| baculovirus expression of the 11 mycoreovirus-1 genome segments and identification of the guanylyltransferase-encoding segment. | the type member mycoreovirus 1 (myrv-1) of a newly described genus, mycoreovirus, isolated from a hypovirulent strain 9b21 of the chestnut blight fungus, has a genome composed of 11 dsrna segments (s1-s11). all of the segments have single orfs on their capped, positive-sense strands. infection of insect cells by baculovirus recombinants carrying full-length cdnas of s1-s11 resulted in overexpression of protein products of the expected sizes, based on their deduced amino acid sequences. this expr ... | 2007 | 17170467 |
| mycoreovirus 1 s4-coded protein is dispensable for viral replication but necessary for efficient vertical transmission and normal symptom induction. | rearrangements of two segments, s6 and s10, of mycoreovirus 1 (myrv1), a member of the family reoviridae, were previously shown to be induced at a high rate by the multifunctional protein p29 encoded by a distinct ssrna virus, the prototype hypovirus chv1-ep713 (sun and suzuki, rna 14, 2557-2571, 2008). here we report the occurrence of rearrangements of myrv1 s4, albeit at a very low frequency, in the absence of chv1 p29, resulting in internal 80-90% deletions of the open reading frame (orf) in ... | 2010 | 20006368 |
| rearrangements of mycoreovirus 1 s1, s2, and s3 induced by a multifunctional protein p29 encoded by the prototypic hypovirus chv1-ep713. | mycoreovirus 1 (myrv1), a member of the reoviridae family possessing a genome consisting of 11 dsrna segments (s1-s11), infects the chestnut blight fungus and reduces its virulence (hypovirulence). we have previously demonstrated reproducible induction of intragenic rearrangements of myrv1 s6 (s6l: almost full-length duplication) and s10 (s10ss: internal deletion of three fourths of the orf), mediated by a multifunctional protein p29 encoded by the prototype hypovirus (chv1-ep713) of the hypovir ... | 2011 | 21508189 |
| a host factor involved in hypovirus symptom expression in the chestnut blight fungus, cryphonectria parasitica. | the prototype hypovirus chv1-ep713 causes virulence attenuation and severe suppression of asexual sporulation and pigmentation in its host, the chestnut blight fungus, cryphonectria parasitica. we identified a factor associated with symptom induction in c. parasitica using a transformation of c. parasitica strain ep155 with a full-length cdna clone from a mild mutant virus strain, cys(72). this was accomplished by using mutagenesis of the transformant fungal strain tcys(72)-1 by random integrati ... | 2007 | 17977965 |
| mycoreovirus genome rearrangements associated with rna silencing deficiency. | mycoreovirus 1 (myrv1) has 11 double-stranded rna genome segments (s1 to s11) and confers hypovirulence to the chestnut blight fungus, cryphonectria parasitica. myrv1 genome rearrangements are frequently generated by a multifunctional protein, p29, encoded by a positive-strand rna virus, cryphonectria hypovirus 1. one of its functional roles is rna silencing suppression. here, we explored a possible link between myrv1 genome rearrangements and the host rna silencing pathway using wild-type (wt) ... | 2015 | 25800742 |
| synergism between a mycoreovirus and a hypovirus mediated by the papain-like protease p29 of the prototypic hypovirus chv1-ep713. | infection of the chestnut blight fungus, cryphonectria parasitica, by the prototypic hypovirus cryphonectria hypovirus 1-ep713 (chv1-ep713) or by the type member, mycoreovirus 1-cp9b21 (myrv1-cp9b21), of a novel genus (mycoreovirus) of the family reoviridae results in hypovirulence, but with a different spectrum of phenotypic changes. the former virus depresses pigmentation and conidiation dramatically, whilst the latter virus has little effect on these processes. this study showed that double i ... | 2006 | 17098988 |
| mycoreovirus genome alterations: similarities to and differences from rearrangements reported for other reoviruses. | the family reoviridae is one of the largest virus families with genomes composed of 9-12 double-stranded rna segments. it includes members infecting organisms from protists to humans. it is well known that reovirus genomes are prone to various types of genome alterations including intragenic rearrangement and reassortment under laboratory and natural conditions. recently distinct genetic alterations were reported for members of the genus mycoreovirus, mycoreovirus 1 (myrv1), and myrv3 with 11 (s ... | 2012 | 22675320 |
| intragenic rearrangements of a mycoreovirus induced by the multifunctional protein p29 encoded by the prototypic hypovirus chv1-ep713. | mycoreovirus 1 (myrv1), a member of the reoviridae family possessing a genome consisting of 11 dsrna segments (s1-s11), and the prototype hypovirus (chv1-ep713) of the hypoviridae family, which is closely related to the monopartite picorna-like superfamily with a ssrna genome, infect the chestnut blight fungus and cause virulence attenuation and distinct phenotypic alterations in the host. here, we present evidence for reproducible induction of intragenic rearrangements of myrv1 s6 and s10, medi ... | 2008 | 18945807 |