| an ophiovirus isolated from lettuce with big-vein symptoms. | big-vein is a widespread and damaging disease of lettuce, transmitted through soil by the chytrid fungus olpidium brassicae, and generally supposed to be caused by lettuce big-vein virus (lbvv; genus varicosavirus). this virus is reported to have rigid rod-shaped particles, a divided double-stranded rna genome, and one capsid protein of 48 kd, but has not been isolated or rigorously shown to cause the disease. we provide evidence that a totally different virus, here named mirafiori lettuce virus ... | 2000 | 11205109 |
| nucleotide sequence of the coat protein gene of lettuce big-vein virus. | a sequence of 1425 nt was established that included the complete coat protein (cp) gene of lettuce big-vein virus (lbvv). the lbvv cp gene encodes a 397 amino acid protein with a predicted m(r) of 44486. antisera raised against synthetic peptides corresponding to n-terminal or c-terminal parts of the lbvv cp reacted in western blot analysis with a protein with an m(r) of about 48000. rna extracted from purified particles of lbvv by using proteinase k, sds and phenol migrated in gels as two singl ... | 2001 | 11369898 |
| the nucleotide sequence of rna1 of lettuce big-vein virus, genus varicosavirus, reveals its relation to nonsegmented negative-strand rna viruses. | the complete nucleotide sequence of rna1 from lettuce big-vein virus (lbvv), the type member of the genus varicosavirus, was determined. lbvv rna1 consists of 6797 nucleotides and contains one large orf that encodes a large (l) protein of 2040 amino acids with a predicted m(r) of 232,092. northern blot hybridization analysis indicated that the lbvv rna1 is a negative-sense rna. database searches showed that the amino acid sequence of l protein is homologous to those of l polymerases of nonsegmen ... | 2002 | 12083827 |
| relationship between the lettuce big-vein virus and its vector, olpidium brassicae. | | 1962 | 13876064 |
| nucleotide sequence of rna2 of lettuce big-vein virus and evidence for a possible transcription termination/initiation strategy similar to that of rhabdoviruses. | lettuce big-vein virus (lbvv) is the type species of the genus varicosavirus and is a two-segmented negative-sense single-stranded rna virus. the larger lbvv genome segment (rna1) consists of 6797 nt and encodes an l polymerase that resembles that of rhabdoviruses. here, the nucleotide sequence of the second lbvv genome segment (rna2) is reported. lbvv rna2 consisted of 6081 nt and contained antisense information for five major orfs: orf1 (nt 210-1403 on the viral rna), orf2 (nt 1493-2494), orf3 ... | 2004 | 15302964 |
| the complete nucleotide sequence of a spanish isolate of citrus psorosis virus: comparative analysis with other ophioviruses. | the complete genomic sequence (11278 nt) of citrus psorosis virus (cpsv), isolate p-121 from spain, was determined and compared with those from isolate cpv-4 and from other ophioviruses. the three rnas of p-121 had similar size and identical organization as those of cpv-4. the 24k and the rdrp proteins were potentially encoded in the viral complementary (vc) strand of rna 1, the 54k protein potentially encoded in vcrna 2 and the coat protein encoded in vcrna 3. these four proteins from p-121 and ... | 2005 | 15449144 |
| genetic variability in the coat protein genes of lettuce big-vein associated virus and mirafiori lettuce big-vein virus. | available data suggests that lettuce big-vein disease is caused by the ophiovirus mirafiori lettuce big-vein virus (mlbvv) but not by the varicosavirus lettuce big-vein-associated virus (lbvav), although the latter is frequently associated with the disease. since the disease occurs worldwide, the putative coat protein (cp) open reading frames of geographically distinct isolates of mlbvv and lbvav were sequenced. comparison of both nucleotide and amino acid sequences showed a high level of sequen ... | 2004 | 15614432 |
| molecular analysis of coat protein coding region of tobacco stunt virus shows that it is a strain of lettuce big-vein virus in the genus varicosavirus. | to evaluate the relationship between tobacco stunt virus (tstv) and lettuce big-vein virus (lbvv), we determined nucleotide sequences of the coat protein (cp) coding region of five tstv and three lbvv isolates and compared them with those of one japanese and four spanish isolates of lbvv. cp coding regions were identical in size and the nucleotide and amino acid sequence identities between tstv and lbvv were 95.6-96.5% and 97.2-98.7%, respectively. phylogenetic analysis of nucleotide sequences i ... | 2005 | 15645375 |
| comparative infection progress analysis of lettuce big-vein virus and mirafiori lettuce virus in lettuce crops by developed molecular diagnosis techniques. | abstract nonisotopic molecular dot blot hybridization technique and multiplex reverse transcription-polymerase chain reaction assay for the specific detection of lettuce big-vein virus (lbvv) and mirafiori lettuce virus (milv) in lettuce tissue were developed. both procedures were suitable for the specific detection of both viruses in a range of naturally infected lettuce plants from various spanish production areas and seven different cultivars. the study of the distribution of both viruses in ... | 2004 | 18943765 |
| transmission by olpidium brassicae of mirafiori lettuce virus and lettuce big-vein virus, and their roles in lettuce big-vein etiology. | abstract big-vein disease occurs on lettuce worldwide in temperate conditions; the causal agent has been presumed to be lettuce big-vein virus (lbvv), genus varicosavirus, vectored by the soilborne fungus olpidium brassicae. recently, the role of lbvv in the etiology of big-vein disease has been questioned because a second soilborne virus, mirafiori lettuce virus (milv), genus ophiovirus, has been found frequently in big-vein-affected lettuce. lbvv and milv, detectable and distinguishable by enz ... | 2002 | 18944001 |
| further evidence of mirafiori lettuce big-vein virus but not of lettuce big-vein associated virus with big-vein disease in lettuce. | mirafiori lettuce big-vein virus (mlbvv) and lettuce big-vein associated virus (lbvav) are found in association with big-vein disease of lettuce. discrimination between the two viruses is critical for elucidating the etiology of big-vein disease. using specific antibodies to mlbvv and lbvav for western blotting and exploiting differences between mlbvv and lbvav in host reaction of cucumber and temperature dependence in lettuce, we separated the two viruses by transfering each virus from doubly i ... | 2008 | 18944196 |
| comparison of the coat protein genes of lettuce big-vein associated virus isolates from australia with those of isolates from other continents. | the complete coat protein (cp) nucleotide sequences of seven lettuce big-vein associated virus (lbvav) isolates from australia were compared to those of 22 other lbvav and five tobacco stunt virus (tstv) isolates. on phylogenetic analysis, clade i contained only lbvav isolates from europe, sub-clade iia only australian lbvav isolates, iib only japanese lbvav isolates, and iic only tstv isolates from japan. in the amino acid sequences deduced, the central region of the gene was most divergent. me ... | 2010 | 20232088 |
| comparison of the coat protein genes of mirafiori lettuce big-vein virus isolates from australia with those of isolates from other continents. | the complete coat protein nucleotide encoding sequences of 13 mirafiori lettuce big-vein virus isolates from australia were compared to those of 23 other isolates, including one from australia. on phylogenetic analysis, sub-clade a1 contained isolates from australia (13), europe and japan, a2 contained isolates from australia (1), europe and south america, and b1 and b2 contained only european isolates. in the amino acid sequences deduced, the n-terminus and central regions varied considerably b ... | 2010 | 20635189 |