| cloning of a human parvovirus by molecular screening of respiratory tract samples. | the identification of new virus species is a key issue for the study of infectious disease but is technically very difficult. we developed a system for large-scale molecular virus screening of clinical samples based on host dna depletion, random pcr amplification, large-scale sequencing, and bioinformatics. the technology was applied to pooled human respiratory tract samples. the first experiments detected seven human virus species without the use of any specific reagent. among the detected viru ... | 2005 | 16118271 |
| evidence of human coronavirus hku1 and human bocavirus in australian children. | undiagnosed cases of respiratory tract disease suspected of an infectious aetiology peak during the winter months. since studies applying molecular diagnostic assays usually report reductions in the number of undiagnosed cases of infectious disease compared to traditional techniques, we applied pcr assays to investigate the role of two recently described viruses, namely human coronavirus (hcov) hku1 and human bocavirus (hbov), in a hospital-based paediatric population. both viruses were found am ... | 2006 | 16257260 |
| detection of human bocavirus in japanese children with lower respiratory tract infections. | human bocavirus (hbov), a newly cloned human virus of the genus bocavirus, was detected by pcr from nasopharyngeal swab samples (8 of 318; 5.7%) collected from children with lower respiratory tract infections. hbov may be one of the causative agents of lower respiratory tract infections in young children. | 2006 | 16517912 |
| human bocavirus infection, canada. | human bocavirus was detected in 18 (1.5%) of 1,209 respiratory specimens collected in 2003 and 2004 in canada. the main symptoms of affected patients were cough (78%), fever (67%), and sore throat (44%). nine patients were hospitalized; of these, 8 (89%) were <5 years of age. | 2006 | 16704852 |
| human bocavirus in children. | | 2006 | 16710957 |
| human bocavirus found in children with lower respiratory tract infection. | | 2005 | 16785691 |
| human bocavirus: prevalence and clinical spectrum at a children's hospital. | molecular methods of pathogen discovery have recently led to the description of several new respiratory viruses. human bocavirus (hbov), a proposed member of the family parvoviridae, is one of the most recently described respiratory viruses. initial reports indicate that hbov is a common cause of respiratory tract infection in children. | 2006 | 16804840 |
| frequent detection of bocavirus dna in german children with respiratory tract infections. | in a substantial proportion of respiratory tract diseases of suspected infectious origin, the etiology is unknown. some of these cases may be caused by the recently described human bocavirus (hbov). the aim of this study was to investigate the frequency and the potential clinical relevance of hbov in pediatric patients. | 2006 | 16834781 |
| frequent detection of human rhinoviruses, paramyxoviruses, coronaviruses, and bocavirus during acute respiratory tract infections. | viruses are the major cause of pediatric acute respiratory tract infection (arti) and yet many suspected cases of infection remain uncharacterized. we employed 17 pcr assays and retrospectively screened 315 specimens selected by season from a predominantly pediatric hospital-based population. before the brisbane respiratory virus research study commenced, one or more predominantly viral pathogens had been detected in 15.2% (n = 48) of all specimens. the brisbane study made an additional 206 vira ... | 2006 | 16847968 |
| the association of newly identified respiratory viruses with lower respiratory tract infections in korean children, 2000-2005. | this study was performed to evaluate the associations of newly recognized viruses, namely, human metapneumovirus (hmpv), human coronavirus (hcov)-nl63, and human bocavirus (hbov) with lower respiratory tract infections (lrtis) in previously healthy children. | 2006 | 16886150 |
| real-time pcr assays for detection of bocavirus in human specimens. | the recently discovered human bocavirus (hbov) is the first member of the family parvoviridae, genus bocavirus, to be potentially associated with human disease. several studies have identified hbov in respiratory specimens from children with acute respiratory disease, but the full spectrum of clinical disease and the epidemiology of hbov infection remain unclear. the availability of rapid and reliable molecular diagnostics would therefore aid future studies of this novel virus. to address this, ... | 2006 | 16954253 |
| human bocavirus in french children. | human bocavirus (hbov), a new member of the genus bocavirus in the family parvoviridae, has been recently associated with respiratory tract infections. we report the epidemiologic and clinical features observed from a 1-year retrospective study of hbov infection in young children hospitalized with a respiratory tract infection. | 2006 | 16965707 |
| bocavirus infection in hospitalized children, south korea. | this study presents the first evidence of human bocavirus infection in south korean children. the virus was detected in 27 (8.0%) of 336 tested specimens, including 17 (7.5%) of 225 virus-negative specimens, collected from children with acute lower respiratory tract infection. | 2006 | 16965708 |
| detection of bocavirus dna in nasopharyngeal aspirates of a child with bronchiolitis. | we describe a case of bronchiolitis associated with the newly detected human bocavirus (hbov) in a child with a suspected noonan syndrome. this is the first report of a bronchiolitis probably linked to hbov that required intensive care while being accompanied by a congenital heart disease and a history of several episodes of severe respiratory symptoms. | 2007 | 16968654 |
| human bocavirus: developing evidence for pathogenicity. | | 2006 | 17041844 |
| human bocavirus infection in young children in the united states: molecular epidemiological profile and clinical characteristics of a newly emerging respiratory virus. | human bocavirus (hbov) is a newly identified human parvovirus that was originally identified in the respiratory secretions of children with respiratory tract disease. to further investigate the epidemiological profile and clinical characteristics of hbov infection, we screened infants and children <2 years of age (hereafter referred to as "children") for hbov. | 2006 | 17041854 |
| epidemiological profile and clinical associations of human bocavirus and other human parvoviruses. | human bocavirus (hbov) and parv4 are newly discovered human parvoviruses. hbov, which was first detected in respiratory samples, has a potential role in the development of human respiratory disease. the present study compared the frequencies, epidemiological profiles, and clinical backgrounds of hbov and parv4 infections with those of other respiratory virus infections, by evaluating diagnostic samples referred to the specialist virology laboratory (svl) at the royal infirmary of edinburgh (edin ... | 2006 | 17041855 |
| human bocavirus infection among children, jordan. | human bocavirus was detected in 57 (18.3%) of 312 children with acute respiratory infection (ari) who required hospitalization in jordan. it was also detected in 30 (21.7%) of 138 children with severe ari, in 27 (15.5%) of 174 with mild or moderate disease, and in 41 (72%) of 57 with other pathogens. | 2006 | 17073092 |
| human bocavirus in hospitalized children, south africa. | | 2006 | 17073104 |
| detection of human bocavirus in canadian children in a 1-year study. | human bocavirus was detected by pcr in 65 (5.1%) of 1,265 respiratory specimens collected in 2002 and 2003 from the stollery children's hospital from children <17 years of age. the spectrum of illness included upper respiratory infection, croup, bronchiolitis, and pneumonia with a prominence of cough and fever. | 2007 | 17122013 |
| prevalence of viral respiratory tract infections in children with asthma. | previous studies support a strong association between viral respiratory tract infections and asthma exacerbations. the effect of newly discovered viruses on asthma control is less well defined. | 2007 | 17140648 |
| severe pneumonia and human bocavirus in adult. | | 2006 | 17176591 |
| high prevalence of human bocavirus detected in young children with severe acute lower respiratory tract disease by use of a standard pcr protocol and a novel real-time pcr protocol. | the human bocavirus (hbov) was recently isolated from respiratory tract samples. within a study collective of children with severe lower respiratory tract disease, the patients testing positive for hbov (12.8%) had a higher rate of underlying cardiopulmonary disease. viral loads in respiratory tract specimens varied from 10(2) to 10(10) genome equivalents/ml. | 2007 | 17215343 |
| human bocavirus dna detected by quantitative real-time pcr in two children hospitalized for lower respiratory tract infection. | | 2007 | 17216422 |
| isolation of human bocavirus from swiss infants with respiratory infections. | human bocavirus (hbov) is a novel agent associated with respiratory symptoms in adults and children. we studied prospectively the first acute respiratory infection in a birth cohort of healthy neonates in switzerland. hbov was identified in 5 (4.5%) of 112 infants as young as 3 months of age. in 4 of the 5 infants, hbov was associated with other respiratory viruses. we conclude that hbov circulates in the community and is acquired early in life. | 2007 | 17259883 |
| spectrum of viruses and atypical bacteria in intercontinental air travelers with symptoms of acute respiratory infection. | respiratory infections after air travel are frequent, but epidemiological data are incomplete. using sensitive polymerase chain reactions, we studied the spectrum of atypical bacteria and respiratory viruses in travelers fulfilling the case definition of severe acute respiratory syndrome. a pathogen was identified in 67 travelers (43.2%). influenza and parainfluenza viruses were most prevalent, at 14.2% and 15.5%, respectively. prevalences of adenoviruses, human metapneumovirus, coronaviruses, a ... | 2007 | 17262708 |
| quantification of human bocavirus in lower respiratory tract infections in china. | a quantitative pcr method was established to quantify human bocavirus (hbov) genomic copies in clinical specimens from children with lower respiratory tract infections (lrti) in china. a total of 257 respiratory tract specimens were tested, and 7 (2.7%) of these (all sputum samples) were positive, with genomic copies that ranged from 8.0 x 10(3) to 8.0 x 10(9) in the samples. the main clinical symptom of patients who were positive for hbov dna was a pneumonia-like syndrome represented by high fe ... | 2007 | 17266760 |
| human bocavirus: a novel parvovirus epidemiologically associated with pneumonia requiring hospitalization in thailand. | we detected human bocavirus (hbov) infection in 4.5% of hospitalized patients with pneumonia in rural thailand. however, the role of hbov as a pathogen is unclear. | 2007 | 17330795 |
| human bocavirus in italian patients with respiratory diseases. | hbov, a recently discovered parvovirus, can be present in the respiratory tract of patients with acute respiratory diseases (ard), but its etiologic involvement in the underlying diseases is still uncertain. | 2007 | 17336143 |
| human bocavirus and acute wheezing in children. | human bocavirus is a newly discovered parvovirus. it has been detected primarily in children with acute lower respiratory tract infection, but its occurrence, clinical profile, and role as a causative agent of respiratory tract disease are not clear. | 2007 | 17342639 |
| human bocavirus: a new viral pathogen. | | 2007 | 17342640 |
| human bocavirus infection, people's republic of china. | a newly identified parvovirus, human bocavirus (hbov), was found in 21 (8.3%) of 252 nasopharyngeal aspirates from hospitalized children with lower respiratory tract infection in hunan province, people's republic of china. viral loads were 10(4) to 10(10) copies/ml. phylogenetic analysis of the vp1 gene showed a single genetic lineage of hbov worldwide. | 2007 | 17370538 |
| human bocavirus in febrile children, the netherlands. | | 2007 | 17370546 |
| human bocavirus in iranian children with acute respiratory infections. | human bocavirus (hbov), a virus discovered in sweden in 2005, has been associated with acute respiratory infections in young children and subsequent reports suggest that hbov may have a worldwide distribution. this report describes the frequency and clinical presentation of hbov in 261 iranian children<5 years old with acute respiratory infections attending two regional hospitals in rasht, iran in the winter of 2003-2004. polymerase chain reaction (pcr) and reverse transcription pcr (rt-pcr) wer ... | 2007 | 17385723 |
| comparison of tissue distribution, persistence, and molecular epidemiology of parvovirus b19 and novel human parvoviruses parv4 and human bocavirus. | parv4 and human bocavirus (hbov) are newly discovered human parvoviruses with poorly understood epidemiologies and disease associations. we investigated the frequencies of persistence, tissue distribution, and influence of immunosuppression on replication of these viruses. | 2007 | 17397006 |
| community respiratory virus infections in immunocompromised patients: hematopoietic stem cell and solid organ transplant recipients, and individuals with human immunodeficiency virus infection. | infection is the leading cause of morbidity and mortality in immunocompromised patients such as hematopoietic/solid organ transplant recipients and individuals with human immunodeficiency virus. community respiratory virus infections are increasingly recognized as a significant threat to these patients. this article reviews current information in the clinical field of community respiratory viruses, including several newly discovered respiratory viruses. respiratory syncytial virus, influenza vir ... | 2007 | 17458776 |
| real-time pcr for diagnosis of human bocavirus infections and phylogenetic analysis. | the human bocavirus (hbov) was first described in 2005 in respiratory tract samples. the clinical relevance of hbov is still unclear. the aim of our study was to establish a real-time pcr assay for the detection and quantification of hbov dna, to apply the real-time assay for the analysis of stool and serum samples for the presence of hbov dna, and to perform a phylogenetic analysis of the hbov positive samples. a total of 834 nasopharyngeal aspirates (npa), 10 serum samples, and 31 stool sample ... | 2007 | 17475762 |
| [human bocavirus: a new respiratory pathogen?]. | | 2007 | 17506265 |
| two cases of severe obstructive pneumonia associated with an hku1-like coronavirus. | during the last few years a number of previously undescribed viruses, including human metapneumovirus, coronaviruses sars, nl63 and hku1, and bocavirus, were identified in nasopharyngeal samples from patients with signs of respiratory infections. these viruses may cause mild to life-threatening infections. | 2007 | 17507310 |
| frequent detection of the parvoviruses, parv4 and parv5, in plasma from blood donors and symptomatic individuals. | plasma pools used in the manufacture of blood- and plasma-derived medicinal products are frequently contaminated with parvovirus b19. the presence of the novel human parvovirus parv4 and a related variant parv5 in manufacturing plasma pools was recently demonstrated. another recently identified parvovirus, human bocavirus (hbov), has been identified in respiratory samples from children with lower respiratory tract disease. | 2007 | 17524097 |
| complete coding sequences and phylogenetic analysis of human bocavirus (hbov). | human bocavirus (hbov) is a novel virus which can cause respiratory tract disease in infants or children. recently, the prevalence of this virus has been studied worldwide. in this study, 18 of 252 (7.14%) nasopharyngeal suctions from infants or children between 1 month and 9 years of age with respiratory tract illness were hbov-positive by pcr. three positive samples were selected for sequencing the entire coding sequences using a new conserved set of primers. the results showed that the most c ... | 2007 | 17532505 |
| human bocavirus, a respiratory and enteric virus. | in spain, human bocavirus (hbov) was detected in 48 (9.1%) of 527 children with gastroenteritis at similar frequency as for children with respiratory illness (40/520, 7.7%). fecal excretion adds new concern about the transmission of hbov. to our knowledge, this report is the first to document hbov in human feces. | 2007 | 17553287 |
| detection of viruses identified recently in children with acute wheezing. | the etiologic role of recently identified respiratory viruses for acute wheezing in children is not yet clear. the purpose of this study was to investigate the prevalence of recently identified viruses, including human metapneumovirus (hmpv), human bocavirus (hbov), human coronavirus nl63 (hcov-nl63), and human coronavirus hku1 (hcov-hku1) in children with acute wheezing. viral etiology was identified in 231 children hospitalized with acute wheezing, aged from 1 month to 5 years. viral antigens ... | 2007 | 17597481 |
| human bocavirus in children with acute asthma. | | 2007 | 17599330 |
| [the human bocavirus: pathogen in airway infections?]. | the human bocavirus (hbov), the second member of the parvovirus family, which displays pathogenicity in humans, has been described in 2005 by allander et al.. it seems to be distributed worldwide and has been isolated mainly in infants and children with respiratory tract infection. this review covers all studies published on hbov to february 2007 and discusses this emerging viral pathogen from the perspective of inpatient medical treatment centers. | 2007 | 17607653 |
| electron microscopy observation of human bocavirus (hbov) in nasopharyngeal samples from hbov-infected children. | the newly identified human bocavirus is frequently detected by molecular techniques in respiratory samples from children with respiratory tract infections, but virions have not been observed so far. we report the electron microscopy observation of viral particles in nasopharyngeal aspirates previously found to be positive for human bocavirus dna. the virions presented the expected structural characteristics of a parvoviridae family member. | 2007 | 17652469 |
| [clinical characteristics of bocavirus infection among children]. | to study the clinical characteristics of human bocavirus (hbov) among children and to understand the association of hbov with human diseases. | 2007 | 17653317 |
| human bocavirus detection in an atopic child affected by pneumonia associated with wheezing. | human bocavirus (hbov) is a newly discovered human parvovirus. hbov was detected in respiratory samples by pcr, but its aetiologic role in the pathogenesis of acute respiratory infectious diseases is still unclear. | 2007 | 17686654 |
| newly discovered respiratory viruses: significance and implications. | with the recent advances in molecular biology and the ability to amplify viral genomes in a non-sequence-dependent manner, several previously unidentified human respiratory viruses have been discovered. there are accumulating data that some of these new pathogens are responsible for a substantial proportion of respiratory tract diseases, particularly in children. this review will focus on several of these newly identified pathogens for which there are clinical data implicating a role of these vi ... | 2007 | 17689145 |
| seroepidemiology of human bocavirus in hokkaido prefecture, japan. | a new human virus, provisionally named human bocavirus (hbov), was discovered by swedish researchers in 2005. a new immunofluorescence assay using trichoplusia ni insect cells infected with a recombinant baculovirus expressing the vp1 protein of hbov was developed, and the levels of immunoglobulin g antibody to the vp1 protein of hbov in serum samples were measured. the overall seroprevalence rate of antibodies against the vp1 protein of hbov in a japanese population aged from 0 months to 41 yea ... | 2007 | 17699639 |
| the transcription profile of the bocavirus bovine parvovirus is unlike those of previously characterized parvoviruses. | the bocavirus bovine parvovirus generated a single pre-mrna from a promoter at its left-hand end; however, the pattern of its alternative polyadenylation and splicing was different from that of other parvoviruses. a large left-hand-end open reading frame (orf) encoded a nonstructural protein of approximately 95 kda. an abundant, spliced, internally polyadenylated transcript encoded the viral np1 protein from an orf in the center of the genome. transcripts encoding the capsid proteins were polyad ... | 2007 | 17715221 |
| human bocavirus: multisystem detection raises questions about infection. | | 2007 | 17763314 |
| clinical and molecular epidemiology of human bocavirus in respiratory and fecal samples from children in hong kong. | human bocavirus (hbov) is a recently discovered parvovirus associated with respiratory tract infections in children. we conducted the first systematic prospective clinical and molecular study using nasopharyngeal aspirates (npas) and fecal samples. | 2007 | 17763318 |
| detection of human bocavirus in children hospitalized because of acute gastroenteritis. | the idea that human bocavirus (hbov) infection possibly plays a role in gastroenteritis has been suggested because of the frequent manifestation of gastrointestinal symptoms. the purpose of this study was to investigate the prevalence of hbov in children with gastroenteritis. we studied the etiologic agents in 962 children hospitalized with gastroenteritis. viral etiologic agents were detected by enzyme-linked immunosorbent assay or reverse-transcriptase polymerase chain reaction. a viral agent ... | 2007 | 17763319 |
| [human bocavirus infections in spanish 0-14 year-old: clinical and epidemiological characteristics of an emerging respiratory virus]. | in 2005 a new respiratory virus, called human bocavirus (hbov), was cloned from respiratory samples from swedish infants and children with lower respiratory tract infections. | 2007 | 17785157 |
| clinical features and complete genome characterization of a distinct human rhinovirus (hrv) genetic cluster, probably representing a previously undetected hrv species, hrv-c, associated with acute respiratory illness in children. | although human rhinoviruses (hrvs) are common causes of respiratory illness, their molecular epidemiology has been poorly investigated. despite the recent findings of new hrv genotypes, their clinical disease spectrum and phylogenetic positions were not fully understood. in this study, 203 prospectively collected nasopharyngeal aspirates (npas), negative for common respiratory viruses (83 were human bocavirus [hbov] positive and 120 hbov negative), from hospitalized children during a 1-year peri ... | 2007 | 17804649 |
| newly identified respiratory viruses. | | 2007 | 17848889 |
| detection of human bocavirus in children with kawasaki disease. | human bocavirus (hbov) is an emerging virus that has been implicated as a cause of acute upper and lower respiratory tract infection in children. as no serological assay is available, pcr was used to screen nasopharyngeal, serum or stool samples from 16 patients with kawasaki disease for hbov nucleic acid. hbov was identified by pcr in five (31.2%) patients, suggesting that this emerging virus may also play a pathogenic role in some cases of kawasaki disease. | 2007 | 17850342 |
| prospective study of human bocavirus (hbov) infection in a pediatric university hospital in germany 2005/2006. | human bocavirus (hbov), a new species of the genus parvovirus newly detected in 2005, seems to be a worldwide distributed pathogen among children with respiratory tract infection (prevalence 2%-18%). recently published retrospective studies and one prospective birth cohort study suggest that hbov-primary infection occurs in infants. | 2007 | 17851126 |
| [sequence analysis of the complete genome of bocavirus wll-1]. | human bocavirus, which was firstly discovered in 2005, is a new human parvovirus associated with lower respiratory tract infection in children. in this study, a human bocavirus, named wll-1 isolate, was identified in wenlin county, zhejiang province. the genome of bocavirus wll-1 has been sequenced and analyzed. phylogenetic analyses showed that wll-1 shares 99% homology with other bocaviruses recently reported, but also has some special variations. | 2007 | 17886722 |
| human bocavirus infection in hospitalized children during winter. | human bocavirus (hbov) has recently been described as a common agent of acute upper and lower respiratory tract infections in children. we screened by polymerase chain reaction for hbov nucleic acid nasopharyngeal aspirates from hospitalized children with negative culture and immunofluorescence assay for respiratory syncytial virus, influenza viruses, adenovirus, and parainfluenza viruses. hbov was detected in 32 children (5.5%) and was the second virus identified in nasopharyngeal aspirates aft ... | 2007 | 17901806 |
| high incidence of human bocavirus infection in children in spain. | the newly identified human bocavirus (hbov), a member of the parvoviridae family, has been associated to low respiratory tract infections in young children. | 2007 | 17904416 |
| high rate of human bocavirus and adenovirus coinfection in hospitalized israeli children. | we investigated coinfection of human bocavirus (hbov) and other respiratory viruses in hospitalized children by real-time pcr. a high rate (69.2%) of adenovirus infection was found among children infected with hbov. such high rates of hbov-adenovirus coinfection have not been previously reported, underscoring the need to investigate the contribution of hbov in patient clinical presentations. | 2008 | 17977996 |
| phospholipase a2-like activity of human bocavirus vp1 unique region. | human bocavirus (hbov) is a new parvovirus first discovered in 2005, which is associated with acute respiratory infection. analysis of sequence homology has revealed that a putative phospholipase a2 (pla2) motif exists in the vp1 unique region of hbov. however, little is known about whether the vp1 unique region of hbov has pla2 enzymatic activity and how these critical residues contribute to its pla2 activity. to address these issues, the vp1 unique region protein and four of its mutants, were ... | 2008 | 17981142 |
| no evidence for an association between infections with wu and ki polyomaviruses and respiratory disease. | wu virus (wuv) and ki polyomavirus (kipyv) are newly discovered related human polyomaviruses detected in respiratory samples. to investigate their potential role in respiratory disease, we determined their frequencies of detection, clinical presentations and epidemiological characteristics among samples referred for diagnostic respiratory virus testing. | 2007 | 17997354 |
| [recent progress in studies on human bocavirus]. | | 2007 | 18021561 |
| human bocavirus in children with acute lymphoblastic leukemia. | a new human parvovirus, human bocavirus, has recently been identified in respiratory secretions, feces and serum. it is associated with lower and most likely also upper respiratory tract infections. most commonly reported symptoms are cough, rhinorrhea, expiratory wheezing and fever, and the virus is preferentially detected in young children. we report three children with acute lymphoblastic leukemia who had acute febrile episodes with concomitant detection of human bocavirus in their respirator ... | 2008 | 18038236 |
| detection of human bocavirus in asturias, northern spain. | | 2008 | 18038242 |
| molecular epidemiology and disease severity of respiratory syncytial virus in relation to other potential pathogens in children hospitalized with acute respiratory infection in jordan. | human respiratory syncytial virus (hrsv) is the major viral cause of acute lower respiratory tract infections in children. few data about the molecular epidemiology of respiratory syncytial virus in developing countries, such as jordan, are available. the frequency and severity of infections caused by hrsv were assessed in hospitalized jordanian children <5 years of age compared with other potential etiological agents. overall a potential pathogen was detected in 78% (254/326) of the children. h ... | 2008 | 18041044 |
| human bocavirus. | human bocavirus (hbov) was first described in 2005 in nasopharyngeal aspirates of children with respiratory tract infection. multiple studies have confirmed the presence of hbov in respiratory tract samples of children world-wide. hbov has recently also been detected in blood and fecal samples. most studies so far have studied virus prevalence, and only a few reports provide data regarding the linkage of hbov to disease. these reports indicate that hbov infection is indeed associated with acute ... | 2008 | 18055252 |
| human bocavirus commonly involved in multiple viral airway infections. | human bocavirus (hbov) was recently discovered in children with acute respiratory tract infections. we have included a pcr for hbov in a study on airway infections in children. | 2008 | 18069054 |
| genotyping of human bocavirus using a restriction length polymorphism. | sequencing analysis of the isolates of a recently identified pathogen associated with respiratory infections, human bocavirus (hbov), allowed for identification of two virus genotypes of the virus. in the current article a new method for a simple and fast differentiation of hbov genotypes in clinical materials is described. the test includes an amplification of a 309 bp fragment of vp1/vp2 gene of hbov from nasopharyngeal aspirates with a subsequent incubation of a pcr mix with the bstapi endonu ... | 2008 | 18071891 |
| the human bocavirus role in acute respiratory tract infections of pediatric patients as defined by viral load quantification. | the major objective of this study was to investigate the pathogenic role of human bocavirus (hbov) in patients hospitalized with acute respiratory tract infection (arti). overall, 685 respiratory samples from 426 patients were examined by pcr for human bocavirus, as well as for other known human respiratory viruses. hbov was quantified by pcr. forty/283 (14.1%) pediatric patients, and 2/143 (1.4%) adult patients were found to harbor hbov for a total of 45 episodes (16 detected as single infectio ... | 2007 | 18080673 |
| [genome cloning and phylogenetic analysis of human bocavirus capsid gene]. | the full-length genome of one human bocavirus (hbov) and the vp1 sequences of nine hbov were amplified from patients' samples by pcr, cloned into pgem-t vector separately, and sequenced. in this study, the one full length gemome and nine vp1 sequences of hbov were aligened with 14 sequences of parvoviruses which were canonical exemplars in parvovirinae. phylogenetic analysis showed that hbov capsid sequences positioned closely to b19 parvovirus, although they positioned far in phylogenetic tree ... | 2007 | 18092681 |
| [epidemiology of acute upper and lower respiratory tract infections in children]. | more than 200 antigenically distinct viruses have been documented as causes of sporadic or epidemic respiratory infections in infants and children. the lung itself is rarely sampled directly, and sputum representing lower-airway secretions can rarely be obtained from children. in addition culture of secretions from upper respiratory tract is not useful since the normal flora includes the bacteria commonly responsible for pneumonia. clinical and radiology data only suggest the diagnosis. the deve ... | 2007 | 18092718 |
| [techniques used for the diagnostic of upper and lower respiratory tract viral infections]. | techniques used for the diagnostic of upper and lower respiratory tract viral infections techniques used for the diagnostic of upper and lower respiratory tract viral infections include conventional procedures and the new molecular tools. serology has no interest for diagnosis. antigen detection can be carried out by simple, easy and cheap tools. they are available at hospital and in community practice as well for the detection of influenza virus, rsv, hmpv, parainfluenza virus and adenovirus. t ... | 2007 | 18095623 |
| respiratory virus surveillance and outbreak investigation. | sensitive, rapid detection of respiratory viruses is needed for surveillance and for investigation of epidemiologically linked cases. the utility of rapid antigen-based methods for detection of common respiratory viruses and to confirm the cause of outbreaks is well established. however, nucleic acid amplification tests (nats) offer some benefits above antigen or culture-based procedures, with the main advantages being sensitivity and range of pathogens detectable. it is important to understand ... | 2007 | 18162251 |
| human bocavirus (hbov) in thailand: clinical manifestations in a hospitalized pediatric patient and molecular virus characterization. | human bocavirus (hbov), a novel virus, which based on molecular analysis has been associated with respiratory tract diseases in infants and children have recently been studied worldwide. to determine prevalence, clinical features and perform phylogenetic analysis in hbov infected thai pediatric patients. | 2008 | 18164764 |
| viral etiology of acute respiratory infections with cough in infancy: a community-based birth cohort study. | acute respiratory infections (ari) are a major cause of morbidity in infancy worldwide, with cough and wheeze being alarming symptoms to parents. we aimed to analyze in detail the viral aetiology of ari with such symptoms in otherwise healthy infants, including rhinoviruses and recently discovered viruses such as human metapneumovirus (hmpv), coronavirus nl63 and hku1, and human bocavirus (hbov). | 2008 | 18174876 |
| human bocavirus: clinical significance and implications. | human bocavirus (hbov), a parvovirus, was discovered in 2005 with the use of nonspecific genome amplification techniques. since its discovery, hbov has been identified worldwide. this review will focus on the epidemiology and clinical features associated with hbov infection. | 2008 | 18197041 |
| serodiagnosis of human bocavirus infection. | a new human-pathogenic parvovirus, human bocavirus (hbov), has recently been discovered and associated with respiratory disease in small children. however, many patients have presented with low viral dna loads, suggesting hbov persistence and rendering polymerase chain reaction-based diagnosis problematic. moreover, nothing is known of hbov immunity. we examined hbov-specific systemic b cell responses and assessed their diagnostic use in young children with respiratory disease. | 2008 | 18199037 |
| steps towards serological diagnosis of human bocavirus infections. | | 2008 | 18199041 |
| [clinical characteristics of 12 persistently wheezing children with human bocavirus infection]. | the impact of human bocavirus (hbov), a newly identified human parvovirus, on childhood persistent wheezing has not been identified. in this study, the clinical features of infantile persistent wheezing induced by hbov was analyzed. | 2007 | 18211753 |
| human bocavirus infection in children with gastroenteritis, brazil. | human bocavirus (hbov) was detected in 14 (2%) of 705 fecal specimens from brazilian children with gastroenteritis. coinfection with rotavirus, adenovirus, or norovirus was found in 3 (21.4%) hbov-positive specimens. none of the hbov-positive patients had respiratory symptoms. | 2007 | 18217564 |
| human bocavirus in infants, new zealand. | | 2007 | 18217577 |
| disseminated bocavirus infection after stem cell transplant. | | 2007 | 18252130 |
| human bocavirus and gastroenteritis. | | 2007 | 18258026 |
| human bocavirus infections in hospitalized children and adults. | studies have reported human bocavirus (hbov) in children with respiratory tract infections (rtis), but only occasionally in adults. we searched for hbov dna in nasopharyngeal aspirates (npas) from adults with exacerbations of chronic bronchitis or pneumonia, from children hospitalized for acute rtis, and from asymptomatic children during the winter of 2002-2003 in canada. hbov was detected in npas of 1 (0.8%) of 126 symptomatic adults, 31 (13.8%) of 225 symptomatic children, and 43 (43%) of 100 ... | 2008 | 18258113 |
| impact of human bocavirus on children and their families. | this study was planned to investigate the prevalence and clinical features of the illnesses associated with human bocavirus (hbov) in children with acute disease. we prospectively enrolled all subjects aged less than 15 years attending an emergency room in milan, italy, on wednesdays and sundays between 1 november 2004 and 31 march 2005 for any acute medical reason, excluding surgical diseases and trauma. nasopharyngeal swabs were collected at admission to detect hbov; influenza a and b viruses; ... | 2008 | 18287315 |
| elisas using human bocavirus vp2 virus-like particles for detection of antibodies against hbov. | human bocavirus (hbov) has been identified worldwide in children with lower respiratory tract infections with an incidence of approximately 2-11%. the role of hbov in pathogenesis, however, is largely unknown, and little is known about the epidemiology of the virus. to study the seroepidemiology of hbov infection, the capsid protein was expressed in insect cells. expression of the putative major capsid protein vp2 in insect cells led to the formation of virus-like particles exhibiting the typica ... | 2008 | 18289709 |
| pediatric hospitalization of acute respiratory tract infections with human bocavirus in hong kong. | respiratory tract infections are a leading cause of morbidity and mortality globally. human bocavirus (hbov) has recently been identified and implicated as an aetiologic agent of lower respiratory tract infection in children. | 2008 | 18296108 |
| two-year prospective study of single infections and co-infections by respiratory syncytial virus and viruses identified recently in infants with acute respiratory disease. | a prospective 2-year analysis including 322 infant patients with acute respiratory disease (ard) hospitalized in a pediatric department in northern italy was carried out to evaluate the role as respiratory pathogens or co-pathogens of recently identified viruses. the presence of respiratory syncitial virus (rsv), human metapneumoviruses (hmpvs), human bocaviruses (hbovs), and human coronaviruses (hcovs) was assayed by molecular detection and clinical symptoms evaluated. nasopharyngeal aspirates ... | 2008 | 18297694 |
| undiagnosed respiratory viruses in children. | an estimated 12 to 32 million upper respiratory infections occur in young children each year. in addition, 20% to 53% of infants will have > or = 1 episode of lower respiratory infection in the first year of life. the current methods of diagnosing respiratory viruses are limited in scope and sensitivity. polymerase chain reaction is a more sensitive method than antigen detection and is often used for newly discovered viruses. using polymerase chain reaction, we sought to diagnose adenoviruses, h ... | 2008 | 18310182 |
| human bocavirus detection in nasopharyngeal aspirates of children without clinical symptoms of respiratory infection. | the main objective of our study was to determine the frequency of human bocavirus (hbov) detection in asymptomatic children and to compare it with that in children hospitalized because of respiratory infection. hbov was detected in 5% of 116 healthy children versus 17% of 908 hospitalized children. hbov can be detected in healthy children but with a significantly lower frequency than in ill children. | 2008 | 18316984 |
| [clinical prospective study on maternal-fetal transmission of human bocavirus]. | to investigate maternal-fetal transmission at human bocavirus (hbov). | 2007 | 18322593 |
| [human bocavirus (hbov)]. | the human bocavirus (hbov) has been recently identified by means of molecular screening techniques in respiratory tract secretions from children with acute respiratory tract disease. this virus, which belongs to the parvoviridae family, has been detected worldwide with a 5 to 10% prevalence among children with upper or lower respiratory tract infections, essentially during the winter period. a seroepidemiological study has shown that almost all the children have antibodies to hbov by the age of ... | 2009 | 18343604 |
| respiratory viruses in hiv-infected patients with suspected respiratory opportunistic infection. | to assess the incidence and epidemiological pattern of respiratory viruses in hiv-infected patients and to evaluate their potential clinical impact. | 2008 | 18356599 |
| role of human metapneumovirus, human coronavirus nl63 and human bocavirus in infants and young children with acute wheezing. | the role of the novel respiratory viruses, human metapneumovirus (hmpv), human coronavirus nl63 (hcov nl63) and human bocavirus (hbov), in wheezing illness in children has not been well studied, especially in africa. the aim of this study was to investigate the prevalence of hmpv, hcov nl63 and hbov in south african children with acute wheezing. a prospective study of consecutive children presenting with acute wheezing to a pediatric hospital from may 2004 to november 2005 was undertaken. a nasa ... | 2008 | 18360904 |
| analysis of synonymous codon usage in 11 human bocavirus isolates. | human bocavirus (hbov) is a novel virus which can cause respiratory tract disease in infants or children. in this study, the codon usage bias and the base composition variations in the available 11 complete hbov genome sequences have been investigated. although, there is a significant variation in codon usage bias among different hbov genes, codon usage bias in hbov is a little slight, which is mainly determined by the base compositions on the third codon position and the effective number of cod ... | 2008 | 18378386 |
| development of real-time pcr assays for detection and quantification of human bocavirus. | human bocavirus (hbov) is a parvovirus that has been recently detected in patients with respiratory illness. | 2008 | 18378492 |