| shewanella pneumatophori sp. nov., an eicosapentaenoic acid-producing marine bacterium isolated from the intestines of pacific mackerel (pneumatophorus japonicus). | an eicosapentaenoic acid-producing bacterium, previously described as shewanella sp. strain scrc-2738, was classified by phenotypic characterization, chemotaxonomic analysis, 16s rrna gene sequence analysis and dna-dna hybridization. the isolate was gram-negative, rod-shaped and motile by using polar flagella. the strain grew at 4-32 degrees c; the optimum growth temperature was 27 degrees c. nacl was required for growth. the major isoprenoid quinones were ubiquinone-7 and ubiquinone-8 and its d ... | 2005 | 16280496 |
| a phosphopantetheinyl transferase gene essential for biosynthesis of n-3 polyunsaturated fatty acids from moritella marina strain mp-1. | a phosphopantetheinyl transferase (pptase) gene (pfae), cloned from the docosahexaenoic acid (dha)-producing bacterium moritella marina strain mp-1, has an open reading frame of 861 bp encoding a 287-amino acid protein. when the pfae gene was expressed with pfaa-d, which are four out of five essential genes for biosynthesis of eicosapentaenoic acid (epa) derived from shewanella pneumatophori scrc-2738 in escherichia coli, the recombinant produced 12% epa of total fatty acids. this suggests that ... | 2006 | 16859689 |
| pfab products determine the molecular species produced in bacterial polyunsaturated fatty acid biosynthesis. | when pdha4, a vector carrying all five pfaa-pfae genes responsible for docosahexaenoic acid (dha; 22:6) biosynthesis in moritella marina mp-1, was coexpressed in escherichia coli with the individual pfaa-pfad genes for eicosapentaenoic acid (epa; 20:5) biosynthesis from shewanella pneumatophori scrc-2738, both polyunsaturated fatty acids were synthesized only in the recombinant carrying pfab for epa synthesis. escherichia coli coexpressing a deleted construct comprising pfaa, pfac, pfad and pfae ... | 2009 | 19453514 |
| the escherichia coli highly expressed entd gene complements the pfae deficiency in a pfa gene clone responsible for the biosynthesis of long-chain n-3 polyunsaturated fatty acids. | the escherichia coli entd gene, which encodes an sfp-type phosphopantetheinyl transferase (pptase) that is involved in the biosynthesis of siderophore, is available as a high-expression aska clone (pca24n::entd) constructed from the e. coli k-12 strain ag1. in e. coli dh5alpha, pca24n::entd complemented a pfae-deficient clone that comprised pfaa, pfab, pfac and pfad, which are four of the five pfa genes that are responsible for the biosynthesis of eicosapentaenoic acid derived from shewanella pn ... | 2010 | 20636978 |