molecular phylogeny of the pseudallescheria boydii species complex: proposal of two new species.pseudallescheria boydii (anamorph scedosporium apiospermum) is the species responsible for human scedosporiosis, a fungal infection with a high mortality rate and which is difficult to treat. recently, it has been demonstrated that high genetic variation exists within this species. we have performed a morphological and molecular study involving numerous strains of clinical or environmental origins and from different countries. the analysis of partial sequences of the beta-tubulin (two loci) and ...200516207945
antifungal susceptibilities of the species of the pseudallescheria boydii complex.eighty-four isolates belonging to eight species that constitute the pseudallescheria boydii complex were tested against 11 antifungal agents by using the microdilution method. there were significant differences among the species, with scedosporium aurantiacum being the most resistant. in general, voriconazole was the most active drug, followed by posaconazole.200617015631
prevalence and susceptibility testing of new species of pseudallescheria and scedosporium in a collection of clinical mold isolates.the prevalence of new species of pseudallescheria and scedosporium in a collection of 46 clinical isolates was analyzed. strain identification was done by morphological and molecular methods. four scedosporium aurantiacum isolates were detected among the panel of clinical strains. the susceptibility profile of s. aurantiacum was similar to that of scedosporium apiospermum.200717101671
successful treatment of scedosporium aurantiacum osteomyelitis in an immunocompetent patient.bacterial infections are a well-known complication of traumatic amputations. in cases involving contact with soil or water contaminated with manure, one also must be aware of infections with fungi, particularly scedosporium spp. we report on an immunocompetent trauma patient with an infection caused by a recently described scedosporium species, s. aurantiacum.200718171120
different virulence of the species of the pseudallescheria boydii complex.pseudallescheria boydii sensu lato is a complex of species involved in severe human infections. we have evaluated, using a murine model, the virulence of 2 strains of each of the most representative species of the complex, i.e., p. boydii sensu stricto, p. minutispora, scedosporium apiospermum, s. aurantiacum and s. dehoogii. we used two different inocula, i.e., 5 x 10(4) conidia/ml (for immunosuppressed animals) and 1 x 10(6) conidia/ml (for immunocompetent animals), which were administered int ...200918651312
population-based surveillance for scedosporiosis in australia: epidemiology, disease manifestations and emergence of scedosporium aurantiacum infection.australia-wide population-based surveillance for scedosporiosis identified 180 cases, with 118 (65.6%) cases of colonization and 62 (34.4%) cases of infection. predisposing factors for isolation of scedosporium spp. included chronic lung disease in 37.8% and malignancy in 21.7% of cases. predictors of invasive disease (n=62) included haematological stem cell transplantation (n=7), leukaemia (n=16) and diabetes mellitus (n=8). of 183 phenotypically-speciated isolates, 75 (41%) were scedosporium p ...200919549223
detection of occult scedosporium species in respiratory tract specimens from patients with cystic fibrosis by use of selective media.respiratory samples from cystic fibrosis outpatients were cultured on sabouraud's dextrose agar (sabd) containing antibiotics, mycosel, and scedosporium-selective medium (scesel+). thirty-two (14.7%) of 218 specimens from 11/69 (15.9%) patients yielded a scedosporium sp., most frequently scedosporium aurantiacum (17/218). scedosporium was recovered on scesel+, mycosel, and sabd from 90.6%, 50.0%, and 46.9% of the specimens tested, respectively.201019906904
experimental murine scedosporiosis: histopathology and azole treatment.the histopathology of clinical isolates of scedosporium apiospermum, scedosporium boydii, and scedosporium aurantiacum in immunosuppressed mice was evaluated. the organs most affected were the brain, kidneys, and spleen. s. aurantiacum produced more tissue damage than the other two species. amphotericin b (amb) was ineffective in the treatment of murine infections caused by such isolates, and posaconazole and voriconazole showed efficacy that correlated with the in vitro susceptibility data.201020547805
clinical associations and prevalence of scedosporium spp. in australian cystic fibrosis patients: identification of novel risk factors?risk factors for the association of scedosporium in cases of cystic fibrosis (cf) and its clinical implications are poorly understood. clinical, lung function and laboratory data of adult cf patients in sydney (april 2008-march 2009) were prospectively analysed for such risk factors. expectorated sputa were cultured for bacteria and examined for fungi using standard mycological and scedosporium-selective media, and by an internal transcribed spacer region-targeted multiplex pcr assay. scedospori ...201021067328
scedosporium aurantiacum is as virulent as s. prolificans, and shows strain-specific virulence differences, in a mouse model.several scedosporium species are clinically important emerging pathogens. scedosporium prolificans is reported to be the most virulent of the species, while the recently described species scedosporium aurantiacum, which accounts for a substantial proportion of australian clinical isolates is capable of causing a range of serious infections. in addition, environmental surveys have revealed a high prevalence of s. aurantiacum in the urban sydney region. this study was conducted to assess the virul ...201021067330
abundance of pseudallescheria/scedosporium species in the australian urban environment suggests a possible source for scedosporiosis including the colonization of airways in cystic fibrosis.members of the pseudallescheria/scedosporium species complex are emerging opportunistic fungal pathogens which have the capacity to colonize patients with damaged airways, including those with cystic fibrosis (cf). assuming human infection is acquired via inhalation of fungal spores from the environment, we performed a qualitative environmental survey encompassing 25 urban, semirural and rural sites in the greater sydney region to determine the prevalence of pseudallescheria/scedosporium species ...201021067333
identification of pseudallescheria and scedosporium species by three molecular methods.the major clinically relevant species in scedosporium (teleomorph pseudallescheria) are pseudallescheria boydii, scedosporium aurantiacum, scedosporium apiospermum, and scedosporium prolificans, while pseudallescheria minutispora, petriellopsis desertorum, and scedosporium dehoogii are exceptional agents of disease. three molecular methods targeting the partial β-tubulin gene were developed and evaluated to identify six closely related species of the s. apiospermum complex using quantitative rea ...201021177887
development and validation of a multiplex pcr for detection of scedosporium spp. in respiratory tract specimens from patients with cystic fibrosis.the emergence of scedosporium infections in diverse groups of individuals, which are often treatment refractory, warrants timely and accurate laboratory diagnosis. species- or group-specific primers based on internal transcribed spacer (its) sequence polymorphisms were designed for scedosporium aurantiacum, scedosporium dehoogii, scedosporium prolificans, pseudallescheria boydii species complex (former clade 5)/pseudallescheria apiosperma (formerly classified as s. apiospermum sensu lato) and ps ...201121325557
Activities of E1210 and Comparator Agents Tested by CLSI and EUCAST Broth Microdilution Methods against Fusarium and Scedosporium Species Identified Using Molecular Methods.Fusarium (n = 67) and Scedosporium (n = 63) clinical isolates were tested by two reference broth microdilution (BMD) methods against a novel broad-spectrum (active against both yeasts and molds) antifungal, E1210, and comparator agents. E1210 inhibits the inositol acylation step in glycophosphatidylinositol (GPI) biosynthesis, resulting in defects in fungal cell wall biosynthesis. Five species complex organisms/species of Fusarium (4 isolates unspeciated) and 28 Scedosporium apiospermum, 7 Scedo ...201222083469
pseudomonas aeruginosa inhibits the growth of scedosporium aurantiacum, an opportunistic fungal pathogen isolated from the lungs of cystic fibrosis patients.the filamentous fungus scedosporium aurantiacum and the bacterium pseudomonas aeruginosa are opportunistic pathogens isolated from lungs of the cystic fibrosis (cf) patients. p. aeruginosa has been known to suppress the growth of a number of cf related fungi such as aspergillus fumigatus, candida albicans, and cryptococcus neoformans. however, the interactions between p. aeruginosa and s. aurantiacum have not been investigated in depth. hence we assessed the effect of p. aeruginosa reference str ...201526379643
bactericidal and fungicidal activity of n-chlorotaurine is enhanced in cystic fibrosis sputum medium.lung infections with multiresistant pathogens are a major problem of patients suffering from cystic fibrosis (cf). n-chlorotaurine (nct), a microbicidal active chlorine compound with no resistance development, is well tolerated upon inhalation. the aim of this study was to investigate nct on its bactericidal and fungicidal activity in vitro in artificial sputum medium (asm) mimicking the composition of cystic fibrosis mucus.the medium was inoculated with bacteria (staphylococcus aureus including ...201728223376
cyclophilin and enolase are the most prevalent conidial antigens of lomentospora prolificans recognized by healthy human salivary iga and cross-react with aspergillus fumigatus.the study of the immunocompetent airways immune response may provide important information to improve the therapeutic efficacy against lomentospora (scedosporium) prolificans. so, this study aimed to identify the most prevalent conidial antigens of this multiresistant fungus recognized by healthy human salivary immunoglobulin a, and to study their expression and cross-reactivity with other fungal species.201627485921
[using galleria mellonella as an in vivo model to study the virulence of some bacterial and fungal agents].non-vertebrate hosts, such as galleria mellonella, namely wax moth, have been used to study microbial virulence and host defense. this organism has advantages as it is economical, ethically expedient and easy to handle. here we describe an experimental in vivo study using the larvae of galleria mellonella infected with some bacterial and fungal pathogens. in this study, extended-spectrum beta-lactamase (esbl) producing and non-producing escherichia coli, klebsiella pneumoniae and pseudomonas aer ...201526313278
effect of water activity and titania p25 photocatalyst on inactivation of pathogenic fungi--contribution to the protection of public health.the aim of this study was to determine the antifungal activity of titanium dioxide activated by indoor light on czapek yeast agar (cya).201526615661
structural analysis of glucosylceramides (glccer) from species of the pseudallescheria/scedosporium complex.glucosylceramides (glccer) are the main neutral glycosphingolipids expressed in fungal cells. in this work, glucosylceramides (glccer) were extracted from three strains of scedosporium (pseudallescheria) boydii, one strain of pseudallescheria ellipsoidea and one strain of pseudallescheria angusta and purified by several chromatographic steps. using high-performance thin layer chromatography (hptlc), we found a similarity between glccer obtained from all of the analysed strains. a detailed struct ...201626781373
draft genome of australian environmental strain wm 09.24 of the opportunistic human pathogen scedosporium aurantiacum.we report here the first genome assembly and annotation of the human-pathogenic fungus scedosporium aurantiacum, with a predicted 10,525 genes, and 11,661 transcripts. the strain wm 09.24 was isolated from the environment at circular quay, sydney, new south wales, australia.201525676755
phenotypic profiling of scedosporium aurantiacum, an opportunistic pathogen colonizing human lungs.genotyping studies of australian scedosporium isolates have revealed the strong prevalence of a recently described species: scedosporium aurantiacum. in addition to occurring in the environment, this fungus is also known to colonise the respiratory tracts of cystic fibrosis (cf) patients. a high throughput phenotype microarray (pm) analysis using 94 assorted substrates (sugars, amino acids, hexose-acids and carboxylic acids) was carried out for four isolates exhibiting different levels of virule ...201525811884
secretion of proteases by an opportunistic fungal pathogen scedosporium aurantiacum.scedosporium aurantiacum is an opportunistic filamentous fungus increasingly isolated from the sputum of cystic fibrosis patients, and is especially prevalent in australia. at the moment, very little is known about the infection mechanism of this fungus. secreted proteases have been shown to contribute to fungal virulence in several studies with other fungi. here we have compared the profiles of proteases secreted by a clinical isolate scedosporium aurantiacum (wm 06.482) and an environmental st ...201728060882
distribution of the different species of the pseudallescheria boydii/scedosporium apiospermum complex in french patients with cystic various new sibling species within the pseudallescheria boydii/scedosporium apiospermum complex have been described recently with differences in their susceptibility to antifungals, this study was conducted in order to determine their respective frequency in cystic fibrosis. results indicated that p. boydii largely predominated (62%), followed by s. apiospermum (24%), scedosporium aurantiacum (10%) and pseudallescheria minutispora (4%). scedosporium dehoogii was not recovered in this study. t ...201323461512
human-impacted areas of france are environmental reservoirs of the pseudallescheria boydii/scedosporium apiospermum species complex.species of the pseudallescheria boydii/scedosporium apiospermum complex (psc) are emerging fungal pathogens able to chronically colonize the airways of patients with cystic fibrosis (cf). as p. boydii was found more frequently colonizing the lungs of cf patients in france than in other european countries in a previous report, the present study was conducted in order to clarify distribution of psc species in france and to characterize their natural habitat. the highest densities of psc isolates w ...201524684308
semi-automated repetitive sequence-based pcr amplification for species of the scedosporium apiospermum complex.the scedosporium apiospermum species complex usually ranks second among the filamentous fungi colonizing the airways of patients with cystic fibrosis (cf), but little is known about the molecular epidemiology of the airway colonization.201626486722
susceptibility and diversity in the therapy-refractory genus scedosporium.scedosporium species show decreased susceptibility to the majority of systemic antifungal drugs. acquired resistance is likely to disseminate differentially with the mode of exchange of genetic material between lineages. inter- and intraspecific diversities of scedosporium species were analyzed for three partitions (rdna internal transcribed spacer gene [its], partial β-tubulin gene, and amplified fragment length polymorphism profiles), with the aim to establish distribution of resistance betwee ...201425070092
the 'species complex' issue in clinically relevant fungi: a case study in scedosporium apiospermum.the genus scedosporium currently comprises six species, scedosporium apiospermum, scedosporium boydii, pseudallescheria angusta, scedosporium minutisporum, scedosporium dehoogii, and scedosporium aurantiacum, most of which can be distinguished with the primary fungal dna barcode, the its1/2 region of the rdna gene cluster. in the present study, four additional genetic loci were explored from a phylogenetic point of view enabling a barcoding approach based on k2p pairwise distances to resolve the ...201626781369
intra- and interspecific diversity of ultrastructural markers in scedosporium.ultrastructural features of conidia, lateral walls of aerial and submerged hyphal cells, and of septal pore apparatus of scedosporium apiospermum, s. boydii, pseudallescheria angusta and scedosporium aurantiacum were studied. submerged hyphal cells possessed a thick extracellular matrix. crystalline satellites accessory to the septal pore apparatus were revealed. fundamental ultrastructural features appeared to be heterogeneous at low taxonomic levels. the closely interrelated members of the s. ...201626781370
scedosporium aurantiacum brain abscess after near-drowning in a survivor of a tsunami in japan.many victims of the tsunami that occurred following the great east japan earthquake on march 11, 2011 developed systemic disorders owing to aspiration pneumonia. herein, we report a case of tsunami lung wherein scedosporium aurantiacum was detected in the respiratory tract. a magnetic resonance image of the patient's head confirmed multiple brain abscesses and lateral right ventricle enlargement. in this case report, we describe a potential refractory multidrug-resistant infection following a ts ...201324238227
matrix-assisted laser desorption ionization-time of flight mass spectrometry for fast and accurate identification of pseudallescheria/scedosporium increasing number of infections due to pseudallescheria/scedosporium species has been reported during the past decades, both in immunocompromised and immunocompetent patients. additionally, these fungi are now recognized worldwide as common agents of fungal colonization of the airways in cystic fibrosis patients, which represents a risk factor for disseminated infections after lung transplantation. currently six species are described within the pseudallescheria/scedosporium genus, including s ...201424476388
dihydroorotate dehydrogenase inhibitor f901318 has potent in vitro activity against scedosporium species and lomentospora prolificans.scedosporium species and lomentospora prolificans are increasing causes of invasive infections in immunocompromised hosts and many isolates are resistant to available antifungals. our objective was to assess the in vitro potency of f901318, a member of the orotomide class of antifungals, against scedosporium species and l. prolificans .201728333310
changes in the epidemiological landscape of invasive mould infections and disease.although a wide variety of pathogens are associated with invasive mould diseases, aspergillus spp. have historically been one of the most common causative organisms. most invasive mould infections are caused by members of the aspergillus fumigatus species complex and an emerging issue is the occurrence of azole resistance in a. fumigatus, with resistance to amphotericin b documented in other aspergillus spp. the epidemiology of invasive fungal disease has shifted in recent years as non-a. fumiga ...201728355462
the effect of anatase and rutile crystallites isolated from titania p25 photocatalyst on growth of selected mould fungi.antifungal properties of anatase and rutile crystallites isolated from commercial titania p25 photocatalyst were investigated by mycelium growth in the dark and under indoor light. investigated fungi, i.e., pseudallescheria boydii, scedosporium apiospermum, pseudallescheria ellipsoidea, scedosporium aurantiacum, aspergillus versicolor, aspergillus flavus, stachybotrys chartarum, penicillium chrysogenum, aspergillus melleus, were isolated from air and from moisture condensed on walls. anatase and ...201526177513
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