| molecular characterization of dsrna segments 2 and 5 and electron microscopy of a novel reovirus from a hypovirulent isolate, w370, of the plant pathogen rosellinia necatrix. | a hypovirulent isolate, w370, of the white root rot fungus rosellinia necatrix has previously been shown to harbour 12 dsrna segments. in this study, complete nucleotide sequences of segments 2 and 5 of w370 dsrnas were determined. the nucleotide sequence of genome segment 2 was 3773 bases long with a single long open reading frame (orf) encoding 1226 amino acid residues with a predicted molecular mass of approximately 138.5 kda. the nucleotide sequence of segment 5 was 2089 bases long with a si ... | 2003 | 12917464 |
| complete nucleotide sequences of genome segments 1 and 3 of rosellinia anti-rot virus in the family reoviridae. | the nucleotide sequences of genome segments 1 and 3 of rosellinia anti-rot virus (rarv) from a hypovirulent isolate, w370, of the plant pathogen rosellinia necatrix were determined. the complete nucleotide sequence of the genome segment 1 encoded a putative rna-dependent rna polymerase (rdrp). the deduced amino acid sequence of rdrp of rarv showed 29% identity with rdrps of colorado tick fever virus (ctfv) and european eyach virus (eyav) in the genus coltivirus, and identities of 23-21% with mem ... | 2004 | 15045563 |
| complete genome sequence of mycoreovirus-1/cp9b21, a member of a novel genus within the family reoviridae, isolated from the chestnut blight fungus cryphonectria parasitica. | mycoreovirus 1 (myrv-1) is the type species of the newly described genus mycoreovirus of the large virus family reoviridae. the virus was isolated from a hypovirulent strain (9b21) of the chestnut blight fungus, cryphonectria parasitica. a previous study showed that double-shelled particles introduced to fungal spheroplasts resulted in stably infected colonies. of the 11 double-stranded rna genomic segments (s1-s11), the three largest (s1-s3) were sequenced previously and shown to have moderate ... | 2004 | 15483262 |
| a mycoreovirus suppresses rna silencing in the white root rot fungus, rosellinia necatrix. | rna silencing is a fundamental antiviral response in eukaryotic organisms. we investigated the counterdefense strategy of a fungal virus (mycovirus) against rna silencing in the white root rot fungus, rosellinia necatrix. we generated an r. necatrix strain that constitutively induced rna silencing of the exogenous green fluorescent protein (gfp) gene, and infected it with each of four unrelated mycoviruses, including a partitivirus, a mycoreovirus, a megabirnavirus, and a quadrivirus. infection ... | 2013 | 23896640 |
| artificial infection of rosellinia necatrix with purified viral particles of a member of the genus mycoreovirus reveals its uneven distribution in single colonies. | abstract rosellinia necatrix mycoreovirus 3 (w370) (rnmyrv-3/w370, described as rnmyrv-3 in this paper), a member of the newly established genus mycoreovirus within the family reoviridae, is the hypovirulence factor of the white root rot fungus, rosellinia necatrix. two virus-free fungal isolates (w37 and w97) that were somatically incompatible with the virus-harboring field isolate (w370) were transfected with purified rnmyrv-3 particles. virus infection was confirmed by electrophoresis and nor ... | 2007 | 18943646 |