| antimicrobial susceptibility pattern of edwardsiella ictaluri isolates from natural outbreaks of bacillary necrosis of pangasianodon hypophthalmus in vietnam. | the purpose of this study was to assess the in vitro susceptibility of 64 vietnamese isolates of edwardsiella ictaluri, the causal agent of the infectious disease bacillus necrosis pangasius in pangasianodon hypophthalmus, using the agar dilution technique. all isolates originated from different farms and were collected between 2002 and 2005. none of the isolates displayed acquired resistance to amoxicillin, amoxicillin-clavulanic acid, chloramphenicol, florfenicol, gentamicin, kanamycin, neomyc ... | 2008 | 19090723 |
| comparison between a taqman polymerase chain reaction assay and a culture method for ctx-positive vibrio cholerae detection. | the main objective of the present work was to evaluate a real-time polymerase chain reaction (pcr) method to detect toxigenic vibrio cholerae in pangasius hypophthalmus, a freshwater fish cultured mainly in south east asia. a fda traditional culture method and a real-time pcr method of the ctx gene were used for detection of v. cholerae in spiked samples of pangasius fish. after an overnight enrichment of samples at 37 degrees c in alkaline peptone water, 2 cfu/25 g of fish was detected with bot ... | 2010 | 20229998 |
| effects of replacing fish meal with catfish (pangasius hypophthalmus) processing waste water on the performance of growing pigs. | a feeding trial with growing pigs was carried out in which fish meal was replaced by different levels of tra catfish (pangasius hypophthalmus) by-product processing waste water (ww). a control diet included fish meal (fm) as the sole protein supplement (ww0), and there were four experimental diets in which 100% (ww100), 75% (ww75), 50% (ww50), and 25% (ww25), respectively, of the crude protein from fm in ww0 was replaced by ww. thirty crossbred castrated (yorkshire × landrace) male pigs with an ... | 2010 | 20936347 |
| in vitro characterization of a recombinant ahl-lactonase from bacillus cereus isolated from a striped catfish (pangasianodon hypophthalmus) pond. | aiia gene encoding ahl-lactonase was isolated from bacillus cereus strain n26.2, originating from a striped catfish (pangasianodon hypophthalmus) pond in vietnam. this gene, abbreviated as aiia(n26.2), was cloned and expressed in a competent escherichia coli strain bl21(de3)plyss. the resulting protein, abbreviated as aiian26.2, was highly active in the ph range of 6-8 and could retain 80 % of the maximum activity under storage for 5 days at 4 °c or for 3 days at 20 °c. these properties of aiian ... | 2013 | 24426155 |
| effects of the dietary supplementation of mixed probiotic spores of bacillus amyloliquefaciens 54a, and bacillus pumilus 47b on growth, innate immunity and stress responses of striped catfish (pangasianodon hypophthalmus). | the study used the mixed probiotics of bacillus amyloliquefaciens 54a and b. pumilus 47b isolated from striped catfish (pangasianodon hypophthalmus) intestine aiming to stimulate growth performance, innate immunity, stress tolerance of striped catfish. the average weight gain (awg), specific growth rate (sgr), and feed conversion ratio (fcr) were analyzed after fish were fed the mixture of probiotics (b. amyloliquefaciens 54a and b. pumilus 47b) at concentrations of 1 × 10(8), 3 × 10(8), and 5 × ... | 2017 | 27836719 |
| gfp tagged vibrio parahaemolyticus dahv2 infection and the protective effects of the probiotic bacillus licheniformis dahb1 on the growth, immune and antioxidant responses in pangasius hypophthalmus. | in this study, the pathogenicity of gfp tagged vibrio parahaemolyticus dahv2 and the protective effect of the probiotic strain, bacillus licheniformis dahb1 was studied on the asian catfish, pangasius hypophthalmus. the experiment was carried out for 24 days with three groups and one group served as the control (without treatment). in the first group, p. hypophthalmus was orally infected with 1 ml of gfp tagged v. parahaemolyticus dahv2 at two different doses (10(5) and 10(7) cfu ml(-1)). in the ... | 2016 | 26952171 |
| optimisation and standardisation of functional immune assays for striped catfish (pangasianodon hypophthalmus) to compare their immune response to live and heat killed aeromonas hydrophila as models of infection and vaccination. | aquaculture production of pangasianodon hypophthalmus is growing rapidly in south east asia, especially in vietnam. as it is a relatively new aquaculture species there are few reports evaluating its immune response to pathogens. thus, functional assays for p. hypophthalmus were optimised to evaluate both innate and adaptive immune responses, and were then used to examine immune response following stimulation with live and heat-killed aeromonas hydrophila. these were used as models of infection a ... | 2014 | 25064539 |
| detection of quorum sensing signal molecules in edwardsiella ictaluri ei-151. | edwardsiella ictaluri is a gram-negative pathogenic bacterium in the family enterobacteriaceae that causes enteric septicemia of catfish, which has become a significant problem in the aquaculture of striped catfish (pangasianodon hypophthalmus) in vietnam. in this study, a bacterium designated as ei-151 was isolated from diseased striped catfish and proved to be virulent. based on 16s rdna sequencing and phenotypic tests, the pathogenic bacterium was identified as edw. ictaluri. the presence of ... | 2012 | 24293714 |
| serum carbohydrate-binding igm are present in vietnamese striped catfish (pangasianodon hypophthalmus) but not in north african catfish (clarias gariepinus). | pangasianodon hypophthalmus serum was fractionated by affinity chromatography on 12 different sepharose-carbohydrate columns and proteins eluted by the corresponding sugar. binding to the affinity matrices is dependent on ca(2+) ions. upon gel filtration using superose-12, essentially one fraction was obtained, eluting as a protein with a molecular mass of about 900 kda. sds-page in reducing conditions revealed the presence of large (72 kda) subunits (h-chains) and one up to three small (24, 26 ... | 2012 | 21911003 |
| experimental challenge studies in vietnamese catfish, pangasianodon hypophthalmus (sauvage), exposed to edwardsiella ictaluri and aeromonas hydrophila. | the two main diseases in the pangasius catfish industry are bacillary necrosis of pangasianodon (bnp) and motile aeromonas septicaemia (mas), where the aetiological agents have been identified as edwardsiella ictaluri and aeromonas hydrophila, respectively. in this study, apparently healthy pangasianodon hypophthalmus were exposed to e. ictaluri, a. hydrophila or both bacterial species by intraperitoneal injection or immersion. there were 20 fish per treatment group, and the bacterial isolates u ... | 2010 | 20572902 |
| isolation and characterization of sap and crp, two pentraxins from pangasianodon (pangasius) hypophthalmus. | from the serum of pangasianodon hypophthalmus, two proteins were isolated by affinity chromatography on sepharose and phosphorylcholine-sepharose. their binding on the affinity matrices critically depends on the presence of ca2+ ions. n-terminal sequencing and sequencing of internal tryptic peptides identified the proteins as pentraxins and from their binding properties they are identified as sap (serum amyloid p component) and crp (c-reactive protein). per ml serum, 36 microg sap and 56 microg ... | 2010 | 20109557 |
| adverse effects of enrofloxacin when associated with environmental stress in tra catfish (pangasianodon hypophthalmus). | the aim of this study was to assess the adverse effects of enrofloxacin (ef) on tra catfish, pangasianodon hypophthalmus, in relation with density stress. fish were held at 40, 80 or 120 fish m(-3) and fed with pellets containing either 1 g kg(-1) ef or no ef. antibiotic exposure lasted 7d and all fish were fed without ef for another 7-d recovery period. fish were sampled at 3, 7, 8, 10 and 14 d after the beginning of ef exposure. lipid peroxidation (lpo) and total glutathione (gsh) levels, cata ... | 2009 | 19836822 |
| combined immersion and oral vaccination of vietnamese catfish (pangasianodon hypophthalmus) confers protection against mortality caused by edwardsiella ictaluri. | edwardsiella ictaluri septicemia occurs worldwide and causes high mortality and considerable economic damage to the catfish industry especially in vietnam and the usa. to control edwardsiella septicemia farmers extensively use antibiotics and various vaccination methods. vaccination with inactivated vaccines has come with variable efficacy. in this trial the results of an approach of controlling edwardsiella septicemia of tra catfish (pangasianodon hypophthalmus) in vietnam through vaccination v ... | 2009 | 19747976 |
| occurrence of two myxosporean species, myxobolus hakyi sp. n. and hoferellus pulvinatus sp. n., in pangasianodon hypophthalmus fry imported from thailand to europe as ornamental fish. | fingerlings of the sutchi catfish pangasianodon hypophthalmus, a favorite food fish in south asia, is regularly imported by european fish traders and sold in pet fish shops. in shipments from thailand, a skin and a kidney infection of this fish caused by myxosporean parasites was found both in hungary and russia. in the skin of the fish, small millet-sized nodules containing great numbers of a myxobolus species were found, while in the renal glomeruli, spores and sporogonic stages of a hoferellu ... | 2009 | 19629523 |
| production and characterization of monoclonal antibodies specific to pangasius catfish, basa, and tra. | four igg (subclass igg1) class monoclonal antibodies (mabs) strongly reactive to asian farm-raised pangasius catfish, tra (pangasius hypophthalmus) and basa (pangasius bocourti), have been developed. these mabs were raised by immunizing an animal with thermal-stable crude sarcoplasmic protein extract of cooked tra. the mabs were selected by screening hybridoma clones against more than 70 common fish and meat protein extracts. two mabs, t7e10 and t1g11, were found to be specific to the asian pang ... | 2009 | 19397709 |
| status and future perspectives of vaccines for industrialised fin-fish farming. | fin fish farming is developing from extensive to intensive high industrial scale production. production of fish in high-density growth conditions requires effective vaccines in order to control persistent and emerging diseases. vaccines can also have significant positive impact on the reduced usage of antibiotics. this was demonstrated when vaccines were introduced in norway for atlantic salmon (salmo salar) in the late eighties and early nineties, resulting in a rapid decline of antibiotics con ... | 2013 | 23769873 |
| effect of garlic extract on physical, oxidative and microbial changes during refrigerated storage of restructured product from thai pangas (pangasianodon hypophthalmus) surimi. | the effect of garlic's aqueous extract (gae) during refrigerated storage of the restructured products from pangasius (pangasianodon hypophthalmus) was evaluated. protein and lipid oxidation, protein pattern on sds-page, tpc as well as whc, gelling properties, texture profiles and whiteness of the surimi gel was evaluated periodically during a refrigerated storage period of 20 days. increase of water holding capacity in gae added gels indicated stronger protein network formation, whereas, decreas ... | 2015 | 26604371 |
| genetic variation and population structure of endemic yellow catfish, horabagrus brachysoma (bagridae) among three populations of western ghat region using rapd and microsatellite markers. | random amplified polymorphic dna (rapd) and microsatellite markers were applied to evaluate the genetic variation in endemic and endangered yellow catfish, horabagrus brachysoma sampled from three geographic locations of western ghat, south india river systems. in rapd, of 32 10-mer rapd primers screened initially, 10 were chosen and used in a comparative analysis of h. brachysoma collected from meenachil, chalakkudy and nethravathi river systems. of the 124 total rapd fragments amplified, 49 (3 ... | 2009 | 18979230 |
| effects of lead, molybdenum, rubidium, arsenic and organochlorines on spermatogenesis in fish: monitoring at mekong delta area and in vitro experiment. | to estimate the influence of water contaminants on fish reproduction in the mekong delta area, we sampled cultivated male catfish (pangasianodon hypophthalmus), investigated testicular development, and measured persistent organic pollutants (pops) and trace element levels in muscle and liver, respectively. various testes sizes were observed although sampling took place during a short period. histological analysis revealed that all developmental stages of germ cells were observed in catfish with ... | 2007 | 17448548 |
| high genetic diversity in cryptic populations of the migratory sutchi catfish pangasianodon hypophthalmus in the mekong river. | the detection and conservation of spawning units is of crucial importance in highly migratory species. the sutchi catfish pangasianodon hypophthalmus (pangasiidae; teleostei) is a common large-sized tropical fish, which migrates annually to several upstream spawning sites on the lower mekong river and feeds on the huge floodplain of the lower mekong and tonle sap for the other half of the year. we hypothesised that because of the relative size of the feeding and spawning habitat, genetic variabi ... | 2006 | 16369576 |
| early interactions of edwardsiella ictaluri, with pangasianodon catfish and its invasive ability in cell lines. | commercial pangasianodon catfish production is heavily impacted by bacillary necrosis of pangasius (bnp) caused by edwardsiella ictaluri. this study aimed to investigate the early bacterium-host interactions following immersion challenge and to compare the retrieved data with the invasion ability of the used isolates in fish cell lines. firstly, pangasianodon hypophthalmus fingerlings were challenged via immersion using e. ictaluri isolate ho2 or 223. at different times post inoculation, fish we ... | 2012 | 22422551 |
| intraspecific diversity of edwardsiella ictaluri isolates from diseased freshwater catfish, pangasianodon hypophthalmus (sauvage), cultured in the mekong delta, vietnam. | a molecular epidemiology study was conducted on 90 edwardsiella ictaluri isolates recovered from diseased farmed freshwater catfish, pangasianodon hypophthalmus, cultured in the mekong delta, vietnam. thirteen isolates of e. ictaluri derived from diseased channel catfish, ictalurus punctatus, cultured in the usa were included for comparison. all the e.ictaluri isolates tested were found to be biochemically indistinguishable. a repetitive (rep)-pcr using the single (gtg)(5) primer was shown to po ... | 2012 | 22804634 |
| in vivo effects of escherichia coli lipopolysaccharide on regulation of immune response and protein expression in striped catfish (pangasianodon hypophthalmus). | lipolysaccharide (lps), a component of outer membrane protein of gram-negative bacteria, reportedly stimulates fish immune system. however, mechanisms driving this immunomodulatory effect are yet unknown. to determine effects of escherichia coli lipopolysaccharide on regulation of immune response and protein expression of striped catfish (pangasianodon hypophthalmus), juvenile fish (20-25 g) were injected with 3, 15 or 45 mg e.coli lps/kg and challenged with edwardsiella ictaluri. plasma cortiso ... | 2013 | 23207480 |
| can immunostimulants efficiently replace antibiotic in striped catfish (pangasianodon hypophthalmus) against bacterial infection by edwardsiella ictaluri? | the present study was performed to determine the efficacy of lipopolysaccharide (lps) and levamisole on immune response and disease resistance in striped catfish and to compare their respective efficiency with the one of an antibiotic treatment after infection of fish by the bacteria edwardsiella ictaluri. fish were divided into 3 groups and each group was injected with lps (3 mg/kg fish), levamisole (5 mg/kg fish) or phosphate buffer saline as control. at day 21st post immunostimulant injection ... | 2014 | 25120215 |
| the effects of feeding immunostimulant β-glucan on the immune response of pangasianodon hypophthalmus. | immunostimulants are food additives used by the aquaculture industry to enhance the immune response of fish, and although β-glucans are now commonly used for this purpose in aquaculture, little is known about their effects on the immune response of pangasianodon hypophthalmus. thus, a variety of immune parameters (e.g. phagocytosis, respiratory burst, lysozyme, complement, peroxidase, total protein, total anti-protease, total igm, natural antibody titres, and specific igm titres) was examined in ... | 2015 | 25921238 |
| the effects of feeding β-glucan to pangasianodon hypophthalmus on immune gene expression and resistance to edwardsiella ictaluri. | pangasianodon hypophthalmus (striped catfish) is an important aquaculture species and intensification of farming has increased disease problems, particularly edwardsiella ictaluri. the effects of feeding β-glucans on immune gene expression and resistance to e. ictaluri in p. hypophthalmus were explored. fish were fed 0.1% fungal-derived β-glucan or 0.1% commercial yeast-derived β-glucan or a basal control diet without glucan. after 14 days of feeding, the mrna expression of immune genes (transfe ... | 2015 | 26439415 |
| synergic stress in striped catfish (pangasianodon hypophthalmus, s.) exposed to chronic salinity and bacterial infection: effects on kidney protein expression profile. | hyperosmotic stress has often been investigated from osmoregulation perspectives while the effects of such stress on the immune capacity remain largely unexplored. in this study, striped catfish were submitted to a gradual hyperosmotic stress (up to 20ppt), followed by an infection with a virulent strain of an intracellular bacteria, edwardsiella ictaluri. physiological parameters (plasma osmolarity, gill nak atpase) have been investigated. moreover, a label free quantitative proteomics workflow ... | 2016 | 27155471 |
| effect of oligochitosan and oligo-β-glucan supplementation on growth, innate immunity, and disease resistance of striped catfish (pangasianodon hypophthalmus). | oligochitosan (cos) and oligo-β-glucan (βog) were prepared by gamma co-60 irradiation of chitosan/h2 o2 and β-glucan/h2 o2 solutions. the striped catfish (pangasianodon hypophthalmus) was fed diets containing 0-200 mg cos, βog, and a mixture of cos/βog per kg feed for 45 days, and then challenged with edwardsiella ictaluri bacterium. the effects of supplemented cos, βog, and a mixture of cos/βog on immune stimulation and growth performance in striped catfish were investigated. the results indica ... | 2016 | 27245476 |
| chronic hyperosmotic stress interferes with immune homeostasis in striped catfish (pangasianodon hypophthalmus, s.) and leads to excessive inflammatory response during bacterial infection. | hyperosmotic stress has often been investigated from osmoregulation perspectives while the effects of such stress on the immune capacity remain largely unexplored. in this study, striped catfish were submitted to three salinity profiles (freshwater, low saline water, saline water) during 20 days, followed by infection with a virulent bacteria, edwardsiella ictaluri, responsible for the enteric septicaemia of catfish. osmoregulatory (plasma osmolality, gill na(+)k(+)atpase), immune (blood cells, ... | 2016 | 27346159 |
| edwardsiella ictaluri infection in pangasius catfish imported from west bengal into the southern caribbean. | in response to a mortality event, seven pangasius catfish (pangasianodon hypophthalmus) were submitted to the university of the west indies, school of veterinary medicine, trinidad and tobago, for diagnostic evaluation. these fish were part of a consignment that arrived from kolkata two weeks earlier. fish presented with perianal haemorrhage and blister-like swellings on the skin which ruptured to leave ulcers. edwardsiella ictaluri was consistently recovered from the brain and skin. repetitive ... | 2016 | 27592704 |
| chronic hyperosmotic stress inhibits renal toll-like receptors expression in striped catfish (pangasianodon hypophthalmus, sauvage) exposed or not to bacterial infection. | toll-like receptors (tlrs) are the first innate receptors in recognizing pathogen-associated molecular patterns. in fish, upregulation of toll-like receptors during infection has been largely demonstrated while the effects of abiotic stressors on their expression remain poorly investigated. in this study, striped catfish were submitted during 20 days to three salinity profiles (freshwater, low saline water, saline water), followed by injection of a bacterial strain of edwardsiella ictaluri. the ... | 2017 | 28344170 |
| microbiological spoilage of vacuum and modified atmosphere packaged vietnamese pangasius hypophthalmus fillets. | this study investigated the identity, growth and metabolite production of micro-organisms causing spoilage of pangasius hypophthalmus fillets packaged in air, vacuum and modified atmospheres (map) (map 1: 50%co(2)-50%n(2) and map 2: 50%co(2)-50%o(2)) during storage at 4 °c. based on the time it took for psychrotrophic total colony counts to exceed 7 log cfu g(-1), the shelf life of the fillets packaged in air, vacuum, map 1 and map 2 was estimated to be 7, 10, 12 and 14 days respectively. the lo ... | 2012 | 22365354 |
| dietary zinc promotes immuno-biochemical plasticity and protects fish against multiple stresses. | the abiotic and biotic stress is an episode that effect on regulatory, neuro-endocrine and immune systems of animals including fish. the stress creates stimulatory and suppressive of immune system resulting in increases the incidence of infection. in view of these points, we have conducted an experiment to mitigate the stress through a nutritional approach through zinc (zn) supplementation in pangasius hypophthalmus (initial weight-3.65 ± 0.75 g). three isocaloric and isonitrogenous diets with g ... | 2017 | 28108338 |
| studies on expression pattern of toll-like receptor 5 (tlr5) in edwardsiella tarda infected pangasianodon hypophthalmus. | tlr5 is one of the important prr (pathogen recognition receptors) and plays a fundamental role in pathogen recognition and activation of innate immune responses. it recognizes bacterial flagellin and stimulates the production of proinflammatory cytokines, through signalling via the adaptor protein myd88. in this study, we characterized partial tlr5 (soluble form) gene from pangasianodon hypophthalmus and analysed its expression profile upon challenge by edwardsiella tarda. bioinformatic analysis ... | 2017 | 28159691 |
| first isolation and characterization of edwardsiella tarda from diseased striped catfish, pangasianodon hypophthalmus (sauvage). | | 2014 | 24344765 |
| decontamination of pangasius fish (pangasius hypophthalmus) with chlorine or peracetic acid in the laboratory and in a vietnamese processing company. | this study evaluated the decontamination of pangasius fillets in chlorine or peracetic acid treated wash water. first, the decontamination efficacy of the washing step with chlorinated water applied by a vietnamese processing company during trimming of pangasius fillets was evaluated and used as the basis for the experiments performed on a laboratory scale. as chlorine was only added at the beginning of the batch and used continuously without renewal for 239min; a rapid increase of the bacterial ... | 2015 | 26058007 |
| microbiological and chemical safety concerns regarding frozen fillets obtained from pangasius sutchi and nile tilapia exported to european countries. | microbiological and chemical safety concerns regarding frozen fillets from pangasius catfish (pangasius hypophthalmus) exported to poland, germany and ukraine and nile tilapia (oreochromis niloticus) exported to poland and germany were investigated by analyzing heavy metal residues, microbiological hazards, biogenic amines, and thiobarbituric acid (tba) and total volatile basic nitrogen (tvb-n) content. | 2016 | 25907121 |
| cloning and characterization of pangasianodon hypophthalmus growth hormone gene and its heterologous expression. | pangasianodon hypophthalmus is one of the fast-growing catfish of freshwater origin, and its growth is attributed by the action of growth hormone (gh). in this study, the growth hormone gene (phgh) of 3.0 kb was characterized, and it is composed of five exons and four introns and having characteristics of an upstream region that contains tata, caat boxes, and binding sites of important transcription factors like pit-1a, cre, creb, crebp, ap-1, sp1, and tbp. the full-length cdna sequence of 1,069 ... | 2014 | 24811733 |
| growth hormone from striped catfish (pangasianodon hypophthalmus): genomic organization, recombinant expression and biological activity. | growth hormone is an essential polypeptide required for normal growth and development of vertebrates. in this report, striped catfish (pangasianodon hypophthalmus) growth hormone gene and cdna were isolated by reverse transcriptase-polymerase chain reaction. the striped catfish growth hormone (scgh) encoding gene contains 5 exons and 4 introns. the cdna sequence of the scgh gene contains a 603bp open reading frame and encodes for a 200-aa protein consisting of a putative 22-aa signal peptide and ... | 2013 | 23353774 |
| first identification of flavobacterium columnare infection in farmed freshwater striped catfish pangasianodon hypophthalmus. | the bacterium flavobacterium columnare was recovered and identified as the aetiological agent causing freshwater columnaris infection in farmed striped catfish pangasianodon hypophthalmus (sauvage) fingerlings that had suffered high mortality rates within commercial hatchery ponds in vietnam. the gross clinical signs were typical of columnaris-infected fish. histological examination found numerous gram-negative, filamentous bacteria present on the skin, muscle and gill tissues of affected fish. ... | 2012 | 22885517 |
| detection of channel catfish virus in cage-cultured pangasius hypophthalmus (sauvage, 1878) in malaysia. | | 2014 | 24117659 |
| fish peripheral blood mononuclear cells preparation for future monitoring applications. | fish species possess many specific characteristics that support their use in ecotoxicology. widely used in clinical research, peripheral blood mononuclear cells (pbmcs) can reasonably be exploited as relevant target cells in the assessment of environmental chemical toxicity. the current article focuses on the methods necessary to isolate, characterize, and culture fish pbmcs. these procedures were successfully applied on an endangered species, the european eel (anguilla anguilla l.), and on an e ... | 2012 | 22497769 |
| myxozoan pathogens of malaysian fishes cultured in ponds and net-cages. | we describe new myxosporean species from malaysian fishes cultured in pond farms and net-cages. myxobolus omari sp. nov. and m. leptobarbi sp. nov. were found in the muscles of pangasianodon hypophthalmus and leptobarbus hoevenii, respectively, while plasmodia and spores of thelohanellus zahrahae sp. nov. and henneguya daoudi sp. nov. were detected in the gills of barbonymus gonionotus and trichogaster trichopterus, respectively. plasmodia and spores found in these fishes differed from the known ... | 2009 | 19301636 |
| optimizing de novo transcriptome assembly and extending genomic resources for striped catfish (pangasianodon hypophthalmus). | striped catfish (pangasianodon hypophthalmus) is a commercially important freshwater fish used in inland aquaculture in the mekong delta, vietnam. the culture industry is facing a significant challenge however from saltwater intrusion into many low topographical coastal provinces across the mekong delta as a result of predicted climate change impacts. developing genomic resources for this species can facilitate the production of improved culture lines that can withstand raised salinity condition ... | 2015 | 25979246 |
| evaluation of mercury levels in pangasius and cod fillets traded in sicily (italy). | predator fishes at the top of the aquatic food chain can accumulate large concentrations of metals and their consumption, consequently, makes a significant contribution, in particular, to mercury intake. the aim of this study was to determine mercury levels in fillets of two predatory species: pangasius (pangasius hypophthalmus) from the vietnam region of megong and chao pharayai and cod (gadus morhua) from the baltic and north sea, both being commercially important in the italian market. a comp ... | 2012 | 22575000 |
| modulation of innate immune responses and induction of oxidative stress biomarkers in pangasianodon hypophthalmus following an experimental infection with dactylogyrid monogeneans. | modulation of innate immune activity and oxidative stress response of pangasianodon hypophthalmus through experimental infection with (thaparocleidus sp.) dactylogyrid monogenean was studied. a standard cohabitation method was used to infect healthy experimental fish. after 14 days, dactylogyrid (gill monogenean) infested fish were sampled and categorised into three different infected groups namely (t1) low (<10 mean dactylogyrid per gill arch per fish), (t2) moderate (10-49 mean dactylogyrid pe ... | 2017 | 28232278 |
| myxozoan pathogens in cultured malaysian fishes. i. myxozoan infections of the sutchi catfish pangasius hypophthalmus in freshwater cage cultures. | cage-cultured sutchi catfish pangasius hypophthalmus (sauvage, 1878), a favourite food fish in southeast asia, proved to be infected by 6 myxozoan species. three species belonged to the genus hennegoides (h. berlandi, h. malayensis, and h. pangasii), 1 to henneguya (h. shariffi) and 2 to myxobolus (m. baskai, and m. pangasii). five myxozoans infected the gills and 1 was found on the spleen. myxozoans infecting the gills were characterised by a specific site selection. h. shariffi sp. n. and h. b ... | 2006 | 16610586 |
| isolation of heat-tolerant myoglobin from asian swamp eel monopterus albus. | myoglobin from asian swamp eel monopterus albus was purified from fish muscle using salt fractionation followed by column chromatography and molecular filtration. the purified mb of 0.68 mg/g wet weight of muscle was determined for its molecular mass by maldi-tof-ms to be 15,525.18 da. using isoelectric focusing technique, the purified mb showed two derivatives with pi of 6.40 and 7.12. six peptide fragments of this protein identified by lc-ms/ms were homologous to mbs of sea raven hemitripterus ... | 2012 | 22538454 |
| sixteen est-linked microsatellite markers in günther's walking catfish, clarias macrocephalus. | twenty-seven new microsatellite sequences were identified by screening 2029 expressed sequence tags from günther's walking catfish, clarias macrocephalus. sixteen loci were polymorphic with the number of alleles ranging from two to 16 per locus and the observed and expected heterozygosities ranging from 0.4667 to 0.9333 and from 0.427 to 0.8819 per locus, respectively. cross-species amplifications of all 16 primer pairs were tested in four other species of catfish including clarias gariepinus, p ... | 2008 | 21586027 |