| molecular phylogeny of the pseudallescheria boydii species complex: proposal of two new species. | pseudallescheria boydii (anamorph scedosporium apiospermum) is the species responsible for human scedosporiosis, a fungal infection with a high mortality rate and which is difficult to treat. recently, it has been demonstrated that high genetic variation exists within this species. we have performed a morphological and molecular study involving numerous strains of clinical or environmental origins and from different countries. the analysis of partial sequences of the beta-tubulin (two loci) and ... | 2005 | 16207945 |
| identification of pseudallescheria and scedosporium species by three molecular methods. | the major clinically relevant species in scedosporium (teleomorph pseudallescheria) are pseudallescheria boydii, scedosporium aurantiacum, scedosporium apiospermum, and scedosporium prolificans, while pseudallescheria minutispora, petriellopsis desertorum, and scedosporium dehoogii are exceptional agents of disease. three molecular methods targeting the partial β-tubulin gene were developed and evaluated to identify six closely related species of the s. apiospermum complex using quantitative rea ... | 2010 | 21177887 |
| development and validation of a multiplex pcr for detection of scedosporium spp. in respiratory tract specimens from patients with cystic fibrosis. | the emergence of scedosporium infections in diverse groups of individuals, which are often treatment refractory, warrants timely and accurate laboratory diagnosis. species- or group-specific primers based on internal transcribed spacer (its) sequence polymorphisms were designed for scedosporium aurantiacum, scedosporium dehoogii, scedosporium prolificans, pseudallescheria boydii species complex (former clade 5)/pseudallescheria apiosperma (formerly classified as s. apiospermum sensu lato) and ps ... | 2011 | 21325557 |
| Rapid identification of pseudallescheria and scedosporium strains by using rolling circle amplification. | The Pseudallescheria boydii complex, comprising environmental pathogens with Scedosporium anamorphs, has recently been subdivided into five main species: Scedosporium dehoogii, S. aurantiacum, Pseudallescheria minutispora, P. apiosperma, and P. boydii, while the validity of some other taxa is being debated. Several Pseudallescheria and Scedosporium species are indicator organisms of pollution in soil and water. Scedosporium dehoogii in particular is enriched in soils contaminated by aliphatic hy ... | 2012 | 22057865 |
| bactericidal and fungicidal activity of n-chlorotaurine is enhanced in cystic fibrosis sputum medium. | lung infections with multiresistant pathogens are a major problem of patients suffering from cystic fibrosis (cf). n-chlorotaurine (nct), a microbicidal active chlorine compound with no resistance development, is well tolerated upon inhalation. the aim of this study was to investigate nct on its bactericidal and fungicidal activity in vitro in artificial sputum medium (asm) mimicking the composition of cystic fibrosis mucus.the medium was inoculated with bacteria (staphylococcus aureus including ... | 2017 | 28223376 |
| distribution of the different species of the pseudallescheria boydii/scedosporium apiospermum complex in french patients with cystic fibrosis. | as various new sibling species within the pseudallescheria boydii/scedosporium apiospermum complex have been described recently with differences in their susceptibility to antifungals, this study was conducted in order to determine their respective frequency in cystic fibrosis. results indicated that p. boydii largely predominated (62%), followed by s. apiospermum (24%), scedosporium aurantiacum (10%) and pseudallescheria minutispora (4%). scedosporium dehoogii was not recovered in this study. t ... | 2013 | 23461512 |
| matrix-assisted laser desorption ionization-time of flight mass spectrometry for fast and accurate identification of pseudallescheria/scedosporium species. | an increasing number of infections due to pseudallescheria/scedosporium species has been reported during the past decades, both in immunocompromised and immunocompetent patients. additionally, these fungi are now recognized worldwide as common agents of fungal colonization of the airways in cystic fibrosis patients, which represents a risk factor for disseminated infections after lung transplantation. currently six species are described within the pseudallescheria/scedosporium genus, including s ... | 2014 | 24476388 |
| reverse line blot hybridisation screening of pseudallescheria/scedosporium species in patients with cystic fibrosis. | the pcr-rlb (reverse line blot hybridisation) was applied as a molecular technique for the detection of members of pseudallescheria and scedosporium from sputum of patients with cystic fibrosis (cf). fifty-nine sputum samples were collected from 52 cf patients, which were analysed by culture and pcr-rlb. conventional and semi-selective culture yielded five positive samples, but the pcr-rlb hybridisation assay permitted the detection of members of pseudallescheria/scedosporium in 32 out of 52 pat ... | 2011 | 21995657 |
| human-impacted areas of france are environmental reservoirs of the pseudallescheria boydii/scedosporium apiospermum species complex. | species of the pseudallescheria boydii/scedosporium apiospermum complex (psc) are emerging fungal pathogens able to chronically colonize the airways of patients with cystic fibrosis (cf). as p. boydii was found more frequently colonizing the lungs of cf patients in france than in other european countries in a previous report, the present study was conducted in order to clarify distribution of psc species in france and to characterize their natural habitat. the highest densities of psc isolates w ... | 2015 | 24684308 |
| susceptibility and diversity in the therapy-refractory genus scedosporium. | scedosporium species show decreased susceptibility to the majority of systemic antifungal drugs. acquired resistance is likely to disseminate differentially with the mode of exchange of genetic material between lineages. inter- and intraspecific diversities of scedosporium species were analyzed for three partitions (rdna internal transcribed spacer gene [its], partial β-tubulin gene, and amplified fragment length polymorphism profiles), with the aim to establish distribution of resistance betwee ... | 2014 | 25070092 |
| the 'species complex' issue in clinically relevant fungi: a case study in scedosporium apiospermum. | the genus scedosporium currently comprises six species, scedosporium apiospermum, scedosporium boydii, pseudallescheria angusta, scedosporium minutisporum, scedosporium dehoogii, and scedosporium aurantiacum, most of which can be distinguished with the primary fungal dna barcode, the its1/2 region of the rdna gene cluster. in the present study, four additional genetic loci were explored from a phylogenetic point of view enabling a barcoding approach based on k2p pairwise distances to resolve the ... | 2016 | 26781369 |
| critical tests for determination of microbiological quality and biological activity in commercial vermicompost samples of different origins. | the aim of the present paper was to show that differences in biological activity among commercially produced vermicompost samples can be found by using a relatively simple test system consisting of microorganism tests on six microbiological media and soilless seedling growth tests with four vegetable crop species. significant differences in biological properties among analyzed samples were evident both at the level of microbial load as well as plant growth-affecting activity. these differences w ... | 2013 | 23504062 |