| molecular characterization of a virus from the family luteoviridae associated with cotton blue disease. | cotton blue disease is an aphid-transmitted cotton disease described in brazil in 1962 as vein mosaic "var. ribeirão bonito". at present it causes economically important losses in cotton crops if control measures are not implemented. the observed symptoms and mode of transmission have prompted researchers to speculate that cotton blue disease could be attributed to a member of the family luteoviridae, but there was no molecular evidence supporting this hypothesis. we have amplified part of the g ... | 2005 | 15789270 |
| widespread distribution and a new recombinant species of brazilian virus associated with cotton blue disease. | cotton blue disease (cbd), an important global cotton crop pathology responsible for major economic losses, is prevalent in the major cotton-producing states of brazil. typical cbd symptoms include stunting due to internodal shortening, leaf rolling, intense green foliage, and yellowing veins. atypical cbd symptoms, including reddish and withered leaves, were also observed in brazilian cotton fields in 2007. recently, a polerovirus named cotton leafroll dwarf virus (clrdv) was shown to be associ ... | 2008 | 18937850 |
| the complete genome sequence of a virus associated with cotton blue disease, cotton leafroll dwarf virus, confirms that it is a new member of the genus polerovirus. | cotton blue disease is the most important virus disease of cotton in the southern part of america. the complete nucleotide sequence of the ssrna genome of the cotton blue disease-associated virus was determined for the first time. it comprised 5,866 nucleotides, and the deduced genomic organization resembled that of members of the genus polerovirus. sequence homology comparison and phylogenetic analysis confirm that this virus (previous proposed name cotton leafroll dwarf virus) is a member of a ... | 2010 | 20677026 |
| Profile of small interfering RNAs from cotton plants infected with the polerovirus Cotton leafroll dwarf virus. | In response to infection, viral genomes are processed by Dicer-like (DCL) ribonuclease proteins into viral small RNAs (vsRNAs) of discrete sizes. vsRNAs are then used as guides for silencing the viral genome. The profile of vsRNAs produced during the infection process has been extensively studied for some groups of viruses. However, nothing is known about the vsRNAs produced during infections of members of the economically important family Luteoviridae, a group of phloem-restricted viruses. Here ... | 2011 | 21864377 |
| agroinoculation of a full-length cdna clone of cotton leafroll dwarf virus (clrdv) results in systemic infection in cotton and the model plant nicotiana benthamiana. | cotton blue disease is the most important viral disease of cotton in the southern part of south america. its etiological agent, cotton leafroll dwarf virus (clrdv), is specifically transmitted to host plants by the aphid vector (aphis gossypii) and any attempt to perform mechanical inoculations of this virus into its host has failed. this limitation has held back the study of this virus and the disease it causes. in this study, a full-length cdna of clrdv was constructed and expressed in vivo un ... | 2013 | 23623981 |
| identification of a new cotton disease caused by an atypical cotton leafroll dwarf virus in argentina. | an outbreak of a new disease occurred in cotton (gossypium hirsutum) fields in northwest argentina starting in the 2009-10 growing season and is still spreading steadily. the characteristic symptoms of the disease included slight leaf rolling and a bushy phenotype in the upper part of the plant. in this study, we determined the complete nucleotide sequences of two independent virus genomes isolated from cotton blue disease (cbd)-resistant and -susceptible cotton varieties. this virus genome comp ... | 2017 | 28035870 |
| complete genome sequences of two new virus isolates associated with cotton blue disease resistance breaking in brazil. | since 2006, brazilian cotton (gossypium hirsutum) crops planted with cultivars that are resistant to cotton blue disease have developed a new disease termed "atypical" cotton blue disease or atypical vein mosaic disease. here, we describe the complete genomes of two virus isolates associated with this disease. the new virus isolates, called clrdv-acr3 and clrdv-ima2, were found to have a high degree of nucleotide and amino acid sequence similarity to previously described isolates of cotton leafr ... | 2015 | 25772571 |
| global alteration of micrornas and transposon-derived small rnas in cotton (gossypium hirsutum) during cotton leafroll dwarf polerovirus (clrdv) infection. | small rnas (srnas) are a class of non-coding rnas ranging from 20- to 40-nucleotides (nts) that are present in most eukaryotic organisms. in plants, srnas are involved in the regulation of development, the maintenance of genome stability and the antiviral response. viruses, however, can interfere with and exploit the silencing-based regulatory networks, causing the deregulation of srnas, including small interfering rnas (sirnas) and micrornas (mirnas). to understand the impact of viral infection ... | 2012 | 22987114 |
| genetic similarity between cotton leafroll dwarf virus and chickpea stunt disease associated virus in india. | the cotton leafroll dwarf virus (clrdv) is one of the most devastating pathogens of cotton. this malady, known as cotton blue disease, is widespread in south america where it causes huge crop losses. recently the disease has been reported from india. we noticed occurrence of cotton blue disease and chickpea stunt disease in adjoining cotton and chickpea fields and got interested in knowing if these two viral diseases have some association. by genetic studies, we have shown here that clrdv is ver ... | 2016 | 27904466 |
| micrornas as reference genes for quantitative pcr in cotton. | cotton (gossypium hirsutum) is the most important non-food plant in the world. studies concerning the fiber quality and plant fitness of cotton at molecular level depend on high sensitive and reproducible gene-expression assays. however, only a few reports have described genes suitable for normalizing gene expression data. in this study, we report for the first time that micrornas (mirnas) are reliable reference genes (rgs) for cotton gene expression normalization in quantitative real-time rever ... | 2017 | 28414734 |
| function and diversity of p0 proteins among cotton leafroll dwarf virus isolates. | the rna silencing pathway is an important anti-viral defense mechanism in plants. as a counter defense, some members of the viral family luteoviridae are able to evade host immunity by encoding the p0 rna silencing suppressor protein. here we explored the functional diversity of p0 proteins among eight cotton leafroll dwarf virus (clrdv) isolates, a virus associated with a worldwide cotton disease known as cotton blue disease (cbd). | 2015 | 26260343 |
| the p0 protein encoded by cotton leafroll dwarf virus (clrdv) inhibits local but not systemic rna silencing. | plants employ rna silencing as a natural defense mechanism against viruses. as a counter-defense, viruses encode silencing suppressor proteins (ssps) that suppress rna silencing. most, but not all, the p0 proteins encoded by poleroviruses have been identified as ssp. in this study, we demonstrated that cotton leafroll dwarf virus (clrdv, genus polerovirus) p0 protein suppressed local silencing that was induced by sense or inverted repeat transgenes in agrobacterium co-infiltration assay in nicot ... | 2014 | 24370867 |