary computational study of four dermaseptin-related peptides from the skin exudate of the gliding tree frog agalychnis spurrelli. experimentally, the amino acid sequence of these peptides was elucidated through molecular cloning and tandem mass spectrometry and synthetic peptides were assayed against e. coli, s. aureus, and c. albicans to determine their antimicrobial properties. with the sequences on hand, a computational study of the structures was carrie ... | 2019 | 31422479 |
| unravelling the skin secretion peptides of the gliding leaf frog, agalychnis spurrelli (hylidae). | frog skin secretions contain medically-valuable molecules, which are useful for the discovery of new biopharmaceuticals. the peptide profile of the skin secretion of agalychnis spurrelli has not been investigated; therefore, the structural and biological characterization of its compounds signify an inestimable opportunity to acquire new biologically-active chemical scaffolds. in this work, skin secretion from this amphibian was analysed by molecular cloning and tandem mass spectrometry. although ... | 2019 | 31671555 |